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Full-Text Articles in Life Sciences
Determination Of Antibiotic Resistance In Salmonella Typhimurium And Salmonella Kentucky Serotypes Of Animal Origin Using Conventional And Molecular Methods, Zeynep Şik, Mehmet Akan
Determination Of Antibiotic Resistance In Salmonella Typhimurium And Salmonella Kentucky Serotypes Of Animal Origin Using Conventional And Molecular Methods, Zeynep Şik, Mehmet Akan
Turkish Journal of Veterinary & Animal Sciences
The high incidence of multidrug-resistant Salmonella Typhimurium and Salmonella Kentucky isolates is a concern for human and animal health. This study aimed to investigate the antibiotic resistance determinants of a total of 150 S. Typhimurium and S. Kentucky isolates obtained from cows, calves, lambs, and poultry. Salmonella isolates were tested against 13 different antimicrobials using the Kirby-Bauer disc diffusion method, and genotypic antimicrobial resistance determinants were investigated by polymerase chain reaction. Significant differences were detected among serovars for gentamicin, streptomycin, ampicillin, sulfonamide, nalidixic acid, ciprofloxacin, and tetracycline resistance, with the rates of resistance to these antibiotics being determined to be …
Comparison Of Bacterial Profile And Antibiotic Susceptibility Isolated From Surgical Site After Ventral Midline And Lateral Flank Approaches Of Ovariohysterectomy In Queens And Bitches, Ebru Karakaya Bi̇len, Serpi̇l Kahya Demi̇rbi̇lek, Öznur Yilmaz, Muhammet Ali̇ Karadağ, Havva Aner
Comparison Of Bacterial Profile And Antibiotic Susceptibility Isolated From Surgical Site After Ventral Midline And Lateral Flank Approaches Of Ovariohysterectomy In Queens And Bitches, Ebru Karakaya Bi̇len, Serpi̇l Kahya Demi̇rbi̇lek, Öznur Yilmaz, Muhammet Ali̇ Karadağ, Havva Aner
Turkish Journal of Veterinary & Animal Sciences
The aim of the study was to identify the bacterial profile and antimicrobial susceptibility from surgical site cultures in queens and bitches undergoing ovariohysterectomy via ventral midline and lateral flank approaches. Healthy 22 queens and 21 bitches were assigned randomly either ventral midline or lateral flank approach for routine ovariohysterectomy. Surgical site samples were collected before the surgery and on the 1$^{st}$, 2$^{nd}$ , and 3 $^{rd}$days after the surgery for microbiological analysis. A total of 70 different strains were isolated from 50 (29%) of 172 samples. In both queens and bitches the total number of bacteria isolated from the …
Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan
Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan
Turkish Journal of Veterinary & Animal Sciences
Salmonellosis caused by Salmonella agents is the second most common zoonotic infection in humans. In recent years, Salmonella's increasing antimicrobial resistance (AMR) has been a concern. The major transmission route of Salmonella is consumption of contaminated poultry products. Therefore, monitoring of antimicrobial resistance in chicken-originated Salmonella is critically important. This study investigated AMR in four commonly isolated Salmonella serovars from chickens, namely Salmonella Enteritidis (S. Enteritidis), Salmonella Infantis (S. Infantis), Salmonella Kentucky (S. Kentucky), and Salmonella Typhimurium (S. Typhimurium). A total of 133 isolates were examined by phenotypic and genotypic AMR characterization. Resistance to 14 different antimicrobials and eight …
Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil
Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil
Turkish Journal of Veterinary & Animal Sciences
Antimicrobial agents are used for suppressing some bacterial infections during the rearing of poultry. In this study; the antibiotic resistance properties of Extended Spectrum ßeta-lactamase (ESßL), Amp-C, carbapenemase-producing Escherichia coli (E. coli) strains isolated from chicken breast meat were determined by disc diffusion and E-test methods. Additionally, it is aimed to determine antibiotic resistance genes by classical polymerase chain reaction (PCR). In this context, E. coli strains were isolated from 7 of 100 chicken breast meat (fillets) and identified by a rapid identification system (BBL Crystal). Disc diffusion test results of the isolates were evaluated and 100% to tetracycline; ampicillin, …
In Vitro Antibiotic Resistance Of Staphylococci Isolated From Different Animal Species, Nurdan Karacan Sever, Mehmet Akan
In Vitro Antibiotic Resistance Of Staphylococci Isolated From Different Animal Species, Nurdan Karacan Sever, Mehmet Akan
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study is to investigate resistance to antibiotics of Staphylococcus species isolated from various samples belonging to different animal species. Among 48 Staphylococcus spp. strains, Staphylococcus intermedius was the most common species, followed by S. aureus, S. epidermidis, S. hyicus, S. saprophyticus. In a total of 48 Staphylococcus strains, the highest antibiotic resistance was observed to oxacillin (79.17%), tetracycline (39.58%), and ampicillin and cefoxitin (31.25%). Of 48 Staphylococcus strains, 42 showed resistance to at least one antimicrobial agent, while 23 of the strains had multidrug resistance. Antimicrobial resistance to tetracycline and ampicillin, erythromycin, streptomycin was detected frequently …
The Determination Of Antimicrobial And Antibiofilm Activities Of Foodborne Lactic Acid Bacteria Against Enterobacter Cloacae Isolates, Beytullah Kenar, Mi̇ne Eri̇k, Sevi̇m Feyza Erdoğmuş, Safi̇ye Eli̇f Korcan, Zahi̇de Köse, Gül Durmaz
The Determination Of Antimicrobial And Antibiofilm Activities Of Foodborne Lactic Acid Bacteria Against Enterobacter Cloacae Isolates, Beytullah Kenar, Mi̇ne Eri̇k, Sevi̇m Feyza Erdoğmuş, Safi̇ye Eli̇f Korcan, Zahi̇de Köse, Gül Durmaz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the antimicrobial and antibiofilm activities of 10 different lactic acid bacteria (LAB) strains isolated from local food sources of animal origin against 4 Enterobacter cloacae isolates obtained from clinical cases and determine their adhesion potentials to intestinal epithelial cells. In this study, all Enterobacter cloacae isolates (P3, P4, P5, P7) identified with the BD Phoenix automation system were detected to form biofilm with both Congo red agar and Microtiter plate methods. Amoxicillinclavulanate, cefuroxime, and ampicillin resistance was determined in all isolates. It was determined that LAB strains producing exopolysaccharide (EPS) were able …
Phylogrouping And Antimicrobial Resistance Analysis Of Extraintestinal Pathogenic Escherichia Coli Isolated From Poultry Species, Sujata Bhave, Rahul Kolhe, Ranjan Mahadevaswamy, Chandrakant Bhong, Samir Jadhav, Saba Nalband, Dattatray Gandhale, Dushyant Muglikar
Phylogrouping And Antimicrobial Resistance Analysis Of Extraintestinal Pathogenic Escherichia Coli Isolated From Poultry Species, Sujata Bhave, Rahul Kolhe, Ranjan Mahadevaswamy, Chandrakant Bhong, Samir Jadhav, Saba Nalband, Dattatray Gandhale, Dushyant Muglikar
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to determine the prevalence and antimicrobial resistance pattern of extraintestinal pathogenic Escherichia coli (ExPEC) in the feces of healthy poultry and retail chicken. All 146 E. coli recovered from 351 samples were screened by PCR for detection of ExPEC strains. Nineteen (13.01%) isolates were confirmed as ExPEC. Distribution of ExPEC strains was revealed as follows: broiler (25%), layer (15.87%), and raw chicken (3.12%). Turkey, duck, and water samples were negative for ExPEC strains. ExPEC strains belonged to phylogenetic groups B2 (52.63%), A (36.84%), and D (10.53%). Twenty-three (15.75%) isolates were ESBL-positive, including four ExPEC strains. ESBL-positive …
Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer
Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer
Turkish Journal of Veterinary & Animal Sciences
Innumerable foodborne pathogens including Salmonella and Escherichia coli pose a serious threat to human health and food safety. As perishable foods, poultry products are considered one of the most common sources of foodborne pathogens including Salmonella and E. coli due to transmission of drug resistance, dissemination of organisms, and cross-contamination. In our study, phenotypic and genotypic characterizations of Salmonella enterica and E. coli isolated from packaged raw chicken products were carried out. Samples belonging to different commercial brands were collected in Ankara in 2015. Among 15 out of 19 Salmonella enterica subsp. enterica strains isolated from different and/or same poultry …
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic …
Molecular Types And Antimicrobial Resistance Profile Of Staphylococcus Aureus Isolated From Dairy Cows And Farm Environments, Dattatray Gandhale, Rahul Kolhe, Saba Nalband, Padmakar Deshpande, Urmila Jagtap, Chandrakant Dhandore, Sujata Bhave, Sameer Jadhav, Dushyant Muglikar, Smita Kolhe
Molecular Types And Antimicrobial Resistance Profile Of Staphylococcus Aureus Isolated From Dairy Cows And Farm Environments, Dattatray Gandhale, Rahul Kolhe, Saba Nalband, Padmakar Deshpande, Urmila Jagtap, Chandrakant Dhandore, Sujata Bhave, Sameer Jadhav, Dushyant Muglikar, Smita Kolhe
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to assess the prevalence of Staphylococcus aureus in cows with subclinical mastitis and detect enterotoxin genes and patterns of antimicrobial resistance. Out of 180 animals, subclinical mastitis was detected in 78 cows (46.99%) and three buffalo (21.43%). The prevalence of clinical mastitis was low (3.61%). S. aureus could be isolated from mastitic milk (67/81), udder swabs (38/81), floor swabs (13/27), milking machine liner (5/8), and healthy animals- milk (12/22). All the strains were confirmed by PCR targeting amplification of the femA gene. Out of 52 PCR-confirmed S. aureus samples, 17 were enterotoxin-producing strains. The …
Prevalence And Patterns Of Antimicrobial Resistant Of Fecal Escherichia Coli Isolates Among Pigs On Eight Farrow-To-Finish Farms And Their Production Environments In Shandong Province, China, Hongchao Zhou, Lin Liu, Zhaofang Tian, Wenjuan Zhang
Prevalence And Patterns Of Antimicrobial Resistant Of Fecal Escherichia Coli Isolates Among Pigs On Eight Farrow-To-Finish Farms And Their Production Environments In Shandong Province, China, Hongchao Zhou, Lin Liu, Zhaofang Tian, Wenjuan Zhang
Turkish Journal of Veterinary & Animal Sciences
The main objectives of this study were to determine the prevalence and antimicrobial resistance patterns of Escherichia coli isolates in feces of pigs and their production environments on farms. A total of 560 isolates of Escherichia coli, from 112 samples obtained from weaner and finisher pigs, feed, water, and house sparrows on 8 farrow-to-finish farms, were tested for susceptibility to 16 antimicrobials. The prevalence of resistance varied widely (0.0% to 69.5%) among the antimicrobials tested. From the tested swines, 75.75% of the isolates were resistant to one or more antimicrobials. Resistance was significantly more frequent (P = 0.005, P < 0.01) in swine isolates compared to those from feed, water, and sparrows and significantly more frequent (P = 0.007, P < 0.01) in weaner pigs compared to finisher pigs. The findings indicate that resistance to a broad range of antimicrobials was prevalent among fecal E. coli isolates of swine on the study farms. Fortunately, E. coli was not isolated from the feed, water, and house sparrows.