Life Sciences Commons^™

Heterochronic Mutants Of The Nematode Caenorhabditis Elegans, Victor Ambros, R. Horvitz

Victor R. Ambros

Mutations in the Caenorhabditis elegans genes lin-14, lin-28, and lin-29 cause heterochronic developmental defects: the timing of specific developmental events in several tissues is altered relative to the timing of events in other tissues. These defects result from temporal transformations in the fates of specific cells, that is, certain cells express fates normally expressed by cells generated at other developmental stages. The identification and characterization of genes that can be mutated to cause heterochrony support the proposal that heterochrony is a mechanism for phylogenetic change and suggest cellular and genetic bases for heterochronic variation.

Explorations, Vol. 1, No. 1, R. D. Blake, Katherine Carter, David Mackinnon Ebitz, Carole J. Bombard, Stephen A. Norton

Explorations — A Journal of Research

Welcome to the first issue of Explorations, A Journal of Research at the University of Maine at Orono.

Join us as we explore a representative selection of the pure and applied research of our faculty. At UMO, we believe that research can capture the imagination and invigorate the mind, as well as contribute directly to the quality of life of the citizens we serve.

In this first issue, we have selected four areas of research that span the disciplines of biological and environmental sciences and the arts. This is but a small part of the research conducted by the faculty …

Expression Of Epstein-Barr Virus (Ebv) Dna And Cloned Dna Fragments In Human Lymphocytes Following Sendai Virus Envelope-Mediated Gene Transfer, David J. Volsky, Thomas Gross, Faruk Sinangil, Charles Kuszynski, Ronald Bartzatt, Timothy Dambaugh, Elliott Kieff

Chemistry Faculty Proceedings & Presentations

Purified EBV DNA and cloned DNA fragments were trapped in Sendai virus (SV) envelopes during envelope reconstitution. The DNA-loaded reconstituted envelopes (RSVE/DNA) served as gene-transfer vehicles using the capability of RSVE to fuse with normal and tumor cells. The efficiency of RSVE-mediated EBV DNA transfer into lymphoid tumor cells and fresh human lymphocytes was 5-10% of the enveloped 3H-labeled EcoRI fragment B of EBV DNA. Purified intracellular EBV (B95-8 strain) DNA induced EBV nuclear antigen (EBNA) in 0.2-1% of human lymphocytes, transiently stimulated cellular DNA synthesis, but did not fully transform cells. Cloned Sal I F1 fragment [approximately equal to …

Molecular Genetic Characterization Of The Rd-114 Gene Family Of Endogenous Feline Retroviral Sequences, Roger H. Reeves, Stephen J. O'Brien

Biology Faculty Articles

RD-114 is a replication-competent, xenotropic retrovirus which is homologous to a family of moderately repetitive DNA sequences present at ca. 20 copies in the normal cellular genome of domestic cats. To examine the extent and character of genomic divergence of the RD-114 gene family as well as to assess their positional association within the cat genome, we have prepared a series of molecular clones of endogenous RD-114 DNA segments from a genomic library of cat cellular DNA. Their restriction endonuclease maps were compared with each other as well as to that of the prototype-inducible RD-114 which was molecularly cloned from …

Isolation Of A Collagenase Cdna Clone And Measurement Of Changing Collagenase Mrna Levels During Induction In Rabbit Synovial Fibroblasts., Robert H. Gross, Lynn A. Sheldon, Colin F. Fletcher, Constance E. Brinckerhoff

Dartmouth Scholarship

To facilitate our studies on the mechanisms controlling collagenase production at a molecular level in rabbit synovial fibroblasts, we have constructed a cDNA library using mRNAs isolated from cells induced with crystals of monosodium urate monohydrate. We have screened this library with cDNA probes made from induced and control mRNA populations. From among 30 clones that hybridized preferentially to the induced-cell probe, 4 contained collagenase sequences. The largest, a clone of 650 base pairs, was identified by its ability to hybrid select a mRNA that could be translated in a cell-free system into a product that was precipitable with monospecific …

Growth, Yield, And Enfor, Imre Bella

Aspen Bibliography

Increasing public awareness about the state of forestry and concerns about present and future wood supplies are bringing about an intensification of forest management across the country.

Ultraviolet Light Reactivation Of Gamma-Ray Induces Chromosome Aberrations In G1 Phase Xenopus Cells, Susan Kulp, Linda Rogers, H. Gaston Griggs

Journal of the Arkansas Academy of Science

No abstract provided.

The Frequency Of The Fragile-X Chromosome In Twenty-Three Autistics From Eastern Virginia, Jennifer Morris Ferguson

Dissertations, Theses, and Masters Projects

No abstract provided.