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Fisheries Research Report No. 197 - Coastal And Marine Resource Condition Monitoring - Scoping Project, Brett A. Human, Justin I. Mcdonald

Fisheries research reports

Final NRM Report – Project 073007

Part 1– Knowledge review and gap analysis: Resource condition monitoring in the Pilbara and Kimberley regions of Western Australia

This report details extensive literature searches for both research and monitoring programs that have been undertaken in the marine and coastal environments of the Pilbara and Kimberley region of northern Western Australia. The Pilbara and Kimberley region is remote and difficult to access, resulting in very little monitoring effort of the marine and coastal environments in that region.

We currently know very little of the condition of marine and coastal natural resources in the Pilbara …

Investigating Saxitoxin Resistance In Softshell Clams (Mya Arenaria): Patterns Of Inheritance And Improvements On Methodology For Tracking And Identification, Scott A. Hamilton

Electronic Theses and Dissertations

Attempts to characterize and study the population dynamics of the softshell clam Mya arenaria in relation to a mutation which confers resistance to paralytic shellfish toxins are complicated by a lack of non-lethal genotyping techniques, reliable tagging methods and an understanding of the inheritance patterns of the marker. Presented here, is a straightforward and non-lethal technique for clam genotyping, a new method for the long term tagging of clams, and the offspring genotype frequencies from a number of pair matings between clams of known genotype. Hemolymph extracted from M. arenaria was used directly in a polymerase chain reaction (PCR) to …

Salinity And Stratification In The Gulf Of Maine: 2001-2008, Heather E. Deese-Riordan

Electronic Theses and Dissertations

The salinity and vertical density structure (stratification) of the Gulf of Maine strongly influence the physical and biological character of the region including: circulation and transport, vertical mixing, and primary productivity. Variability in salinity and stratification also provides insights into the character and timing of the oceanic waters entering the region, a key to predicting regional climate change. This thesis addresses outstanding questions related to variability in salinity and the relative role of salinity and temperature in creating stratification. Hourly observations from Ocean Observing System buoys throughout the Gulf provide the primary data source for this investigation. Analysis of estimated …

Summer/Fall 2009, Nsu Oceanographic Center

Currents

No abstract provided.

Changing Fecundity And Reproductive Output In Females Of A Chesapeake Bay Population Of Blue Crab, Callinectes Sapidus, Shannon L. Wells

OES Theses and Dissertations

The Chesapeake Bay blue crab, Callinectes sapidus, is an important species both ecologically and as a fishery. Fishery dependent and independent data indicate that the population declined abruptly in the mid-1990s and has remained low. Previous work prior to the decline in population abundance found a significant relationship between size and fecundity for mature females. As with many heavily fished populations, this population has experienced a reduction in the size at maturity. The objectives of this study were to reassess the fecundity of the population, determine the lipid and protein energy allocated for reproduction, and to examine the concentrations …

Spring 2009, Nsu Oceanographic Center

Currents

No abstract provided.

A Bio-Optical Model For Syringodium Filiforme Canopies, Margaret A. Stoughton

OES Theses and Dissertations

Seagrasses are significant ecological and biogeochemical agents in shallow water ecosystems throughout the world. In many regions, seagrass meadows occupy a sufficient fraction of the coastal zone, and generate optical signatures that can be observed from space. Bio-optical models of light absorption and scattering by submerged plant canopies for certain species such as Thalassia testudinum and Zostera marina have successfully modeled the plane irradiance distribution and photosynthesis within the submerged canopies. Syringodium filiforme differs &om T. testudinttm and Z marina, in leaf morphology and canopy architecture. The objective of this study was to develop a radiative transfer model that …

Factors Affecting Adult Foraging And Chick Growth Of Adélie Penguins (Pygoscelis Adeliae) Off The Western Antarctic Peninsula: A Modeling Study, Erik W. Chapman

OES Theses and Dissertations

Coupled individual-based models that simulate Adélie penguin ( Pygoscelis adeliae) chick growth and adult foraging behavior during the chick growth period were developed and used to explore factors that influence chick fledging mass and adult foraging energetics off the western Antarctic Peninsula (WAP). Adélie penguin colonies in this region are undergoing changes in population size that have been correlated with climate-driven alteration of the marine and terrestrial environment. Simulations were selected to highlight possible mechanistic linkages between climate variability and chick growth and adult foraging processes. These processes were the focus of this study because chicks that fledge at …

Characterization Of Antibiotic Resistance In Vibrio Cholerae Isolated From Ships' Ballast And Other Environmental Sources, Frank Kornegay Thomson Iii

OES Theses and Dissertations

Ships' ballast has been implicated as a vector in the dispersal of invasive species into new environments. Numerous cases have been documented for the introduction of macroorganisms via ballasting operations, but what is less known is the role of ballast water and residuals in the spread of potentially harmful microbes, especially with reference to novel genotypes. Of particular interest are the genes encoding for various forms of antibiotic resistance, many of which are carried on mobile genetic elements. A four-year sampling effort has yielded over 300 putative isolates of Vibrio cholerae from ships' ballast tanks and various environmental sources, of …

Identifying The Source Of Unknown Microcystin Genes And Predicting Microcystin Variants By Comparing Genes Within Uncultured Cyanobacterial Cells, C.J. Allender, G.R. Lecleir, J.M. Rinta-Kanto, R.L. Small, M.F. Satchwell, G.L. Boyer, Steven Wilhelm

Microbiology Publications and Other Works

While multiple phylogenetic markers have been used in the culture independent study of microcystin producing cyanobacteria, in only a few instances have multiple markers been studied within individual cells, and in all cases these studies have been conducted with cultured isolates. Here, we isolate and evaluate large DNA fragments (> 6 kb) encompassing two genes involved in microcystin biosynthesis (mcyA2 and mcyB1) and use them to identify the source of gene fragments found in water samples. Further investigation of these gene loci from individual cyanobacterial cells allowed for improved analysis of the genetic diversity within microcystin producers as …

Actinorhodopsin Genes Discovered Diverse In Freshwater Habitats And Among Cultivated Actinobacteria, A.K. Sharma, K. Sommerfeld, G.S. Bullerjahn, A.R. Matteson, Steven Wilhelm, J. Jezbera, U. Brandt, W.F. Doolittle, M.W. Hahn

Microbiology Publications and Other Works

Microbial rhodopsins are membrane proteins that utilize a retinal chromophore to harvest sunlight for energetic and photosensory functions. Recently, a group of novel rhodopsin sequences named ‘actinorhodopsins’ (ActRs) was hypothesized to exist among uncultured planktonic Actinobacteria. ActRs were discovered by mining metagenomic data obtained during the Venter Institute’s Global Ocean Sampling expedition, from a hypersaline lagoon, two estuaries and a freshwater lake. On the basis of these findings, and many studies that show Actinobacteria are common inhabitants of lakes, we predicted that ActR genes would likely be present in other freshwater habitats and among the genomes of cultivated Actinobacteria …

The Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: I. The Phytoplankton Community And Biogeochemical Response, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Y Zhang, G.R. Ditullio, P. Lee, Steven Wilhelm, J.M. Rowe, J. Sun, N. Nemcek, C. Gueguen, U. Passow, I. Benner, C. Brown, D.A. Hutchins

Microbiology Publications and Other Works

ABSTRACT: The North Atlantic spring bloom is one of the largest annual biological events in the ocean, and is characterized by dominance transitions from siliceous (diatoms) to calcareous (coccolithophores) algal groups. To study the effects of future global change on these phytoplankton and the biogeochemical cycles they mediate, a shipboard continuous culture experiment (Ecostat) was conducted in June 2005 during this transition period. Four treatments were examined: (1) 12°C and 390 ppm CO₂ (ambient control), (2) 12°C and 690 ppm CO₂ (high pCO₂), (3) 16°C and 390 ppm CO₂ (high temperature), and (4) 16°C and …

Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: Iii. Dimethylsulfoniopropionate, P.A. Lee, J.R. Rudisill, A.R. Neeley, D.A. Hutchins, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Steven Wilhelm, J.M. Rowe, G.R. Ditullio

Microbiology Publications and Other Works

The CLAW hypothesis argues that a negative feedback mechanism involving phytoplankton-derived dimethylsulfoniopropionate (DMSP) could mitigate increasing sea surface temperatures that result from global warming. DMSP is converted to the climatically active dimethylsulfide (DMS), which is transferred to the atmosphere and photochemically oxidized to sulfate aerosols, leading to increases in planetary albedo and cooling of the Earth’s atmosphere. A shipboard incubation experiment was conducted to investigate the effects of increased temperature and pCO2 on the algal community structure of the North Atlantic spring bloom and their subsequent impact on particulate and dissolved DMSP concentrations (DMSP_p and DMSP_d). Under …

Andrews Hall And Seawater Research Laboratory Dedication, Virginia Institute Of Marine Science; College Of William And Mary

Miscellaneous

Dedication: April 16, 2009. Program of events.

Winter 2009, Nsu Oceanographic Center

Currents

No abstract provided.

Actinorhodopsin Genes Discovered Diverse In Freshwater Habitats And Among Cultivated Actinobacteria, A.K. Sharma, K. Sommerfeld, G.S. Bullerjahn, A.R. Matteson, Steven Wilhelm, J. Jezbera, U. Brandt, W.F. Doolittle, M.W. Hahn

Steven Wilhelm

Microbial rhodopsins are membrane proteins that utilize a retinal chromophore to harvest sunlight for energetic and photosensory functions. Recently, a group of novel rhodopsin sequences named ‘actinorhodopsins’ (ActRs) was hypothesized to exist among uncultured planktonic Actinobacteria. ActRs were discovered by mining metagenomic data obtained during the Venter Institute’s Global Ocean Sampling expedition, from a hypersaline lagoon, two estuaries and a freshwater lake. On the basis of these findings, and many studies that show Actinobacteria are common inhabitants of lakes, we predicted that ActR genes would likely be present in other freshwater habitats and among the genomes of cultivated Actinobacteria …

Identifying The Source Of Unknown Microcystin Genes And Predicting Microcystin Variants By Comparing Genes Within Uncultured Cyanobacterial Cells, C.J. Allender, G.R. Lecleir, J.M. Rinta-Kanto, R.L. Small, M.F. Satchwell, G.L. Boyer, Steven Wilhelm

Steven Wilhelm

While multiple phylogenetic markers have been used in the culture independent study of microcystin producing cyanobacteria, in only a few instances have multiple markers been studied within individual cells, and in all cases these studies have been conducted with cultured isolates. Here, we isolate and evaluate large DNA fragments (> 6 kb) encompassing two genes involved in microcystin biosynthesis (mcyA2 and mcyB1) and use them to identify the source of gene fragments found in water samples. Further investigation of these gene loci from individual cyanobacterial cells allowed for improved analysis of the genetic diversity within microcystin producers as …

Distribution Of Calcifying And Silicifying Phytoplankton In Relation To Environmental And Biogeochemical Parameters During The Late Stages Of The 2005 North East Atlantic Spring Bloom, K. Leblanc, C.E. Hare, Y. Feng, G.M. Berg, G.R. Ditullio, A. Neeley, I. Benner, C. Sprengel, A. Beck, S.A. Sanudo-Wilhemy, U. Passow, K. Klinck, J.M. Rowe, Steven Wilhelm, C.W. Brown, D.A. Hutchins

Steven Wilhelm

The late stage of the North East Atlantic (NEA) spring bloom was investigated during June 2005 along a transect section from 45 to 66° N between 15 and 20° W in order to characterize the contribution of siliceous and calcareous phytoplankton groups and describe their distribution in relation to environmental factors. We measured several biogeochemical parameters such as nutrients, surface trace metals, algal pigments, biogenic silica (BSi), particulate inorganic carbon (PIC) or calcium carbonate, particulate organic carbon, nitrogen and phosphorus (POC, PON and POP, respectively), as well as transparent exopolymer particles (TEP). Results were compared with other studies undertaken in …

The Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: I. The Phytoplankton Community And Biogeochemical Response, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Y Zhang, G.R. Ditullio, P. Lee, Steven Wilhelm, J.M. Rowe, J. Sun, N. Nemcek, C. Gueguen, U. Passow, I. Benner, C. Brown, D.A. Hutchins

Steven Wilhelm

ABSTRACT: The North Atlantic spring bloom is one of the largest annual biological events in the ocean, and is characterized by dominance transitions from siliceous (diatoms) to calcareous (coccolithophores) algal groups. To study the effects of future global change on these phytoplankton and the biogeochemical cycles they mediate, a shipboard continuous culture experiment (Ecostat) was conducted in June 2005 during this transition period. Four treatments were examined: (1) 12°C and 390 ppm CO₂ (ambient control), (2) 12°C and 690 ppm CO₂ (high pCO₂), (3) 16°C and 390 ppm CO₂ (high temperature), and (4) 16°C and …

Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: Iii. Dimethylsulfoniopropionate, P.A. Lee, J.R. Rudisill, A.R. Neeley, D.A. Hutchins, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Steven Wilhelm, J.M. Rowe, G.R. Ditullio

Steven Wilhelm

The CLAW hypothesis argues that a negative feedback mechanism involving phytoplankton-derived dimethylsulfoniopropionate (DMSP) could mitigate increasing sea surface temperatures that result from global warming. DMSP is converted to the climatically active dimethylsulfide (DMS), which is transferred to the atmosphere and photochemically oxidized to sulfate aerosols, leading to increases in planetary albedo and cooling of the Earth’s atmosphere. A shipboard incubation experiment was conducted to investigate the effects of increased temperature and pCO2 on the algal community structure of the North Atlantic spring bloom and their subsequent impact on particulate and dissolved DMSP concentrations (DMSP_p and DMSP_d). Under …

Distribution Of Calcifying And Silicifying Phytoplankton In Relation To Environmental And Biogeochemical Parameters During The Late Stages Of The 2005 North East Atlantic Spring Bloom, K. Leblanc, C.E. Hare, Y. Feng, G.M. Berg, G.R. Ditullio, A. Neeley, I. Benner, C. Sprengel, A. Beck, S.A. Sanudo-Wilhemy, U. Passow, K. Klinck, J.M. Rowe, Steven Wilhelm, C.W. Brown, D.A. Hutchins

Microbiology Publications and Other Works

The late stage of the North East Atlantic (NEA) spring bloom was investigated during June 2005 along a transect section from 45 to 66° N between 15 and 20° W in order to characterize the contribution of siliceous and calcareous phytoplankton groups and describe their distribution in relation to environmental factors. We measured several biogeochemical parameters such as nutrients, surface trace metals, algal pigments, biogenic silica (BSi), particulate inorganic carbon (PIC) or calcium carbonate, particulate organic carbon, nitrogen and phosphorus (POC, PON and POP, respectively), as well as transparent exopolymer particles (TEP). Results were compared with other studies undertaken in …

Synergistic Effects Of Iron And Temperature On Antarctic Phytoplankton And Microzooplankton Assemblages, J. M. Rose, Y. Feng, Et Al, Walker O. Smith Jr., B. Sigist, S. Tozzi, Et Al

VIMS Articles

Iron availability and temperature are important limiting factors for the biota in many areas of the world ocean, and both have been predicted to change in future climate scenarios. However, the impacts of combined changes in these two key factors on microbial trophic dynamics and nutrient cycling are unknown. We examined the relative effects of iron addition (+1 nM) and increased temperature (+4 degrees C) on plankton assemblages of the Ross Sea, Antarctica, a region characterized by annual algal blooms and an active microbial community. Increased iron and temperature individually had consistently significant but relatively minor positive effects on total …

Anthropogenic Causes Of Copepod Mortality And Bacterial Decomposition Of Copepod Carcasses, Samantha L. Bickel

Dissertations, Theses, and Masters Projects

Although zooplankton carcasses can be quite prevalent within aquatic systems, they have largely been overlooked in most zooplankton population studies. Anthropogenic stressors can potentially increase the overall abundance of carcasses on a local scale. Once a carcass is present within a system, the fate of its biomass is of considerable interest as it may be remineralized within the water column or transported to depth. Through the collection of field samples I assessed the possibility of an anthropogenic stressor (boat-generated turbulence) as a potential source of nonconsumptive mortality. I also conducted a series of laboratory experiments to monitor the decomposition of …

Vims Response To Climate Change 2009, United Nations Environmental Programme : Responding To Climate Change In The Coastal Zone, Virginia Institute Of Marine Science. Initiative For Coastal Climate Change Research

Reports

VIMS Response to Climate Change 2009 United Nations Environmental Program Responding to climate change in the coastal zone

Climate Change And Submerged Aquatic Vegetation In Virginia, Kenneth A. Moore, Robert J. Orth, Virginia Institute Of Marine Science. Initiative For Coastal Climate Change Research

Reports

VIMS climate change white paper: Climate change and submerged aquatic vegetation in Virginia

Planning For Sea Level Rise And Coastal Flooding, John D. Boon, Harry V. Wang, Jian Shen, Virginia Institute Of Marine Science. Initiative For Coastal Climate Change Research

Reports

VIMS climate change white papers: Planning for sea level rise and coastal flooding

Observing And Modeling Global Warming Impacts In Virginia, Mark R. Patterson, Mark Brush, Marjorie A.M. Friedrichs, Virginia Institute Of Marine Science. Initiative For Coastal Climate Change Research

Reports

VIMS climate change white paper: Observing and modeling global warming impacts in Virginia

Marine Plankton Food Webs And Climate Change, Walker O. Smith Jr., Deborah K. Steinberg, Deborah A. Bronk, Kam W. Tang, Virginia Institute Of Marine Science. Initiative For Coastal Climate Change Research

Reports

VIMS climate change white papers: Marine plankton food webs and climate change

Land-Coast Connections And Climate Change : Carbon Cycling In Chesapeake Bay And Its Watershed, Elizabeth A. Canuel, James E. Bauer, Virginia Institute Of Marine Science, Initiative For Coastal Climate Change Research

Reports

VIMS climate change white paper: Land-coast connections and climate change : carbon cycling in Chesapeake Bay and its watershed

Vanishing And Emerging Ecosystems Of Coastal Virginia : Climate Change Impacts And Adaptation, Emmett J. Duffy, Virginia Institute Of Marine Science. Initiative For Coastal Climate Change Research

Reports

VIMS climate change white papers: Vanishing and emerging ecosystems of coastal Virginia : climate change impacts and adaptation