Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
A Cell Cycle-Regulated Adenine Dna Methyltransferase From Caulobacter Crescentus Processively Methylates Gantc Sites On Hemimethylated Dna, Craig Stephens, Anthony J. Berdis, Irene Lee, James K. Coward, Rachel Wright, Lucy Shapiro, Stephen J. Benkovic
A Cell Cycle-Regulated Adenine Dna Methyltransferase From Caulobacter Crescentus Processively Methylates Gantc Sites On Hemimethylated Dna, Craig Stephens, Anthony J. Berdis, Irene Lee, James K. Coward, Rachel Wright, Lucy Shapiro, Stephen J. Benkovic
Biology
The kinetic properties of an adenine DNA methyltransferase involved in cell cycle regulation of Caulobacter crescentus have been elucidated by using defined unmethylated or hemimethylated DNA (DNAHM) substrates. Catalytic efficiency is significantly enhanced with a DNAHM substrate. Biphasic kinetic behavior during methyl incorporation is observed when unmethylated or DNAHM substrates are used, indicating that a step after chemistry limits enzyme turnover and is most likely the release of enzyme from methylated DNA product. The enzyme is thermally inactivated at 30 degrees C within 20 min; this process is substantially decreased in the presence of saturating concentrations of DNAHM, suggesting that …