Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- 3t3 cells (1)
- Actins (1)
- Animals (1)
- Bacteriology (1)
- Bacterioplankton (1)
-
- Blue-green bacteria (1)
- Caulobacter crescentus (1)
- Cbfb protein (1)
- Cbfbeta-myh11 fusion protein (1)
- Cell nucleus (1)
- Chondrocrania; anatomy; larvae; Leptodactylus; variation; phylogeny (1)
- Chromosomes (1)
- Core binding fac (1)
- Core binding factor alpha 1 subunit (1)
- Core binding factor alpha 2 subunit (1)
- Core binding factor beta subunit (1)
- Core binding factors (1)
- Crickets (1)
- Cyanobacteria (1)
- Cytoskeleton (1)
- DNA relication (1)
- DNA sequence (1)
- Developmental biology (1)
- Dictyostelium discoideum (1)
- Dna-binding proteins (1)
- Esterase (1)
- Fecal Bacteria (1)
- Fecal Coliform (1)
- Fluorescent antibody technique (1)
- Fusion (1)
Articles 1 - 29 of 29
Full-Text Articles in Life Sciences
The Leukemic Protein Core Binding Factor Beta (Cbfbeta)-Smooth-Muscle Myosin Heavy Chain Sequesters Cbfalpha2 Into Cytoskeletal Filaments And Aggregates, Neeraj Adya, Terryl Stacy, Nancy A. Speck, Pu Paul Liu
The Leukemic Protein Core Binding Factor Beta (Cbfbeta)-Smooth-Muscle Myosin Heavy Chain Sequesters Cbfalpha2 Into Cytoskeletal Filaments And Aggregates, Neeraj Adya, Terryl Stacy, Nancy A. Speck, Pu Paul Liu
Dartmouth Scholarship
The fusion gene CBFB-MYH11 is generated by the chromosome 16 inversion associated with acute myeloid leukemias. This gene encodes a chimeric protein involving the core binding factor β (CBFβ) and the smooth-muscle myosin heavy chain (SMMHC). Mouse model studies suggest that this chimeric protein CBFβ-SMMHC dominantly suppresses the function of CBF, a heterodimeric transcription factor composed of DNA binding subunits (CBFα1 to 3) and a non-DNA binding subunit (CBFβ). This dominant suppression results in the blockage of hematopoiesis in mice and presumably contributes to leukemogenesis. We used transient-transfection assays, in combination with immunofluorescence and green fluorescent protein-tagged proteins, to monitor …
Identification And Characterization Of Spcu, A Chaperone Required For Efficient Secretion Of The Exou Cytotoxin, Viviane Finck-Barbançon, Timothy L. Yahr, Dara W. Frank
Identification And Characterization Of Spcu, A Chaperone Required For Efficient Secretion Of The Exou Cytotoxin, Viviane Finck-Barbançon, Timothy L. Yahr, Dara W. Frank
Dartmouth Scholarship
In recent studies, we have shown that Pseudomonas aeruginosa strains that are acutely cytotoxic in vitro damage the lung epithelium in vivo. Genetic analysis indicated that the factor responsible for acute cytotoxicity was controlled by ExsA and therefore was part of the exoenzyme S regulon. The specific virulence determinant responsible for epithelial damage in vivo and cytotoxicity in vitro was subsequently mapped to the exoU locus. The present studies are focused on a genetic characterization of the exoU locus. Northern blot analyses and complementation experiments indicated that a region downstream of exoU was expressed and that the expression of this …
Fecal Bacteria Survival And Infiltration Through A Shallow Agricultural Soil: Timing And Tillage Effects, C. S. Stoddard, Mark S. Coyne, John H. Grove
Fecal Bacteria Survival And Infiltration Through A Shallow Agricultural Soil: Timing And Tillage Effects, C. S. Stoddard, Mark S. Coyne, John H. Grove
Plant and Soil Sciences Faculty Publications
Human and livestock exposure to fecal pathogens via contaminated surface or groundwater is an important water quality concern for soils receiving animal wastes. The effects of manure application timing (spring or fall application) and soil management (no-tillage or conservation tillage) on fecal bacteria infiltration through shallow karst soils in central Kentucky (the Bluegrass region) have not been evaluated. We performed a field experiment to measure fecal coliforms and fecal streptococci in leachate from dairy manure-amended no-tillage and conservation tillage soils. Manure significantly increased fecal bacteria in leachate compared with unmanured treatments. After manure application, the leachate that collected in zero-tension …
The Seca Subunit Of Escherichia Coli Preprotein Translocase Is Exposed To The Periplasm, Jerry Eichler, William Wickner
The Seca Subunit Of Escherichia Coli Preprotein Translocase Is Exposed To The Periplasm, Jerry Eichler, William Wickner
Dartmouth Scholarship
SecA undergoes conformational changes during translocation, inserting domains into and across the membrane or enhancing the protease resistance of these domains. We now show that some SecA bound at SecYEG is accessible from the periplasm to a membrane-impermeant probe in cells with a permeabilized outer membrane but an intact plasma membrane.
Vertical Profiles Of Virus-Like Particles And Bacteria In The Water Column And Sediments Of Chesapeake Bay, Usa, Lisa A. Drake, Keun-Hyung Choi, A. G. Edward Haskell, Fred C. Dobbs
Vertical Profiles Of Virus-Like Particles And Bacteria In The Water Column And Sediments Of Chesapeake Bay, Usa, Lisa A. Drake, Keun-Hyung Choi, A. G. Edward Haskell, Fred C. Dobbs
OES Faculty Publications
Vertical profiles of virus-like particles (VLPs) and bacteria were determined by near-synoptic sampling through the water column and 15 to 25 cm into the sediment at 5 stations across the mouth of Chesapeake Bay, USA. VLPs were about 10 times more abundant in the pore water (grand mean = 3.6 x 10(8) VLPs ml(-1)) than in the water column (grand mean = 3.8 x 10(7) VLPs ml(-1)). Similarly, bacteria counts were about 3 times higher in the pore water (grand mean = 6.5 x 10(6) bacteria ml(-1)) than in the water column (grand mean = 2.4 x 10(6) bacteria ml(-1)). …
Influence Of The Ccr2-V64i Polymorphism On Human Immunodeficiency Virus Type 1 Coreceptor Activity And On Chemokine Receptor Function Of Ccr2b, Ccr3, Ccr5, And Cxcr4, Benhur Lee, Benjamin J. Doranz, Shalini Rana, Yanji Yi, Mario Mellado, Jose M. R. Frade, Carlos Martinez-A., Stephen J. O'Brien, Michael Dean, Ronald G. Collman, Robert W. Doms
Influence Of The Ccr2-V64i Polymorphism On Human Immunodeficiency Virus Type 1 Coreceptor Activity And On Chemokine Receptor Function Of Ccr2b, Ccr3, Ccr5, And Cxcr4, Benhur Lee, Benjamin J. Doranz, Shalini Rana, Yanji Yi, Mario Mellado, Jose M. R. Frade, Carlos Martinez-A., Stephen J. O'Brien, Michael Dean, Ronald G. Collman, Robert W. Doms
Biology Faculty Articles
The chemokine receptors CCR5 and CXCR4 are used by human immunodeficiency virus type 1 (HIV-1) in conjunction with CD4 to infect cells. In addition, some virus strains can use alternative chemokine receptors, including CCR2b and CCR3, for infection. A polymorphism in CCR2 (CCR2-V64I) is associated with a 2- to 4-year delay in the progression to AIDS. To investigate the mechanism of this protective effect, we studied the expression of CCR2b and CCR2b-V64I, their chemokine and HIV-1 coreceptor activities, and their effects on the expression and receptor activities of the major HIV-1 coreceptors. CCR2b and CCR2b-V64I were expressed at …
Cytoplasmic Assembly And Accumulation Of Human Immunodeficiency Virus Types 1 And 2 In Recombinant Human Colony-Stimulating Factor-1-Treated Human Monocytes: An Ultrastructural Study, Jan Marc Orenstein, Monte S. Meltzer, Terri Phipps, Howard Gendelman
Cytoplasmic Assembly And Accumulation Of Human Immunodeficiency Virus Types 1 And 2 In Recombinant Human Colony-Stimulating Factor-1-Treated Human Monocytes: An Ultrastructural Study, Jan Marc Orenstein, Monte S. Meltzer, Terri Phipps, Howard Gendelman
Nebraska Center for Virology: Faculty Publications
Recombinant human colony-stimulating factor-1-treated human peripheral blood-derived monocytes-macrophages are efficient host cells for recovery of the human immunodeficiency virus (HIV) from blood leukocytes of patients with acquired immunodeficiency syndrome. These cells can be maintained as viable monolayers for intervals exceeding 3 months. Infection with HIV resulted in virus-induced cytopathic effects, accompanied by relatively high levels of released progeny virus, followed by a prolonged low-level release of virus from morphologically normal cells. In both acutely and chronically infected monocytes, viral particles were seen budding into and accumulating within cytoplasmic vacuoles. The number of intravacuolar virions far exceeded those associated with the …
The Physiology Of Life-History Trade-Offs: Experimental Analysis Of A Hormonally Induced Life-History Trade-Off In Gryllus Assimilis, Anthony J. Zera, Jason Potts, Kari Kobus
The Physiology Of Life-History Trade-Offs: Experimental Analysis Of A Hormonally Induced Life-History Trade-Off In Gryllus Assimilis, Anthony J. Zera, Jason Potts, Kari Kobus
Anthony Zera Publications
Adult Gryllus assimilis given an analog of juvenile hormone exhibited reduced flight muscles and enlarged ovaries similar to those found in naturally occurring flightless individuals of species that are polymorphic for dispersal capability. Control and hormone-treated (flightless) G. assimilis did not differ in the amount of food consumed or assimilated on any of three diets that differed in nutrient quantity. Thus, enhanced ovarian growth of flightless individuals resulted from increased allocation of internal nutrients to reproduction (i.e., a trade-off) rather than from increased acquisition of nutrients. Compared with flight-capable controls, flightless G. assimilis also had reduced whole-organism respiration, reduced respiration …
Ultraviolet Radiation Triggers The Ribotoxic Stress Response In Mammalian Cells, Mihail S. Iordanov, David Pribnow, Jennifer L. Magun, Thanh-Hoai Dinh, Jean A. Pearson, Bruce E. Magun
Ultraviolet Radiation Triggers The Ribotoxic Stress Response In Mammalian Cells, Mihail S. Iordanov, David Pribnow, Jennifer L. Magun, Thanh-Hoai Dinh, Jean A. Pearson, Bruce E. Magun
CUP Faculty Research
The ribotoxic stress response, which is conserved between prokaryotes and eukaryotes, is a cellular reaction to cytotoxic interference with the function of the 3′-end of the large (23 S/28 S) ribosomal RNA. The 3′-end of the large rRNA is directly involved in the three sequential steps of translational elongation: the aminoacyl-tRNA binding, the peptidyl transfer, and the ribosomal translocation. In mammalian cells, the ribotoxic stress response involves activation of the stress-activated protein kinase/c-Jun NH2-terminal kinase and the p38 mitogen-activated protein kinase and transcriptional induction of immediate early genes such as c-fos and c-jun. Active ribosomes …
Detection Of Mycobacterium Tuberculosis In Paraffin Embedded Intestinal Tissue Specimens By Polymerase Chain Reaction: Characterization Of Is6110 Element Negative Strains, Tariq Moatter, Shaper Mirza, Mohammad Shahid Siddiqui, Irshad Nabi Soomro
Detection Of Mycobacterium Tuberculosis In Paraffin Embedded Intestinal Tissue Specimens By Polymerase Chain Reaction: Characterization Of Is6110 Element Negative Strains, Tariq Moatter, Shaper Mirza, Mohammad Shahid Siddiqui, Irshad Nabi Soomro
Department of Pathology and Laboratory Medicine
Tuberculosis is still one of the most widespread infection known to mankind. Although lung is the predominant site of disease, a sizeable population in Pakistan gets intestinal disease. Clinical presentation, radiologic and endoscopic examination provide clues to the diagnosis. However, a definitive diagnosis requires biopsy material with granulomas and/or caseation complemented by acid fast staining and culture. There are many occasions when biopsy material is scanty and even in some intestinal resection cases histologic evaluation fails to confirm or rule out tuberculosis. Therefore, an investigation was conducted to assess the efficacy of PCR in the detection of mycobacterial DNA in …
The Biology Of Xenopus By R. C. Tinsley And H. C. Kobel, Rafael O. De Sá
The Biology Of Xenopus By R. C. Tinsley And H. C. Kobel, Rafael O. De Sá
Biology Faculty Publications
The Biology of Xenopus presents a summary of current knowledge about a single genus resulting from a symposium held at the Zoological Society of London in September 1992. This approach to summarizing available information has also been taken for other taxa, such as Atelopus (Lotters, 1996). However, the task of compiling data for Xenopus is enormous relative to any other amphibian group, because Xenopus laevis has become a model system for molecular and development research (Cannatella and de Sa, 1993). Unfortunately, most of our knowledge of Xenopus is biased toward this single species. There are about 20 recognized species of …
Biological Characterization Of Rev Variation In Equine Infectious Anemia Virus, Michael Belshan, Matthew E. Harris, Anne E. Shoemaker, Thomas J. Hope, Susan Carpenter
Biological Characterization Of Rev Variation In Equine Infectious Anemia Virus, Michael Belshan, Matthew E. Harris, Anne E. Shoemaker, Thomas J. Hope, Susan Carpenter
Nebraska Center for Virology: Faculty Publications
Sequence analysis identified significant variation in the second exon of equine infectious anemia virus (EIAV) rev. Functional analysis indicated that limited amino acid variation in Rev significantly altered the export activity of the protein but did not affect Rev-dependent alternative splicing. EIAV Rev can mediate export through two independent cis -acting Rev-responsive elements (RREs), and differences among Rev variants were more pronounced when both RREs were present. Variation in Rev may be an important mechanism for regulation of virus replication in vivo and may contribute to changes in clinical disease.
Confocal Microscopy: A Powerful Tool For Biological Research, Amit Singh, K. P. Gopinathan
Confocal Microscopy: A Powerful Tool For Biological Research, Amit Singh, K. P. Gopinathan
Biology Faculty Publications
Conventional light microscopy allows the observation of living as well as fixed cells and tissues to generate two-dimensional images. The out-of-focus information often obscures the ultrastructural details, especially in thick specimens with overlapping structures. The earliest available light microscopy visualized the objects in hydrated state in two-dimensions during their temporal development. The emergence of electron microscopy (EM) provided superb resolution of ultrastructural details, but it was applicable only for objects in the dehydrated state and thereby potentially introducing handling artifacts. The usefulness of optical methods, however, has been limited by the poor depth discrimination. Often, the fluorescence and reflectance images …
Adenovirus Preterminal Protein Binds To The Cad Enzyme At Active Sites Of Viral Dna Replication On The Nuclear Matrix, Peter C. Angeletti, Jeffrey A. Engler
Adenovirus Preterminal Protein Binds To The Cad Enzyme At Active Sites Of Viral Dna Replication On The Nuclear Matrix, Peter C. Angeletti, Jeffrey A. Engler
Nebraska Center for Virology: Faculty Publications
Adenovirus (Ad) replicative complexes form at discrete sites on the nuclear matrix (NM) via an interaction mediated by the precursor of the terminal protein (pTP). The identities of cellular proteins involved in these complexes have remained obscure. We present evidence that pTP binds to a multifunctional pyrimidine biosynthesis enzyme found at replication domains on the NM. Far-Western blotting identified proteins of 150 and 240 kDa that had pTP binding activity. Amino acid sequencing of the 150-kDa band revealed sequence identity to carbamyl phosphate synthetase I (CPS I) and a high degree of homology to the related trifunctional enzyme known as …
Localization Of Human Cytomegalovirus Structural Proteins To The Nuclear Matrix Of Infected Human Fibroblasts, V. Sanchez, Peter C. Angeletti, J. A. Engler, W. J. Britt
Localization Of Human Cytomegalovirus Structural Proteins To The Nuclear Matrix Of Infected Human Fibroblasts, V. Sanchez, Peter C. Angeletti, J. A. Engler, W. J. Britt
Nebraska Center for Virology: Faculty Publications
The intranuclear assembly of herpesvirus subviral particles remains an incompletely understood process. Previous studies have described the nuclear localization of capsid and tegument proteins as well as intranuclear tegumentation of capsid-like particles. The temporally and spatially regulated replication of viral DNA suggests that assembly may also be regulated by compartmentalization of structural proteins. We have investigated the intranuclear location of several structural and nonstructural proteins of human cytomegalovirus (HCMV). Tegument components including pp65 (ppUL83) and ppUL69 and capsid components including the major capsid protein (pUL86) and the small capsid protein (pUL48/49) were retained within the nuclear matrix (NM), whereas the …
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sherryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sherryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Nebraska Center for Virology: Faculty Publications
Productive replication of human immunodeficiency virus type 1 (HIV-1) in brain macrophages and microglia is a critical component of viral neuropathogenesis. However, how virus-macrophage interactions lead to neurological disease remains incompletely understood. Possibly, a differential ability of virus to replicate in brain tissue macrophages versus macrophages in other tissues underlies HIV-1 neurovirulence. To these ends, we established systems for the isolation and propagation of pure populations of human microglia and then analyzed the viral life cycles of divergent HIV-1 strains in these cells and in cultured monocytes by using identical viral inocula and indicator systems. The HIV-1 isolates included those …
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sheryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sheryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Nebraska Center for Virology: Faculty Publications
Productive replication of human immunodeficiency virus type 1 (HIV-1) in brain macrophages and microglia is a critical component of viral neuropathogenesis. However, how virus-macrophage interactions lead to neurological disease remains incompletely understood. Possibly, a differential ability of virus to replicate in brain tissue macrophages versus macrophages in other tissues underlies HIV-1 neurovirulence. To these ends, we established systems for the isolation and propagation of pure populations of human microglia and then analyzed the viral life cycles of divergent HIV-1 strains in these cells and in cultured monocytes by using identical viral inocula and indicator systems. The HIV-1 isolates included those …
Role Of The Β-Chemokine Receptors Ccr3 And Ccr5 In Human Immunodeficiency Virus Type 1 Infection Of Monocytes And Microglia, Anuja Ghorpade, Meng Qi Xia, Bradley T. Hyman, Yuri Persidsky, Adeline Nukuna, Paul Bock, Myhanh Che, Janae Limoges, Howard Gendelman, Charles R. Mackay
Role Of The Β-Chemokine Receptors Ccr3 And Ccr5 In Human Immunodeficiency Virus Type 1 Infection Of Monocytes And Microglia, Anuja Ghorpade, Meng Qi Xia, Bradley T. Hyman, Yuri Persidsky, Adeline Nukuna, Paul Bock, Myhanh Che, Janae Limoges, Howard Gendelman, Charles R. Mackay
Nebraska Center for Virology: Faculty Publications
Human immunodeficiency virus type 1 (HIV-1) infection in mononuclear phagocyte lineage cells (monocytes, macrophages, and microglia) is a critical component in the pathogenesis of viral infection. Viral replication in macrophages serves as a reservoir, a site of dissemination, and an instigator for neurological sequelae during HIV-1 disease. Recent studies demonstrated that chemokine receptors are necessary coreceptors for HIV-1 entry which determine viral tropism for different cell types. To investigate the relative contribution of the β -chemokine receptors CCR3 and CCR5 to viral infection of mononuclear phagocytes we utilized a panel of macrophage-tropic HIV-1 strains (from blood and brain tissue) to …
A Cell Cycle-Regulated Adenine Dna Methyltransferase From Caulobacter Crescentus Processively Methylates Gantc Sites On Hemimethylated Dna, Craig Stephens, Anthony J. Berdis, Irene Lee, James K. Coward, Rachel Wright, Lucy Shapiro, Stephen J. Benkovic
A Cell Cycle-Regulated Adenine Dna Methyltransferase From Caulobacter Crescentus Processively Methylates Gantc Sites On Hemimethylated Dna, Craig Stephens, Anthony J. Berdis, Irene Lee, James K. Coward, Rachel Wright, Lucy Shapiro, Stephen J. Benkovic
Biology
The kinetic properties of an adenine DNA methyltransferase involved in cell cycle regulation of Caulobacter crescentus have been elucidated by using defined unmethylated or hemimethylated DNA (DNAHM) substrates. Catalytic efficiency is significantly enhanced with a DNAHM substrate. Biphasic kinetic behavior during methyl incorporation is observed when unmethylated or DNAHM substrates are used, indicating that a step after chemistry limits enzyme turnover and is most likely the release of enzyme from methylated DNA product. The enzyme is thermally inactivated at 30 degrees C within 20 min; this process is substantially decreased in the presence of saturating concentrations of DNAHM, suggesting that …
Predicting Prognosis In Lung Cancer: Use Of Proliferation Marker, Ki67 Monoclonal Antibody, I N. Soomro, J Holmes, W F. Whimster
Predicting Prognosis In Lung Cancer: Use Of Proliferation Marker, Ki67 Monoclonal Antibody, I N. Soomro, J Holmes, W F. Whimster
Department of Pathology and Laboratory Medicine
An investigation was carried out to assess the prognostic significance of proliferation marker Ki67 in a group of lung cancer patients treated by surgery (limited disease). Tissue was not available for Ki67 immunostaining in inoperable group. The diagnosis is established by bronchial biopsy which does not carry enough tissue for frozen section and counting. This study is supplemented by estimating the prognostic significance of histological sub-types in the operable group and in a group of inoperable patients with extensive disease. These are usually treated by radiotherapy and/or chemotherapy. In all, 267 patients were studied including 105 treated by surgery. These …
Prevalence Of Broad-Host-Range Lytic Bacteriophages Of Sphaerotilus Natans, Escherichia Coli, And Pseudomonas Aeruginosa, Ellen C. Jensen, Holly S. Schrader, Brenda Rieland, Thomas L. Thompson, Kit W. Lee, Kenneth W. Nickerson, Tyler A. Kokjohn
Prevalence Of Broad-Host-Range Lytic Bacteriophages Of Sphaerotilus Natans, Escherichia Coli, And Pseudomonas Aeruginosa, Ellen C. Jensen, Holly S. Schrader, Brenda Rieland, Thomas L. Thompson, Kit W. Lee, Kenneth W. Nickerson, Tyler A. Kokjohn
Papers in Microbiology
Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natans ATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host …
Loss Of Cellular K+ Mimics Ribotoxic Stress, Mihail S. Iordanov, Bruce E. Magun
Loss Of Cellular K+ Mimics Ribotoxic Stress, Mihail S. Iordanov, Bruce E. Magun
CUP Faculty Research
The tumor promoter palytoxin has been found to activate the stress-activated protein kinase/c-Jun NH2-terminal kinase 1 (SAPK/JNK1), and it also potentiates, as demonstrated here, the p38/HOG1 mitogen-activated protein kinase and the upstream activator of SAPK/JNK1, SEK1/MKK4. In search of possible mechanisms for both the cytotoxicity and the activation of stress kinases by palytoxin, we found that palytoxin is a potent inhibitor of cellular protein synthesis. The inhibition of translation by palytoxin does not result from its direct binding to the translational apparatus. We have previously demonstrated that ribotoxic stressors (Iordanov, M. S., Pribnow, D., Magun, J. L., Dinh, …
Cytoskeletal Association Of An Esterase In Dictyostelium Discoideum, Catherine P. Chia, Lothar Bomblies, Kristy K. Taylor
Cytoskeletal Association Of An Esterase In Dictyostelium Discoideum, Catherine P. Chia, Lothar Bomblies, Kristy K. Taylor
Papers in Microbiology
A 70-kDa glycoprotein, gp70, was found enriched in the detergentinsoluble cytoskeletal fraction of axenically grown Dictyostelium discoideum cells. Its N-terminal amino acid sequence identified it as “crystal protein” (L. Bomblies et al., 1990, J. Cell Biol. 110, 669– 679). This finding was corroborated when antibody to crystal protein cross-reacted with gp70 and its deglycosylated form. The postulated esterase activity of gp70/crystal protein was verified through comparative enzyme assays of extracts derived from cells that either overexpressed or lacked gp70. Gp70 cosedimented with cytoskeletons on sucrose gradients, suggesting an interaction with the cytoskeleton. Coisolation of gp70 with detergent-extracted cells, observed by …
Fecal Coliform Transport Through Intact Soil Blocks Amended With Poultry Manure, S. W. Mcmurry, Mark S. Coyne, E. Perfect
Fecal Coliform Transport Through Intact Soil Blocks Amended With Poultry Manure, S. W. Mcmurry, Mark S. Coyne, E. Perfect
Plant and Soil Sciences Faculty Publications
Poultry production in Kentucky increased almost 200% between 1991 and 1995. Their waste is typically land applied, and fecal pathogen runoff and infiltration may cause nonpoint source groundwater pollution. We looked at the preferential flow of fecal coliforms through undisturbed soil blocks since fecal bacteria typically infiltrate the soil profile to contaminate groundwater. Poultry manure was uniformly distributed on top of sod-covered or tilled (upper 12.5 cm) soil blocks and the blocks were irrigated. Drainage was collected in 100 uniformly spaced cells beneath each block and analyzed for fecal coliform content and drainage volume. The spatial distribution of drainage and …
Cyanonews (Vol. 14, No. 1, August 1998), Jeff Elhai
Cyanonews (Vol. 14, No. 1, August 1998), Jeff Elhai
CyanoNews
CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.
Chondrocranial Morphology Of Leptodactylus Larvae (Leptodactylidae: Leptodactylinae): Its Utility In Phylogenetic Reconstruction, Peter M. Larson, Rafael O. De Sá
Chondrocranial Morphology Of Leptodactylus Larvae (Leptodactylidae: Leptodactylinae): Its Utility In Phylogenetic Reconstruction, Peter M. Larson, Rafael O. De Sá
Biology Faculty Publications
Chondrocranial morphology of leptodactylid frogs is scarcely known and has not been completely described for any species of Leptodactylus. We describe the diversity of chondrocranial morphology in the genus Leptodactylus based on the analysis of 22 species, representing the four species groups: the fuscus Group, ocellatus Group, melanonotus Group, and pentadactylus Group. Furthermore, 26 characters are identified and used in a phylogenetic analysis. The phylogenetic analysis using Physalaemus, Crossodactylus, and Hylodes as outgroups suggests two monophyletic clades within Leptodactylus: the melanonotus-ocellatus clade and the pentadactylusfuscus clade. However, it does not support the monophyly of the species groups as currently recognized …
Human Herpesvirus 8 As A Potential Sexually Transmitted Agent In Honduras, C. Sosa, W. Klaskala, B. Chandran, R. Soto, L. Sieczkowski, M. H. Wu, M. Baum, Charles Wood
Human Herpesvirus 8 As A Potential Sexually Transmitted Agent In Honduras, C. Sosa, W. Klaskala, B. Chandran, R. Soto, L. Sieczkowski, M. H. Wu, M. Baum, Charles Wood
Nebraska Center for Virology: Faculty Publications
The seroprevalence of human herpesvirus 8 (HHV-8) was studied in 326 human immunodeficiency virus (HIV)–positive and –negative persons from Honduras; women constituted 77% (n = 251) of the subjects. Sera were tested for lytic HHV-8 antibodies by an IFA, and positive samples were confirmed by a radioimmunoprecipitation assay. Of the 326 persons tested, 58 (17.8%) had HHV- 8 antibodies. Among the HIV-infected women, 22.7% were seropositive; 11.3% of the HIV-negative women were seropositive. HHV-8 seroprevalence was almost four times higher in HIV-positive female commercial sex workers (36%) than in HIV-negative female non–commercial sex workers (9.9%; odds ratio = 3.8, 95% …
Seroprevalence Of Human Herpesvirus 8 Among Zambian Women Of Childbearing Age Without Kaposi’S Sarcoma (Ks) And Mother-Child Pairs With Ks, Jun He, Ganapati Bhat, Chipepo Kankasa, Chifumbe Chintu, Charles Mitchell, Wenjing Duan, Charles Wood
Seroprevalence Of Human Herpesvirus 8 Among Zambian Women Of Childbearing Age Without Kaposi’S Sarcoma (Ks) And Mother-Child Pairs With Ks, Jun He, Ganapati Bhat, Chipepo Kankasa, Chifumbe Chintu, Charles Mitchell, Wenjing Duan, Charles Wood
Nebraska Center for Virology: Faculty Publications
The seroprevalence of human herpesvirus 8 (HHV-8) among a group of Zambian women of reproductive age and among mother-child pairs in which either one of them has Kaposi’s sarcoma (KS) was determined. A cross-sectional group of 378 pregnant women was randomly recruited into the study, and 183 (48.4%) had HHV-8 antibodies. Among the human immunodeficiency virus (HIV)-1–infected women, 51.1% were HHV-8–seropositive, whereas of HIV- 1–negative women, 47.3% were HHV-8–seropositive. In addition, 21 women index patients with KS and 5 young children index patients with KS were studied. All children with KS had mothers who were HHV-8–seropositive, while not all children …
Multiple Members Of A Third Subfamily Of P-Type Atpases Identified By Genomic Sequences And Ests, Margaret S. Halleck, Deepti Pradhan, Christie Blackman, Charlotte A. Berkes, Partrick Williamson, Robert A. Schlegel
Multiple Members Of A Third Subfamily Of P-Type Atpases Identified By Genomic Sequences And Ests, Margaret S. Halleck, Deepti Pradhan, Christie Blackman, Charlotte A. Berkes, Partrick Williamson, Robert A. Schlegel
Biology Faculty Publications
The Saccharomyces cerevisiae genome contains five P-type ATPases divergent from both of the well-known subfamilies of these membrane ion transporters. This newly recognized third subfamily can be further divided into four classes of genes with nearly equal relatedness to each other. Genes of this new subfamily are also present and expressed in multicellular organisms such as Caenorhabditis elegans and mammals; some, but not all, can be assigned to the classes identified in yeast. Different classes of genes and different genes within a class are expressed differentially in tissues of the mouse. The recently cloned gene for the mammalian aminophospholipid translocase …