Life Sciences Commons^™

Development Of A Rapid Drug Detection Method For Insects Using Paper Spray Ionization Mass Spectrometry (Psi-Ms), Alexandria Plyler

Undergraduate Research and Scholarship Symposium

Insects have the ability to reflect the chemical environment that they have developed in. Thus, necrogenous insects can reflect the chemical environment of cadavers they have fed on. Forensic scientists can potentially utilize insect larvae as an alternate biological matrix to detect drugs in cadavers that are skeletonized, burned, or otherwise badly damaged. Analysis of insects using paper spray ionization mass spectrometry (PSI-MS), an analytical method requiring little to no sample preparation, could prove a rapid, cost-effective, and non-destructive alternative form of toxicological analysis. Traditional tissue analysis via GC and LC-MS often require lengthy sample preparation, use expensive reagents, and …

A Mathematical Model Of Flexible Collective Defense: Crisis Response In Stingless Bees, Maria Gabriela Navas Zuloaga, Kaitlin M. Baudier, Theodore P. Pavlic, Jennifer Fewell, Noam Ben-Asher, Yun Kang

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Juvenile Hormone Mediation In An Insect With Parental Care Behavior, Jessica M. Rodino

UNO Student Research and Creative Activity Fair

Juvenile hormone (JH) is a well-known catalyst for hormonal processes in insects. However, the role of JH in insects that exhibit parental behavior is unknown. We investigated the influence of JH on parental behavior in the burying beetle (Nicrophorus orbicollis). In the first experiment, we manipulated the JH production of females via the administration of varying doses of fluvastatin sodium immediately following oviposition. We found that with increasing fluvastatin dosage, the total mass of offspring and number of offspring decreased while at the same time less of the food source was consumed. These results suggest a link between …

Differential Analysis Of Individual Centruroides Vittatus Venom

Symposium of Student Scholars

Scorpions are well known, venomous arthropods (Class: Arachnida, Order: Scorpiones). Their venom is composed of neurotoxins, proteases and cytotoxic peptides which plays a major role in toxic components such as inflammatory, antimicrobial and hemolytic activity. Venom is key for the survival of scorpions as it is involved in defense against prey and in feeding on predators. Centruroides vittatus (bark scorpion) is commonly found in North America, with habitat ranges from Nebraska to southern Texas. For this study, scorpions were harvested from Laredo, Texas (27˚57’ N, 99˚43’ W), held in captivity for 4 months and fed a consistent diet of Acheta …

Sown And Unsown Floral Resources Both Support Bee Abundance, Pryce Johnson, Kenneth Elgersma, Ai Wen

Summer Undergraduate Research Program (SURP) Symposium

The recent decline in native bee populations across the Midwest has been linked to the drastic decrease of prairie habitats and pollinator floral resources. Since the agricultural revolution, grassland areas have been removed and replaced with row crops to meet the needs of expanding markets, including the production of ethanol. In response to the loss of these (and other) essential pollinators, the Conservation Reserve Program administered by the USDA initiated a pollinator habitat initiative called CP-42. Despite efforts to assess the program's success, there is still much that needs to be learned about restoring prairie habitats for bees. With CP-42, …

Assessing Cp - 42 Habitat Value For Bees Using The Floral Resource Index, Allison Eagan, Gabrielle Brown, Ai Wen

Summer Undergraduate Research Program (SURP) Symposium

Prairies once dominated 85% of Iowa. Sadly, that large portion of native prairie has been drastically reduced to 0.01% because of agricultural expansion. Habitat loss is one of the main contributors to the massive decline in the native bee population and its biodiversity. Native pollinators are essential to the ecosystem and play a large role in the pollination of row crops. The Conservation Reserve Program (CRP), administered by the US Department of Agriculture Farm Service Agency, launched the CP-42 program specifically as a Pollinator Habitat Initiative for forbs and grasses to be planted to increase pollinator habitat. However, no methods …

Local And Landscape Effects On Butterfly Abundance In Cp-42 Plantings, Emma Simpson, Kate Sinnott, Mark Myers

Summer Undergraduate Research Program (SURP) Symposium

The conversion of grassland habitats and intensification of row crop agriculture over the last several decades have driven declines in native pollinator populations throughout the Midwestern USA. These declines in native bee and butterfly abundance have negative ecosystem consequences due to these insects’ important ecological roles as pollinators of vegetable crops and other plant life. The United States Department of Agriculture’s Conservation Reserve Program Pollinator Habitat Initiative (CP-42) aims to conserve native pollinators by increasing pollinator habitat throughout the United States. However, since the program’s inception in 2011, there has been little monitoring of its effectiveness and no documentation of …

Tracing Nutrient Sources To Lipid Production In Insects Using Stable Isotope (Δ13c, Δ2h) Tracers: Implications For Nutritional Physiology Of Migratory Species., Libesha Anparasan, Keith A. Hobson

Western Research Forum

Using stable isotope measurements of insect tissues to determine origin and migratory patterns is well established. However, isotopically determining nutritional origins of lipids, the primary fuel of migration, has not been as thoroughly researched. We explored isotopic links between diet and stored lipids in laboratory raised True armyworm moths (Mythimna unipuncta) using δ¹³C and δ²H measurements. Pupae were randomly separated into four groups (n=20) and fed isotopically distinct nectar, each consisting of a combination of high δ¹³C (C4 sugar), or low δ¹³C (C3 sugar) carbohydrate, with high δ²H (deuterium spiked), …

Age Status Effects On Female Silk Production And Male Courtship Response In Dolomedes Scriptus, Joseph Osborne

Student Scholar Showcase

Female Dolomedes scriptus, the striped fishing spider, serve as model organisms exhibiting pheromone production as a means of chemical signaling. Previous work has shown that male fishing spiders are responsive to female silk, and preferentially court in the presence of silk from mature virgins. In our research, we hypothesized that mature females produced larger quantities of silk than penultimate females in an attempt to elicit greater male mating response. Female spiders of both age statuses were kept for two days in a gridded tank. Sub-sampling of silk lines that crossed a random portion of the grid produced results signifying …

A Chemical In Filter Paper Interferes With Trail Following Behavior In The Termite Reticulitermes Hesperus (Banks), Kathryn Vest

Student Scholar Showcase

Termites follow chemical trails. In previous experiments we dissolved and diluted chemicals in acetone to test if they elicited trail following. Each trail was a straight line across the arena drawn with 10 µl using a glass capillary (Microcaps). We noticed a minor but stable response to trails laid with pure acetone. The response could be caused by contaminated acetone or by a chemical in the filter paper (Watman #5) that lined our arena (petri dish, 10cm diameter). We could confirm that the termites respond to acetone alone. Soxkhlet extraction for 8 hours removed the signal from the filter paper. …

Effects Of Rangeland Management On Milkweed Grazing And Monarch Conservation, Brittany Poynor

UNO Student Research and Creative Activity Fair

Cattle typically avoid consuming milkweed plants that contain high levels of toxic defense compounds, and therefore many people assume cattle avoid all milkweeds. However, common milkweed (Asclepias syriaca) contains only moderate levels of toxic compounds, and observations suggest that cattle regularly consume common milkweed and may even preferentially graze flowers and leaves of this species. These observations directly relate to efforts to add over one billion stems of milkweed to the central USA for monarch butterfly (Danaus plexippus) conservation. If cattle intentionally consume common milkweed and other milkweed species, and if certain management practices can reduce …

A Household Model Of German Cockroach Infestations And Their Effect On Symptoms Of Atopic Asthma, Karen Funderburk

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

The Behavior Response Of Antlion Larvae To Alternating Magnetic Fields, Lindsey Wagner, Caleb L. Adams

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Mosquito Ecology, Arbovirus Dynamics, And Control, Cynthia Lord

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Implementation Of The Tickbot: Results And Implications For Modeling, Alexis White, Armin Bahrani, Amanda Devleeschower, Holly Gaff

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Control Policies And Sensitivity Analysis In A Cutaneous Leishmaniasis Model: A Case Study In Cusco Region, Peru., Rocio M. Caja-Rivera, Ignacio Barradas

Biology and Medicine Through Mathematics Conference

No abstract provided.

The Bees Of A.L. Mangham Jr. Regional Airport, Nacogdoches, Texas, Ryan J. Pingedot, Daniel J. Bennett

Undergraduate Research Conference

The United States is home to about 4,000 species of native bees, and many are critically important due to the pollination services they provide (Buchman & Nabhan 1996). Most of these are inconspicuous, solitary bees that nest in the ground. In recent years, a number of bee species have been shown to be in decline (Burkle 2013). Due to these declines, it is important to study and provide habitat for local bee populations.

As a result of periodic mowing, the surroundings of the A.L. Mangham Jr. Regional Airport in Nacogdoches County, Texas provide a grassy, prairie-like habitat that results in …

Recruitment, Survival, And Parasitism Of Monarchs In Residential Gardens And Conservation Areas, Emily A. Geest

UNO Student Research and Creative Activity Fair

Monarch butterflies (Danaus plexippus) are suffering from declining populations due to habitat loss. To help offset this habitat loss conservationists have encouraged planting milkweed gardens to increase the availability of host plants for larvae. A tachinid fly (Lespesia archippivora) that causes mortality parasitizes monarchs. Understanding the influence these gardens can have on parasitism rates and monarch recruitment is vital to evaluating their effectiveness. I am quantifying egg/larval abundance and demography at conservation areas and residential gardens. Additionally, I am collecting and rearing monarch larvae to compare parasitism rates in both areas. My preliminary results suggest that …

Swimming Mechanisms Of Temperate Forest Ants, Noah D. Gripshover, Evan M. Gora, Stephen P. Yanoviak

Posters-at-the-Capitol

Swimming Mechanisms of Temperate Forest Ants (Camponotus pennsylvanicus and Formica subsericea)

Noah D. Gripshover, Evan M. Gora, and Stephen P. Yanoviak

University of Louisville

Abstract

Environmental challenges shape the evolution of animal behavior and morphology. For wingless terrestrial invertebrates like ants, pools of water on the forest floor are particularly dangerous. Here we show that ants can overcome this obstacle using a modified gait to transverse the water surface. We compared the locomotor morphology and swimming performance of two arboreal ant species that are common in Kentucky (Camponotus pennsylvanicus and Formica subsericea). We defined performance as speed …

The Spatial Distribution And Density Of The Emerald Ash Borer Infestation In Rock Island And Moline, Il, Kendall Smith, Victoria Lason, Tierney Brosius, Michael Reisner

Celebration of Learning

The Emerald Ash Borer (EAB) Agrilus planipennis Fairmaire (Copleoptera: Buprestidae) is a destructive colonizer of ash trees that arrived in the United States in 2002. Since then, EAB has been detected in 22 states and two Canadian provinces, and has proven to be a complicated management issue for many cities including Rock Island. The purpose of this study was to determine the spatial distribution and density of the EAB infestation in Rock Island, Ill and to identify potential correlations between host larval densities and visual symptoms. A continuation of spatial distribution was determined through a tree survey examining specific species …

Ifly: Code Development For An App To Support Automating Entomological Data Collection, Michael P. Cosentino, Trevor Stamper

The Summer Undergraduate Research Fellowship (SURF) Symposium

We are developing a prototype entomological data-collection application called "iFly," which runs on a field-capable iPad device. In this phase, we tackled refining screens and introducing a database manager to streamline operations as info is entered, stored, retrieved and delivered. We used SQLite3 database in Apple's Xcode Integrated Development Environment (IDE). Xcode gives mixed programming results. Apple's iOS environment ensures functional and fairly error-free apps can be built. But the sophisticated Xcode IDE requires specialist developers and valuable project time is spent as new programmers learn key techniques. The iFly prototype was advanced with improved database integration; however, more work …

Dynamics Of Two Pathogens In A Single Tick Population, Alexis White

Biology and Medicine Through Mathematics Conference

No abstract provided.

Hands Of The Future, Inc; Junior Nature Club; Living Schoolyards, Zonda K. Bryant

Purdue P-12 Networking Summit & Poster Session

Programs to connect children to nature

Pollinator Garden, Paige E. Bradley

Engagement & Service-Learning Summit

The goal of the project is to increase awareness about global climate change and the necessity of environmentally sustainable practice, particularly among the younger citizens of the Lafayette area. The Lyn Greece Boys & Girls Club extended a hand and offered us land to install a pollinator garden on their campus and an opportunity to meet with the kids one-on-one to promote sustainability ideas and help them understand the basics of gardening. The project is as local as possible, using native plants, no pesticides or herbicides, Bennett's Greenhouse (a local business), and the resources on campus to reach out to …

Seasonality Of Forensically Relevant Diptera In Northwestern Indiana, Sarah M. Stanley, Trevor Stamper

The Summer Undergraduate Research Fellowship (SURF) Symposium

Minimum postmortem interval (mPMI) estimations are critical to solving cases of equivocal death and the accuracy of these predictions can depend on the understanding of necrophagous fly successional patterns. In order to better understand the successional patterns of necrophagous flies, it is important to know the seasonality of forensically relevant fly species—that is, the baseline pattern of their presence and absence in relation to annually cyclic environmental factors. Since many environmental factors play a role in insect seasonality, it is possible that some of these factors can be summed to create an index that represents seasonality in a simpler form. …

Influence Of Temperature On The Tensile Strength Of Spider Silk (Araneus Gemmoides), Victoria Lekson

Seaver College Research And Scholarly Achievement Symposium

Temperature ranges between day and night in southern California can be as great as 30°C and may influence vital functioning of ectothermic organisms. Arachnids produce silks from a pair of spinnerets in their abdomen and rely on variance in protein composition to make different types of silks. Temperature may influence tensile strength of filaments. We tested the effects of temperature on tensile strength of dragline silk of five specimens of Araneus gemmoides (orb-weaver) which were collected from Malibu Creek State Park in the Santa Monica Mountains of southern California, under five temperature conditions. Each specimen was placed in a 0.0283 …

P-27 Factors Affecting Phonotactic Responses In Male Acheta Domesticus, Erik Thordarson

Honors Scholars & Undergraduate Research Poster Symposium Programs

This research addresses some of the possible factors that affect male Acheta domesticus phonotactic responses to calls of males of the same species. The study examines cricket age and social interaction with females as possible factors affecting this behavior. Through logistic regression analysis, the study attempts to demonstrate a link between these factors and the responsiveness of the male crickets in the study. As the crickets aged, they responded to a wider range of syllable periods, which may imply that they follow a similar pattern of decreasing selectivity in their response with age as their female counterparts. The socialization aspect …

National Park Service Cave Ecology Inventory And Monitoring Framework, Gretchen M. Baker, Steven J. Taylor, Shawn Thomas, Rick Olson, Kathy Lavoie, Marie Denn, Steven Thomas, Hazel Barton, Kurt Helf, Rene Ohms, Joel Despain, Jim Kennedy, David Larson

National Cave and Karst Management Symposium 2013

A team developed the Cave Ecology Inventory and Monitoring Framework for National Park Service (NPS) units. It contains information for NPS cave managers across the United States to determine how to inventory and monitor cave ecology. Due to the wide geographical scope of NPS caves and their many different types, the document does not prescribe exact protocols. Instead, it provides guidance for what types of inventory and monitoring are possible, a framework for deciding how to prioritize inventory and monitoring activities, and references to specific protocols that are already in place at NPS cave parks.

Keywords: cave ecology, cave microbiology, …

Characterization Of Ips Pini Ipsdienol Dehydrogenase (Idol Dh), Heidi Pak, Claus Tittiger, William Welch

Festival of Communities: UG Symposium (Posters)

Ipsdienol is an important pheromone component for pine engraver beetle, Ips pini. Ipsdienol is a ten carbon monoterpenoid secondary alcohol and ipsdienone is the corresponding ketone. We are characterizing the activity of recombinant IDOL DH produced in Sf9 (insect) cells. The enzyme has a high stereospecificity: (-) ipsdienol was found to be a substrate while (+)-ipsdienol was neither a substrate nor inhibitor. Closely related monoterpenoids, such as nerol, geraniol, and citral, were neither substrates nor inhibitors. Smaller compounds, such as 2-propanol, also failed to act as an inhibitor or substrate. This indicates the binding site of this enzyme is highly …

Role Of Ecdysone Signaling In Fat Body Remodeling, Marsha Kristel Bernardo, N. Bond, Allen G. Gibbs

Undergraduate Research Opportunities Program (UROP)

Climate change is fundamentally connected to animal development and survival, and the life history of an organism must be coordinated with predictable seasonal changes of the environment. Climate change affects the life cycle of plants, a major food source for insects. If photoperiod, the primary environmental queue that insects utilize to determine the proper emergence time, and food availability becomes out of sync, many populations of insects and other animals could be threatened. Understanding animal development can provide insight into this issue and could provide clues that may help the scientific community predict how insect populations may respond to climate …