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Full-Text Articles in Life Sciences
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic …
Molecular Types And Antimicrobial Resistance Profile Of Staphylococcus Aureus Isolated From Dairy Cows And Farm Environments, Dattatray Gandhale, Rahul Kolhe, Saba Nalband, Padmakar Deshpande, Urmila Jagtap, Chandrakant Dhandore, Sujata Bhave, Sameer Jadhav, Dushyant Muglikar, Smita Kolhe
Molecular Types And Antimicrobial Resistance Profile Of Staphylococcus Aureus Isolated From Dairy Cows And Farm Environments, Dattatray Gandhale, Rahul Kolhe, Saba Nalband, Padmakar Deshpande, Urmila Jagtap, Chandrakant Dhandore, Sujata Bhave, Sameer Jadhav, Dushyant Muglikar, Smita Kolhe
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to assess the prevalence of Staphylococcus aureus in cows with subclinical mastitis and detect enterotoxin genes and patterns of antimicrobial resistance. Out of 180 animals, subclinical mastitis was detected in 78 cows (46.99%) and three buffalo (21.43%). The prevalence of clinical mastitis was low (3.61%). S. aureus could be isolated from mastitic milk (67/81), udder swabs (38/81), floor swabs (13/27), milking machine liner (5/8), and healthy animals- milk (12/22). All the strains were confirmed by PCR targeting amplification of the femA gene. Out of 52 PCR-confirmed S. aureus samples, 17 were enterotoxin-producing strains. The …