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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler
IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt
Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …
A Comparison Of Adenosine Triphosphate With Other Metrics Of Microbial Biomass In A Gradient From The North Atlantic To The Chesapeake Bay, Alexander B. Bochdansky, Amber A. Beecher, Joshua R. Calderon, Alison N. Stouffer, Nyjaee N. Washington
A Comparison Of Adenosine Triphosphate With Other Metrics Of Microbial Biomass In A Gradient From The North Atlantic To The Chesapeake Bay, Alexander B. Bochdansky, Amber A. Beecher, Joshua R. Calderon, Alison N. Stouffer, Nyjaee N. Washington
OES Faculty Publications
A new, simplified protocol for determining particulate adenosine triphosphate (ATP) levels allows for the assessment of microbial biomass distribution in aquatic systems at a high temporal and spatial resolution. A comparison of ATP data with related variables, such as particulate carbon, nitrogen, chlorophyll, and turbidity in pelagic samples, yielded significant and strong correlations in a gradient from the tributaries of the Chesapeake Bay (sigma-t = 8) to the open North Atlantic (sigma-t = 29). Correlations varied between ATP and biomass depending on the microscopic method employed. Despite the much greater effort involved, biomass determined by microscopy correlated poorly with other …
Molecular Detection Of Pathogenic Leptospira And Microbial Source Tracking Of Fecal Pollution In San Juan, Puerto Rico, Taylor I. Chapman
Molecular Detection Of Pathogenic Leptospira And Microbial Source Tracking Of Fecal Pollution In San Juan, Puerto Rico, Taylor I. Chapman
Biology Theses
Leptospirosis, caused by pathogenic Leptospira, is endemic to tropical regions. Leptospira is released into the environment through the secretion of urine from animals, making it easily transmissible through water sources. The estuarian environment surrounding the area of San Juan, Puerto Rico and its high density of urban development creates ideal conditions for transmission of Leptospirosis. The goal of this study was to determine the presence of Leptospira in these surface waters and use Microbial Source Tracking (MST) to identify the possible source of pathogenic Leptospira. Eighty-seven water samples were collected during the dry (44) and wet (43) seasons. …
Distinct Distribution Of Archaea From Soil To Freshwater To Estuary: Implications Of Archaeal Composition And Function In Different Environments, Hualong Wang, Raven Bier, Laura Zgleszewski, Marc Peipoch, Emmanuel Omondi, Atanu Mukherjee, Feng Chen, Chuanlun Zhang, Jinjun Kan
Distinct Distribution Of Archaea From Soil To Freshwater To Estuary: Implications Of Archaeal Composition And Function In Different Environments, Hualong Wang, Raven Bier, Laura Zgleszewski, Marc Peipoch, Emmanuel Omondi, Atanu Mukherjee, Feng Chen, Chuanlun Zhang, Jinjun Kan
Agricultural and Environmental Sciences Faculty Research
In addition to inhabiting extreme territories, Archaea are widely distributed in common environments spanning from terrestrial to aquatic environments. This study investigated and compared archaeal community structures from three different habitats (representing distinct environments): agriculture soils (from farming system trials FST, PA, United States), freshwater biofilms (from White Clay Creek, PA, United States), and estuary water (Chesapeake Bay, United States). High-throughput sequencing of 16S rRNA genes indicated that Thaumarchaeota, Euryarchaeota, Nanoarchaeota, Crenarchaeota, and Diapherotrites were the commonly found dominant phyla across these three environments. Similar to Bacteria, distinct community structure and distribution patterns for Archaea were observed in soils vs. …
Evidence For The Priming Effect In A Planktonic Estuarine Microbial Community, Andrew D. Steen, Lauren N.M. Quigley, Alison Buchan
Evidence For The Priming Effect In A Planktonic Estuarine Microbial Community, Andrew D. Steen, Lauren N.M. Quigley, Alison Buchan
Microbiology Publications and Other Works
The “priming effect,” in which addition of labile substances changes the remineralization rate of recalcitrant organic matter, has been intensively studied in soils, but is less well-documented in aquatic systems. We investigated the extent to which additions of nutrients or labile organic carbon could influence remineralization rates of 14C-labeled, microbially-degraded, phytoplankton-derived organic matter (OM) in microcosms inoculated with microbial communities drawn from Grove Creek Estuary in coastal Georgia, USA. We found that amendment with labile protein plus phosphorus increased remineralization rates of degraded, phytoplankton-derived OM by up to 100%, whereas acetate slightly decreased remineralization rates relative to an unamended control. …
Estuarine Nitrifiers: New Players, Patterns And Processes, Anne E. Bernhard, Annette Bollmann
Estuarine Nitrifiers: New Players, Patterns And Processes, Anne E. Bernhard, Annette Bollmann
Biology Faculty Publications
Ever since the first descriptions of ammonia-oxidizing Bacteria by Winogradsky in the late 1800s, the metabolic capability of aerobic ammonia oxidation has been restricted to a phylogenetically narrow group of bacteria. However, the recent discovery of ammonia-oxidizing Archaea has forced microbiologists and ecologists to re-evaluate long-held paradigms and the role of niche partitioning between bacterial and archaeal ammonia oxidizers. Much of the current research has been conducted in open ocean or terrestrial systems, where community patterns of archaeal and bacterial ammonia oxidizers are highly congruent. Studies of archaeal and bacterial ammonia oxidizers in estuarine systems, however, present a very different …