Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Prevalence Of Broad-Host-Range Lytic Bacteriophages Of Sphaerotilus Natans, Escherichia Coli, And Pseudomonas Aeruginosa, Ellen C. Jensen, Holly S. Schrader, Brenda Rieland, Thomas L. Thompson, Kit W. Lee, Kenneth W. Nickerson, Tyler A. Kokjohn
Prevalence Of Broad-Host-Range Lytic Bacteriophages Of Sphaerotilus Natans, Escherichia Coli, And Pseudomonas Aeruginosa, Ellen C. Jensen, Holly S. Schrader, Brenda Rieland, Thomas L. Thompson, Kit W. Lee, Kenneth W. Nickerson, Tyler A. Kokjohn
Papers in Microbiology
Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natans ATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host …
Cytoskeletal Association Of An Esterase In Dictyostelium Discoideum, Catherine P. Chia, Lothar Bomblies, Kristy K. Taylor
Cytoskeletal Association Of An Esterase In Dictyostelium Discoideum, Catherine P. Chia, Lothar Bomblies, Kristy K. Taylor
Papers in Microbiology
A 70-kDa glycoprotein, gp70, was found enriched in the detergentinsoluble cytoskeletal fraction of axenically grown Dictyostelium discoideum cells. Its N-terminal amino acid sequence identified it as “crystal protein” (L. Bomblies et al., 1990, J. Cell Biol. 110, 669– 679). This finding was corroborated when antibody to crystal protein cross-reacted with gp70 and its deglycosylated form. The postulated esterase activity of gp70/crystal protein was verified through comparative enzyme assays of extracts derived from cells that either overexpressed or lacked gp70. Gp70 cosedimented with cytoskeletons on sucrose gradients, suggesting an interaction with the cytoskeleton. Coisolation of gp70 with detergent-extracted cells, observed by …