Life Sciences Commons^™

Monitoring Whitebark Pine Stand Health In The Central Washington Cascades, Nancy H. Parra, Teresa J. Lorenz, Taza D. Schaming, Alison Scoville

Student Published Works

Whitebark pine (Pinus albicaulis) plays a vital role in colonizing newly disturbed areas, providing shade for other tree species to germinate, and supplying food for a variety of birds and mammals, such as Clark’s Nutcrackers (Nucifraga columbiana) and grizzly bears (Ursus arctos horribilis). Decline of whitebark pine populations has been attributed to several factors, including white pine blister rust (Cronartium ribicola), mountain pine beetle (Dendroctonus ponderosae) outbreaks, and fire exclusion. In 2009, the U.S. Forest Service began to install permanent plots in whitebark pine stands in Washington and Oregon as …

Wilderness And The Geotag: Exploring The Claim That "Geotagging Ruins Nature" In The Alpine Lakes Wilderness, Wa, Mara Gans

All Master's Theses

This research explores the claim that “geotagging ruins nature” by quantifying and qualifying patterns in geotag use and visitors’ experiences in the Alpine Lakes Wilderness, in Washington, United States. Many have raised concerns that geotags increase recreational visitation to public lands, which subsequently contributes to negative resource impacts. Others, however, claim that geotagging has made the outdoors more accessible to less privileged communities and raise concerns that condemning geotags will perpetuate the exclusion of certain groups from outdoor recreation. This debate is studied within federally designated Wilderness, which is legally defined as “untrammeled by man,” a definition rooted in problematic …

Habitat Characteristics Or Protected Area Size: What Is More Important For The Composition And Diversity Of Mammals In Nonprotected Areas?, Wenbo Li, Jinhua Li, Peipei Yang, Bowen Li, Chao Liu, Lixing Sun

Biology Faculty Scholarship

The margins of protected areas are usually considered to have greater forest degradation, and given that most mammals live outside protected areas, researchers and conservation practitioners are increasingly recognizing that nonprotected areas must be incorporated into conservation strategy. However, the strategy used to manage these areas still involves increasing the size of protected areas, while not considering the habitat characteristics and requirements of the species. In this study, during a 3-year period, camera trap and habitat characteristic surveys were used to estimate composition, diversity, and habitat characteristics of mammals to determine habitat characteristics or increase the size of protected areas …

Dynamic Microclimate Boundaries Across A Sharp Tropical Rainforest–Clearing Edge, Eric A. Graham, Mark Hansen, William J. Kaiser, Yeung Lam, Eric Yuen, Philip W. Rundel

Biology Faculty Scholarship

As landscapes become increasingly fragmented, research into impacts from disturbance and how edges affect vegetation and community structure has become more important. Descriptive studies on how microclimate changes across sharp transition zones have long existed in the literature and recently more attention has been focused on understanding the dynamic patterns of microclimate associated with forest edges. Increasing concern about forest fragmentation has led to new technologies for modeling forest microclimates. However, forest boundaries pose important challenges to not only microclimate modeling but also sampling regimes in order to capture the diurnal and seasonal dynamic aspects of microclimate along forest edges. …

An Ecological Comparison Between Resource Subsidies: Pacific Lamprey (Entosphenus Tridentatus) And Pacific Salmon (Oncorhynchus Spp.), Jocelyn Wensloff

All Master's Theses

Historically, oligotrophic Pacific Northwest (PNW) streams received annual returns of spawning anadromous fish that provided resource subsidies in the form of marine-derived nutrients (MDN), thus driving stream food web productivity. To date, many studies in the PNW have focused on Pacific salmon (Oncorhynchus spp.) as a resource subsidy, overlooking other anadromous fish species such as Pacific lamprey (Entosphenus tridentatus). Both Pacific salmon and Pacific lamprey are culturally important to PNW tribes for ceremonial, medicinal, and subsistence purposes, and have been since time immemorial. Unfortunately, both salmon and lamprey populations are in decline. Historically, lamprey have been disregarded …

Hot But Not Dry: Modest Changes In Water Relations For An Epiphytic Bromeliad In A Tropical Dry Deciduous Forest, Saúl Hernández-Robinson, Eric A. Graham, Olivia Hernández-González, Roberth Us-Santamaría, José Luis Simá, Fernando Arellano-Martín, José Luis Andrade

All Faculty Scholarship for the College of the Sciences

Premise of research. Epiphytic bromeliads endure intense seasonal environmental changes in the canopy of dry tropical deciduous forests. The analysis of the physiological responses of these epiphytes to environmental changes can be useful in assessing their plasticity, vulnerability, and adaptations to such extreme habitats.

Methodology. We measured microenvironmental variables and water relations for plants of the epiphytic bromeliad Tillandsia brachycaulos in three microhabitats within the canopy of a dry tropical forest. We measured individual plants for seasonal and spatial differences in light, leaf temperature, osmotic potential, cell wall elasticity, and relative capacitance as indications of their physiological responses …

Evaluation Of The Relationship Between Land Use And Water Quality In Kittitas County, Wa, Lindsay Schulz

All Master's Theses

Water in Kittitas County is extremely valuable since it supports farming, recreation, and cultural activities, as well as environmental processes and a diversity of biological life while providing many ecosystem services. However, land conversions required by agricultural and urban land uses can negatively impact water quality and the biological function of the stream. I studied how forested, agricultural, and urban land use affect six streams. Fourteen sites were sampled, once each in July, August, and September 2019. Land use was calculated as a percentage of forested, agricultural, and urban land use within a 100-m buffer of the stream, upstream of …

Sampling The Local Fare: Fishes At The Sam Israel House Pit (45gr76), Soap Lake, Washington, Adam Fruge

All Master's Theses

The Sam Israel site is a precontact archaeological complex with numerous fish bones at the north end of Soap Lake, Washington. Excavated in 1976, the fish remains recovered from there were never fully analyzed prior to this research. Since this inland Columbia Plateau site had thousands of fish bones, it contained untapped potential for our understanding of ancient local fish procurement. As such, I conducted a detailed analysis of 2,862 fish bone specimens from the Sam Israel House Pit locus to: study a larger sample of fish bones in greater detail than was done before; compare the distribution of fishes …

Distribution Of Invasive Plant Species On Debris Cones At Mount Rainier National Park, Shaun Morrison

All Master's Theses

Invasive plant populations within Mount Rainier National Park are a biological and ecological threat to the unique mountain landscape. A better understanding of their distribution and transport within the landscape is needed to improve invasive species monitoring for National Park Service management. This study investigates how invasive plant populations in Stevens Canyon are utilizing the debris cone disturbances and associated geomorphic processes to facilitate movement within the park. Vegetation transects were performed along Stevens Canyon Road (to observe the roadside community composition) and on the debris cone features (to observe species movement from the roadside). These vegetation observations are presented …

Holocene Fire History Reconstruction Of A Mid-Evaluation Mixed-Conifer Forest In The Eastern Cascades, Washington, Zoe Rushton

All Master's Theses

Fire histories of mid-elevation mixed-conifer forests (MEMC) are uncommon, particularly in the eastern Cascades of Washington. As a result, fire regimes and the effects of 20th century fire suppression in these forests are not well understood. In the summer of 2014 a 7.80 meter-long sediment core was extracted from Long Lake, located approximately 45 km west of Yakima, WA, which exists in a grand fir-dominated mixed-conifer forest. Fire activity for the Long Lake watershed was reconstructed using macroscopic charcoal analysis and pollen analysis was used to reconstruct vegetation change through time. Charcoal results show low fire activity in the early …

Ground-Truthing Social Vulnerability Indices Of Alaska Fishing Communities, Anna Lavoie, Kim Sparks, Stephen Kasperski, Amber Himes-Cornell, Kristin Hoelting, Conor Maguire

All Faculty Scholarship for the College of the Sciences

Community vulnerability is increasingly evaluated through quantitative social indices, typically developed using secondary data sources rather than primary data collection. It is necessary to understand the validity of these indices if they will be used to inform policy and decision making. This paper presents a ground-truthing effort to validate quantitative indices that characterize the well-being of Alaska fishing communities. We utilized ethnographic data collected from 13 representative communities and a capital assets framework to ground-truth the indices, in which qualitative ranks of vulnerability were compared against quantitative indices. The majority (73.8%) of ranks were in complete or moderate agreement and …

Intertidal Habitat Utilization By Endangered Green Sturgeon (Acipenser Medirostris), Willapa Bay, Washington, Luke Stilwater

All Master's Theses

This study looks at a portion of the designated critical habitat for the threatened southern distinct population segment of green sturgeon (Acipenser medirostris) in Willapa Bay, Washington. Willapa Bay is an intermediate size (258.7mi2) estuary on the southwest coast of Washington State, approximately 30 miles north from the mouth of the Columbia River. Recent studies completed by the National Marine Fisheries Service have shown that significant aggregations of green sturgeon in Willapa Bay occur during the late summer months, and foraging activity for burrowing ghost shrimp (Neotrypaea californiensis) is evidenced by small round feeding pits (30-60 cm diameter) in the …

Two Post-Glacial Sagebrush Steppe Fire Records At The Wildland-Urban Interface, Eastern Cascades, Washington, Dusty Pilkington

All Master's Theses

Recent increases in large fires in the rapidly developing wildland-urban interface (WUI) areas of central Washington, where development intermixes with wildland fuels, contribute to federal firefighting costs exceeding of $1 billion annually. In addition, cheatgrass (Bromus tectorum) invasion and anthropogenic-caused warming shorten fire return intervals while lengthening fire seasons. These climatic, ecological, economic, and social factors combine with fuel accumulation resulting from historic fire suppression to threaten lives and property in the WUI. To plan for safe growth in WUI areas, long-term fire histories are needed to expand understanding of past fire regimes in an understudied ecosystem, sagebrush …

Complex Interactive Effects Of Water Mold, Herbicide, And The Fungus Batrachochytrium Dendrobatidis On Pacific Treefrog Hyliola Regilla Hosts, John M. Romansic, James E. Johnson, R. Steven Wagner, Rebecca H. Hill, Christopher A. Gaulke, Vance T. Vredenburg, Andrew R. Blaustein

Biology Faculty Scholarship

Infectious diseases pose a serious threat to global biodiversity. However, their ecological impacts are not independent of environmental conditions. For example, the pathogenic fungus Batrachochytrium dendrobatidis (Bd), which has contributed to population declines and extinctions in many amphibian species, interacts with several environmental factors to influence its hosts, but potential interactions with other pathogens and environmental contaminants are understudied. We examined the combined effects of Bd, a water mold (Achlya sp.), and the herbicide Roundup® Regular (hereafter, Roundup®) on larval Pacific treefrog Hyliola regilla hosts. We employed a 2 wk, fully factorial laboratory experiment with 3 …

Do Elevated Levels Of Glutathione Decrease The Toxicity Of Plasticizers In Mouse Liver Cells?, Khatoon Albahrani

All Master's Theses

Phthalates are esters of phthalic acid that play a crucial role in the manufacturing industries for enhancing the properties of plastic materials. Di-(2-ethylhexyl) phthalate (DEHP) is one of the most common phthalates used in a variety of products including food packaging and medical equipment. Their use has caused public health concerns because of their toxic effects on reproductive and developmental processes. This study aims to investigate the response of two mouse hepatoma cell lines to the effects of DEHP and its bioactive metabolite mono (2-ethylhexyl) phthalate (MEHP). Cells were exposed to four concentrations (10, 50, 100, and 300 µM) of …

Modeling Sex-Specific Seasonal Habitat Selection For Elk (Cervus Elaphus) In Central Washington, Lewis Meyers

All Master's Theses

Elk (Cervus elaphus) are generalist herbivores, exploiting a variety of environments. I studied habitat selection and sexual segregation of the Colockum elk herd in central Washington. I used a resource selection probability function (RSPF) to evaluate habitat use by males and females during summer and winter seasons. I assisted Washington State Department of Fish and Wildlife (WDFW) in capturing and placing GPS collars on males, and used an existing GPS dataset from females to investigate the extent to which sexual segregation was occurring in the Colockum herd. During summer, males selected steep slopes on north, west and south …

Defining Biodiversity: A Local Assessment Of The Tahuayo River, Peru Using Self-Directed Photography, Rozsika D. Steele

All Master's Theses

The Área de Conservación Regional Comunal Tamshiyacu Tahuayo (ACRCTT), located in Loreto, Peru, protects 420,000 hectares of the Amazon basin. In 2009, the ACRCTT received formal government recognition after three decades of advocacy and conservation work by resident communities. Local resource users who live a subsistence lifestyle possess sophisticated Traditional Ecological Knowledge (TEK) that can be used to identify which constituents of biodiversity are culturally relevant. This information can help resource managers develop an operational definition of biodiversity. Self-directed photography is a research method that allows participants the opportunity to direct data collection and empowers them to visually communicate their …

Urban Stream Burial Increases Watershed-Scale Nitrate Export, Jake J. Beaulieu, Heather E. Golden, Christopher D. Knightes, Paul M. Mayer, Sujay S. Kaushal, Michael J. Pennino, Clay P. Arango, David A. Balz, Colleen M. Elonen, Ken M. Fritz, Brian H. Hill

All Faculty Scholarship for the College of the Sciences

Nitrogen (N) uptake in streams is an important ecosystem service that reduces nutrient loading to downstream ecosystems. Here we synthesize studies that investigated the effects of urban stream burial on N-uptake in two metropolitan areas and use simulation modeling to scale our measurements to the broader watershed scale. We report that nitrate travels on average 18 times farther downstream in buried than in open streams before being removed from the water column, indicating that burial substantially reduces N uptake in streams. Simulation modeling suggests that as burial expands throughout a river network, N uptake rates increase in the remaining open …

Applying Wetland Rating Systems To Assess Functions Of Depressional Wetlands Created By A Mass Wasting Feature, Table Mountain, Washington, Thomas S. Wachholder

All Master's Theses

The formation of wetlands in the Swauk Watershed has been primarily controlled by mass wasting events, which includes landslide activity. Landslide activity has been the primary influential process in shaping the landscape where wetland systems have formed on the surface of landslide deposits. The wetland sites used in this study, near the base of Table Mountain, were chosen because they inhabit the same ancient landslide, have the same underlying geology, and vary in aspect and elevation. The elevational gradient of the sites ranges from 1300 – 1600 m and the individual wetlands differ in terms of north- and south-facing aspects. …

Quantifying Channel Responses To The Removal Of The Glines Canyon Dam In The Middle Reach Of The Elwha River, Washington, Bryon J. Free

All Master's Theses

Four different study sites throughout the middle reach of the Elwha River were monitored before, during, and after the dam removal process over a period of two years from 2012-2014. The complexity of the river geometry was a major factor in the ability of the river to trap and accumulate the new influx of woody debris and sediment from the dam removal, which influenced the response of the river channel. The change that occurred was quantified by using repeat Terrestrial LiDAR (TLS), sediment distribution surveys, and large woody debris mapping techniques. The morphologic changes that occurred during this time were …

Provisioning And Its Effects On The Social Interactions Of Tibetan Macaques (Macaca Thibetana) At Mt. Huangshan, China, Brianna I. Schnepel

All Master's Theses

The dispersal patterns of food resources has a significant effect on the composition of primate groups and social interactions within those groups. Humans often alter the dispersal of food. Non-humans often use affiliative behaviors to elicit tolerance or support from other group members. I investigated whether provisioned food resources alter the social interactions and group dynamics of Macaca thibetana. All-occurrence sampling and scan sampling were used for data recorded by camera traps. Trail-cameras were placed at six locations that contain natural and human food resources and recorded 60-second videos. Social behavior and proximity of the monkeys were recorded. I …

Iron Sources And Dissolved-Particulate Interactions In The Seawater Of The Western Equatorial Pacific, Iron Isotope Perspectives, M. Labatut, F. Lacan, C. Pradoux, J. Chmeleff, A. Radic, J. W. Murray, F. Poitrasson, Anne M. Johansen, F. Thil

All Faculty Scholarship for the College of the Sciences

This work presents iron isotope data in the western equatorial Pacific. Marine aerosols and top core margin sediments display a slightly heavy Fe isotopic composition (δ⁵⁶Fe) of 0.33 ± 0.11‰ (2SD) and 0.14 ± 0.07‰, respectively. Samples reflecting the influence of Papua New Guinea runoff (Sepik River and Rabaul volcano water) are characterized by crustal values. In seawater, Fe is mainly supplied in the particulate form and is found with a δ⁵⁶Fe between −0.49 and 0.34 ± 0.07‰. The particulate Fe seems to be brought mainly by runoff and transported across continental shelves and slopes. …

Effects Of Urban Stream Burial On Organic Matter Dynamics And Reach Scale Nitrate Retention, Jake J. Beaulieu, Paul M. Mayer, Sujay S. Kaushal, Michael J. Pennino, Clay P. Arango, David A. Balz, Timothy J. Canfield, Colleen M. Elonen, Ken M. Fritz, Brian H. Hill, Hodon Ryu, Jorge W. Santo Domingo

All Faculty Scholarship for the College of the Sciences

Nitrogen (N) retention in streams is an important ecosystem service that may be affected by the widespread burial of streams in stormwater pipes in urban watersheds. We predicted that stream burial suppresses the capacity of streams to retain nitrate (NO₃ ⁻) by eliminating primary production, reducing respiration rates and organic matter availability, and increasing specific discharge. We tested these predictions by measuring whole-stream NO₃ ⁻ removal rates using ¹⁵NO₃ ⁻ isotope tracer releases in paired buried and open reaches in three streams in Cincinnati, Ohio (USA) during four seasons. Nitrate uptake lengths were 29 times …

The Elwha River Restoration: Landscape Change, Salmon, And Sense Of Place, Kelseyanne Johnson

All Master's Theses

Removal of the Elwha River dams to restore the ecosystem and salmon fisheries is the largest project of its kind in the history of the United States. Spanning decades and affecting generations, this project has changed and will continue to change the landscape. This research examined the influence of the anticipated return of salmon on 18 key stakeholders' sense of place of the Elwha River. For almost all stakeholders, changes to the Elwha landscape are welcomed as they expect that salmon will restore the ecosystem to its natural state. Narrative data revealed dominant themes in participants' sense of place expressed …

Evaluating The Utility Of Beaver Reintroduction Programs For Enhancing Habitat For Rainbow Trout And Steelhead, Jonathan Rodger Hegna

All Master's Theses

Beaver reintroduction programs are increasingly being viewed as a way to enhance salmonid habitat and production. However, the actual effectiveness of using beavers as a habitat enhancement tool for ESA listed steelhead Oncorhynchus mykiss populations is unknown. We examined the type of habitat, at both the microhabitat and mesohabitat levels, preferred by steelhead in three small streams in the upper Yakima Basin, WA through standard snorkel surveys and habitat measurements. Our results suggest that steelhead in small streams strongly prefer (relative to availability) microhabitats that have deeper water(> 30 cm), slow stream velocities(< 0 .05 mis), and complex cover types. Habitat partitioning among the size-classes (small< 50 mm, medium 50-90 mm, large> 90 mm total length, TL) principally operated …

Experiments To Measure The Effects Of Timber Harvesting Equipment On Surface Lithic Scatters, Douglas J. Baughman

All Master's Theses

The importance of cultural resource preservation cannot be overstated; however local economies are at least as important. Due to conservative archaeological site protection practices in Region 5 of the United States Forest Service, the economy of Northeastern California is being adversely affected. In an attempt to help the Forest Service make more informed management decisions and improve the Northeastern California economy, I undertook experiments on the effects of timber harvesting on lithic scatters on Modoc National Forest. The experiments involved placement of 225 glass tiles (proxy lithics) in each of three plots subject to vehicle traffic and log dragging by …

Spatial And Temporal Patterns In The Microbial Diversity Of A Meromictic Soda Lake In Washington State, Pedro A. Dimitriu, Holly C. Pinkart, Brent M. Peyton, Melanie R. Mormile

All Faculty Scholarship for the College of the Sciences

The microbial community diversity and composition of meromictic Soap Lake were studied using culture-dependent and culture-independent approaches. The water column and sediments were sampled monthly for a year. Denaturing gradient gel electrophoresis of bacterial and archaeal 16S rRNA genes showed an increase in diversity with depth for both groups. Late-summer samples harbored the highest prokaryotic diversity, and the bacteria exhibited less seasonal variability than the archaea. Most-probable-number assays targeting anaerobic microbial guilds were performed to compare summer and fall samples. In both seasons, the anoxic samples appeared to be dominated by lactate-oxidizing sulfate-reducing prokaryotes. High numbers of lactate- and acetate-oxidizing …

Seed Invasion Filters And Forest Fire Severity, Tom R. Cottrell, Paul F. Hessburg, Jonathan A. Betz

Biology Faculty Scholarship

Forest seed dispersal is altered after fire. Using seed traps, we studied impacts of fire severity on timing of seed dispersal, total seed rain, and seed rain richness in patches of high and low severity fire and unburned Douglas-fir (Pseudotsuga menziesii) forests in the Fischer and Tyee fire complexes in the eastern Washington Cascades. Unburned plots had the lowest average seed production. The high severity fire patches in the Fischer Fire Complex had a higher total seed production than low severity fire patches of the same complex. At the Tyee Fire Complex, the total seed production for each …

Land Use Influences The Spatiotemporal Controls On Nitrification And Denitrification In Headwater Streams, Clay P. Arango, J. L. Tank

All Faculty Scholarship for the College of the Sciences

N and C cycles in headwater streams are coupled, and land use can modify these cycles by increasing N availability and removing riparian vegetation. To increase our understanding of how land use modifies the controls on N cycling, we quantified rates of 2 microbial N transformations in a total of 18 agricultural and urban streams (with and without riparian buffers) for 3 y to examine how riparian vegetation and land use influence sediment nitrification and denitrification. Nitrification rates were highest in agricultural streams in late spring. Nitrification was not related to streamwater NH₄⁺ concentrations but was positively related …

Sulfur-Oxidizing Bacteria In Soap Lake (Washington State), A Meromictic, Haloalkaline Lake With An Unprecedented High Sulfide Content, Dimitry Y. Sorokin, Mirjam Foti, Holly C. Pinkart, Gerard Muyzer

All Faculty Scholarship for the College of the Sciences

Culture-dependent and -independent techniques were used to study the diversity of chemolithoautotrophic sulfur-oxidizing bacteria in Soap Lake (Washington State), a meromictic, haloalkaline lake containing an unprecedentedly high sulfide concentration in the anoxic monimolimnion. Both approaches revealed the dominance of bacteria belonging to the genus Thioalkalimicrobium, which are common inhabitants of soda lakes. A dense population of Thioalkalimicrobium (up to 10⁷ cells/ml) was found at the chemocline, which is characterized by a steep oxygen-sulfide gradient. Twelve Thioalkalimicrobium strains exhibiting three different phenotypes were isolated in pure culture from various locations in Soap Lake. The isolates fell into two groups …