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Genetics and Genomics

Clemson University

2008

ADF9

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Identifying The Cis-Acting Sugar Response Elements That Facilitate Glucose Signaling In Arabidopsis Thaliana, Frank Barretta, Jr. Dec 2008

Identifying The Cis-Acting Sugar Response Elements That Facilitate Glucose Signaling In Arabidopsis Thaliana, Frank Barretta, Jr.

All Theses

Identifying the initial sugar sensing and response activating mechanisms in plants has been difficult due to the dual functions of sugars as nutrients and as signaling molecules. In this study, we have examined transcript expression and promoter features of different genes encoding proteins for actin remodeling that have been implicated as targets of plant glucose signaling. Using Reverse Transcription PCR analysis, we confirmed that expression of two actin-associated genes, actin depolymerizing factor 9 (ADF9) and actin related protein 8 (ARP8), are repressed following a short-term glucose treatment of Arabidopsis thaliana seedlings. Glucose did not repress the expression of other ADF …