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Full-Text Articles in Life Sciences
The Biobehavioral Effects Of Embryonic Exposure To Neural Inflammation And Oxidative Stress In Zebrafish, Dalton J. Anderson
The Biobehavioral Effects Of Embryonic Exposure To Neural Inflammation And Oxidative Stress In Zebrafish, Dalton J. Anderson
Honors College Theses
The purpose of this research is to improve understanding of the neurodevelopmental effects of embryonic exposure to elevated inflammation and oxidative stress induced by the antipyretic drug acetaminophen (APAP). Our study was the first to examine the interactive effects of APAP and inflammation in zebrafish embryos and how the treatments affect brain development and larval behavior. Experimental groups of zebrafish larvae were exposed to lipopolysaccharide (LPS) to induce inflammation, APAP, or LPS + APAP and larval behavior was analyzed using Ethovision automated behavioral tracking software. We also measured changes in whole-brain Glycogen Synthase Kinase 3 Beta (GSK3B) and GSK3B phosphorylation, …
Immunohistochemistry Of The Zebrafish Retina, Alison Ruf
Immunohistochemistry Of The Zebrafish Retina, Alison Ruf
Williams Honors College, Honors Research Projects
The retina is made up of many different essential parts that work together to allow for vision of many different species. Some of these crucial cells and proteins include ganglion cells, bipolar cells, photoreceptors, synaptic markers, such as VGLUT, and receptors that bind glutamate. The basis of this project is to use immunohistochemistry to identify these important aspects of the retina in zebrafish via monoclonal and polyclonal antibodies. The goal being to compare specific cells in a control group retina to an experimental group of zebrafish.
Understanding The Effects Of Embryonic Hyperglycemia On Retinal Development And Maintenance, Kayla Titialii-Torres
Understanding The Effects Of Embryonic Hyperglycemia On Retinal Development And Maintenance, Kayla Titialii-Torres
Theses and Dissertations--Biology
Hundreds of millions of people are affected by diabetes worldwide. Whether they are diagnosed with prediabetes or Type I or II diabetes, there are a variety of mechanisms in the pathogenesis of diabetes. Diabetes is a disease which consists of recurring states of hyperglycemia that can be difficult to manage due to either lack of insulin production or improper utilization of insulin. While these mechanisms of action differ, complications induced by diabetes occur in both poorly regulated Type I and II. Common complications of diabetes include nerve damage, kidney damage, and eye damage. Eye damage specifically is called diabetic retinopathy …
The Neuroinflammatory Effects Of Chronic Unpredictable Stress On Zebrafish, Danio Rerio, Brandon Stewart
The Neuroinflammatory Effects Of Chronic Unpredictable Stress On Zebrafish, Danio Rerio, Brandon Stewart
Master of Science in Integrative Biology Theses
Stress is a state of threatened homeostasis counteracted by various physiologic and behavioral responses aimed to maintain or restore balance. As such, stress acts as a motivator to perform during the challenges of life to survive. Chronic perturbations to the stress response homeostasis without relief can lead to dysregulation, thus attenuating organ systems and structures and causing significant damage {1]. Individuals who undergo psychological trauma endure an acute and transient experience, which results in minimal functional impairment, but some suffer from a chronic condition called posttraumatic stress disorder (PTSD). Individuals who have PTSD are likely to experience intense stress, …
Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara
Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara
Williams Honors College, Honors Research Projects
I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …
Effect Of Vaping And Estrogen/Progesterone On Coagulation Of Blood In Danio Rerio: A Literature Review, Kristen Mccausland
Effect Of Vaping And Estrogen/Progesterone On Coagulation Of Blood In Danio Rerio: A Literature Review, Kristen Mccausland
Williams Honors College, Honors Research Projects
With an increase in popularity among young adults using vaping devices (electronic cigarettes) instead of cigarettes, the effects of these vapors combined with other substances, such as oral contraceptives, is not widely studied. Women who smoke traditional cigarettes while taking oral contraceptives have a greater risk for cardiovascular disease, stroke, and thromboembolism. Due to similarities between cigarettes and e-cigarettes, further studies need to examine the effects of e-cigarettes while taking oral contraceptives. This literature review discusses the hypotheses associated with the combined use of vaping devices while taking oral contraceptives. Proposed experimentation includes zebrafish as the model organism. The fish …
The Developmental And Behavioral Effects Of Δ9-Tetrahydrocannabinol On A Spastic Mutant, Victoria Mendiola
The Developmental And Behavioral Effects Of Δ9-Tetrahydrocannabinol On A Spastic Mutant, Victoria Mendiola
Master of Science in Integrative Biology Theses
Hyperekplexia is a neurological disorder affecting infants and can be characterized by excessive startle reactions in response to unexpected stimuli. This disease can be modeled in zebrafish in the bandoneon (beo) mutant, where the glrbbmutation results in glycine receptor (GLYR) loss of function glycine receptors and ultimately, improper inhibitory signaling. Though hyperekplexia is a glycinergic-based signaling disorder, disease symptoms are often alleviated with GABA-agonistic benzodiazepines. Exogenous cannabinoids also modulate GABAergic signaling, and THC, one of the natural compounds that prompted the discovery of the endocannabinoid system mimics the effects of endogenous cannabinoids at endocannabinoid and non-cannabinoid sites in the …
Nociception And The Experience Of Pain Signaling Due To Nerve Damage, Crystal J. Smith
Nociception And The Experience Of Pain Signaling Due To Nerve Damage, Crystal J. Smith
Master of Science in Integrative Biology Theses
Hyperalgesia is the increased sensitivity to pain which can present itself to many people no matter age or ethnicity. Having the ability to alleviate pain whether it be acute or chronic pain without the use of addictive medication can have the potential to change the course or modern medicine known today. Through the use of zebrafish, this thesis provides a preliminary understanding in pain signal blocking. Three distinct genes have found to be associated with perceiving pain, which include Transient Receptor Potential Ankyrin 1 (TRPA1), Transient Receptor Potential Melestatin 8 (TRPM8), and Cannabinoid Receptor 1 (CNR1). We hypothesize through knockdown …
The Effects Of Amphetamine Exposure On Neurodevelopment And Behavior In Early Life Stages Of Danio Rerio, Jennifer D. Bullard
The Effects Of Amphetamine Exposure On Neurodevelopment And Behavior In Early Life Stages Of Danio Rerio, Jennifer D. Bullard
Master of Science in Integrative Biology Theses
Amphetamines are frequently prescribed to young children for attention deficit hyperactivity disorder (ADHD). Due to the recent rise in ADHD diagnoses and the resulting increase in amphetamine intake in children, we investigated how amphetamine exposure impacts the development of stereotyped behaviors and the neural circuit that governs these behaviors. The zebrafish (Danio rerio) was used as our model organism because of the extensively studied developmental milestones these fish provide. Using escape responses as a behavior model, we exposed embryos to three doses of amphetamine (10 μg/ml, 20 μg/ml, and 30 μg/ml) environmentally during the first 48 hours of …