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Immunohistochemistry Of The Zebrafish Retina, Alison Ruf
Immunohistochemistry Of The Zebrafish Retina, Alison Ruf
Williams Honors College, Honors Research Projects
The retina is made up of many different essential parts that work together to allow for vision of many different species. Some of these crucial cells and proteins include ganglion cells, bipolar cells, photoreceptors, synaptic markers, such as VGLUT, and receptors that bind glutamate. The basis of this project is to use immunohistochemistry to identify these important aspects of the retina in zebrafish via monoclonal and polyclonal antibodies. The goal being to compare specific cells in a control group retina to an experimental group of zebrafish.
Effect Of Vaping And Estrogen/Progesterone On Coagulation Of Blood In Danio Rerio: A Literature Review, Kristen Mccausland
Effect Of Vaping And Estrogen/Progesterone On Coagulation Of Blood In Danio Rerio: A Literature Review, Kristen Mccausland
Williams Honors College, Honors Research Projects
With an increase in popularity among young adults using vaping devices (electronic cigarettes) instead of cigarettes, the effects of these vapors combined with other substances, such as oral contraceptives, is not widely studied. Women who smoke traditional cigarettes while taking oral contraceptives have a greater risk for cardiovascular disease, stroke, and thromboembolism. Due to similarities between cigarettes and e-cigarettes, further studies need to examine the effects of e-cigarettes while taking oral contraceptives. This literature review discusses the hypotheses associated with the combined use of vaping devices while taking oral contraceptives. Proposed experimentation includes zebrafish as the model organism. The fish …
Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara
Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara
Williams Honors College, Honors Research Projects
I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …