Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- Wright State University (26)
- The Texas Medical Center Library (12)
- East Tennessee State University (9)
- University of Arkansas, Fayetteville (4)
- University of Massachusetts Amherst (4)
-
- Virginia Commonwealth University (3)
- Western University (3)
- Loyola University Chicago (2)
- University of Kentucky (2)
- University of Louisville (2)
- University of New Mexico (2)
- University of South Carolina (2)
- Wayne State University (2)
- Wilfrid Laurier University (2)
- City University of New York (CUNY) (1)
- Duquesne University (1)
- Georgia Southern University (1)
- Grand Valley State University (1)
- Illinois State University (1)
- Louisiana State University (1)
- Missouri State University (1)
- Nova Southeastern University (1)
- Old Dominion University (1)
- Seton Hall University (1)
- St. Mary's University (1)
- Syracuse University (1)
- The University of Maine (1)
- The University of Southern Mississippi (1)
- University at Albany, State University of New York (1)
- University of Nebraska Medical Center (1)
- Publication Year
- Publication
-
- Browse all Theses and Dissertations (26)
- Electronic Theses and Dissertations (13)
- Dissertations & Theses (Open Access) (12)
- Doctoral Dissertations (4)
- Theses and Dissertations (4)
-
- Electronic Thesis and Dissertation Repository (3)
- Biological Sciences Undergraduate Honors Theses (2)
- Biomedical Sciences ETDs (2)
- Dissertations (2)
- Graduate Theses and Dissertations (2)
- Senior Theses (2)
- Theses and Dissertations (Comprehensive) (2)
- Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics (2)
- All HCAS Student Capstones, Theses, and Dissertations (1)
- Arts & Sciences Electronic Theses and Dissertations (1)
- Biotechnology Theses (1)
- Culminating Experience Projects (1)
- Dissertations, Theses, and Capstone Projects (1)
- Graduate Theses, Dissertations, and Problem Reports (1)
- Honors Capstone Projects - All (1)
- Honors College Theses (1)
- Honors Program Theses and Research Projects (1)
- LSU Doctoral Dissertations (1)
- Legacy Theses & Dissertations (2009 - 2024) (1)
- MSU Graduate Theses (1)
- Master's Theses (1)
- Masters Theses (1)
- Seton Hall University Dissertations and Theses (ETDs) (1)
- Theses & Dissertations (1)
- Theses and Dissertations in Biomedical Sciences (1)
Articles 91 - 97 of 97
Full-Text Articles in Life Sciences
Cd40-Mediated Signaling Of Interleukin-1(Beta) Synthesis And Rescue From Apoptosis In Monocytes: Modulation By Il-4 And Il-10, Jonathan C. Poe
Cd40-Mediated Signaling Of Interleukin-1(Beta) Synthesis And Rescue From Apoptosis In Monocytes: Modulation By Il-4 And Il-10, Jonathan C. Poe
Electronic Theses and Dissertations
To date, the cellular mechanisms involved in the progression of diseases characterized by chronic inflammation, such as rheumatoid arthritis (RA), remain largely unknown. However, cell-to-cell contact interactions between CD4+ helper T (Th) cells and monocytes have been implicated in the induction and maintenance of pro-inflammatory cytokine synthesis that is characteristic to the pathogenesis of RA. One such cytokine produced during monocyte-Th cell contact is interleukin (IL)-1 β, a mediator directly involved in the characteristic tissue destruction that occurs in the synovia of individuals with RA. Previous studies in our laboratories have shown that ligation of CD40 on monocytes with CD40 …
Role Of The Cd40-Cd40 Ligand Interaction In Cd4(+) T Cell Activation Of Monocyte Interleukin-1 Synthesis, David H. Wagner
Role Of The Cd40-Cd40 Ligand Interaction In Cd4(+) T Cell Activation Of Monocyte Interleukin-1 Synthesis, David H. Wagner
Electronic Theses and Dissertations
Most studies of the induction of cytokine synthesis in monocytes have used an exogenous triggering agent such as Lipolpoysaccharide (LPS). However, during nonseptic chronic inflammatory responses (e.g., rheumatoid arthritis) monocyte activation occurs as a result of T cell generated signals. This report demonstrated that plasma membranes from anti-CD3 activated peripheral CD4$\sp{+}$ T cells (Tm$\sp{\rm A}$) but not from resting CD4$\sp{+}$ cells (Tm$\sp{\rm R}$) induced monocytes to synthesize IL-1 in the absence of costimulatory cytokines. The expression kinetics of the molecule(s) unique to activated T cells which interact with monocyte receptors to induce IL-1 demonstrated that optimal expression occurred at 6h …
Nitric Oxide Production: A Mechanism For Inhibition Of Chlamydia Trachomatis Replication, Bojun Chen
Nitric Oxide Production: A Mechanism For Inhibition Of Chlamydia Trachomatis Replication, Bojun Chen
Electronic Theses and Dissertations
Chlamydia trachomatis (CT) replicates in macrophages, but is inhibited by IFN-$\gamma$ or LPS. IFN-$\gamma$ and/or LPS induced nitrite production in mouse peritoneal macrophages, macrophage cell lines (RAW264.7 and J774A.1) and McCoy cells. Kinetic studies indicated that peak production occurred 48 hours post-treatment. CT infection itself was insufficient to induce nitrite production, but resulted in enhancement of nitrite production in IFN-$\gamma$-treated cells. Treatment with IFN-$\gamma$ or LPS resulted in significant inhibition of CT replication in these cells. Strong correlation between nitrite production and inhibition of CT replication was observed in RAW264.7 and J774A.1 cells (correlation coefficients: $-$0.93 and $-$0.94, p $<$ 0.001). N$\sp{\rm g}$- monomethyl-L-arginine (L-NMMA) specifically inhibited nitrite production and partially reversed inhibition of CT replication in macrophage cell lines. NOS mRNA was measured in RAW264.7 cells by Northern blot and Dot blot hybridization. Strong correlation between NOS mRNA expression and inhibition of CT replication (correlation coefficient: $-$0.97, p $<$ 0.05) was observed. Anti-TNF-$\alpha$ antibody completely neutralized the biological activity of TNF-$\alpha$ secreted by LPS-treated RAW264.7 cells, yet the antibody neither reduced nitrite production nor restored CT replication. Combination of the antibody and L-NMMA significantly enhanced restoration of CT replication. In peritoneal macrophages, inhibition of CT replication induced by IFN-$\gamma$ was partially restored by L-NMMA or anti-TNF-$\alpha$ antibody. In McCoy cells, inhibition of CT replication induced by IFN-$\gamma$ and LPS was not significantly restored by L-NMMA. Great restoration of CT replication by 1 mM L-NMMA was observed in LPS-treated J774A.1 cells (31%), but not in IFN-$\gamma$-treated cells (5%). Our data indicate that (1) NO production is one of the mechanisms for inhibition of CT replication in IFN-$\gamma$-activated peritoneal macrophages and RAW264.7 cells; (2) NO plays a significant role in CT inhibition in LPS-treated macrophage cell lines, but not peritoneal macrophages; (3) TNF-$\alpha$ may be associated with inhibition, but the mechanism(s) may not involve NO production; (4) NO production may not be the mechanism for CT inhibition in McCoy cells treated with IFN-$\gamma$ and LPS.
Mechanisms Of T Cell-Mediated Macrophage Activation: Role Of Antigen Specific And Antigen Nonspecific Cognate Interactions, Xiang Tao
Electronic Theses and Dissertations
Macrophages play an important role in host antimicrobial immunity and in non-septic inflammatory reactions. Most studies on macrophage activation have focused on the roles of the T cell-produced cytokine, interferon-$\gamma$ (IFN$\gamma)$ and bacterial product, lipopolysaccharide (LPS). T cell-macrophage interaction is a critical step in initiating both specific and nonspecific immune responses to antigenic stimulation. The current study examines the role of cognate T cell-macrophage interaction in activation of macrophage effector functions and induction of macrophage early activation gene expression. Viable resting T$\sb{\rm H}$2 clone cells can activate IFN$\gamma$-primed macrophages to produce reactive nitrogen intermediates (RNI) or express cytostatic activity. The …
Probing Protein-Protein Interactions Among Proteins Of A Nonaggregated Fatty Acid Synthetase From Euglena Gracilis Variety Bacillaris, Sande G. Williams
Probing Protein-Protein Interactions Among Proteins Of A Nonaggregated Fatty Acid Synthetase From Euglena Gracilis Variety Bacillaris, Sande G. Williams
Electronic Theses and Dissertations
Enoyl-acyl carrier protein (ACP) reductase from chloroplast nonaggregated fatty acid synthetase (FAS) of Euglena gracilis variety bacillaris was purified to a single band on a denaturing polyacrylamide gel. The enzyme was partially characterized with respect to substrate specificity, reduced nucleotide requirement, and the effect of ACP and Ca$\sp{++}$ on enzyme activity. Antibodies against the purified protein were raised in hens and isolated from eggs. ACP was purified from Euglena in yields of about 1mg/100g (wet weight) of cells. Antibodies were raised against the purified protein. ACP antibodies inhibited the Euglena chloroplast FAS using Euglena or E. coli ACP as a …
Generation, Isolation And Assay Methods For Human Lymphocyte Mitogenic Factor, Thomas E. Seay
Generation, Isolation And Assay Methods For Human Lymphocyte Mitogenic Factor, Thomas E. Seay
Electronic Theses and Dissertations
Activated lymphocytes secrete many products including the lymphokine human lymphocyte mitogenic factor (HLMF). In preliminary experiments lymphocytes from peripheral blood and palatine tonsils were evaluated as possible sources of HLMF by evaluating their level of activation through screening their spontaneous and concanavalin A (con A)-induced blastogenic responses. Tonsil lymphocytes (TL) were found to have high spontaneous proliferation as compared to peripheral blood lymphocytes (PBL). Cells from both sources responded to con A by undergoing a typical blastogenic response. Because TL must be obtained septically, they are frequently cultured in the presence of the antimycotic agent, Amphotericin B (Am B). Since …
Tonsil Cell Products Which Modify In Vitro Proliferation Of Blood Lymphocytes, Thomas W. Hodge
Tonsil Cell Products Which Modify In Vitro Proliferation Of Blood Lymphocytes, Thomas W. Hodge
Electronic Theses and Dissertations
Human palatine tonsil lymphocytes, when compared to peripheral blood lymphocytes (PBL), were in an activated state even though there was no in vitro stimulation. When these tonsil lymphocytes were cultured in the absence of serum and polyclonal mitogens or antigens, the supernatant fluid often inhibited the proliferative response of target PBL to con A. The extent of this suppression ranged from 22% to 84%, and target cell viability was 90% or greater. There was no evidence for the presence of immunoglobulins or (alpha)2-macroglobulin in whole supernatant fluids. The suppressor was partially denatured at 80(DEGREES)C and was rendered completely inactive upon …