Life Sciences Commons^™

Perchlorate And Nitrate Remediation Efficiency And Microbial Diversity In A Containerized Wetland Bioreactor, Paula Krauter, Bill Daily Jr., Valerie Dibley, Holly Pinkart, Tina Legler

All Faculty Scholarship for the College of the Sciences

We have developed a method to remove perchlorate (14–27 μg/L) and nitrate (48 mg/L) from contaminated groundwater using a wetland bioreactor. The bioreactor has operated continuously in a remote field location for more than 2 yr with a stable ecosystem of indigenous organisms. This study assesses the bioreactor for long-term perchlorate and nitrate remediation by evaluating influent and effluent groundwater for oxidation-reduction conditions and nitrate and perchlorate concentrations. Total community DNA was extracted and purified from 10-g sediment samples retrieved from vertical coring of the bioreactor during winter. Analysis by denaturing gradient gel electrophoresis of short, 16S rDNA, polymerase-chain-reaction products …