Life Sciences Commons^™

Sensitization Of Staphylococcus Aureus And Escherichia Coli To Antibiotics By The Sesquiterpenoids Nerolidol, Farnesol, Bisabolol, And Apritone, Byron F. Brehm-Stecher, Eric A. Johnson

Byron F. Brehm-Stecher

The sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone were investigated for their abilities to enhance bacterial permeability and susceptibility to exogenous antimicrobial compounds. Initially, it was observed by flow cytometry that these sesquiterpenoids promoted the intracellular accumulation of the membrane-impermeant nucleic acid stain ethidium bromide by live cells of Lactobacillus fermentum, suggesting that enhanced permeability resulted from disruption of the cytoplasmic membrane. The ability of these sesquiterpenoids to increase bacterial susceptibility to a number of clinically important antibiotics was then investigated. In disk diffusion assays, treatment with low concentrations (0.5 to 2 mM) of nerolidol, bisabolol, or apritone enhanced the susceptibility …

Multiple Luteinizing Hormone Receptor (Lhr) Protein Variants, Interspecies Reactivity Of Anti-Lhr Mab Clone 3b5, Subcellular Localization Of Lhr In Human Placenta, Pelvic Floor And Brain, And Possible Role For Lhr In The Development Of Abnormal Pregnancy, Pelvic Floor Disorders And Alzheimer's Disease, A Bukovsky, K Indrapichate, H Fujiwara, Maria Cekanova Ms, Rndr, Phd, Me Ayala, R Dominguez, Mr Caudle, J Wimalsena, Rf Elder, P Copas, Jf Foster, Ri Fernando, Dc Henley, Nb Upadhyaya

Maria Cekanova MS, RNDr, PhD

Distinct luteinizing hormone receptor (LHR) protein variants exist due to the posttranslational modifications. Besides ovaries, LHR immunoreactivity (LHRI) was also found in other tissues, such as the brain, fallopian tube, endometrium, trophoblast and resident tissue macrophages. The 3B5 mouse monoclonal antibody was raised against purified rat LHR. In rat, porcine and human ovaries, the 3B5 identified six distinct LHR bands migrating at approximately 92, 80, 68, 59, 52 and 48 kDa. Characteristic LHRI was detected in rat, human and porcine corpora lutea. During cellular differentiation, subcellular LHR distribution changed from none to granular cytoplasmic, perinuclear, surface, nuclear and no staining. …

Evidence Of An Interaction Between Nifedipine And Nafcillin In Humans, Zahurin Mohamed

Zahurin Mohamed

Aims Nafcillin (Wyeth Laboratories, Philadelphia, PA, USA) has been reported to induce the metabolism of cyclosporin and warfarin, which are known substrates of cytochrome P-450 (CYP). However, there has not been any report to date on its possible interaction with nifedipine, an index substrate of the enzyme, CYP3A4. Methods Nine healthy normotensive subjects participated in this randomized placebo-controlled two-way crossover study examining the effects of 5 days' pretreatment of nafcillin 500 mg or placebo four times daily on the pharmacokinetics of an oral dose of nifedipine 10 mg. Plasma nifedipine concentrations were measured by gas chromatography-mass spectro. Results The area …

Interaction Between Adrenergic And Nitric Oxide-Releasing Nerves In Cerebral Arteries, Emmanuel Ngu Mbaku

Loma Linda University Electronic Theses, Dissertations & Projects

Of all body tissues, the brain is the least tolerant of ischemia so much that interruption of cerebral blood flow by 5 sec can cause syncope, with irreversible tissue damage if the ischemia last a few minutes. In this respect, the body has an imperative duty of ensuring constant and adequate blood supply to the brain. It does so through a series of intrinsic and extrinsic control factors of which adrenergic and nitric oxide-releasing nerves are components. My research examined the interaction between these two nerve-types with emphasis on the mechanisms involved as well as on changes in their functions …

Cellular Proliferation, Death And Histology In Gambusia Affinis Livers After Exposure To 2-Aminofluorene And Benzidine, Susan J. Lentz

Dissertations

A fundamental need exists for aquatic animals in the biomonitoring of aquatic ecosystems for environmental carcinogens. G. affinis, a small fish species, is selected for this study due to its hardiness, non-migratory nature, geographic diversity and ability to develop tumors. Molecular tools for cellular proliferation and death are examined for use with G. affinis. Additionally, these tools are used to investigate the early carcinogenic effects of 2-aminofluorene and benzidine, both individually and combined, on G. affinis livers.

Antibodies to proliferating cell nuclear antigen (PCNA), p53, cleaved caspase-3 and cleaved poly(ADP-ribose) polymerase (PARP) were examined for use in G. …

Studies On The Regulation Of Mu Opioid Receptor Mrna Expression In Shsy-5y Human Neuroblastoma Cells, Xin Yu

Seton Hall University Dissertations and Theses (ETDs)

Prolonged exposure to morphine down-regulates mu opioid receptors (MOR) on both undifferentiated and differentiated (retinoic acid or phorbol ester treated) SHSY-5Y cells. However, morphine pretreatment does not alter MOR receptor affinity for morphine. To investigate the molecular basis for MOR regulation after exposure to its selective agonists, we have developed a quantitative competitive reverse transcriptase-polymerase chain reaction (QC-RT PCR) to quantify the expression of MOR in SHSY-SY cells. Differentiation of SHSY-5Y cells with retinoic acid or phorbol ester up regulated MOR mRNA expression by 30 % and 78%, respectively. A 24 hours treatment with morphine (10 µM) down regulated MOR …

The Effects Of Alternative-Site Blood Glucose Monitoring On Testing Frequency, Pain Rating, And Glycosylated Hemoglobin, Nancy Bennion

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A crossover design study was conducted to determine if reducing pain, by using alternative sites off the finger tip, would increase testing frequency and improve clinical outcome as measured by glycosylated hemoglobin. Subjects with type 1 and type 2 diabetes tested with the FreeStyle alternative-site meter (group 1) or tested with their original meter (group 2). After 3 months the subjects used the alternate meter. Testing frequency and blood glucose concentrations were recorded for the month before the study began and monthly thereafter. Glycosylated hemoglobin was tested initially, at the crossover point, and at study conclusion. Insulin users increased testing …

Stochastic Intra-Cellular Modeling, Thomas E. Hopkins

Theses and Dissertations

Air Force personnel may sometimes come into contact with potentially harmful chemicals while performing their duties. Of course the Air Force desires to keep any potential health risks to its members to a minimum. To this end the Air Force would like to identify which chemicals are toxic, their level of toxicity, and the processes by which these chemicals disrupt normal biological activities at the cellular level. The development of mathematical models can be of great benefit to toxicity studies. Because real world systems involve randomness, that is noise, and the desire is to create mathematical models to represent those …

Deterministic Intracellular Modeling, Jacqueline B. Young

Theses and Dissertations

The United States Air Force is interested in the potential side effects at the cellular level from exposure to mission-essential chemicals. Presently, Air Force toxicology studies are conducted to help shed light in identifying potential hazards to workers. However, it takes a considerable amount of money, resources, and time to obtain and analyze experimental results from toxicology studies. The necessity for innovative methods that enable researchers to more effectively generate and analyze data is apparent.

Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Estrogens play an important role in the regulation of placental function, and 17-beta-estradiol (E2) production rises eighty fold during human pregnancy. Although term placenta has been found to specifically bind estrogens, cellular localization of estrogen receptor alpha (ER-alpha) in trophoblast remains unclear. We used western blot analysis and immunohistochemistry with h-151 and ID5 monoclonal antibodies to determine the expression and cellular localization of ER-alpha protein in human placentae and cultured trophoblast cells. Western blot analysis revealed a ~65 kDa ER-alpha band in MCF-7 breast carcinoma cells (positive control). A similar band was detected in five normal term placentae exhibiting strong …

Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder

Maria Cekanova MS, RNDr, PhD

Estrogens play an important role in the regulation of placental function, and 17-beta-estradiol (E2) production rises eighty fold during human pregnancy. Although term placenta has been found to specifically bind estrogens, cellular localization of estrogen receptor alpha (ER-alpha) in trophoblast remains unclear. We used western blot analysis and immunohistochemistry with h-151 and ID5 monoclonal antibodies to determine the expression and cellular localization of ER-alpha protein in human placentae and cultured trophoblast cells. Western blot analysis revealed a ~65 kDa ER-alpha band in MCF-7 breast carcinoma cells (positive control). A similar band was detected in five normal term placentae exhibiting strong …

Manganese Distribution Across The Blood-Brain Barrier. I. Evidence For Carrier-Mediated Influx Of Managanese Citrate As Well As Manganese And Manganese Transferrin, Janelle S. Crossgrove, David D. Allen, Bonny L. Bukaveckas, Susan S. Rhineheimer, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Manganese (Mn) is an essential element and a neurotoxicant. Regulation of Mn movement across the blood–brain barrier (BBB) contributes to whether the brain Mn concentration is functional or toxic. In plasma, Mn associates with water, small molecular weight ligands and proteins. Mn speciation may influence the kinetics of its movement through the BBB. In the present work, the brain influx rates of ⁵⁴Mn²⁺, ⁵⁴Mn citrate and ⁵⁴Mn transferrin (⁵⁴Mn Tf) were determined using the in situ brain perfusion technique. The influx rates were compared to their predicted diffusion rates, which were determined from …

Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …

Overview Of Data And Conceptual Approaches For Derivation Of Quantitative Structure-Activity Relationships For Ecotoxicological Effects Of Organic Chemicals, Steven P. Bradbury, Christine L. Russom, Gerald T. Ankley, T. Wayne Schultz, John D. Walker

Steven P. Bradbury

The use of quantitative structure–activity relationships (QSARs) in assessing potential toxic effects of organic chemicals on aquatic organisms continues to evolve as computational efficiency and toxicological understanding advance. With the everincreasing production of new chemicals, and the need to optimize resources to assess thousands of existing chemicals in commerce, regulatory agencies have turned to QSARs as essential tools to help prioritize tiered risk assessments when empirical data are not available to evaluate toxicological effects. Progress in designing scientifically credible QSARs is intimately associated with the development of empirically derived databases of well-defined and quantified toxicity endpoints, which are based on …

Qsar Prioritization Of Chemical Inventories For Endocrine Disruptor Testing, Steven P. Bradbury, Patricia Schmeider, Ovanes Mekenyan, Gilman Veith

Steven P. Bradbury

Binding affinity between chemicals and the estrogen receptor (ER) serves as an indicator of the potential to cause endocrine disruption through this receptor-mediated endocrine pathway. Estimating ER-binding affinity is, therefore, one strategic approach to reducing the costs of screening chemicals for potential risks of endocrine disruption. While measuring ER binding with in vitro assays may be the first choice in prioritizing chemicals for additional in vitro or in vivo estrogenicity testing, the time and costs associated with screening thousands of chemicals is prohibitive. Recent advances in 3D modeling of the reactivity of flexible structures make in silico methods for estimating …

An Overview Of The Use Of Quantitative Structure-Activity Relationships For Ranking And Prioritzing Large Chemical Inventories For Environmental Risk Assessments, Steven P. Bradbury, Christine L. Russom, Roger L. Breton, John D. Walker

Steven P. Bradbury

Ecological risk assessments for chemical stressors are used to establish linkages between likely exposure concentrations and adverse effects to ecological receptors. At times, it is useful to conduct screening risk assessments to assist in prioritizing or ranking chemicals on the basis of potential hazard and exposure assessment parameters. Ranking of large chemical inventories can provide evidence for focusing research and/or cleanup efforts on specific chemicals of concern. Because of financial and time constraints, data gaps exist, and the risk assessor is left with decisions on which models to use to estimate the parameter of concern. In this review, several methods …

Influence Of Suspended Solids On Acute Toxicity Of Carbofuran To Daphnia Magna: Ii. An Evaluation Of Potential Interactive Mechanisms, Steven P. Bradbury, Carl Herbrandson, Deborah L. Swackhamer

Steven P. Bradbury

It has been demonstrated that simultaneous exposure of Daphnia magna to suspended solids and a carbamate pesticide potentiates the toxic response to the pesticide. The toxicodynamics between these stressors were investigated to determine possible mechanisms of interaction. Three experimental series were conducted with D. magna to determine: the effect of food availability on carbofuran toxicity; the effect of food availability on jointly administered carbofuran and suspended solids; and changes in the magnitude of effects which can occur with suspended solids of different composition. These experiments demonstrated that both carbofuran toxicity and the joint toxicity of carbofuran and suspended solids to …

Quantitative Structure-Activity Relationship Models For Prediction Of Estrogen Receptor Binding Affinity Of Structurally Diverse Chemicals, Steven P. Bradbury, Patricia K. Schmeider, Gerald Ankley, Ovanes Mekenyan, John D. Walker

Steven P. Bradbury

The demonstrated ability of a variety of structurally diverse chemicals to bind to the estrogen receptor has raised the concern that chemicals in the environment may be causing adverse effects through interference with nuclear receptor pathways. Many structure–activity relationship models have been developed to predict chemical binding to the estrogen receptor as an indication of potential estrogenicity. Models based on either two-dimensional or three-dimensional molecular descriptions that have been used to predict potential for binding to the estrogen receptor are the subject of the current review. The utility of such approaches to predict binding potential of diverse chemical structures in …

Influence Of Suspended Solids On Acute Toxicity Of Carbofuran To Daphnia Magna: I. Interactive Effects, Steven P. Bradbury, Carl Herbrandson, Deborah L. Swackhamer

Steven P. Bradbury

This study explored the effects on Daphnia magna from exposure to the pesticide carbofuran in combination with stress from suspended solids exposure. Our objective was to assess whether suspended solids affects the toxicodynamic response of D. magna to carbofuran. A series of laboratory experiments was performed where animals were exposed to carbofuran concentrations ranging from 0 to 160 mg/l in combination with suspended solids concentrations ranging from 0 to 10,000 mg/l. In the absence of suspended solids, effects of carbofuran were dose dependent and resulted in an EC(sub)50 of 92 mg/l. Exposure to suspended solids, up to extreme levels that …

Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder

Maria Cekanova MS, RNDr, PhD

During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …

Joint Effects Of Population Density And Toxicant Exposure On Population Dynamics Of Capitella Sp. I, Valery E. Forbes, Richard M. Sibly, Inez Linke-Gamenick

Valery Forbes Publications

Very few studies have analyzed the dependence of population growth rate on population density, and even fewer have considered interaction effects of density and other stresses, such as exposure to toxic chemicals. Yet without such studies we cannot know whether chemicals harmful at low density have effects on carrying capacity or, conversely, whether chemicals reducing carrying capacity are also harmful at low density, impeding a population’s capacity to recover from disturbance. This study examines the combined effects of population density and a toxicant (fluoranthene) on population growth rate (pgr) and carrying capacity using the deposit-feeding polychaete Capitella sp. I as …

Analysis Of Phycotoxin And Mycotoxin, Contaminants In Foods, Using Liquid Chromatography - Mass Spectrometry Techniques, Ana Brana Magdalena

Theses

The impact of phycotoxins and mycotoxins on human and animal health are of important concern for worldwide authorities and the scientific community. Liquid chromatography-mass spectrometry (LC-MS) techniques have been developed, optimised and validated for the analysis of these toxins.

Phycotoxins are potentially toxic substances, which are produced by marine phytoplankton and which may accumulate in shellfish or finfish. Azaspiracid poisoning (AZP) is a recently discovered syndrome that has been implicated in several incidents of human intoxications. A study was undertaken to examine the distribution of azaspiracids (AZA) in scallops {Pecten maximiis) and mussels {Mytiliis edulis). Domoic acid (DA) is an …

The Two Isoforms Of Rat Metallothionein Are Coordinately Regulated In Vivo, Diane M. Todd

Browse all Theses and Dissertations

Metallothionein (MT) is an inducible protein whose unique structure contributes to its many functions. One of MT’s functions is the detoxification of heavy metals. Cadmium (Cd), which is an environmental pollutant and a hazard to both humans and animals, is detoxified by MT. There are four known MT isoforms (MT I-IV) and this study focuses on only MT I and MT II. Even though a lot is known about the effect of Cd on MT induction little has been reported about the MT isoforms and their pattern of mRNA expression. It is the aim of this study to investigate the …

Pharmaceutical Care Scales And Thai Hospital Pharmacists’ Attitude Toward Pharmaceutical Care(แบบวัดการบริบาลเภสัชกรรมและทัศนคติต่องานบริบาลเภสัชก..., Ratanaporn Awiphan, Unchalee Thadapark

The Thai Journal of Pharmaceutical Sciences

Pharmaceutical care is the concept expanding pharmacists’ role in health care, which urges their increasing responsibility in collaboration with other health professionals in taking care of patients. The objectives of this study were to l) develop the ...

Screening Of Some Rubiaceous Plants For Cytotoxic Activity Against Cervix Carcinoma (Kb-3-1) Cell Line (การคัดกรองความป็นพิษต่อเซลล์มะเร็งปากมดลูกชนิด Kb-3-1 ข..., Patcharawan Tanamatayarat, Porn-Ngarm Limtrakul, Sunee Chunsakaow, Chadarat Duangrat

The Thai Journal of Pharmaceutical Sciences

Twenty plants in the family Rubiaceae collected from Northern Thailand were extracted and compared for their cytotoxic activities against a human cervix carcinoma cell line (KB-3T). MTT assay was the technique utilized for cell survival determination. Th...

Chemical Constituents Of Cleidion Spiciflorum Leaves(องค์ประกอบทางพฤกษเคมีของใบดีหมี), Supotchana Menkham, Rutt Suttisri

The Thai Journal of Pharmaceutical Sciences

Two flavone glycosides, acacetin-7-(VP-D-glucopyranoside and diosmetin-7-O-P-D glucopyranoside, a phenylpropanoid glycoside, p-propenylphenoipD -glucopyranoside, a linear diterpene, Érans-phytol, and a lanostane triterpene, (24$-24-methyl-5a-lanosta-9(1...

A High Pressure Liquid Chromatographic Method For The Determination Of Arecoline In Areca Nuts(การหาปริมาณอะรีโคลีนในผลหมากโดยวิธีโครมาโทกราฟีสมรรถน..., Chantana Aromdee, Sirinart Panuwongse, Rutchayaporn Anorach, Suwanna Vorarat

The Thai Journal of Pharmaceutical Sciences

A reversed phase high pressure liquid chromatography was developed to determine arecoline in areca nut. The column was 018, the mobile phase was a phosphate buffer pH 5.5, and the UVdetector was set at 216 nm. The method was used to determine the content...

Assessment Of Outpatient Knowledge, Attitudes, And Satisfaction With Service In Diabetic Clinic At King Chulalongkorn Memorial Hospital(การประเมินความรู้ทัศนคติและความพึงพอใจต..., Supakit Wongwiwatthananukit, Rathaporn Lohavisavapanich

The Thai Journal of Pharmaceutical Sciences

The purpose of this cross-sectional survey study was to assess outpatient knowledge, attitudes, and satisfaction with service in diabetic clinic at King Chulalongkorn Memorial Hospital. The study was conducted between May 9 and June 10, 2003. A sample of...

Antimutagenicity On Urethane Of Various Soybean Products Using In Vivo Somatic Mutation And Recombination Test(การต้านฤทธิ์ก่อกลายพันธุ์ของยูรีเทนโดยผลิตภัณฑ์..., Kaew Kangsadalampai, Piyanart Sommani

The Thai Journal of Pharmaceutical Sciences

Different soy bean products, namely fermented products (soy paste, red sufu, yellow sufu and Tooa Nao and nonfermented products (Textured Vegetable Protein or TVP and dried-soybean curd or yuba), were evaluated for their antimutagenicity against urethane...

Determination Of Flavonoids In Kaempferia Parviflora By Gas Chromatographie Method(การวิเคราะห์หาปริมาณฟลาโวนอยด์ในกระชายดำโดยวิธีแก็ส โครมา..., Supawadee Daodee, Chavi Yenjai, Cattareya Suttanut, Suganya Supattanapong

The Thai Journal of Pharmaceutical Sciences

Kaempferia parviflora is a herbal plant which is commercially popular at the present time. These products are available in several dosage forms, e.g. capsule and powder for decoction. This study aimed to determine the constituents in 17 samples of Kaempf...