Life Sciences Commons^™

Petroleum-Utilizing Bacteria: The Biological Line Defense Against Oil Pollution, Steven W. Sokolowski

Biological Sciences Theses & Dissertations

A silica gel-petroleum (SGP) medium was developed which is reliable, approximately the same cost as that of the corresponding agar-based petroleum medium, and can be made sufficiently firm to use in streaking or the spread-plate technique. Advantages of the medium include: the incorporation of all of the major ions of seawater in accordance with Dittmar's Law (the salinity of which can easily be varied from 0 to 35 parts per thousand); a wide workable pH range (5.5 to 11.6); negligable pH drift(~ 0.2 pH units after 166 days); the elimination of all but negligable amounts of syneresis, the ability to …

Enzyme Linked Immunosorbent Assay For Anatid Herpesvirus, Margaret Anne Lewis

Biological Sciences Theses & Dissertations

Duck plague virus causes a hemorrhagic disease affecting the family Anatidae, resulting in 60-80% mortality in captive flocks. Plaque and neutralization assays for Anatid herpesvirus (AHV) require 5-10 days for completion and are restricted by the seasonal availability of fertile duck eggs. The enzyme linked innnunosorbent assay (ELISA) is rapid and specific and avoids the use of living cells. The Holland strain of AHV was grown on duck embryo fibroblasts and purified by banding on CsCl gradients. The resulting virus was used to raise antisera in prebled New Zealand white male rabbits. Antibody production was confirmed in double diffusion plates …

In Vitro Enzymatic Assay Of Rna Methylation, Pamela Jean Eubanks Gallup

Chemistry & Biochemistry Theses & Dissertations

An assay procedure for in vitro enzymatic methylation of mannnalian ribosomal RNA has been developed in this study. The assay procedure, utilized for the comparison of normal and neoplastic methylase activities (using mouse liver and Ehrlich ascites cells as sources of enzyme), is a modification of previously published methods (52,53). A 100,000 x g supernatant (SlOO) enzyme preparation was incubated with 28S-5.8S rRNA and tritium-labeled S-adenosyl-L-methionine. The RNA was extracted, applied to DEAE cellulose paper, washed, and the radioactivity counted. The neoplastic cell methylase preparation was more active in methylating both exogenous neoplastic and normal 28S-5.8S rRNA than the normal …

Surgical Management Of Multicentricity, Nipple-Areola Involvement, And Bilaterality In Breast Carcinoma, F. Samhouri, M. Block, A. Kambouris

Henry Ford Hospital Medical Journal

During a five-year period, 457 women who underwent partial, segmental, or total mastectomy were assessed for multicentricity (31%), nipple-areola involvement (17%), and bilateral breast carcinoma (9%). These factors determined the extent of surgery performed and the possibilities for later breast reconstruction. The frequency of nipple-areola involvement in breast cancer strongly indicates the need for alternatives to their preservation for breast reconstruction. The study concludes that total mastectomy for invasive carcinoma is the preferred method of treatment for early breast cancer until further clinical trials have assessed the value of segmentectomy.

Henry Ford Hospital: Options And Opportunities In The 1980s, Douglas S. Peters, Bruce W. Steinhauer

Henry Ford Hospital Medical Journal

This presentation by Mr. Peters and Dr. Steinhauer was given before the Henry Ford Hospital Professional Staff at a semi-annual dinner and staff meeting held on Tuesday, May 6, 1980. It offers a timely assessment of the position and prospects of the Hospital as we enter the new decade. On occasion, the journal features nontechnical articles like this presentation that we believe are of general interest to our readers.

Combining Provocative Agents Of Calcitonin To Detect Medullary Carcinoma Of The Thyroid, J. C. Sisson, M. D. Gross, J. E. Frietas, C. E. Jackson, B. G. England

Henry Ford Hospital Medical Journal

The presence of clinically undetectable medullary thyroid cancer can be revealed by characterisdc patterns of calcitonin in serum after the injection of a provocative agent, pentagastrin or calcium ion. However, in some reports, medullary thyroid cancer was uncovered in a test with pentagastrin but not in one with calcium ion, and vice versa. To reduce the number of erroneous responses, each provocative agent has been employed in separate tests. Combining pentagastrin and calcium into one stimulus should give the advantage of each agent while requiring less time and fewer analyses in a single test. We combined pentagastrin injection and calcium …

Acute And Massive Intraparenchymal Hemorrhages Of The Posterior Fossa, Dennis E. Wilkins

Henry Ford Hospital Medical Journal

Acute, massive cerebellar and pontine hemorrhages are discussed in the context of the routine availability of cranial computerized tomography. The diagnosis of these disorders is emphasized. An updated clinical review of the edologic, pathogenetic, and therapeutic aspects is also presented, with particular attention to the importance of hypertensive cerebrovascular disease as the most common underlying disorder.

Preliminary Experience With Endoscopic Papillotomy, Bernard Schuman

Henry Ford Hospital Medical Journal

Endoscopic papillotomy is a non-operadve technique used to manage choledocholithiasis and papillary stenosis. Considerable experience in Europe and initial results in a few medical centers in the United States indicate that it is effective and safe. Six papillotomy procedures were performed at Henry Ford Hospital without complication, and with a successful outcome in five. Endoscopic papillotomy is the procedure of choice for retained common bile duct stones less than 2 cm in diameter.

1980 Publications Of The Staff Of Henry Ford Hospital

Henry Ford Hospital Medical Journal

No abstract provided.

Prenatal Diagnosis Of Fetal Cystic Hygromas Associated With Generalized Lymphangiectasis, Chang Y. Lee, Beatrice L. Madrazo, Daniel L. Van Dyke, Jewel Smith

Henry Ford Hospital Medical Journal

Ultrasonography has made possible the prenatal diagnosis of many congenital fetal abnormalities. This report describes two cases of bilateral cystic hygromas of the neck associated with generalized lymphangiectasis that were diagnosed by ultrasound. Ultrasonic scans revealed moderate polyhydramnios: thick, edematous placenta and edematous fetus with large cystic mass occupying both sides of the neck and extending to the upper chest wall, ascites, and pleural effusion at gestational ages of 21.5 and 24 weeks, respectively. In one case, chromosomal study from amniotic fluid cell culture revealed X chromosome monosomy, often associated with lymphatic anomalies. The prenatal diagnosis was confirmed at birth: …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

The Role Of The Parotid Salivary Glands In The Regulation Of Insulin Secretion, Kenneth L. Millard

Loma Linda University Electronic Theses, Dissertations & Projects

Stress of handling, partial or complete sialoadenectomy, and the administration of parotid hormone (PH), sodium fluoride (NaF), or guanidino-succinic acid (GSA) were examined for effects on fasting plasma glucose and insulin or effects on intravenous glucose tolerance in male Sprague-Dawley rats. Basal glucose and insulin were substantially elevated as a result of the stress of translocation in large animals, but were not altered in small animals. A rise in plasma insulin occured in control animals 20 minutes following anesthesia and the removal of a blood sample by cardiac puncture but not in animals that had been handled twice daily for …

The Effect Of Exercise On Blood Flow In Pregnant Guinea Pigs, Marjorie Correll Sladek

Loma Linda University Electronic Theses, Dissertations & Projects

Recent investigations have shown decreased fetal weight in pregnant animals who were exercised regularly during pregnancy (Gilbert; Nelson; Longo, 1979; Terada, 1974). The purpose of this experimental study was to investigate fetal weight gain and measure blood flow in pregnant guinea pigs who were exercised 45 minutes per day; and thus answer the hypothesis that prolonged maternal exercise will cause decreased weight gain and decreased placental blood flow during pregnancy.

Twenty pregnant guinea pigs were exercised 15 minutes three times daily from 10 days gestation to approximately 60-62 days gestation (67 days being term) and were compared with 19 control …

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Second-Year Follow-Up Study On Systematic Treatment Of Chronic Pain With Antidepressants, Dietrich Blumer, Mary Heilbronn

Henry Ford Hospital Medical Journal

Systematic therapy with antidepressant drugs can be an effective treatment for chronic pain patients with the pain-prone disorder. This survey follows the pain status, medications, depressive symptoms, work status, and treatment of 104 patients treated for chronic pain at Henry Ford Hospital's Pain Clinic during a two-year period for chronic pain in the absence of a significant somatic disorder. No patients were excluded from treatment and the dropout rate of 64% after two years was not surprising. The pain of those who complied with therapy improved significantly (62%), compared to that of the patients who had dropped out of the …

The Epidural Defect On Myelography: Some Unusual Causes, Keenan L. Carroll

Henry Ford Hospital Medical Journal

This report describes four cases of epidural defect found on myelography that illustrate some unusual causes of this defect. These include a type I chondrosarcoma, primary hemangioendothelioma, hematoma, and an epidural abscess. Surgery was performed, and all four patients have recovered with little or no remaining neurological deficit. If there is anything unusual about the epidural defect or if the clinical findings do not agree with the radiographic films, the radiologist should be alert for other, less common causes of the defect.

Dysfunction Of Mitral Ball Valve Prosthesis, M. R. S. Nair, Wolf F. C. Duvernoy, Daniel T. Anbe, Eldred G. Zobl, Agustin Arbulu

Henry Ford Hospital Medical Journal

Three case histories of patients with malfunctions of Smeloff-Cutter mitral ball valve prostheses are presented here - one underwent successful replacement. The clinical diagnosis was made by observing a marked variation in the A2OC interval and intermittent absence of the opening click. Phonocardiograms were diagnostic in all three cases. while echocardiograms and even angiograms did not uniformly diagnose prosthetic valve dysfunction. Periodic phonocardiographic evaluations may be helpful in early detection of the prosthetic valve dysfunction. Once the diagnosis is established, immediate surgical treatment is imperative to prevent sudden death.

The Effect Of Folic Acid Supplementation On Gingival Healing After Oral Hygiene Therapy, Marilyn J. Wankier

Loma Linda University Electronic Theses, Dissertations & Projects

To determine the effect of folic acid supplementation on gingival healing after oral hygiene therapy, eight teeth (#3, 4, 5, 6, 11, 12, 13, 14) of sixteen human subjects with moderate levels of gingival irritation were studied. Clinical parameters included measurement of gingival fluid, pocket depth, bleeding upon probing, and plaque. Serum and red blood cell folic acid levels were determined using radioimmunoassay (RIA). Based on clinical parameters and serum folate levels, two similarly matched groups were established. The teeth were scaled and polished. Brushing and flossing instructions were given.

After one week of oral hygiene therapy alone, the experimental …

The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross

Dartmouth Scholarship

We have developed a procedure for selectively enriching a mRNA population for inducible sequences. Other than the induced mRNA species, the population of mRNA in control cells is approximately the same as the mRNA population in induced cells. Cytoplasmic mRNA from control cells is bound to oligo (dT)-cellulose and used as a template for reverse transcriptase, the oligo (dT) serving as a primer. After removing the template mRNAs, the cDNA-cellulose column is used to hybridize a population of mRNAs from induced cells. The non-hybridized poly A ⁺ RNAs are greatly enriched in the inducible sequences. We have used this technique …

Fight For Alternatives Gathers Momentum: Commitments To Help Lab Animals Are Extracted From Industry And Government

Close Up Reports

The Humane Society of the United States wants to see an eventual end to all pain and suffering inflicted on animals in the laboratory. We are working hard to make this happen, and the "alternatives" concept is basic to our program. We want scientists to find alternative methods of testing and experimentation that do not involve the use of animals.

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Clinical Thermoradiotherapy, H. I. Bicher, T. S. Sandhu, F. W. Hetzel

Henry Ford Hospital Medical Journal

A clinical trial is currently in progress to determine the efficacy of combined fractions of hyperthermia and radiation. The protocol consists of two parts. First, four fractions of microwave-induced hyperthermia (45.0° ± 0.5°C) are applied for 1 1/2 hours to the volume encompassing the tumor, each separated by 72 hours. After a one-week rest, a second series of four fractions is administered again at 72- hour intervals. Each fraction consists of a 400 rad dose of radiation followed within 20 minutes by hyperthermia (42.5 ± 0.5°C) for 1 1/2 hours. Currently, we have treated 62 patients with 82 fields with …

Local Microwave Hyperthermia In Cancer Therapy: Preliminary Report, Carlos A. Perez, Walter Kopecky, Robert Baglan, D. Venkata Rao, Richard Johnson

Henry Ford Hospital Medical Journal

This report summarizes our preliminary experience at the Mallinckrodt Institute of Radiology treating 26 patients with metastatic tumors with hyperthermia and irradiation. Patients received 400 rad fractions given every 72 hours (twice weekly) for a total of 2400, 2800, 3200, or 4000 rad followed by hyperthermia. In addition, 18 lesions were treated according to a protocol designed by the Radiation Therapy Oncology Group (RTOG) for superficial measurable tumors to receive three doses of 500 rad followed by hyperthermia (six lesions) or irradiation alone (three doses of 600, 700, or 800 rad) every 72 hours. Hyperthermia was administered with 2450 MHz …

Clinical Hyperthermia And Irradiation: Pilot Studies, John Fazekas, Ted Nerlinger

Henry Ford Hospital Medical Journal

Phase / (February 1979-May 1980) of our hyperthermia pilot study involved 26 carcinoma patients who participated in two treatment protocols: 20 patients received electron beam x-ray therapy plus an average of eight hyperthermia sessions (twice weekly at 42.5°-43.5°C intralesional temperatures for 40 minutes); 17 patients were heated with a 3450 microwave MHz generator in conjunction with surface heating (circulating water bag at 45°C). AGA thermograms assessed superficial blood flow to document heating patterns and forewarned of potential overheating as manifested by superficial burns (blisters). Although our study Is nonrandomized and our patient numbers are small, we noted a complete response …

Glucose Metabolism In Mouse Tumor And Liver With And Without Hyperthermia, Christian Streffer, Siegelinde Hengstebeck, Peter Tamulevicius

Henry Ford Hospital Medical Journal

We measured levels of glycolytic metabolites in mouse tumor and liver after administering a glucose load of 6 mg/g of body weight and after hyperthermia for one hour at 43°C. Metabolites included glucose, glucose-6-phosphate, fructose-1.6-diphosphate, dihydroxyacetone-phosphate, glycerol-3-phosphate, pyruvate, and lactate, as well as acetoacetate and β-hydroxybutyrate. The combined treatment led to an increase of the lactate level and apparently enhanced glucose degradation. The redoxequilibria states were shifted to the reduced metabolites. It is possible that hypoxia was induced or enhanced, which could have significance for tumor therapy. At later periods after hyperthermia, metabolic alterations occurred that have also been observed …

Local Hyperthermia And Radiation: A Biologically-Oriented Clinical Scheduling, G. Arcangeli, A. Cividalli

Henry Ford Hospital Medical Journal

In a series of animal tumor studies, heat was directly delivered into the tumor mass with an interstitial applicator. After hyperthermia (44°-45°C for 45 minutes) or radiation alone (3x10 Gy), only a slight delay in tumor growth was observed, while the combined treatment, either simultaneous or sequential (i.e., heat delivered four hours after irradiation), appeared to be the most effective. The cure rate in the two combined treatment groups was 100%, in contrast to the lack of cure in the first two groups. The simultaneous use of heat and x-ray appeared to be slightly more effective than the sequential schedule; …

Hyperthermia Treatment Of Experimental Tumors, J. Denekamp, S. A. Hill, F. A. Stewart

Henry Ford Hospital Medical Journal

The therapeutic advantage of combining hyperthermia with x-irradiation to treat tumors depends on whether or not it is possible to achieve greater thermal sensitization of tumors than of normal tissues. To determine such therapeutic gain factors (TGE), we assessed the response of mouse skin and seven transplantable mouse tumors to graded x-ray doses given alone or combined with moderate heat (42.5°C for 60 minutes). We constructed dose response curves for the average early skin reaction and for the induced delay in tumor regrowth to an arbitrarily chosen size. We studied the following areas: 1) the therapeutic gain of combining heat …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Hyperthermia, F. K. Storm

Henry Ford Hospital Medical Journal

This report describes the history of hyperthermia and reviews current forms of treatment at both low (42°-43°C) and higher (<45°C) temperatures. Hyperthermia treatment at low temperatures includes fluid immersion and irrigation, regional perfusion with heated fluids, and electromagnetic radiofrequency waves. Low-temperature hyperthermia has also been combined with radiation therapy and with chemotherapy in recent clinical trials. At higher temperatures, we and other investigators have also had promising, preliminary results in treating tumors safely. With the specialized radiofrequency instrumentation we have developed to apply hyperthermia at any depth without preferential surface tissue heating, further clinical investigation of both superficial and deep internal solid tumors is now possible. Recent data suggest that hyperthermia may be an especially effective form of therapy for larger tumors that resist standard forms of treatment. Clinical trials are now underway to determine the most therapeutic dose/time regimen, to determine toxicity and therapeutic enhancement ratios of combined chemotherapy and x-irradiation with hyperthermia, and to evaluate any changes in the host immune system with such therapies.

Microwave Hyperthermia At 2450 And 915 Megahertz Frequencies, Kenneth H. Luk

Henry Ford Hospital Medical Journal

Microwave hyperthermia at frequencies of 2450 and 915 megahertz (MHz) was used to treat superficial measurable tumors of different histopathologies at various sites. Forty-seven patients received 59 courses of treatment; they were able to tolerate temperatures up to 42.5X1. Although hyperthermia alone (40°-42.5°C) did not produce an effective response, hyperthermia and radiation together (up to 2700 rad in nine fractions over three weeks) produced complete and partial responses in three-fourths of the treatment courses. The combination of hyperthermia and chemotherapy may have great potential, but confirmation of these clinical impressions will depend on a much larger series of patient data. …