Life Sciences Commons^™

Effects Of Trypanosoma Musculi Kendall, 1906 Immune Serum On The Reproduction Of Trichinella Spiralis (Owen, 1835) Railliet, 1896 In Mice, Samuel L. Chafin

Loma Linda University Electronic Theses, Dissertations & Projects

The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A …

Effect Of Time And Diet On The Immune Status Of Mice Challenged With Herpes-Transformed Cells, Daila S. Gridley

Loma Linda University Electronic Theses, Dissertations & Projects

Human Herpes simplex virus type 2 (HSV 2)-transformed Balb/c mouse cells (H238 cells) are malignant in Balb/c mice. The immune stratus of mice bearing H238 cell-induced tumors was studied and changes in immune response with time and with altered diet of the mice were measured.

In the experiments dealing with the status of immunity with time after tumor cell injection, mice were fed Purina Laboratory Chow (P.L.C.), injected with H238 cells, and sacrificed three to eight weeks post-injection. In the diet experiments, groups of mice were fed either P.L.C. or one of six equicaloric diets, consisting of low or high …

Non-Parental-Type Recombinants In Crosses Between Different Restriction-Modification Systems In Salmonella, Mary Ball

Loma Linda University Electronic Theses, Dissertations & Projects

Many new restriction-modification (R-M) system which are closely linked to serB and allelic to the hsd_SB system of S. typimurium occur in different Salmonella serotypes. In PI transduction studies with these systems, recombinants were isolated which fell into one of three classes with reference to their specific restriction phenotype. Recombinants that restricted like the donor, recombinants that restricted like the recipient and recombinants that had lost the ability to restrict. This latter group having lost the recipient SB restriction without gaining the specific donor restriction and here called "zero recombinants,” were of special interest and were investigated.

Zero recombinants …

Reactivity Of Antibodies Versus Streptococcal Carbohydrate And Protein Antigens And Human Glomerular Basement Membrane Carbohydrate And Protein Antigens In Mammalian Tissues, Kathleen A. Chase

Master's Theses

No abstract provided.

Characterization Of The Nonfermentative Gram Negative Rods Of Group Iik Utilizing Biochemical, Serological, And Antibiotic Susceptibility Tests, Donna S. Granstrom

Master's Theses

No abstract provided.

Characterization Of Pseudomonas Cepacia Using Biochemical, Antibiotic Susceptibility And Serological Tests, Beatrice Grazina Miceika

Master's Theses

No abstract provided.

Cytology And Physiology Of Arthrospore Formation In The Dermatophyte Trichophyton Mentagrophytes, Ruth Gail Emyanitoff

Dissertations

No abstract provided.

In Vivo And In Vitro Interactions Between Bacteroides Fragilis And Escherichia Coli: Their Role In Intra-Abdominal Abscess Formation, James Carl Hagen

Dissertations

No abstract provided.

Listeria Monocytogenes In The Environment Of Livestock In South Dakota, James W. Neiles

Electronic Theses and Dissertations

The purpose of this investigation is to study the incidence of Listeria monocytogenes in the environment of livestock in South Dakota. The data accumulated from these studies will provide information as to its natural habitat, mode of transmission and factors responsible for its incidence.

Methodology Development For The Study Of The Anaerobic Bacterial Flora Of Bovine Feces, Donald G. Kirsch

Electronic Theses and Dissertations

The intestinal bacterial flora of an animal has a considerable influence on the physiology of that animal. Some bacteria of the intestine are pathogenic and can initiate infections if they gain access to a susceptible individual. Some of the intestinal bacteria can produce potent carcinogens in vitro and can presumably do this also in vivo. Food passing through the alimentary tract of an animal is also chemically modified through the enzymatic action of the bacterial flora. The animal is also protected from some enteric infections through the antagonistic and competitive actions of the established organisms of the normal bacterial flora. …

Rescue Of Infectious Bk Virus From A T-Antigen Negative Bkv Transformed Bhk-21 Cell Line, David Michael Olive

Dissertations

No abstract provided.

An Electron Microscopic Investigation Of Bovine Abortion, Hans A. Draayer

Electronic Theses and Dissertations

Bovine abortion continues to cause economic loss to farmers and ranchers. In South Dakota, monetary loss from bovine abortion has been estimated at 4 to 4.8 million dollars annually. (31) Brucellosis was once considered the major cause of bovine abortion. Since the prevalence of brucellosis has been reduced to less than 1% of the total United States cattle population, there has been an increase in demand for laboratory assistance in the diagnosis of the causes of bovine abortion. With the present day technology in microbiology, histology, and serology, a confirmed identification of the causative agent of bovine abortion can be …

Interactions In Lambdoid Bacteriophage Populations, Bruce Edward Mitchell

Masters Theses

The lambdoid bacteriophages are ideal subjects for the study of populational interactions at the molecular level because they do not mutually exclude one another in mixed infection of the same host cell. Previous work done in this laboratory using the lambdoid phage pairs λ-434 hy mi and λ-∅80 have demonstrated a density-dependent reduction in progeny yield per infected cell (burst size) as a function of increasing multiplicity of infection (m.o.i.). The λ cI gene which codes for the repress or molecule has been demonstrated to be the cause of the interaction in the λ-434 hy mi phage pair. …

The Effects Of Residual Chlorine On Selected Microflora Of Kickapoo And Cassell Creek, James A. Mcgaughey

Masters Theses

The survival of fecal coliforms, fecal streptococci, and heterotrophs after exposure to chlorine were compared in receiving waters of the Mattoon and Charleston, Illinois waste treatment facilities employing membrane filter techniques and Standard Plate Counts. Water samples were taken from receiving waters upstream from the treatment plant, from secondary effluent and chlorinated secondary effluent within the plant, and from downstream receiving waters. Stream flow contribution and plant effluent contribution required the calculation of dilution factors to determine expected numbers of the various microflora found downstream assuming no residual chlorine effect. Total chlorine concentration was measured by amperometric titration at the …

Rotaviral Enteritis In Gnotobiotic Pigs: A Scanning Electron Microscopic Study, Marcus W. Johnshoy

Electronic Theses and Dissertations

The objectives of this study were: 1. To experimentally reproduce rotaviral diarrhea in gnotobiotic pigs. 2. To determine the appearance of the normal intestine by scanning electron and light microscopy. 3. To compare by scanning electron and light microscopy the appearance of segments from different areas of intestine and to determine the development, location, and severity of rotaviral lesions in gnotobiotic pigs.

Nonsporing Anaerobic Bacteria In Foods, Sylvia M. Huppler

Electronic Theses and Dissertations

Anaerobic non spore forming bacteria are not detected in routine food examinations, and their occurrences in foods has seldom been reported in the literature. The purpose of this study is to determine the occurrence of non-spore forming anaerobes, the identification of the isolates found, and the possible growth and multiplication in foods which might contribute to spoilage or food illness.

Partial Characterization And Comparison Of Rhizobia From Astragalus Flexuosus Glycyrrhiza Lepidota, And Trifolium Spp., Elmer L. Eide

Electronic Theses and Dissertations

Biological nitrogen fixation, a means of alleviating our increasing dependence on synthesized nitrogen fertilizers, is being studied with renewed interest. Production of nitrogen fertilizer requires large amounts of natural gas and other non-renewable energy sources, while the energy for biological nitrogen fixation is derived either directly or indirectly from photosynthesis. With only limited supplies of natural gas and an increasing population, and thus increasing demand for food, the advantages and even necessity of understanding and enhancing biological nitrogen is obvious. The nitrogenase enzyme complex which catalyzes the reduction of dinitrogen to ammonia is possessed by a limited number of species …

Partial Purification Of Heat Stable Enterotoxin Produced By Escherichia Coli Isolated From A Case Of Porcine Colibacillosis, Lee Ann Zochert Swanson

Electronic Theses and Dissertations

Colibacillosis (CB) in livestock is an economically important disease caused by the bacterium Escherichia coli. It is estimated that nearly one third of all baby pigs born in South Dakota die prior to weaning, and one fourth of these deaths is believed to be caused by CB (62). Similarly, it has been stated that 6 to 20% of calves born alive die from this disease (3). The term colibacillosis encompasses a group of diseases caused by E. coli in neonatal and weaned animals and humans (3). The several different types of CB are due to the differences in tissue localization …

Bacterial Response To Crude Oil Spillage In A Salt Marsh, Russell Paul Herwig

Dissertations, Theses, and Masters Projects

No abstract provided.