Life Sciences Commons^™

Evaluation Of Systemic Imidacloprid And Herbicide Treatments On Flatheaded Borer (Coleoptera: Buprestidae) Management In Field Nursery Production, Karla M. Addesso, Jason B. Oliver, Nadeer N. Youssef, Donna C. Fare

Agricultural and Environmental Sciences Faculty Research

The flatheaded appletree borer, Chrysobothris femorata (Olivier) (Coleoptera: Buprestidae), and related species are deciduous tree pests. Female beetles prefer to oviposit at tree bases, and larvae tunnel beneath the bark, which weakens or kills young or newly transplanted trees. In the first objective of this study, Discus N/G (2.94% imidacloprid + 0.7% cyfluthrin) applied at six lower-than-labeled rates (0.0, 0.98, 1.97, 3.94, 5.91, and 7.87 ml/cm of average trunk dia.) was evaluated for protection of field-grown maples. A second objective evaluated imidacloprid with and without herbicides to assess the impact of weed competition at the tree base on insecticide effectiveness. …

The Impact Of Plant Essential Oils And Fine Mesh Row Covers On Flea Beetle (Chrysomelidae) Management In Brassicaceous Greens Production, Robert Brockman, Ryan Kuesel, Kendall Archer, Kyla O’Hearn, Neil Wilson, Delia W. Scott, Mark A. Williams, Ricardo Bessin, David J. Gonthier

Entomology Faculty Publications

Brassicaceous leafy greens are an important crop for small growers but are difficult to produce due to damage by flea beetles. Flea beetles are problematic for growers as they chew many small holes through leaves rendering produce unmarketable. We tested the efficacy of several essential oils, the woven-mesh row cover ProtekNet, and the spunbonded row cover Agribon, compared to organic and conventional insecticides and no spray controls in the spring and fall of 2019. We found that the two row cover treatments (Agribon and ProtekNet) provided the best control of flea beetles and associated damage. Thyme oil was highly phytotoxic …

Hu Aquaponics Monitoring And Control System : European Annual Edunet Conference 2020, Rachel L. Fogle, Glenn P. Williams, Josh R. Krug

Presidential Research Grants

The functional purpose of the HU Aquaponics Monitoring and Control System Project is to develop an environmental and plant monitoring and control system for the HU Aquaponics Lab, located in the Student Union. The project involves the design and implementation of technology that will regularly take measurements from the environment (e.g., air temperature, water temperature, pH, dissolved oxygen, etc). PLCnext Technology will systematically collect, store, and web-publish the measurement data for HU researchers and the public to use for scientific research.

Introgression Of The Afila Gene Into Climbing Garden Pea (Pisum Sativum L.), Oscar Eduardo Checa, Marino Rodriguez, Xingbo Wu, Matthew Wohlgemuth Blair

Agricultural and Environmental Sciences Faculty Research

The pea (Pisum sativum L.) is one of the most important crops in temperate agriculture around the world. In the tropics, highland production is also common with multiple harvests of nearly mature seeds from climbing plant types on trellises. While the leafless variant caused by the afila gene is widely used in developing row-cropped field peas in Europe, its use for trellised garden peas has not been reported. In this study we describe a pea breeding program for a high-elevation tropical environment in the Department of Nariño in Colombia, where over 16,000 hectares of the crop are produced. The most …

Nebline, Sept./Oct. 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Fall Invaders: What You Can Do

Extension Partners With LPS on Handwashing Videos

Plant A Row for the Hungry

Recipe of the Month

Pruning Everbearing Raspberries

Seasonal Needle Drop on Evergreens

Garden Guide: Things to Do This Month

Disasters Don't Wait. Make Your Plan Today!

Extension Offers Training for Emergency Preparedness and Coping

Upcoming Early Childhood Trainings

Nitrate in Drinking Water

Heart of 4-H Volunteer Award: Tracy Pracheil

Heart of 4-H Volunteer Award: Amy Vander Woude

4-H Announcements for enrolled 4-H'ers or 4-H volunteers

4-H Information Night

State 4-H Horse Expo Top Results

4-H at Super Fair a Blend of …

Economics Of Herbicide Programs For Weed Control In Conventional, Glufosinate, And Dicamba/Glyphosate-Resistant Soybean Across Nebraska, Adam M. Striegel, Kent M. Eskridge, Nevin C. Lawrence, Stevan Knezevic, Greg R. Kruger, Christopher A. Proctor, Gary Hein, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Despite widespread adoption of dicamba/glyphosate-resistant (DGR) soybean [Glycine max (L.) Merr.] in Nebraska and across the United States in recent years, economic information comparing herbicide programs with glufosinate-resistant (GLU-R) and conventional soybean is not available. The objectives of this study were to evaluate weed control efficacy, crop safety, gross profit margin, and benefit/cost ratios of herbicide programs with multiple sites of action in DGR, GLUR, and conventional soybean. Field experiments were conducted in 2018 and 2019 at three irrigated and two rain-fed locations across Nebraska, for a total of 10 site-years. Herbicides applied pre-emergence (PRE) that included herbicides with …

Coal Char Affects Soil Ph To Reduce Ammonia Volatilization From Sandy Loam Soil, Dinesh Panday, Maysoon M. Mikha, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Ammonia (NH₃) volatilization loss adversely affects N availability in soil-plant systems, reduces crop yield, and negatively impacts environment. Char (coal combustion residue), which contains up to 293 g kg⁻¹ total C by weight, has been shown to reduce NH₃ volatilization due to its considerably high surface area and cation exchange capacity. The NH₃ loss can be greatly affected by a shift in soil pH or urea hydrolysis. A 21-d laboratory study was conducted to evaluate the effects of char on soil pH, N transformations, and subsequent NH₃ volatilization in sandy loam soil. Two …

Rhizobacterial Species Richness Improves Sorghum Growth And Soil Nutrient Synergism In A Nutrient-Poor Greenhouse Soil, Mohammad Radhi Sahib, Zahida H. Pervaiz, Mark A. Williams, Muhammad Saleem, Seth Debolt

Horticulture Faculty Publications

Although microbes influence plant growth, little is known about the impact of microbial diversity on plant fitness trade-offs, intraspecific-interactions, and soil nutrient dynamics in the context of biodiversity-ecosystem functioning (BEF) research. The BEF theory states that higher species richness can enhance ecosystem functioning. Thus, we hypothesize that rhizobacterial species richness will alter sorghum (Sorghum bicolor L.) growth, soil nutrient dynamics and interactions (antagonism or synergism) in a nutrient-poor greenhouse soil. Using six rhizobacterial species in a BEF experiment, we tested the impact of a species richness gradient (0, 1, 3, 5 or 6 species per community) on plant growth, …

Registration Of ‘Ne10589’ (Husker Genetics Brand Ruth) Hard Red Winter Wheat, P. Stephen Baenziger, Robert Graybosch, Devin J. Rose, Lan Xu, Mary Guttieri, Teshome Regassa, Robert N. Klein, Greg R. Kruger, Dipak Santra, Gary Hergert, Stephen Wegulo, Y. Jin, J. Kolmer, Gary Hein, Jeff Bradshaw, M.-S. Chen, G. Bai, R. L. Bowden, Ibrahim El-Basyoni, A. Lorenz

Department of Agronomy and Horticulture: Faculty Publications

With climate variation common in the U.S. Great Plains and particularly in Nebraska, wheat growers prefer broadly adapted cultivars. ‘NE10589’ (Reg. no. CV-1165, PI 675998) hard red winter wheat (Triticum aestivum L.) was developed cooperatively by the Nebraska Agricultural Experiment Station and the USDA– ARS and released in January 2015 by the developing institutions. NE10589 was released primarily as a broadly adapted semi-dwarf cultivar for its superior performance under rainfed conditions throughout Nebraska and adjacent areas of the Great Plains. Its broad adaptation ensures that it will perform well under the typical environmental fluctuations that occur inNebraska. NE10589was selected …

Greenhouse Spatial Effects Detected In The Barley (Hordeum Vulgare L.) Epigenome Underlie Stochasticity Of Dna Methylation, Moumouni Konate, Michael J. Wilkinson, Julian Taylor, Eileen S. Scott, Bettina Berger, Carlos M. Rodriguez Lopez

Horticulture Faculty Publications

Environmental cues are known to alter the methylation profile of genomic DNA, and thereby change the expression of some genes. A proportion of such modifications may become adaptive by adjusting expression of stress response genes but others have been shown to be highly stochastic, even under controlled conditions. The influence of environmental flux on plants adds an additional layer of complexity that has potential to confound attempts to interpret interactions between environment, methylome, and plant form. We therefore adopt a positional and longitudinal approach to study progressive changes to barley DNA methylation patterns in response to salt exposure during development …

Rapid In Vitro Multiplication Of Non-Runnering Fragaria Vesca Genotypes From Seedling Shoot Axillary Bud Explants, Babul C. Sarker, Douglas D. Archbold, Robert L. Geneve, Sharon T. Kester

Horticulture Faculty Publications

Fragaria vesca L. has become a model species for genomic studies relevant to important crop plant species in the Rosaceae family, but generating large numbers of plants from non-runner-producing genotypes is slow. To develop a protocol for the rapid generation of plants, leaf explants were compared to single axillary bud shoot explants, both from in vitro-grown Fragaria vesca seedlings, as sources of shoots for new plant production in response to benzyladenine (BA) or thidiazuron (TDZ) combined with indolebutyric acid (IBA) on Murashige and Skoog’s Basal Salt (MS) medium. BA at 2.0 and 4.0 mg L⁻¹ and TDZ at 1.5 …

Simulating Winter Rye Cover Crop Production Under Alternative Management In A Corn-Soybean Rotation, Nilovna Chatterjee, Sotirios V. Archontoulis, Angela M Bastidas, Christopher A. Proctor, Roger W. Elmore, Andrea Basche

Department of Agronomy and Horticulture: Faculty Publications

The Agricultural Production Systems sIMulator (APSIM) was used to evaluate two alternative approaches for extending the cover crop growing window into corn (Zea mays L.) and soybean (Glycine max L.) crop rotations in Nebraska, USA.We evaluated how: (i) shifting corn planting dates (mid-April to early-June) and (ii) altering comparative relative maturity (CRM) corn hybrids (80 to 115 days) influence cover crop biomass and corn yields over a 30-year period. The APSIM model was tested using experimental data and was then used to simulate a range of cover crop planting and termination scenarios. Our results showed no significant yield …

Selection Signatures Across Seven Decades Of Hard Winter Wheat Breeding In The Great Plains Of The United States, Habtamu Ayalew, Mark E. Sorrells, Brett F. Carver, P. Stephen Baenziger, Xue-Feng Ma

Department of Agronomy and Horticulture: Faculty Publications

Classical plant breeding has been instrumental in changing the genetic makeup of crop plants for better ecological adaptation and improved quality. This paper provides insights of the genomic changes effected in hard winter wheat (Triticum aestivum L.) through decades of breeding and selection in the Great Plains of the United States. Population structure and differentiation analyses were conducted on 185 wheat cultivars released from 1943 to 2013. Cultivars were grouped into four distinct clusters using discriminant analysis of principal components (DAPC). One of the clusters was unique in that 15 out of the 18 individuals were recent releases (2000–2010), …

Arkansas Wheat Cultivar Performance Tests 2019-2020, R. E. Mason, R. G. Miller, D. E. Moon, J. P. Kelley, J. Carlin

Arkansas Agricultural Experiment Station Research Series

Wheat cultivar performance tests are conducted each year in Ark- ansas by the University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station, Department of Crop, Soil, and Environmental Sciences. The tests provide informa- tion to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for small-grain producers. The tests are conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Newport Extension Center near Newport, the Rohwer Research Station near Rohwer, the Pine …

Permethrin Residual Activity Against Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) Attacks Following Field Aging And Simulated Rainfall Weathering, Matthew S. Brown, Karla M. Addesso, Fulya Baysal-Gurel, Nadeer N. Youssef, Jason B. Oliver

Agricultural and Environmental Sciences Faculty Research

Adult ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) bore into ornamental nursery trees resulting in trunk vascular tissue damage, which can potentially kill trees. Ambrosia beetle exposure to surface-applied insecticides is minimal after internal trunk galleries are formed, so effective management requires insecticide treatments to be applied near the time of infestation or to have residual activity on the bark. Tree trunk sections (bolts) were used to determine the effect of field aging or irrigation (i.e., simulated rainfall weathering) on permethrin residual activity against ambrosia beetles. In all experiments, 30-cm-long bolts from Liriodendron tulipifera L. (Magnoliales: Magnoliaceae) were hollowed and filled with …

Estimating Economic Minimums Of Mowing, Fertilizing, And Irrigating Turfgrass, Douglas J. Soldat, James T. Brosnan, Ambika Chandra, Roch E. Gaussoin, Alec Kowalewski, Bernd Leinauer, Frank S. Rossi, John C. Stier, J Bryan Unruh

Department of Agronomy and Horticulture: Faculty Publications

The public health crisis and economic recession caused by the COVID-19 pandemic have forced turfgrass industry professionals to re-evaluate standard practices. Minimum costs required to fertilize, irrigate, and mow turfgrasses can be roughly estimated using climate data, turfgrass physiology information, and resource costs. Although the actual minimum costs vary situationally and regionally, mowing golf putting greens optimally requires about US$34 per acre per month, whereas other turfgrass areas cost less than US$11 per acre per growing month. Fertilizer applications to turfgrass cost US$22 or less per acre per growing month. Irrigation costs (water and electricity for pumping) vary widely, with …

Evapotranspiration Of Urban Landscape Trees And Turfgrass In An Arid Environment: Potential Trade-Offs In The Landscape, Tamara Wynne, Dale Devitt

Life Sciences Faculty Research

Irrigation in arid urban landscapes can use significant amounts of water. Water conservation must be based on plant species and the ability to meet plant water requirements while minimizing overirrigation. However, actual evapotranspiration (ET) estimates for landscape trees and turfgrass in arid environments are poorly documented, especially direct comparisons to assess potential trade-offs. We conducted research to quantify ET of 10 common landscape tree species grown in southern Nevada and compared these values with the ET of both a warm season and cool season turfgrass species. The trees were grown in a plot with a high-density planting (256 trees/ha). A …

Soybean Barcsoysnp6k: An Assay For Soybean Genetics And Breeding Research, Qijian Song, Long Yan, Charles Quigley, Edward Fickus, He Wei, Linfeng Chen, Faming Dong, Susan Araya, Jinlong Liu, David Hyten, Vincent R. Pantalone, Randall L. Nelson

Department of Agronomy and Horticulture: Faculty Publications

The limited number of recombinant events in recombinant inbred lines suggests that for a biparental population with a limited number of recombinant inbred lines, it is unnecessary to genotype the lines with many markers. For genomic prediction and selection, previous studies have demonstrated that only 1000–2000 genome-wide common markers across all lines/accessions are needed to reach maximum efficiency of genomic prediction in populations. Evaluation of too many markers will not only increase the cost but also generate redundant information. We developed a soybean (Glycine max) assay, BARCSoySNP6K, containing 6000 markers, which were carefully chosen from the SoySNP50K assay based …

Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang

Department of Agronomy and Horticulture: Faculty Publications

Alfalfa (Medicago sativa L.) is one of the most important forage crops throughout the world. Maximizing leaf retention during the haymaking process is critical for achieving superior hay quality and maintaining biomass yield. Leaf abscission process affects leaf retention. Previous studies have largely focused on the molecular mechanisms of floral organ, pedicel and seed abscission but scarcely touched on leaf and petiole abscission. This study focuses on leaf and petiole abscission in the model legume Medicago truncatula and its closely related commercial species alfalfa. By analysing the petiolule-like pulvinus (plp) mutant in M. truncatula at phenotypic level (breakstrength and shaking …

Shotgun Metagenomic Sequencing Data Of Sunflower Rhizosphere Microbial Community In South Africa, Olubukola Oluranti Babalola, Temitayo Tosin Alawiye, Carlos M. Rodriguez Lopez, Ayansina Segun Ayangbenro

Horticulture Faculty Publications

This dataset presents shotgun metagenomic sequencing of sunflower rhizosphere microbiome in Bloemhof, South Africa. Data were collected to decipher the structure and function in the sunflower microbial community. Illumina HiSeq platform using next generation sequencing of the DNA was carried out. The metagenome comprised 8,991,566 sequences totaling 1,607,022,279 bp size and 66% GC content. The metagenome was deposited into the NCBI database and can be accessed with the SRA accession number SRR10418054. An online metagenome server (MG RAST) using the subsystem database revealed bacteria had the highest taxonomical representation with 98.47%, eukaryote at 1.23%, and archaea at 0.20%. The most …

Nebline, August 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Be an Active Family with Outdoor Activities

EXTENSION OFFICE OPEN

Recipes of the month

What You Need to Know About Having a Well Drilled

Almost Time for Fall Lawn Seeding

Chiggers: Itching in all the Wrong Places

Collect – Share – Practice: Family Emergency Communication Plans

August Blooming Perennial Flowers

Garden Guide: Things to Do This Month

Creating Reading Routines During the Summer Months

HEART OF 4-H

4-H Youth and Volunteers Sew 1,789 Hats and Masks for Bryan Health

4-H’ers Problem Solve Real-Life Situations at County Life Challenge

Virtual State Public Speaking Contest Results

State Fair to Focus on Showcasing …

B.R. Wells Arkansas Rice Research Studies 2019, K.A. K. Moldenhauer, B. Scott, J. Hardke

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Influence Of Cover Crop Management Practices On Rainfed Corn Production In Semi-Arid Western Nebraska, Alexandre Tonon Rosa

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

With the increased cover crop (CC) popularity, producers of semi-arid regions of western Nebraska are questioning whether they could successfully incorporate CC into their rainfed winter wheat (Triticum aestivum L.)-corn (Zea mays L.)-fallow rotations. The major concern is that CCs may deplete soil water affecting the subsequent crop. Therefore, three studies were established under rainfed conditions of western Nebraska to access the effects of CCs on soil water, soil compaction, nutrient cycling, weed demographics, residue coverage, and subsequent corn yield. The first study evaluated the influence of CC planting and termination times prior to corn establishment. …

Effects Of Tank Contamination And Impact Of Drift-Reducing Agents On Weed Control In Response To Dicamba Applications, Milos Zaric

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Availability of dicamba-tolerant (DT) crops from 2017 provided farmers with additional herbicides for weed control management in row crops. However, the technology alike this one has concerns regarding dicamba off-target movement (OTM) causing undesirable effects on sensitive vegetation. Even though dicamba has high water solubility OTM that has often been overlooked when it comes to unintended crop exposure is dicamba tank contamination. Considering the complexity of spraying equipment soybean response may be expected even when small amounts of residues are left in the spray equipment. Typically, the same field spray equipment is used to perform herbicide application through growing season …

Preparing And Improving Garden Soil, Katie Wagner, Melanie Stock, Larry A. Sagers

All Current Publications

A great garden starts with healthy soil. In Utah, there are unique considerations for creating and maintaining healthy soils. Native Utah soils are relatively low in organic matter. One of Utah’s most troublesome, pH-related deficiencies is iron chlorosis, a deficiency of plant-available iron. Soil salinity easily increases in Utah. This fact sheet addresses these challenges to help gardeners transform garden soils from brutal to beautiful!

Impact Of Winter Cover Crop Usage In Soilborne Disease Suppressiveness In Woody Ornamental Production System, Milan Panth, Fulya Baysal-Gurel, Terri Simmons, Karla M. Addesso, Anthony L. Witcher

Agricultural and Environmental Sciences Faculty Research

Diseases caused by soilborne pathogens are a major limitation to field grown nursery production. The application of cover crops for soilborne disease management has not been widely investigated in a woody ornamental nursery production system. The objective of this study was to explore the impact of winter cover crops usage on soilborne disease management in that system. Soils from established field plots of red maple (Acer rubrum L.) with and without winter cover crops (crimson clover (Trifolium incarnatum L.) or triticale (× Triticosecale W.)) were sampled following the senescence of the cover crops. Separate bioassays were performed using red maple …

A Mechanistic Model And Therapeutic Interventions For Covid-19 Involving A Ras-Mediated Bradykinin Storm, Michael R. Garvin, Christiane Alvarez, J. Izaak Miller, Erica T. Prates, Angelica M. Walker, B. Kirtley Amos, Alan E. Mast, Amy Justice, Bruce Aronow, Daniel Jacobson

Horticulture Graduate Research

Neither the disease mechanism nor treatments for COVID-19 are currently known. Here, we present a novel molecular mechanism for COVID-19 that provides therapeutic intervention points that can be addressed with existing FDA-approved pharmaceuticals. The entry point for the virus is ACE2, which is a component of the counteracting hypotensive axis of RAS. Bradykinin is a potent part of the vasopressor system that induces hypotension and vasodilation and is degraded by ACE and enhanced by the angiotensin1-9 produced by ACE2. Here, we perform a new analysis on gene expression data from cells in bronchoalveolar lavage fluid (BALF) from COVID-19 patients that …

Perioperative And Oncological Outcomes Following Minimally Invasive Versus Open Pancreaticoduodenectomy For Pancreatic Duct Adenocarcinoma, Rui Sun, Jiawen Yu, Yifan Zhang

Department of Agronomy and Horticulture: Faculty Publications

Background The outcomes of minimally invasive pancreaticoduodenectomy have not been adequately compared with those of open pancreaticoduodenectomy in patients with pancreatic ductal adenocarcinoma. We performed a meta‐analysis to compare the perioperative and oncological outcomes of these two pancreaticoduodenectomy procedures specifically in patients with pancreatic ductal adenocarcinoma.

Methods Before this study was initiated, a specific protocol was designed and has been registered in PROSEPRO (ID: CRD42020149438). Using the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines, PubMed, EMBASE, Web of Science, Cochrane Central Register, and ClinicalTrials.gov databases were systematically searched for studies published between January 1994 and October 2019. …

Evaluating Opportunities For Integrated Crop Livestock Systems In Eastern Nebraska, Elizabeth Kay Christenson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Current agricultural systems focused on production of few commodities are facing production, economic, and environmental challenges. To address these challenges, Integrated Crop Livestock Systems (ICLS) have emerged through three primary methods 1) perennial grasslands for grazing and/or hay production, 2) crop residue grazing, and 3) cover crop grazing. To evaluate potential of ICLS mitigating current challenges, a field scale model ICLS was developed in 2015. The ICLS includes 4-ha each of ‘Newell’ smooth bromegrass (Bromus inermis L.), ‘Liberty’ switchgrass (Panicum virgatum L.), and ‘Shawnee’ switchgrass. The ICLS also included 8-ha of continuous corn (Zea mays L.). In 2016, only hay …

Soil-Nitrogen, Potentially Mineralizable-Nitrogen, And Field Condition Information Marginally Improves Corn Nitrogen Management, Jason Clark, Fabián G. Fernández, Kristen S. Veum, James J. Camberato, Paul R. Carter, Richard Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, Carrie A.M. Laboski, Emerson Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Anaerobic potentially mineralizable nitrogen (PMN) combined with preplant nitrate test (PPNT) or pre-sidedress nitrate test (PSNT) may improve corn (Zea mays L.) N management. Forty-nine corn N response studies were conducted across the U.S. Midwest to evaluate the capacity of PPNT and PSNT to predict grain yield, N uptake, and economic optimal N rate (EONR) when adjusted by soil sampling depth, soil texture, temperature, PMN, and initial NH₄–N from PMN analysis. Pre-plant soil samples were obtained for PPNT (0- to 30-, 30- to 60-, 60- to 90-cm depths) and PMN (0- to 30-cm depth) before corn planting …