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Full-Text Articles in Life Sciences
Identifying The Cis-Acting Sugar Response Elements That Facilitate Glucose Signaling In Arabidopsis Thaliana, Frank Barretta, Jr.
Identifying The Cis-Acting Sugar Response Elements That Facilitate Glucose Signaling In Arabidopsis Thaliana, Frank Barretta, Jr.
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Identifying the initial sugar sensing and response activating mechanisms in plants has been difficult due to the dual functions of sugars as nutrients and as signaling molecules. In this study, we have examined transcript expression and promoter features of different genes encoding proteins for actin remodeling that have been implicated as targets of plant glucose signaling. Using Reverse Transcription PCR analysis, we confirmed that expression of two actin-associated genes, actin depolymerizing factor 9 (ADF9) and actin related protein 8 (ARP8), are repressed following a short-term glucose treatment of Arabidopsis thaliana seedlings. Glucose did not repress the expression of other ADF …
Utilizing Multiplex Ligation-Dependent Probe Amplification To Detect Novel X-Linked Microduplications Which Cause Intellectual Disability, Dianne Cohn
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ABSTRACT
A number of conditions related to X-linked intellectual disabilities (XLID) are in part due to microduplications that are not visible cytogenetically. With the focus on Rho, Ras and Rab genes, a family of genes known to be associated with intellectual disabilities, were screened for dosage aberrations (Leeuwen, F. N. 1997), (Ng, E. L. 2008), (Gissen, P. 2007), (Gurkan, C. 2005). Cohorts of intellectually disabled ID individuals were explored with new technologies. These new technologies include comparative genomic hybridization (CGH), multiplex ligation dependent probe amplification (MLPA) and quantitative PCR (qPCR) (Madrigal, I. 2007), (Hermsen, M. A. 2005), (Morey, J. S. …
Functional Characterization Of Hexokinase-Like 1 (Hkl1) From Arabidopsis Thaliana, Abhijit Karve
Functional Characterization Of Hexokinase-Like 1 (Hkl1) From Arabidopsis Thaliana, Abhijit Karve
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Arabidopsis hexokinase1 (AtHXK1) is a moonlighting protein with roles in both glucose signaling and catalysis. In this study, we first cloned and characterized the six HXK related genes from Arabidopsis. Three of the six encoded proteins were shown not to phosphorylate hexoses and thus, are designated as hexokinase-like (HKL) proteins. Though they are only 50% identical to HXK1, the amino acid sequences of HKL1 and HKL2 both have well conserved glucose binding domains and other recognized structural elements. The possible basis for their lack of catalytic activity was further probed by site-directed mutagenesis and ultimately was attributed to a suite …
Insight Into The Origins Of The Rumex Acetosa Sex Chromosomes And Mechanisms Causing Dimorphism, Samuel Forrest
Insight Into The Origins Of The Rumex Acetosa Sex Chromosomes And Mechanisms Causing Dimorphism, Samuel Forrest
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Sex conferring chromosomes are subject to rapid change throughout evolution. Y-chromosomes (male determining) are subject to extensive chromatin remodeling and higher mutation rates than the autosomes being that recombination with the X chromosome is inhibited in many regions. The non-recombining regions comprise varying proportions of Y chromosomes from species to species and they are subject to mutation acquisition including both deletions and insertions. Recently it has been shown that these insertion mutations include repetitive DNA such as satellite DNA sequences (satDNA) and transposable elements. It is thought that Y chromosomes go through many rounds of expansion and contraction throughout evolution …
Dna Methylation And Promoter Sequence Analysis Of Colon Cancer Genes, Fang Wang
Dna Methylation And Promoter Sequence Analysis Of Colon Cancer Genes, Fang Wang
All Theses
Aberrant DNA methylation in the genome is associated with human cancers. Tumor cells can undergo an overall loss of DNA methylation in non-coding repetitive regions (including the Alu elements) and at the same time maintain hypermethylation in the CpG islands in promoter region of multiple genes. We are interested in understanding the pattern of DNA methylation in the promoters of genes that may mediate the tumor genesis in colon cells. Recent literature survey suggested that about two dozens of genes, when mutated or undergone changes in DNA methylation, can directly promote the development of colon cancers. We downloaded the promoter …