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The Effects Of Lotion Fatty Acids On Gram Negative Skin Flora: Implications For Membrane Remodeling And Pathogenicity, Adrianna Eder
The Effects Of Lotion Fatty Acids On Gram Negative Skin Flora: Implications For Membrane Remodeling And Pathogenicity, Adrianna Eder
Honors Theses
AIM: The purpose of this experiment was to determine whether or not Enterobacter aerogenes, Acinetobacter baumannii, and Klebsiella pneumoniae, which are three gram negative bacteria commonly found on the skin, utilize fatty acids in a manner that affects bacterial phenotype. Furthermore, an objective was to gain a deeper understanding of how the alteration of bacterial membranes, as a result of fatty acid uptake, affected the pathogenicity, membrane permeability, and resistance in gram negative bacteria. METHODS: The lipids were extracted using the method of Bligh and Dyer and analyzed using thin-layer chromatography (TLC). A series of tests were performed, including a …