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The Effects Of Lotion Fatty Acids On Gram Negative Skin Flora: Implications For Membrane Remodeling And Pathogenicity, Adrianna Eder
The Effects Of Lotion Fatty Acids On Gram Negative Skin Flora: Implications For Membrane Remodeling And Pathogenicity, Adrianna Eder
Honors Theses
AIM: The purpose of this experiment was to determine whether or not Enterobacter aerogenes, Acinetobacter baumannii, and Klebsiella pneumoniae, which are three gram negative bacteria commonly found on the skin, utilize fatty acids in a manner that affects bacterial phenotype. Furthermore, an objective was to gain a deeper understanding of how the alteration of bacterial membranes, as a result of fatty acid uptake, affected the pathogenicity, membrane permeability, and resistance in gram negative bacteria. METHODS: The lipids were extracted using the method of Bligh and Dyer and analyzed using thin-layer chromatography (TLC). A series of tests were performed, including a …
The Uptake And Incorporation Of Exogenous Fatty Acids Confer Phenotypic Advantages To Acinetobacter Baumannii, Saba A. Munir
The Uptake And Incorporation Of Exogenous Fatty Acids Confer Phenotypic Advantages To Acinetobacter Baumannii, Saba A. Munir
Honors Theses
AIM: Acinetobacter baumannii is a ubiquitous gram-negative bacterium found on a variety of surfaces that include skin, hair, and soil. Some gram-negative bacteria like A. baumannii have the ability to acquire and incorporate fatty acids into their phospholipid membranes. Known as ‘Iraqibacter,’ A. baumannii has emerged as a significant cause of nosocomial infections in the United States and abroad. This study was designed to determine whether structural alterations occur in A. baumannii upon exposure to a wide range of polyunsaturated fatty acids (PUFAs) as well as the significance of this phenomenon in terms of survival and resistance to stress. Thin …
Identification Of Bacterial Species Using Colony Pcr, Kaiti Walker
Identification Of Bacterial Species Using Colony Pcr, Kaiti Walker
Honors Theses
My research consisted of two separate parts, both involving the PCR methods. In part one I was trying to identify bacteria isolated from a chicken coop. In part two I was trying to identify bacteria isolated from the bluff. In both parts, I used a technique called colony PCR. This concept uses a single colony of bacteria to complete the PCR reaction. One pipet tip from a single colony is selected from the plate and mixed into a tube containing ultrapure water. A small amount of this bacterial mixture is then added into the PCR reaction. In the first part …