Life Sciences Commons^™

Assessing The Impact Of The Tennessee Equine Industry, Olivia Watson

Masters Theses

The Tennessee equine industry is alive and prevalent across the entire state. However, due to the expansiveness of the industry, lack of consistent record keeping, and large discrepancies among reporting agencies, it has proven to be difficult to accurately account for the total impact of the industry in terms of population, demographics, and overall economic impact. The objective of this study was to 1) determine population demographics and economic impact of the equine industry in Tennessee and 2) determine the public perception on the addition of live equine racing within the state. An anonymous, online assessment was developed (March-August 2022; …

Evaluation Of Treatment Options And Investigation Of Pathogenesis Of Chytridiomycosis In North American Salamanders, Anastasia Elaine Towe

Doctoral Dissertations

Chytridiomycosis, a cutaneous fungal disease caused by two related fungi, Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bsal), is a major cause of amphibian population declines worldwide. Bd has a broad distribution, covering every continent except for Antarctica. Meanwhile, Bsal is currently restricted to Europe and Asia. Treatment options for both Bd and Bsal remain a major area of concern for wild populations. Recently, implants for long-term, parenteral antifungal drug administration have shown some promise in fungal diseases in wildlife. We investigated the safety and efficacy of an intracoelomic implant of the antifungal drug terbinafine in greater …

Microplastics Exposure In At-Risk Myotis Lucifugus Bats Of The Northeastern United States, Leah Crowley

Chancellor’s Honors Program Projects

No abstract provided.

Locked Nucleic Acid Aptamer And 10 Nm Gold Nanoparticles Increases The Sensitivity Of A Prion Protein Detection Assay, Haley Channell

Chancellor’s Honors Program Projects

No abstract provided.

Effects Of Protein Concentration And Beta-Adrenergic Agonists On Ruminal Microbial Communities In Finishing Beef Heifers, Alison P. Pfau

Masters Theses

To improve animal performance and modify growth by increasing lean tissue accretion, beef cattle production has relied on use of growth promoting technologies such as beta-adrenergic agonists. These synthetic catecholamines, combined with the variable inclusion of rumen degradable (RDP) and undegradable protein (RUP), may improve feed efficiency and rate of gain in finishing beef cattle. However, research about the impact of beta-adrenergic agonists and protein level and source on the ruminal microbiome is limited. The objective of this study was to determine the effect of different protein concentrations and beta-adrenergic agonist (ractopamine hydrochloride; RAC usage) on ruminal bacterial communities in …

Bat Population Status And Roost Selection Of Tri-Colored Bats In The Great Smoky Mountains National Park In The Era Of White-Nose Syndrome, Grace Marie Carpenter

Masters Theses

The ongoing spread of white-nose syndrome is causing devastating declines range-wide for certain North American bat species. Baseline population data that would help mangers monitor bat populations in the face of WNS is lacking. Likewise, knowledge of summer roosts, a limiting resource for tri-colored bats (Perimyotis subflavus), a species threatened by WNS, is lacking in the southern portion of their range. In our study, we investigated the effect that WNS has had on a population of tricolored bats in the Great Smoky Mountains National Park, TN-NC. We also characterized summer roosts for the species at the microhabitat and …

Surveillance Of Ticks Parasitizing Tennessee Beef Cattle And Investigations Into The Microbial Communities Of Cattle Associated And Questing Amblyomma Maculatum, David Paul Theuret

Masters Theses

Despite the risks that ticks and tick-borne disease pose to the beef cattle industry, many Tennessee producers are unaware of the dangers they represent. This mindset could facilitate the invasion and establishment of exotic ticks and pathogens that would devastate the cattle industry. Current control practices rely on chemical methods, which are not effective long-term; therefore, investigations into creating an integrated approach to control would create more sustainable methods. This study aims to address this through two objectives: The first is to determine the species composition, seasonal prevalence, geographic distribution and diversity of ticks on Tennessee cattle. The second is …

Utilizing Pregnancy Associated Glycoproteins To Improve Reproductive Efficiency In Cattle, Sydney Taylor Reese

Masters Theses

Diagnosing and maintaining successful pregnancies are one of the most important components to profitable and efficient management of beef and dairy cattle operations. Pregnancy loss is a major component of reproductive inefficiency and has been investigated less intensively in beef cattle than in dairy cattle. Pregnancy Associate Glycoproteins [PAGs] are placental products which have been identified as an accurate tool for pregnancy diagnosis and as substantial evidence indicates, markers of embryo and placental competence. The aim of these two studies is to further distinguish characteristics of pregnancies based on PAG concentration. Serial embryo transfer was used in beef heifers to …

Effect Of Varying Rumen Degradable And Undegradable Protein On Milk Production And Nitrogen Efficiency In Lactating Dairy Cows Under Summer Conditions, Jeffrey D. Kaufman

Masters Theses

The objective is to determine the effect of reducing nitrogen input through feeding low rumen degradable protein (RDP) and rumen undegradable protein (RUP) proportions on milk production, nitrogen efficiency and metabolism in heat-stressed cows. Forty-eight mid-lactating, Holstein cows were assigned to treatments using a randomized block design in a 2x2 factorial arrangement of treatments (n = 12/treatment). Treatments included two levels of RDP (10 and 8%) and two levels of RUP (8 and 6%). From d 1 to 21, a common diet (10% RDP-8% RUP) was fed to cows followed with their respective treatment diets fed from d 22 to …

Effect Of Omega-3 Polyunsaturated Fatty Acid (N-3 Pufa) Supplementation To Lactating Sows On Growth And Indicators Of Stress In The Post-Weaned Pig, John M. Mcafee

Masters Theses

The first experiment was designed to determine the concentration of protected fish oil product (PFO), as Gromega^TM, to be added that would sufficiently decrease the polyunsaturated fatty acid (PUFA) ratio in the sows’ milk and colostrum. Of the 3 diets tested (0.25%, 0.5% and 1%) with a control (0% PFO), only the 1% PFO diet had an effect on the PUFA concentrations. The docosahexaenoic acid (DHA) concentration tended (P = 0.05) to be greater in the 1% PFO diet for both colostrum and milk samples. A second experiment was designed to …

Follicular Dynamics In Insulin Resistant Mares, Julio Cesar Prado

Masters Theses

Obesity and insulin resistance have been linked to prolonged interovulatory period, aberrations in the estrous cycle, and continuous reproductive activity during the non-breeding season. EMS has been determined to influence the intrafollicular environment of mare ovaries. In humans, insulin resistance has been linked to polycystic ovaries as part of Polycystic Ovarian Syndrome (PCOS). A study was conducted to determine 1) the impact of insulin resistance on follicle growth and size at ovulation, and 2) whether predicted ovulatory follicles respond to hCG administration in Insulin-resistant (IR) mares. Mares were selected for the study based on insulin sensitivity and separated into an …

Association Of Genotype At Drd2 With Growth And Reproductive Performance Traits In Bulls Grazing Toxic Endophyte-Infected Tall Fescue, Kaysie Jennings

Masters Theses

Fescue toxicosis can negatively impact growth and fertility of beef cattle grazing tall fescue infected with an endophytic fungus. The fungus produces ergot alkaloids that contribute to the hardiness of the plant, but can be detrimental to the animal if consumed. A single nucleotide polymorphism (SNP) in the dopamine receptor D2 gene (DRD2) has been associated with serum prolactin concentrations and hair coat scores in cattle grazing toxic endophyte-infected (TE) tall fescue; cattle with the GG genotype had decreased serum prolactin concentrations and increased hair coat scores. Separately, heifers with the AA genotype calved sooner than AG or GG heifers. …

Relationships Of Temperament, Endocrine, Reproductive, And Behavioral Parameters Measured During Performance Testing Of Bulls, Sierra Ashley Lockwood

Masters Theses

The aim of this study was to examine relationships between bull temperament, behavior, growth, endocrine, and reproductive parameters measured over an 84 d test period. Bulls (n = 65) were reared in 6 pens separated on BW and age. Pen scores were assigned on d -1, 27, 55 and 83. On d 0, 28, 56, and 84, exit velocity, BW, time it took each bull to leave the chute, bull order through chute, and hair and blood samples were recorded or collected. Frame score was calculated, carcass ultrasounds and breeding soundness exams were performed on d 84. ADG was calculated …

The Spread And Potential Control Of Disease Across The Domestic Cattle-Wildlife Interface, Rachel Jackson

Chancellor’s Honors Program Projects

No abstract provided.

Health Monitoring Of Seasoned Sows In A New And Unfamiliar Environment, Rebecca Kocak

Chancellor’s Honors Program Projects

No abstract provided.

The Health Monitoring Of Naïve, Immunocompromised Sows In A Seasoned Facility, Sara Nash

Chancellor’s Honors Program Projects

No abstract provided.

Single-Nucleotide Polymorphisms Associated With Performance Traits In Beef Cattle Grazing Endophyte-Infected Tall Fescue, Bryan Christopher Bastin

Masters Theses

Tall fescue (Lolium arundinaceum Schreb.) is the most prevalent forage in the Midsouth United States due in part to the presence of the endophytic fungus Neotyphodium coenophialum. The fungus, while conferring hardiness to tall fescue, contributes to decreased production efficiency in cow-calf operations. A previous genome-wide association study was performed using the Illumina 50k bovine SNP chip. Twenty-four SNP were found to be associated (P < 0.05) with adjusted birth weight and adjusted 205-day weights of calves from 48 beef cows at Ames Plantation. The first objective was to validate each SNP by testing associations with several additional phenotypes. Custom Taqman genotyping assays (Applied Biosystems, Foster City, CA) were subsequently designed to genotype each SNP in beef cattle located at Tennessee Tech University (n = 654), to validate associations in a large, independent herd. The results yielded 15 associations that were significant (P < 0.05) with 6 phenotypes linked to those affected by fescue toxicosis. The second objective investigated the link between fescue toxicosis and the XK, Kell blood group complex subunit-related, member 4 …

Preservation Of Nutrients In Cool- And Warm-Season Forages At Different Stages Of Maturity And Management, Jason Allen Shultz

Masters Theses

The overall goals of the studies described in this thesis were to investigate management practices of tall fescue and native warm-season grasses (NWSG) and find the best time to harvest and method to preserve forage quality. Study one investigated the effects of maturity on tall fescue and switchgrass and the effects of preservation method on forage quality. This study confirmed that maturity reduced forage quality in both tall fescue and switchgrass. Both tall fescue and switchgrass were successfully preserved as haylage or hay and did not differ in forage quality. Forages harvested before mid-May met the TDN and CP requirements …

Pb1624 Managing Nuisance Animals And Associated Damage Around The Home, Craig A. Harper, Aubrey L. Deck

Wildlife and Fisheries

No abstract provided.

Northern Bobwhite (Colinus Virginianus) Population Ecology On Reclaimed Mined Lands, Evan Philip Tanner

Masters Theses

The northern bobwhite (Colinus virginianus) has experienced range-wide population declines for the past half century. The primary cause has been large-scale habitat loss and fragmentation. Through auspices of the Surface Mining Control and Reclamation Act of 1977 (SMCRA), large tracts of early successional vegetation have been created throughout much of the bobwhite’s range that may be managed to increase usable space. Peabody WMA is a reclaimed coal mine in Western Kentucky where bobwhite have been present in the past. To better understand the dynamics of this population and how habitat on Peabody WMA influences these dynamics, my two …

Evaluating The Relationship Between Ultrasound-Derived Carcass Characteristics And Production Traits In Angus Cattle, William Michael Mayfield

Masters Theses

Profitability in the beef industry is a crucial aspect of management schemes. The overall aim of this study was to determine if carcass ultrasonography in replacement heifers could explain variation in traits such as reproduction, a major factor in beef cattle efficiency and profitability. During a 10-year period, data were obtained from 906 yearling heifers through the use of carcass ultrasonography. The measurements collected included adjusted values for ribeye area (REA), percent intramuscular fat (%IMF), rib fat (RF), and rump fat. A retrospective analysis was performed on data collected and variables were separated into the extreme high and low 25% …

Evaluation Of The Effects Of The Closure Of Equine Slaughter Facilities, Taylor R. Bonar

Chancellor’s Honors Program Projects

No abstract provided.

Economic And Environmental Effects Of Switchgrass Production On A Representative Cow-Calf Farm In Middle Tennessee, Hanna Leah Watts

Masters Theses

The purpose of this research was to evaluate the economic tradeoffs and land use dynamics of incorporating switchgrass into the farm plan of a representative middle Tennessee cow-calf operation. A net revenue simulation and multi-year mathematical programming model was used to find the optimum mix of switchgrass and cow-calf enterprises that will maximize whole farm net revenues over 30 years on a representative middle Tennessee beef farm under both average and above average management. Land use changes predicted by the mathematical programming model were used to assess the carbon change associated with incorporating, or transitioning to, switchgrass production.

For a …

Capture-Recapture Of White-Tailed Deer Using Dna Sampling From Fecal Pellet-Groups, Matthew James Goode

Masters Theses

Reliable density estimates of game and keystone species such as white-tailed deer (Odocoileus virginianus) are desirable to set proper management strategies and for evaluating those strategies over time. However, traditional methods for estimating white-tailed deer density have been inhibited by behavior, densely forested areas that can hamper observation (detection), and invalid techniques of estimating effective trapping area. We wanted to evaluate a noninvasive method of mark-recapture estimation using DNA extracted from fecal pellets as the individual marker and for gender determination, coupled with a spatial detection function to estimate density (Spatially Explicit Capture-Recapture, SECR). We collected pellet groups …

W256 Composting Small Ruminants In Tennessee, Ricky Skillington

Animals/Livestock

Version 3.0

Regional Differences In Adipose Tissue Of The Sinclair Minipig, Suzanne Lauren Booker

Masters Theses

Adipose tissue is an endocrine organ, and its homeostatic mechanisms in normal weight, overweight and obese subjects must be elucidated. We sought to determine the basal adipose tissue biology of visceral (VIF) and subcutaneous (SQF) fat depots in 8 month old Sinclair minipigs, an animal that has been shown to be physiologically similar to humans.

Metabolic analysis showed a decrease in LDL, white blood cells (WBC), and lymphocyte percentages as the minipigs aged from 6 to 8 months (p <0.0001 and = 0.0046 and 0.0165 respectively). There were no significant changes in triglycerides, HDL, VLDL, and neutrophil percentages. There was a trend in insulin increase (P=0.0722).

Microarray analysis was performed to determine transcriptome differences between VIF and SQF. When VIF was compared to SQF, expression of a …

Spatial And Temporal Relationships Between Deer Harvest And Deer-Vehicle Collisions At Oak Ridge Reservation, Tennessee, Amanda Marie Pierce

Masters Theses

The Department of Energy Oak Ridge Reservation (ORR) and the nearby adjoining City of Oak Ridge, Tennessee had experienced a rise in deer-vehicle collisions (DVCs) to the point where safety for employees and residents became a concern. I investigated the effect of hunting, land cover, road mileage, season, lunar phase, sex, and change in traffic patterns that coincide with work shifts on DVCs from 1975 - 2008. The study area was divided into grids of 1.5 km² each for administration and data recording by managing agencies. Statistical analyses were performed on the ORR (121 grids) and GIS analyses were performed …

An Evaluation Of Quality Deer Management Programs In Tennessee, Christopher E. Shaw

Masters Theses

Several properties within Tennessee were managed under a quality deer management (QDM) philosophy from 1998 to 2006. Harvest characteristics of three private properties and three Wildlife Management Areas (WMAs) were compared to determine effects on buck harvest rates and the age structure and sex ratio of the harvest. Average annual buck harvest decreased at most areas following antler restrictions and ranged from 55 to 68% of pre-QDM levels because younger bucks were protected. Although the raw numbers showed an increase in older-aged bucks harvested following antler restrictions, when calculated on a per hunter or per permit issued basis, statistical increases …

An Experimental Release Of Elk Into Great Smoky Mountains National Park, Jennifer Lynn Murrow

Doctoral Dissertations

I conducted 6 years of field work to evaluate the habitat use and population dynamics of an experimental release of elk (Cervus elaphus) into Great Smoky Mountains National Park (Park). Elk exhibited relatively small home ranges (female: 10.4 km² and males: 22.4 km²) and movement distances decreased over time. I calculated survival rates (x = 0.73–0.93) and litter production rates (x = 0.73) for the population. To assess the potential for a long-term elk population, I incorporated those vital rates into the population modeling software Riskman and tested its sensitivity to any given …

Inventory And Analysis Of Plankton In Green River Within Mammoth Cave National Park, Justin H. Laughlin

Masters Theses

National Park Service biological staff are charged with preserving and protecting all creatures within a park’s boundaries. It is only a matter of time before exotic zebra mussels (Dreissena polymorpha) invade all eastern waterways including Green River within Mammoth Cave National Park, Kentucky. The National Park Service, in cooperation with University of Tennessee, initiated this inventory of plankton within Mammoth Cave National Park to establish baseline data prior to zebra mussel invasion. During this two year study (2000-2002), 180 plankton samples were collected at three sampling sites which encompassed all major riverine habitat types within the three flow zones (free-flow, …