Life Sciences Commons^™

The Use Of Microbiome Sequencing To Identify Individuals In Forensic Science, Sophia Konieczny

Themis: Research Journal of Justice Studies and Forensic Science

The Human Genome Project's (HGP) completion in 2003 laid the groundwork for further research into the human body. This paved the way for microbiome sequencing, revealing the diverse microbial communities within the human body. These advancements led to the emergence of microbial forensics, leveraging Next Generation Sequencing (NGS) technologies like Amplicon sequencing and shotgun metagenomics for individual identification. Unlike traditional genetic profiling, microbiome analysis provides insights beyond genetic profiling, offering information about an individual's lifestyle and environment. Microbial communities on the skin's surface and objects can serve as trace evidence, aiding in suspect identification and investigative leads. Despite its promise, …

Coomassie Brilliant Blue Dye As A Method For Analyzing Fracture Markings In Bone, Abigail Hoffmeister, David Harutunyan, Matthew Aizawa, Everett Baker, Brandon Mendoza, Chase Freeman, Siran Iskanian

Seaver College Research And Scholarly Achievement Symposium

Coomassie Brilliant Blue Dye is a dye commonly used to stain proteins. Because of its ability to adhere to proteins, this research has focused on perfecting a method of dyeing a fractured flat bone in order to most accurately observe and analyze fracture markings within the trabecular layer. Stereoscopic microscopy was the chosen technique of analysis for this research because of its proven effectiveness in glass and ceramic fractography to observe varying depths. In order to most effectively apply stereoscopic microscopy to this research, the following variables were manipulated to maximize color contrast in the trabecular layer in order to …

2023 Ulm Student Research Symposium Proceedings, Burton Ashworth

Multidisciplinary Psychology: A Journal of Collaboration

This is the results and proceedings from the 2023 ULM Student Research Symposium.

Extraction Of Challenging Forensic Samples Using The Microgem Dna Extraction Kit, Falyn R. Vega

Student Theses

In forensic science, DNA extraction can be a tedious and resource-intensive process. Extraction with Proteinase K is an industry standard but has its drawbacks, such as requiring multiple ionic detergents and washing steps. MicroGEM has developed a new enzyme called forensicGEM that is temperature-dependent and compatible with mesophilic enzymes, offering complete DNA extraction in about 20 minutes in a single tube, limiting contamination, loss of sample, and working time. ForensicGEM can extract DNA from highly degraded samples, potentially leading to more complete STR profiles. Highly degraded tissue and bone samples were collected and extracted with the forensicGEM …

Structural Integrity And Stability Of Dna In Ionic Liquid And Near-Infrared Indolizine Squaraine Dye, Ember Yeji Suh

Honors Theses

Luminol, the most common presumptive test for blood at a crime scene, has multiple issues, such as false positive results with chemical agents, no luminescence due to “active oxygen” cleaning agents on bloodstains, and inability to penetrate textile materials. A combination of indolizine squaraine dye and ionic liquid (IL), or Dye Enhanced Textile Emission for Crime Tracking (DETECT), have shown potential to address these issues. The purpose of this study was to assess the binding mechanism of CG (1:1) and SO₃SQ dye to HSA and how the mechanism can explain the W214 fluorescence quenching effect and to determine …

Trace Dna Detection Using Diamond Dye: A Recovery Technique To Yield More Dna, Leah Davis

Master's Theses

This study aspires to find a new screening approach to trace DNA recovery techniques to yield a higher quantity of trace DNA from larger items of evidence. It takes the path of visualizing trace DNA on items of evidence with potential DNA so analysts can swab a more localized area rather than attempting to recover trace DNA through the general swabbing technique currently used for trace DNA recovery. The first and second parts consisted of observing trace DNA interaction with Diamond Dye on porous and non-porous surfaces.

The third part involved applying the Diamond Dye solution by spraying it onto …

Dna Evidence: The Forensic Tool That Provides Both Accuracy And A Violation Of Privacy, Garrett Malove

Student Writing

DNA Forensics, the use of DNA by law enforcement in investigations, has been the most effective tool for solving crimes in human history. Through the over 90% accuracy it provides, it has caught repeat offenders, solved cold cases sooner, and exonerated innocent individuals. However, throughout the 2010s, especially after the Maryland v. King decision in 2013 which mandated the collection of DNA from individuals arrested for felonies, privacy concerns have been raised not just for the arrestees themselves, but for the current free access law enforcement has to DNA databases. Those who support the status quo, being the idea that …

Dna Transfer And Persistence On Non-Porous Surfaces Submerged In Spring Water [Preliminary Study], Morgan L.. Korzik, Josep De Alcaraz-Fossoul, Michael S. Adamowicz, David San Pietro

Forensic Science: Faculty and Staff Publications

Submerged items are often thought to lack evidentiary value. However, previous studies have shown the ability to recover DNA from submerged porous items for upwards of six weeks. The crevices or interweaving fibers in porous items are thought to protect DNA from being washed away. It is hypothesized that, because non-porous surfaces do not have the same traits that might aid in DNA retention, then DNA quantities and the number of donor alleles recovered would decrease over longer submersion periods. Additionally, it is hypothesized that DNA quantity and the number of alleles would be negatively affected by flow conditions. Neat …

Elisa Validation Method For The Detection Of Ketamine In Hair, Daria A. Centonza

Student Theses

An ELISA method was developed and validated to detect ketamine in human hair samples. Ketamine is an anesthetic drug that causes memory loss, dissociative sensations, and hallucinations. Due to these adverse effects, ketamine is a common drug used in drug facilitated sexual assaults (DFSAs). It is very important to be able to detect the substances used in DFSAs over a longer period of time due to the delayed reporting of these crimes. Victims, often out of fear and from the sedative/memory loss effects of the drugs, tend to report these crimes when it is too late to use urine and …

Bci Validation: Yfiler Plus Kit Validation, Chloe Koon

Honors Projects

In order to have access and use the Combined DNA Index System (CODIS), it is required by the Federal Bureau of Investigation's Quality Assurance Standards (FBI-QAS) for Forensic DNA Units to complete comprehensive validation studies on all equipment, materials, and methods used in the process of DNA analysis. This study continues validation previous done within the Ohio Bureau of Criminal Investigation (BCI). YFiler Plus is a DNA amplification kit that is used in cases where it is most useful to examine only male DNA profile(s) within a sample by amplifying loci only on the Y-Chromosome. Components of this study include …

Scientific Development Of An Integrated Workflow For Latent Print, Questioned Document, And Dna Processing Of Paper Evidence, Ashley Morgan

Dissertations, Theses, and Capstone Projects

Touch paper evidence could be the source of probative human DNA but recovery is challenging and forensic laboratories instead prioritize processing by the Latent Print and Questioned Document disciplines. Recent advances in DNA collection methods and the increased sensitivity of STR typing kits have improved success rates for DNA testing of paper evidence; but prior to implementing DNA collection, laboratories have to decide in which order to examine paper for the different types of forensic evidence. This thesis developed and tested a multi-discipline workflow for processing paper evidence by DNA, Latent Prints and Questioned Documents experts. Preliminary sampling studies indicated …

Complex Dna Mixture Deconvolution By Single Cell Genomics: Direct Physical Cell Subsampling And High Sensitivity Dna Typing, Kaitlin Huffman

Electronic Theses and Dissertations, 2020-

Biological evidence is often found at crime scenes and can comprise of DNA from the victim(s) and perpetrator(s) to a crime as well as from individuals with no direct relationship to the incident. This can complicate analysis as DNA mixtures are one of the more difficult sources of biological evidence to interpret. Probabilistic genotyping (PG) has greatly aided in mixture analysis. However, even with PG, standard bulk mixture approaches do not always result in probative results as allele overlap, artifacts, or low-level minor contributors inevitably cause genotype information loss. Therefore, deconvolution of forensic DNA mixtures into their individual component DNA …

Analyzing The Viability Of Direct Pcr For Use In Conjunction With Cyanoacrylate Enhanced Fingerprints, Coral M. Smith

Graduate Theses, Dissertations, and Problem Reports

Efficient methods for DNA analysis are desperately needed in laboratories due to the influx in DNA analysis requests. The current DNA processing methods are costly, time consuming, and involve multiple tube changes, increasing the risk of contamination. However, the use of direct PCR can simplify the DNA analysis process by eliminating the extraction, purification and quantitation steps. Presently, the FBI Quality Assurance Standard 9.4 requires all evidence samples to be quantitated. As a result, the direct PCR method of DNA processing cannot be implemented on evidence samples at this time. Direct PCR presents the opportunity to provide efficient DNA analysis, …

Mtdna Heteroplasmy In Hair Shafts Versus Buccal Swabs For Forensic Applications, Sara R. Bodnar

Graduate Theses, Dissertations, and Problem Reports

Mitochondrial DNA (mtDNA) analysis has several forensic applications such as criminal investigations, identification of human remains, and missing person investigations. It is also the only type of DNA that is available from certain sample types such as hair shafts. The presence of mtDNA heteroplasmy within and between tissue types can lead to mtDNA sequence differences when comparing samples originating from the same individual. Studies on mtDNA heteroplasmy are increasingly being carried out for their implications in forensic interpretation of mtDNA sequences. Specifically, mtDNA in buccal swabs compared to hair samples from one individual may show differences in sequence due to …

Identification Of The New Synthetic Opioid Bucinnazine And Its Metabolites Using Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Karissa N. Resnik

Master of Science in Forensic Science Directed Research Projects

In the past 20 years, the rate of opioid use increased drastically in the United States. The country has experienced an enormous number of deaths caused by opioid overdose, opioid use disorder (OUD), as well as other harms as a consequence of the high numbers of opioid prescriptions. Fentanyl, a well-known synthetic opioid, has been a contributor to the opioid crisis since 2013 due to its popularity in clinical and illicit use. It has a high potency, which makes it an effective analgesic but a dangerous illicit substance. A new synthetic opioid, bucinnazine, has recently become a new black-market opioid …

Death Scene Insect Succession In Nebraska: A Guidebook, Erin Bauer, Larry Barksdale, Emma Sidel, Justine Laviolette

Department of Entomology: Faculty Publications

Insect behavior can be helpful to law enforcement in determining time of death, manner of death, location, and environment related to human or other animal victims found at a death scene. They may also provide clues about other aspects associated with an investigation (i.e., fly specks, suspect DNA). The study of how insects and related arthropods can aid in legal investigations is known as forensic entomology. Although this includes both civil applications, such as urban (i.e., maggots in mortuaries or insect structural damage) or stored product (i.e., illness from food contamination) entomology, this manual focuses on criminal applications, such as …

Reproducibility Of Individual Dna Deposits Detected Through Cellular Fluorescence, Natalee Small-Davidson

Student Theses

Contact traces are an important part of DNA casework, but the probative value of any identified associations depends on the possibility of passive transfer. There is known individual variation in DNA left behind during contact, this DNA shedding propensity has an effect on whose DNA is detected. This study evaluated this variability using a cell staining approach. Volunteers were asked to deposit a fingerprint on a clean glass slide, then wash their hands and deposit a second fingerprint after a 30-minute wait without touching anything. Three sets of samples were collected over three consecutive weeks. Fingerprints were stained with a …

Evaluating A Test For Shedding Propensity Using Tape Lifts From Different Skin Locations, Xiao M. Chen

Student Theses

The shedding propensity of a person can assist data interpretation in casework when assessing the possibility of passive transfer for DNA analysis. Past studies on shedding propensity evaluated palmar skin (washed and unwashed) deposits. This study compared different skin locations with respect to shedding propensity, and explored the potential of tape-lifts as a skin surface collection method. Eight different skin types and samples were collected with adhesive tape disks from 28 participants over three non-consecutive days; the washed and unwashed fingers from both hands, toe, and arm, neck below ear, and nape. Samples were extracted, quantified, amplified, genotyped, and evaluated …

Evaluation Of The Trunarc Handheld Narcotics Analyzer As A Pre-Analysis Screening Device For The Orange County Crime Lab, Sarah Yang, D. Bauer, C. Woltz, S. Soto, Michael Ibba

Student Scholar Symposium Abstracts and Posters

Forensic analysis of suspected narcotics is often dangerous as the substances’ composition is unknown. Many techniques for drug identification require handling of the substance outside of its packaging, which can expose the analyst to potentially harmful chemicals. The TruNarc Handheld Narcotics Analyzer is a portable Raman spectroscopy device that is non-destructive of evidence and can be used to screen drugs through simple packaging to minimize the risk of exposure. The Orange County Crime Lab (OCCL) is testing the limits of this device to determine if it can be used to screen new evidence within the Seized Drugs Lab. The OCCL …

Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner, Jada T. Alexis

Student Theses

Cocaine overdose deaths are on the rise in the United States. This increase may be due to the co-consumption of cocaine with other drugs. When cocaine is ingested in conjunction with alcohol, a specific metabolite called cocaethylene forms. Information regarding cocaethylene, specifically its interpretive value in post-mortem cases, has not been widely studied. Data from the Office of the Chief Medical Examiner in New York City revealed that there were 195 cases in 2017 positive for cocaethylene. These cases were most likely to involve black (42.1%) males (79.5%) in Manhattan (28.2%) aged 50 - 59 (34.4%). Most of the cases …

Evaluation Of The Effect Of The Humic Acid Inhibitor On Forensic Genetic Investigations Of Human Skeletal Remains, Madeline King

Honors Theses

Postmortem survival of DNA in human skeletal remains occurs due to the compact microstructure of the skeleton and its ability to provide a strong, protective physical barrier to environmental insults. On a molecular level, DNA preservation in bones/teeth involves electrostatic interactions between the negatively-charged DNA backbone and positively charged calcium residues in hydroxyapatite, the latter of which is one of the main components of bone microstructure. Despite these protections, over time endogenous DNA becomes damaged, limiting our ability to detect it and affecting its utility in making a positive identification. Hence, forensic genetic investigations of unidentified human remains (UHRs) are …

Extraction Of Human Dna From Soil In A Simulated Clandestine Grave, Arely Joaly Parra Lopez

Honors Theses

Locard’s Exchange Principle states that “every contact leaves a trace.” The same principle applies when a perpetrator of a homicide decides to bury a victim in a clandestine grave. If a perpetrator originally buried a murder victim in haste to prevent discovery and then decided to later move the victim’s body to a remote location for disposal, decomposition fluids from the victim’s body (which contain cellular material and therefore DNA) would remain in the surrounding grave soil at the original burial site. It is possible that investigators could: 1) prove that a human body had once laid in that location …

Determination Of Human Shedding Propensity Based On Str Results, Genevieve Trapani

Student Theses

Trace DNA evidence may be discovered at a crime scene after having been deposited by a person of interest via active or passive transfer. Based on previous studies, passive transfer of one’s DNA is influenced by their shedding propensity, or probability of depositing a detectable amount of DNA through touch. Determining the shedding propensity of a person of interest can aid in trace DNA interpretation in forensic casework. This study explored STR profile quality and the presence of a DNA mixture for different skin surface locations, including fingertips before and after handwashing. As expected, unwashed fingers showed a higher prevalence …

How To Get Away With Murder: The Effectiveness Of Blood Detectors On Surfaces Cleaned Of Blood, Grace Cavanaugh

Undergraduate Theses and Capstone Projects

The purpose of this experiment was to test blood detectors against household cleaners. Similar experiments have been run before, but not on the selected surfaces. It is important to understand what one is looking for at a crime scene, and what a cleaned surface may look like. Four surfaces were chosen for this experiment: ceramic tile as a control, concrete, laminated wood flooring, and drywall. For each surface, 0.5mL of pig’s blood was allowed to dry before being cleaned off with either Clorox bleach, Dawn soap and hot water, a Mr. Clean Magic Eraser, or Mrs. Meyer’s Multi-Purpose Concentrate. Bluestar …

Pornography: Social, Emotional And Mental Implications Among Adolescents, William Kelly Canady

National Youth Advocacy and Resilience Conference

This presentation will explain the historical development of pornography. It will highlight four segments: 1- Porn’s impact on brain development of reward pathways, ultimately increasing the appetite for more porn. 2- Porn can be a false substitute for real intimacy, resulting in decreased sexual satisfaction with a real person and increased verbal and physical aggression. 3- Porn promotes sex trafficking, promotes multiple sex partners and reduced STD prevention. 4- A review of interventions available to assist clients in navigating a lifestyle away from pornography.

Forensic Entomology: Insect Succession – Northern Colorado, Thomas Nissen

Department of Entomology: Distance Master of Science Projects

Forensic entomology is considered a relatively new field of entomology yet insects have been around for millions of years. Insects have been associated with dead animals and humans for centuries. The remains of insects have been recorded from Ancient Egypt to the present time. Recent advancements have been with data presented to the courts that show that there is a specific order to insects arriving on a corpse and that order helps to determine time of death. This study investigates the order of insect succession in the northern front range area of Colorado.

Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake

Student Theses

Effective DNA extraction methods are important for forensic applications. The main goal of this experiment was to determine if a newly developed trypsin based protein/DNA co-extraction method applied to contact traces would yield comparable results to a commercial Proteinase K method (QIAamp DNA Investigator Kit) and a Chelex extraction method with Tween 20 used in forensic laboratories. This was tested on 20 sets of sebaceous fingerprints on glass slides.

The results of this study demonstrate the trypsin co-extraction method yielded the highest amount of DNA. In the first comparison, the mean total DNA yields for the trypsin-co extraction method and …

Further Evaluation Of A Dry Vacuuming Technique For Recovery Of Dna From Handwritten Documents, Christian Hopkins

Student Theses

A previously developed, non-destructive, homemade vacuum method for collecting biological material from handwritten documents had promising DNA results for white copy paper, preserved indented writing, and latent prints. Prior to casework implementation, additional validation experiments are warranted and here the method was tested for different paper substrates. This work describes testing of notebook paper, bank deposit slips, magazine pages, and manila envelopes. The quantity of recovered DNA varied from donor to donor, but the mean quantities showed a trend that can be explained by the different sizes and surface properties of the tested paper types. The rougher paper type, like …

Investigating The Efficacy Of Dna Damage With Bleach In Forensic Laboratories And At Crime Scenes, Alyssa Tuccinardi

Honors Theses

Household/commercial bleach (6% NaOCl, sodium hypochlorite) degrades DNA through oxidative damage, production of chlorinated base products, and cleavage of DNA strands (breaking it into smaller and smaller fragments). The presence of these lesions significantly impacts the ability to generate a full genetic profile from an evidentiary sample. In fact, knowledge of the damaging effect of bleach on DNA is the basis for its use in forensic laboratories to clean workbenches and prevent cross-contamination of samples between cases. Additionally, bleach is used intentionally by criminals to clean up crime scenes and destroy DNA evidence. A previous study demonstrated that bleach has …

Current Applications And Limitations Of Forensic Entomology, Helena Volckaert

Themis: Research Journal of Justice Studies and Forensic Science

Forensic entomology is the study of arthropods concerning their application in crime scene investigation as a method of predicting postmortem interval (PMI) in deceased or missing individuals. PMI is determined by the life stage of insects present on-scene. Insects used in criminal investigations are primarily beetles, flies, or moths; this may also include various scavenging insects and hitchhiking mites. These creatures help colonize decayed tissue, providing crime scene personnel with live specimens collected and examined for biological evidence. Forensic entomology is currently a niche application in most investigations—as qualified entomologists are often needed—and proper analysis of collected evidence can be …