Life Sciences Commons^™

Long-Term Sandhills Prairie Responses To Precipitation, Temperature, And Cattle Stocking Rate, John A. Guretzky, Cheryl Dunn, Heidi L. Hillhouse

Department of Agronomy and Horticulture: Faculty Publications

Understanding of Sandhills prairie, the most expansive sand dune region stabilized by perennial grasses in the Western Hemisphere, is limited by lack of long-term vegetation data. We used a 26-year dataset to evaluate Sandhills prairie responses to yearto- year variation in precipitation, temperature, and cattle stocking rate. Basal cover, a measurement that is constant seasonally and used to detect long-term changes in bunchgrass vegetation, was measured in 38–40 permanent plots positioned along four transects spanning 769 ha from 1979 to 2007. Across this period, total basal cover averaged 2.4 % and was dominated by warm-season grasses (81.1 %). Schizachyrium scoparium …

Nebline, June 2016

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: The Garden Grocery — Food Safety & Selection at Farmers’ Markets

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

Extension Calendar

and other extension news and events

The Hiv-1 Tat Protein Is Monomethylated At Lysine 71 By The Lysine Methyltransferase Kmt7, Ibraheem Ali, Holly Ramage, Daniela Boehm, Lynnette M. A. Dirk, Naoki Sakane, Kazuki Hanada, Sara Pagans, Katrin Kaehlcke, Katherine Aull, Leor Weinberger, Raymond Trievel, Martina Schnoelzer, Masafumi Kamada, Robert L. Houtz, Melanie Ott

Horticulture Faculty Publications

The HIV-1 transactivator protein Tat is a critical regulator of HIV transcription primarily enabling efficient elongation of viral transcripts. Its interactions with RNA and various host factors are regulated by ordered, transient post-translational modifications. Here, we report a novel Tat modification, monomethylation at lysine 71 (K71). We found that Lys-71 monomethylation (K71me) is catalyzed by KMT7, a methyltransferase that also targets lysine 51 (K51) in Tat. Using mass spectrometry, in vitro enzymology, and modification-specific antibodies, we found that KMT7 monomethylates both Lys-71 and Lys-51 in Tat. K71me is important for full Tat transactivation, as KMT7 knockdown impaired the transcriptional activity …

Arkansas Soybean Research Studies 2014, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Nebline, May 2016

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Emerald Ash Borer is Getting Closer

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Biology, Ecology, And Management Of Nonnative Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae) In Ornamental Plant Nurseries, Christopher M. Ranger, Michael E. Reding, Peter B. Schultz, Jason B. Oliver, Steve D. Frank, Karla M. Addesso, Juang Hong Chong, Blair Sampson, Christopher Werle, Stanton Gill, Charles Krause

Agricultural and Environmental Sciences Faculty Research

Xylosandrus germanus (Blandford) and Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae) are two of the most damaging nonnative ambrosia beetle pests in ornamental plant nurseries. Adult females tunnel into the stems and branches of host plants to create galleries with brood chambers. Hosts are infected with symbiotic Ambrosiella spp. fungi that serve as food for the larvae and adults. Plants can also become infected with secondary opportunistic pathogens, including Fusarium spp. Both X. germanus and X. crassiusculus have broad host ranges, and infestations can result in “toothpicks” of extruded chewed wood and sap flow associated with gallery entrances, canopy dieback, stem …

Nebline, April 2016

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Climate Impacts on Cropping Systems in Eastern Nebraska

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Special Pullout Section: Weed Awareness

An Analysis Of The Allelopathic Relationship Between Basil (Ocimum Basilicum) And Tomatoes (Solanum Lycopersicum) As An Alternative To Fertilizer, Keondra Jenkins

Student Writing

Allelopathy is a trait within certain organisms that allows them to produce and secrete certain biochemical that have various effects on other organism’s growth, survival, and reproduction processes. This study compared the growth results of the allelopathic relationship between basil and tomatoes against fertilized tomatoes and untreated tomatoes. With the information from this study and conclusions can be made about whether or not allelopathic or “companion planting” can serve as a viable alternative for fertilizer.

After nearly 5 months of growth, the allelopathic properties of basil were found to be equal, if not more, beneficial to the growth of the …

Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Cytosine DNA methylation (CDM) is a stable epigenetic modification to the genome and a widespread regulatory process in living organisms that involves multicomponent molecular machines. Genome-wide cytosine methylation patterning participates in the epigenetic reprogramming of a cell, suggesting that the biological information contained within methylation positions may be amenable to decoding. Adaptation to a new cellular or organismal environment also implies the potential for genome-wide redistribution of CDM changes that will ensure the stability of DNA molecules. This raises the question of whether or not we would be able to sort out the regulatory methylation signals from the CDM background …

Ws-2 Introgression In A Proportion Of Arabidopsis Thaliana Col-0 Stock Seed Produces Specific Phenotypes And Highlights The Importance Of Routine Genetic Verification, Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Arabidopsis thaliana is an important model organism with a robust network of resources that has been of enormous value to the plant science research community. The use of isogenic material as a reference point or control is critical for many types of experiments in plant molecular biology and genetics. Recently, we noticed that some seed from a common source of the widely used Columbia-0 (Col-0) strain gave rise to plants showing features atypical for this strain. Whole-genome DNA-sequencing and allelespecific PCR assays confirmed that the abnormal individuals contain multiple introgressions from the ecotype Wassilewskija-2 (Ws-2), as described below. This …

Nebline, March 2016

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: It’s Easier Than Ever to Connect with Extension — Mobile Device and Social Media are Changing the Way People Learn

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Student-Conducted Farmer Video Interviews, Jenn K. Simons, Justin Van Wart, Charles Francis, Kristyn Harms

Department of Agronomy and Horticulture: Faculty Publications

High school agricultural education teachers have expressed concern about the lack of easily accessible educational materials dealing with contemporary topics in sustainable agriculture. There are numerous textbooks and monographs available for farmers and students at the college level, including the highly practical resources available from the Sustainable Agriculture Research and Education (SARE) book series on soil fertility (Magdoff and van Es, 2010), cover crops (Bowman et al., 2007) and building a farm business (DiGiacomo et al., 2003), among others. Although these are full of color photos and easily accessible graphs and tables, they are still in the print media category. …

Arkansas Cotton Variety Test 2015, Fred Bourland, A. Beach, C. Kennedy, L. Martin, A. Rouse, B. Robertson

Arkansas Agricultural Experiment Station Research Series

The primary goal of the Arkansas Cotton Variety Test is to provide unbiased data regarding the agronomic performance of cotton varieties and advanced breeding lines in the major cotton-growing areas of Arkansas. This information helps seed companies establish marketing strategies and assists producers in choosing varieties to plant. These annual evaluations will then facilitate the inclusion of new, improved genetic material in Arkansas cotton production.

A Next-Generation Marker Genotyping Platform (Ampseq) In Heterozygous Crops: A Case Study For Marker-Assisted Selection In Grapevine, Shanshan Yang, Jonathan Fresnedo-Ramírez, Minghui Wang, Linda Cote, Peter Schweitzer, Paola Barba, Elizabeth M. Takacs, Matthew Clark, James Luby, David C. Manns, Gavin Sacks, Anna Katharine Mansfield, Jason Londo, Anne Fennell Dr, David Gadoury, Bruce Reisch, Lance Cadle-Davidson, Qi Sun

Agronomy, Horticulture and Plant Science Faculty Publications

Marker-assisted selection (MAS) is often employed in crop breeding programs to accelerate and enhance cultivar development, via selection during the juvenile phase and parental selection prior to crossing. Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery. To bridge the gap between marker development and MAS implementation, this study developed a novel practical strategy with a semi-automated pipeline that incorporates traitassociated single nucleotide polymorphism marker discovery, low-cost genotyping through amplicon sequencing (AmpSeq) and decision making. The results document the development of a MAS package derived from genotyping-by-sequencing using three traits (flower sex, disease resistance and …

Nebline, February 2016

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Tips to Keep Your Heart Healthy — February is Heart Health Month

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Review Of Land Capability Assessment For The Swan Valley, Peter Tille, Angela Stuart-Street

Resource management technical reports

Land capability review for the Swan Valley.

The land capability review of the Swan Valley examined and updated previous soil and capability studies done in the area, which are still considered highly relevant for the planning requirements of the Swan Valley. As a result we have updated information on the potential for irrigated agriculture in the Swan Valley which will guide decision-making in the future.

This review uses a modified version of a new approach to presenting information for land use planning, developed by DAFWA in the Mid West region.

The main findings of the review include:

• The alluvial terraces …

The Folklore Of Flowers, Lisa Karen Miller

DLPS Faculty Publications

This presentation was prepared for WKU's Society for Lifelong Learning. It relates history and folklore of a variety of flowers, including their use in folk medicine, as food, and other stories and beliefs.

Corn Era Hybrid Response To Nitrogen Fertilization, Krishna P. Woli, Matthew J. Boyer, Roger Wesley Elmore, John E. Sawyer, Lori J. Abendroth, Daniel W. Barker

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) N use is of continued interest due to agronomic performance and environmental issues. This 2-yr study evaluated era hybrid response to fertilizer nitrogen (FN) rate in a factorial arrangement of one popular hybrid per five decades (1960–2000 eras) and five N rates (0–224 kg N ha^–1). An additional hybrid per era was grown at 168 kg N ha^–1. Hybrid productivity and nitrogen use efficiency (NUE) increased across the eras, but not between the 1980 and 1990 eras. Grain yield (GY) increased 65% and total plant biomass 43%, however, total plant nitrogen …

Frost Cycling And Irrigation, Peter Jeranyama, Faith Ndlovu, Casey Kennedy, Carolyn J. Demoranville

Cranberry Station Extension meetings

No abstract provided.

Fruit Rot Management, Erika Saalau Rojas

Cranberry Station Extension meetings

No abstract provided.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Midwest Vegetable Trial Reports

This paper reports on thirteen bicolor, two yellow, and three white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Sugar-Enhanced And Synergistic Sweet Corn Evaluations In Central Kentucky, Chris Smigell, John Strang, John Snyder

Midwest Vegetable Trial Reports

No abstract provided.

Second Year Results Using Biochar As A Soil Amendment In A High Tunnel, Polybag Growth System, Ron Goldy, Carly Andres, Virginia Wendzel

Midwest Vegetable Trial Reports

No abstract provided.

Pumpkin Cultivar Performance Trial Grown In Southern Ohio — 2015, Brad R. Bergefurd, Wayne Lewis, Thom Harker, Dane Peck, Dannah Diedrick

Midwest Vegetable Trial Reports

No abstract provided.

Winter Squash Variety Evaluation, John Strang, Chris Smigell, John Snyder, Pam Sigler

Midwest Vegetable Trial Reports

No abstract provided.

Seedless Watermelon Variety Trial For Kentucky, 2015, Shubin K. Saha, John Snyder, Chris Smigell, John Walsh

Midwest Vegetable Trial Reports

No abstract provided.

Chloroplast Rna-Binding Protein Rbd1 Promotes Chilling Tolerance Through 23s Rrna Processing In Arabidopsis, Shuai Wang, Ge Bai, Shu Wang, Leiyun Yang, Fen Yang, Yi Wang, Jiankang Zhu, Jian Hua

Department of Horticulture & Landscape Architecture Faculty Publications

Plants have varying abilities to tolerate chilling (low but not freezing temperatures), and it is largely unknown how plants such as Arabidopsis thaliana achieve chilling tolerance. Here, we describe a genome-wide screen for genes important for chilling tolerance by their putative knockout mutants in Arabidopsis thaliana. Out of 11,000 T-DNA insertion mutant lines representing half of the genome, 54 lines associated with disruption of 49 genes had a drastic chilling sensitive phenotype. Sixteen of these genes encode proteins with chloroplast localization, suggesting a critical role of chloroplast function in chilling tolerance. Study of one of these proteins RBD1 with an …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Purdue Fruit and Vegetable Research Reports

This paper reports on seven bicolor and two yellow sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Purdue Fruit and Vegetable Research Reports

This paper reports on thirteen bicolor, two yellow, and three white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Mini- And Heirloom Sweet Pepper Variety Performance In High Tunnels, 2015, Elizabeth Maynard, Israel S. Calsoyas

Purdue Fruit and Vegetable Research Reports

Trials on mini sweet peppers and other specialty sweet peppers in the Midwest have not recently been reported. We undertook this project to compare yield and fruit characteristics of hybrid and heirloom peppers grown in high tunnels using organic and conventional production methods.