Life Sciences Commons^™

Erecta Family Genes Regulate The Shoot Apical Meristem And Organ Formation, Daniel A. Degennaro

Doctoral Dissertations

Plants are sessile and must adjust their organ growth to their environments. A reservoir of stem cells in the shoot apical meristem (SAM) supplies cells for differentiation into organs. The SAM must balance organ production with stem cell maintenance. The ERECTA family (ERfs) encodes the leucine-rich repeat receptor-like kinases ERECTA (ER), ERECTA-LIKE 1 (ERL1), and ERL2. ERf signaling regulates organ initiation and stem cell maintenance. Results presented in this work include the following:

1) WUSCHEL (WUS) and CLAVATA3 (CLV3) make up a negative feedback loop to maintain SAM size. WUS and CLV3 expression localization is critical for …

Root Stage Distributions And Their Importance In Plant-Soil Feedback Models, Tyler Poppenwimer

Doctoral Dissertations

Roots are fundamental to PSFs, being a key mediator of these feedbacks by interacting with and affecting the soil environment and soil microbial communities. However, most PSF models aggregate roots into a homogeneous component or only implicitly simulate roots via functions. Roots are not homogeneous and root traits (nutrient and water uptake, turnover rate, respiration rate, mycorrhizal colonization, etc.) vary with age, branch order, and diameter. Trait differences among a plant’s roots lead to variation in root function and roots can be disaggregated according to their function. The impact on plant growth and resource cycling of changes in the distribution …

Needles In A Haystack Of Protein Diversity: Interrogation Of Complex Biological Samples Through Specialized Strategies In Bottom-Up Proteomics Uncover Peptides Of Interest For Diverse Applications, Manuel I. Villalobos Solis

Doctoral Dissertations

Peptide identification is at the core of bottom-up proteomics measurements. However, even with state-of the-art mass spectrometric instrumentation, peptide level information is still lost or missing in these types of experiments. Reasons behind missing peptide identifications in bottom-up proteomics include variable peptide ionization efficiencies, ion suppression effects, as well as the occurrence of chimeric spectra that can lower the efficacy of database search strategies. Peptides derived from naturally abundant proteins in a biological system also have better chances of being identified in comparison to the ones produced from less abundant proteins, at least in regular discovery-based proteomics experiments. This dissertation …

Characterization Of An Ethylene Receptor In Synechocystis Sp. Pcc 6803, Randy Francis Lacey

Doctoral Dissertations

In plants, ethylene functions as a hormone regulating many growth and developmental processes. Ethylene receptors in plants resemble bacterial two-component signaling systems. Because of this it, ethylene receptors are thought to have been acquired by gene transfer from the cyanobacterial endosymbiont that lead to the development of the chloroplast. However, prior to this work, functional ethylene receptors were thought to only be found in green plants. Here, we show that the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis) contains a functional ethylene receptor, SynEtr1. SynEtr1 contains a predicted ethylene binding domain, a photosensory cyanobacteriochrome (CBCR) domain, and a histidine …

Impacts Of Biofumigation And Anaerobic Soil Disinfestation On Strawberry Production, Jennifer Renee’ Wheeler

Masters Theses

Due to the phase-out of methyl bromide, there is a need for alternative, nonchemical fumigation treatments in strawberry production. Anaerobic soil disinfestation and biofumigation are two non-chemical methods for controlling soilborne plant pathogens of strawberry. This study was designed to observe strawberry fruit nutrition and soil volatiles of a strawberry field being treated with biofumigation treatments, anaerobic soil disinfestation treatments, and a combination of the two alternative methods. A trial was conducted with 11 pre-plant soil-incorporated treatments arranged in a randomized complete block design with 6 rows (blocks). Biofumigation treatments consisted of deactivated mustard meal, deoiled mustard meal, mustard pellets, …

Sources Of Heat In Synthetic Turf Systems, Adam William Thoms

Doctoral Dissertations

With options for cooling synthetic turf limited, an outdoor experiment was conducted at the University of Tennessee Center for Athletic Field Safety (Knoxville, TN) to develop a model to predict maximum, minimum, and mean synthetic turf surface temperature using forecasted atmospheric conditions. Synthetic turf surface temperature varied due to both air temperature and solar radiation. Predictive models using these data accounted for 86, 95, and 94% of the variation in daily maximum, minimum, and mean synthetic turf surface temperature. Accuracy of these models for predicting daily mean and minimum synthetic turf surface temperature using 48 and 72-hour forecasted air temperature …

Map Supplements For The Metallic Woodboring Beetles (Coleoptera: Buprestidae) Of Tennessee, William E. Klingeman Iii, Jason A. Hansen, Joshua P. Basham, Jason B. Oliver, Nadeer N. Youssef, Whitney Swink, Christine A. Nalepa, Donna C. Fare, J. Kevin Moulton

Plant Sciences Publications and Other Works

Following compilation of distribution records and seasonal flight activity information for 106 metallic wood-boring beetle (Coleoptera: Buprestidae) species for Tennessee, maps were updated to provide county-level collection notations for 10,543 published records and specimen collections made from 1934 to 2013. County collection tallies across the ecoregions in Tennessee are also presented. Maps for individual species highlight gaps in key geographic areas where specimens have not been collected and can be valuable for future biosurveillance, monitoring and management efforts for these economically and ecologically important insects.

Development And Evaluation Of A Cost Effective Plant Growth Media Moisture Sensor And Development Of An Aqueous Data Transmission System For Irrigation Purposes, Steven Michael Pickett

Masters Theses

The ability to accurately monitor and transit the moisture content of soilless growing media in the rooting zone is critical for plant-based research, production of high value crops, and other agricultural production. The focus of this study is the development and evaluation of a cost effective moisture sensor designed to measure the plant-available moisture content of growing media and the development of a aqueous data transmission method for relaying this information back to a central location. While there are currently many commercially available soil moisture sensors on the market, the aim of this research is not to develop a more …

Evaluating Pollination Ecology Of The Endangered Pityopsis Ruthii (Small) Small (Asteraceae), Philip Anthony Moore

Masters Theses

Pityopsis ruthii (Small) Small, also known as Ruth’s golden aster, is a federally endangered herbaceous perennial, endemic to two river systems, the Hiwassee and the Ocoee, within the Cherokee National Forest, Polk County, Tennessee. There are approximately 13,000 individuals that may be at high risk of short-term extirpation (Thompson and Schwartz, 2006). Little is known of the basic reproduction and life history of P. ruthii. Clebesh and Sloan (1993), Cruzan (2001), Park (1998), and Wadl et al. (2014) found evidence that seed production and seed viability are highly variable. Clebesh and Sloan (1993) indicated that pollinator visitation was highly temporal …

Evaluation Of Crumb Rubber Dynamics For Improving Athletic Field Quality, Kyley Hampton Dickson

Masters Theses

Crumb rubber (CR) is an amendment used to reduce surface hardness and increase wear-tolerance on athletic fields. Turf managers can topdress CR particles into highly trafficked portions of athletic fields; however, optimum particle size and topdressing depth combinations for use on bermudagrass (Cynodon spp) athletic fields have not been determined. Optimum CR particle size and depth to maximize performance of hybrid bermudagrass (C. dactylon (L.) Pers. x C. transvaalensis Burtt-Davy, 'Tifway') athletic field turf established on a Sequatchie silt loam soil was investigated at the University of Tennessee Center for Athletic Field Safety (Knoxville, TN) in 2011 and …

Do Plants Play A Part In Student Satisfaction?, Amanda Diane Plante

Masters Theses

Environmental psychologists have found relationships between plants, nature and satisfaction. Student satisfaction is important across grade levels. Two studies were conducted to determine the effect of spending time with live plants on student satisfaction and academic performance. In the first study, a quasi-experimental nonequivalent control group design was used to determine how participation in garden labs would affect high school student satisfaction with school and academic performance. Ecology students in the variable group participated in ten gardening labs during the semester. During labs, students did hands-on gardening activities in the school greenhouse and garden. Students in the variable and control …

Synthetic Turf Surface Temperature Reduction And Performance Characteristics As Affected By Calcined Clay Modified Infill, Eric Hall Reasor

Masters Theses

Synthetic turf research plots containing crumb rubber (CR) infill were established in Knoxville, TN in 2012 and 2013. Calcined clay (CC) was amended to CR in several ratios: a 50:50 (vol vol-1) blend; a 50:50 blend with a polymer coating on CC (50 CR:50 CCC); a 15 mm layer of CC under 15 mm of CR (CR over CC); and a 15 mm layer of CC over 15 mm of CR (CC over CR). A 100% CR and a 70:30 blend of CR to sand (70 CR:30 S) were included for comparison. Surface temperature was measured in the summer of …

Forage And Biomass Dual-Purpose Harvest System Using Native Warm-Season Grasses, David Weston Mcintosh

Masters Theses

There has been increasing interest in utilizing native warm-season grasses (NWSGs), especially switchgrass, as a biomass feedstock for cellulosic ethanol production. Millions of hectares of crop and pasture in the mid-South are forecast to potentially be planted with switchgrass for biomass feedstock production. This could have a substantial impact on the region’s cattle industry, reducing forage production hectares. This study was conducted to determine the effect of early season harvest timing on forage and biomass of NWSGs designed for use in cellulosic ethanol production. The over-all hypothesis was to determine if an early forage harvest can be included in a …

Soybean Nodulin 26: A Channel For Water And Ammonia At The Symbiotic Interface Of Legumes And Nitrogen-Fixing Rhizobia Bacteria, Jin Ha Hwang

Doctoral Dissertations

During the infection and nodulation of legume roots by soil bacteria of the Rhizobiaceae family, the invading endosymbiont becomes enclosed within a specialized nitrogen-fixing organelle known as the "symbiosome". In mature nodules the host infected cells are occupied by thousands of symbiosomes, which constitute the major organelle within this specialized cell type. The symbiosome membrane is the outer boundary of this organelle which controls the transport of metabolites between the symbiont and the plant host. These transport activities include the efflux of the primary metabolic product of nitrogen fixation and the uptake of dicarboxylates as an energy source to support …

Alternative Poa Species As Permanent And Temporary Turfgrasses In The Transition Zone, Samuel Lucas Freshour

Masters Theses

As restrictions on water, fertilizer, and pesticide use increase, along with public pressure, turfgrass practitioners will need ways to meet the challenges of reduced inputs while still maintaining acceptable turf quality. Proper selection of turfgrass species and cultivars that are adapted to the climate, intended use, and level of expectation is the best and first line of defense for reduced input situations. The objective of this study was to evaluate alternative species from the bluegrass (Poa) genus for their performance as both permanent and temporary winter turfgrass species. In 2010 and 2011, 45 different treatments consisting of 37 different bluegrasses …

Comparison Of Roundup Ready And Conventional Soybean (Glycine Max L.) Weed Control Systems For Optimizing Yield And Economic Profitability, Brittany Lee Gaban

Masters Theses

Research was conducted in 2010, 2011 and 2012 at the East Tennessee Research and Education Center in Knoxville, TN, in order to compare differences in soybean yield among differing levels of weed control within Roundup Ready® [Glyphosate-resistant] (RR)and conventional soybean cultivars to gain a better understanding of the impact different intensities of weed control have on RR and conventional cropping systems. Results determined that after applying the weed control regimens, there was no significant difference (p

Glyphosate resistant weeds introduce new challenges and create a more costly weed control regimen, especially when using a RR based soybean cultivation operation. Therefore, …

Impacts Of Preemergence Herbicides On Hybrid Bermudagrass Morphology, Patrick Alan Jones

Masters Theses

Preemergence (PRE) herbicides were evaluated in multiple experiments to determine their impacts on hybrid bermudagrass morphology [(C. dactolyn (L.) Pers. x C. transvaalensis (Burtt Davy)]. First, indaziflam and prodiamine were applied to hybrid bermudagrass rooted to various depths in either sand or silt loam mini-rhizotrons. Results indicated that prodiamine (a mitiotic inhibitor) and indaziflam (a cellulose biosynthesis inhibitor) both significantly reduced root length density compared to a non-treated control; however, indaziflam treated plants exhibited foliar injury (i.e., reddening, necrosis) while those treated with prodiamine did not.

The researchers surmised that reductions in root length density with indaziflam may compromise …

Consequences Of Gene Flow And Transgene Introgression In Hybrids Between Transgenic Brassica Napus And Its Weedy Wild Relative Brassica Rapa, Reginald Jason Millwood

Masters Theses

The adventitious presence of transgenes and their potential impact on the environment has been a topic of concern for many years. To address these concerns the following chapters discuss past and current research of gene flow and introgression, methods for transgene detection and monitoring, and the results from field-level experiments using artificially introgressed advanced generation hybrids. The field studies were designed to be a worst-case scenario where hybrids were produced by hand-crossing transgenic Brassica napus (AACC, 2n = 38) and its weedy wild relative Brassica rapa (AA, 2n = 20). B. napus was transgenic for the green fluorescent protein [m-GFP-5 …

Establishment And Persistence Of Legumes In Switchgrass Biomass And Forage/Biomass Production Systems, Kara Spivey Warwick

Masters Theses

Switchgrass, Panicum virgatum, is being developed as an economically and ecologically sustainable biomass crop. Nitrogen is considered one of the most limiting inputs of switchgrass. Alternatives to synthetic nitrogen fertilization may be nitrogen-fixing legumes interseeded into switchgrass. The objectives of this research were: (1) develop efficient legume management strategies for switchgrass production systems, (2) evaluate and identify cool and warm-season legumes that can be grown compatibly with switchgrass, (3) determine whether switchgrass yields are increased by legume N-fixation, and (4) determine N-fixation of common (Vicia sativa) and hairy vetch (Vicia villosa).

This study examined the …

Effects Of Trinexapac-Ethyl And Winter Overseeding On The Morphological Characteristics And Traffic Tolerance Of Bermudagrass Cultivars, William D. Haselbauer

Masters Theses

Common bermudagrass [Cynodon dactylon (L.) Pers.] and hybrid bermudagrass [C. dactylon (L.) Pers. X C. transvaalensis Burtt-Davy] are some of the most common turfgrasses used on athletic fields. Subsequently, the traffic tolerance of commercially available cultivars and the effects of trinexapac-ethyl (TE) on bermudagrass athletic fields with or without perennial ryegrass are often limited. A two year study at the University of Tennessee was conducted under simulated athletic field traffic to evaluate the performance of bermudagrass cultivars as affected by TE and overseeding. Bermudagrass cultivars evaluated were Tifway, Riviera, Patriot, and Celebration. TE treatments consisted of an untreated …

Factors Influencing Desire For Increased Wildlife Habitat Among Tennessee Farmers And The Economics Of Switchgrass Production, Janet Jones

Masters Theses

In order to keep up with a growing human population, wildlife habitat has had to be relinquished. Modern technology has furthered the abilities of commodity producers but caused a deterioration of the quality and quantity of habitat available for wild animals in many cases. Many species of wildlife have left areas of the state in order to meet their basic needs. In order to increase wildlife numbers, wildlife habitat will have to be reintroduced or managed differently. The first objective of this research is to identify and evaluate the factors associated with a demand for increased wildlife habitat among Tennessee …