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Articles 1 - 9 of 9
Full-Text Articles in Life Sciences
High-Throughput Nanoliter Dispensing Device For Biological Applications, Cole Reynolds, Euiwon Bae Dr., J Paul Robinson Dr.
High-Throughput Nanoliter Dispensing Device For Biological Applications, Cole Reynolds, Euiwon Bae Dr., J Paul Robinson Dr.
The Summer Undergraduate Research Fellowship (SURF) Symposium
Pathogen identification is a field that can contribute largely to the prevention of the spreading of illness and disease. In the past, pathogen identification has been a long and arduous process due to the time-consuming processes and steps that requires technician’s time and effort. With new technologies emerging however, screening of bacteria colonies can be done in a quick and high-throughput way. The problem is that using the current methods, bacteria cannot be transferred to petri dishes fast enough to keep up with the new screening methods. The current study focuses on exploring different methods to create an ergonomic device …
Improving Biomanufacturing Production With Novel Elp-Based Transcriptional Regulators, Juya Jeon, Logan R. Readnour, Kevin V. Solomon
Improving Biomanufacturing Production With Novel Elp-Based Transcriptional Regulators, Juya Jeon, Logan R. Readnour, Kevin V. Solomon
The Summer Undergraduate Research Fellowship (SURF) Symposium
Microbes can be used to produce valuable drugs, chemicals, and biofuels, but their potential has not been fully realized due to low production yields. To improve biomanufacturing processes and yield, we are developing novel, transcriptional regulators using biosynthesis technology in order to improve cellular health and overall production. Our regulator contains elastin-like polypeptides (ELPs), which make ideal sensors since they exhibit a sharp, inverse phase transition to indicators of cell health such as intracellular pH and ionic strength, and external stimuli such as temperature. We hypothesize that ELP can be fused to transcription factors to control expression of target genes. …
Effect Of Carbohydrates On The Gut Microbiome, Maciej Filar, Mohit S. Verma
Effect Of Carbohydrates On The Gut Microbiome, Maciej Filar, Mohit S. Verma
The Summer Undergraduate Research Fellowship (SURF) Symposium
The microbiome within the gut is directly linked to biological processes within a person, influencing factors such as metabolism, signaling pathways, and available nutrients. Long term dieting is known to alter ecological conditions within the gut, allowing certain types of microbes to flourish. Therefore, the overall health of an individual is ultimately influenced by shifts in the microbial community state caused by persistent dieting. This study investigates the connection between diet and the microbiome and draws an understanding of how common carbohydrates in food can affect bacterial composition. Using KBase software, anaerobic bacterial growth was investigated for bacteria subject to …
Finding Human Proteins That Bind To A Lassa Virus Protein, Maria Alejandra Pardo Ruge, Veronica J. Heintz, Douglas J. Lacount
Finding Human Proteins That Bind To A Lassa Virus Protein, Maria Alejandra Pardo Ruge, Veronica J. Heintz, Douglas J. Lacount
The Summer Undergraduate Research Fellowship (SURF) Symposium
Viral hemorrhagic fevers are severe illnesses caused by many different viruses. Lassa Virus is one of these important pathogens in Western Africa, causing hemorrhagic fever and eventually death without early medical treatment. There is no vaccine and there is little information on host-pathogen interactions. Therefore, the interaction between viral proteins and host targets is useful to understand Lassa virus’s lifecycle and pathology, and to develop ways to prevent infection. In this project, we study the nucleoprotein of Lassa virus (NP), which has been reported to have anti-interferon (IFN) activity through elimination of double stranded RNA (dsRNA). These features could be …
Secreted Ns1 Aids In Dengue Virus Entry By Binding Heparan Sulfate, Morgan E. Schafer, Michael Dibiasio-White, Richard J. Kuhn
Secreted Ns1 Aids In Dengue Virus Entry By Binding Heparan Sulfate, Morgan E. Schafer, Michael Dibiasio-White, Richard J. Kuhn
The Summer Undergraduate Research Fellowship (SURF) Symposium
A large portion of the world’s population lives in an area where they are at risk of contracting Dengue Virus (DENV). While infection with DENV can result in a wide range of clinical manifestations, from flu-like symptoms to hemorrhagic shock, vascular leakage, and even death, no treatments currently exist. Previous studies have shown that higher levels of viral non-structural protein 1 (NS1) in the blood of infected patients correlate with more severe disease. NS1 has been shown to play many roles in the viral lifecycle, but the mechanisms by which NS1 executes these functions are unknown. Preliminary data from our …
A Compact System For Photon Counting Based On Silicon Photomultiplier, Youngwoo Cho, Youngkee Jung, Euiwon Bae
A Compact System For Photon Counting Based On Silicon Photomultiplier, Youngwoo Cho, Youngkee Jung, Euiwon Bae
The Summer Undergraduate Research Fellowship (SURF) Symposium
A compact and portable detection system is necessary to measure the amount of pollutant from environmental sample by detecting and quantifying the light emitted by bioluminescent reporters. This silicon photomultiplier based project is hoping to acquire even more accurate data at a far lower light level than previously developed smartphone based system. After pre-amplification and comparator, the signal is separated from the internal noise present in the overall circuit. Next, the microcontroller counts the number of pulses generated by the comparator in a set amount of time and transfers the data to the Bluetooth module for the smartphone to receive …
You Don’T Have To Go Home But You Carrot Stay Here: Root-Knot Nematode Biological Control In Carrots, Casey R. Martin, Sahar Abdelrazek, Lori A. Hoagland
You Don’T Have To Go Home But You Carrot Stay Here: Root-Knot Nematode Biological Control In Carrots, Casey R. Martin, Sahar Abdelrazek, Lori A. Hoagland
The Summer Undergraduate Research Fellowship (SURF) Symposium
Root-knot nematodes cause tens of billions of dollars in damages annually in agricultural production. The enormous economic losses brought about by nematodes, combined with the downsides to current methods for handling them, have led to an increased focus in understanding natural plant defense mechanisms and finding alternative methods to lessen the damage done by root-knot nematodes. Endophyte biocontrol agents such as Trichoderma harzianum have shown promise in reducing nematode damage. Recent studies have demonstrated that soil management practices and plant genotype can influence endophyte community structure, but it is unclear whether such differences influence susceptibility to nematode damage. This study …
A Novel Synthetic Yeast For Enzymatic Biodigester Pretreatment, Tianyu Tan, Mark S. Aronson, Arren Liu, Jill H. Osterhus, Melissa Robins, Suraj Mohan, Erich Leazer, Bowman Clark, Alexa Petrucciani, Katherine Lowery, James Welch, Casey Martin, Helena Lysandrou, Michael E. Scharf, Jenna Rickus
A Novel Synthetic Yeast For Enzymatic Biodigester Pretreatment, Tianyu Tan, Mark S. Aronson, Arren Liu, Jill H. Osterhus, Melissa Robins, Suraj Mohan, Erich Leazer, Bowman Clark, Alexa Petrucciani, Katherine Lowery, James Welch, Casey Martin, Helena Lysandrou, Michael E. Scharf, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Lignin, a complex organic polymer, is a major roadblock to the efficiency of biofuel conversion as it both physically blocks carbohydrate substrates and poisons biomass degrading enzymes, even if broken down to monomer units. A pretreatment process is often applied to separate the lignin from biomass prior to biofuel conversion. However, contemporary methods of pretreatment require large amounts of energy, which may be economically uncompelling or unfeasible. Taking inspiration from several genes that have been isolated from termites and fungi which translate to enzymes that degrade lignin, we want to establish a novel “enzymatic pretreatment” system where microbes secrete these …
Study Of Coronavirus Protease Using Cfp-Yfp Fluorescent Assay, Caitlin E. Specht, Andrew Mesecar Ph.D., Katrina Molland Ph.D.
Study Of Coronavirus Protease Using Cfp-Yfp Fluorescent Assay, Caitlin E. Specht, Andrew Mesecar Ph.D., Katrina Molland Ph.D.
The Summer Undergraduate Research Fellowship (SURF) Symposium
Middle Eastern Respiratory Syndrome (MERS) is an emerging viral disease originating in the Arabian Peninsula with a current mortality rate of nearly fifty percent throughout Europe and Asia according to the World Health Organization. Characterization of this disease is being done to understand the basis of viral replication. One target for viral inhibition are replication proteases. Replication proteases are enzymes that cleave proteins specific to cell growth and reproduction that form the viral replicase complex making them an ideal target for viral replication inhibition. First, replication proteases were characterized using a fluorescence resonance energy transfer (FRET) construct by measuring the …