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Articles 31 - 59 of 59
Full-Text Articles in Life Sciences
Chemical Ecology, Population Dynamics And Insecticide Susceptibility Of Lesser Mealworm Alphitobius Diaperinus (Panzer) (Coleoptera: Tenebrionidae), Narinderpal Singh
Chemical Ecology, Population Dynamics And Insecticide Susceptibility Of Lesser Mealworm Alphitobius Diaperinus (Panzer) (Coleoptera: Tenebrionidae), Narinderpal Singh
Graduate Theses and Dissertations
The lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), is a serious, cosmopolitan pest present in poultry production facilities, where it slows down weight gain in chicks, carries disease–causing organisms, and causes damage to poultry insulation. The first study estimated that the lesser mealworm densities in poultry litter applied to pastures were 3.5, 2.2 and 0.4 million larvae, adults and pupae, respectively, per hectare in Savoy, Arkansas. The repercussions of these adult beetles are that they often disperse to and reinfest poultry houses with the potential to vector disease to poultry, and disperse to invade human dwellings resulting in litigation. The …
The Role Of Granulocyte Colony-Stimulating Factor In The Intestinal Immune System And The Response To Probiotics, Andrew J. Martins
The Role Of Granulocyte Colony-Stimulating Factor In The Intestinal Immune System And The Response To Probiotics, Andrew J. Martins
Electronic Thesis and Dissertation Repository
Loss of immunoregulation in the intestine results in inflammation, such as is observed in the chronic, relapsing, inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis. However, our understanding of this process remains incomplete. The cytokine granulocyte-colony stimulating factor (G-CSF) is an important stimulator of granulopoiesis in steady-state and during inflammation, but has also been shown to have anti-inflammatory effects and is a potential therapeutic for IBD. In addition, certain strains of bacteria, termed probiotics, have been postulated to have anti-inflammatory effects in the intestine. The mechanisms by which these strains of bacteria elicit anti-inflammatory effects are not fully understood. …
Assessment Of Pathways For The Introduction And Spread Of Mycobacterium Bovis In The United States, Katie Portacci, Jason Lombard, Lauren Abrahamsen, Eric Bush, Charles Fossler, Robert Harris, Kamina Johnson, Ryan S. Miller, Dianna Mitchell, Randy Pritchard, Steven Sweeney, Todd Weaver
Assessment Of Pathways For The Introduction And Spread Of Mycobacterium Bovis In The United States, Katie Portacci, Jason Lombard, Lauren Abrahamsen, Eric Bush, Charles Fossler, Robert Harris, Kamina Johnson, Ryan S. Miller, Dianna Mitchell, Randy Pritchard, Steven Sweeney, Todd Weaver
Zoonotics and Wildlife Disease: Publications
Bovine tuberculosis (TB) was responsible for more losses among U.S. farm animals in the early 20th century than all other infectious diseases combined. The Cooperative State-Federal Tuberculosis Eradication Program (established in 1917 and administered by APHIS, State animal health agencies, and U.S. livestock producers) has nearly eradicated bovine TB from the nation’s livestock population. However, despite the many accomplishments of the program, bovine TB remains a serious and costly disease of livestock in the United States. In 1992, VS conducted an assessment to identify pathways for the introduction and spread of bovine TB, in order to develop the most effective …
Environmental Problems Of Industrialization And Sustainable Development In Nigeria - A Review, Adejoh Iyaji
Environmental Problems Of Industrialization And Sustainable Development In Nigeria - A Review, Adejoh Iyaji
Confluence Journal Environmental Studies (CJES), Kogi State University, Nigeria
The main objective of this paper is to examine the impact of the quest for industrialization on the environment in Nigeria; others include, the identification of the objectives of sustainable development as well as making recommendations that will lead to the much needed sustainable environment both for the present generation and generations yet unborn. The researcher relied principally on secondary sources of data for this paper which is theoretical in approach. Findings revealed the emergence of a host of environmental problems such as air pollution, deforestation, desertification, solid and hazardous waste problems in the wake of the quest for industrialization. …
The Spatial Epidemiology And Clinical Features Of Reported Cases Of La Crosse Virus Infection In West Virginia From 2003 To 2007, Andrew D. Haddow, Danae Bixler, Agricola Odoi
The Spatial Epidemiology And Clinical Features Of Reported Cases Of La Crosse Virus Infection In West Virginia From 2003 To 2007, Andrew D. Haddow, Danae Bixler, Agricola Odoi
Entomology & Plant Pathology Publications and Other Works
Background
La Crosse virus (LACV) is a major cause of pediatric encephalitis in the United States. Since the mid-1980s, the number of reported cases of LACV infection in West Virginia has continued to rise and the state currently reports the most cases in the United States. The purpose of this study was to investigate and describe the spatial epidemiology and clinical presentation of LACV infection cases reported in West Virginia, as well as to provide a description of the environmental conditions present at the residences of the LACV infection cases.
Methods
Descriptive and spatial analyses were performed on LACV infection …
Unifying The Mathematical Modeling Of In Vivo And In Vitro Microdialysis, Peter M. Bungay, Rachita K. Sumbria, Ulrich Bickel
Unifying The Mathematical Modeling Of In Vivo And In Vitro Microdialysis, Peter M. Bungay, Rachita K. Sumbria, Ulrich Bickel
Pharmacy Faculty Articles and Research
A unifying approach is presented for developing mathematical models of microdialysis that are applicable to both in vitro and in vivo situations. Previous models for cylindrical probes have been limited by accommodating analyte diffusion through the surrounding medium in the radial direction only, i.e., perpendicular to the probe axis, or by incomplete incorporation of diffusion in the axial direction. Both radial and axial diffusion are included in the present work by employing two-dimensional finite element analysis. As in previous models, the nondimensional clearance modulus (Θ) represents the degree to which analyte clearance from the external medium influences diffusion through the …
Spatial Distribution Of Commercial Banks In Ilorin Metropolis, Kwara State, Nigeria, I B. Abdullahi, M A. Ijaiya, A Abdulraheem, R I. Abdulkadir, R O. Ibrahim
Spatial Distribution Of Commercial Banks In Ilorin Metropolis, Kwara State, Nigeria, I B. Abdullahi, M A. Ijaiya, A Abdulraheem, R I. Abdulkadir, R O. Ibrahim
Confluence Journal of Environmental Studies (CJES), Kogi State University, Nigeria
The spatial distribution of banks in any geographic entity determines the level of accessibility to its services by the public. This study examined the pattern of banks distribution in Ilorin metropolis. Field survey was employed in determining the number of available commercial banks and their respective distances between each other. The spatial analysis technique such as the nearest neighbour analysis as used ascertain the degree of clustering, density and the average distance taken to access these services. The study revealed that about 96% of the total number of banks are situated in the Central Business District which exhibited a very …
Risky Behaviour And Hiv Prevalence Among Zambian Men, Nisha Malhotra, Jonathan Young
Risky Behaviour And Hiv Prevalence Among Zambian Men, Nisha Malhotra, Jonathan Young
Nisha Malhotra
The objective of this paper is to identify demographic, social and behavioural risk factors for HIV infection among men in Zambia. In particular, the role of alcohol, condom use, and number of sex partners is highlighted as being significant in the prevalence of HIV. Multivariate Logistic Regressions were used to analyse the latest cross-sectional population-based demographic health survey for Zambia (2007). The survey included socio-economic variables and HIV serostatus for consenting men (N = 4,434). Risk for HIV was positively related to the wealth status. Men who considered themselves to be at high risk for HIV-positive were most likely to …
Recombinant Expression, Protein Purification, And Crystallization Of Thermococcus Thioreducens Str, Ogl-20, Michelle Morris
Recombinant Expression, Protein Purification, And Crystallization Of Thermococcus Thioreducens Str, Ogl-20, Michelle Morris
Summer Community of Scholars Posters (RCEU and HCR Combined Programs)
No abstract provided.
Infection Rate Of Dactylogyrus Parasites On Etheostoma Duryi And Etheostoma Zonale, Kara Million
Infection Rate Of Dactylogyrus Parasites On Etheostoma Duryi And Etheostoma Zonale, Kara Million
Summer Community of Scholars Posters (RCEU and HCR Combined Programs)
No abstract provided.
A Time-Efficient Method For Analyzing Bone Strain With Large Subject Pools, Daniel Leib, Eric Dugan, Henry Wang
A Time-Efficient Method For Analyzing Bone Strain With Large Subject Pools, Daniel Leib, Eric Dugan, Henry Wang
Daniel Leib
No abstract provided.
Adaptive Responses To Flooding Incidents In Ilorin, Kwara State, Nigeria, Habiba I. Jimoh, Kayode A. Iroye
Adaptive Responses To Flooding Incidents In Ilorin, Kwara State, Nigeria, Habiba I. Jimoh, Kayode A. Iroye
Confluence Journal Environmental Studies (CJES), Kogi State University, Nigeria
Incidents of floods which are mainly caused by changes in landuse is fast becoming a city life experience in Ilorin as in most urban centres in Nigeria causing untold hardships and sometimes loss of lives. This extreme hydro-meteorological event is also being exacerbated by climate change which thus calls for adaptive response by residents towards reducing its risks, hence this study. Data used were generated from direct field measurements and questionnaire administration. Descriptive statistics and cross tabulations were used in analyzing the data. Results obtained indicate that most respondents use a wide range of non-structural adaptive response to flood. The …
An Assessment Of Socio-Economic Impact Of Waste Scavenging As A Means Of Poverty Alleviation In Gwagwalada, Abuja., John Yakubu Magaji, Samuel Panse Dakyes
An Assessment Of Socio-Economic Impact Of Waste Scavenging As A Means Of Poverty Alleviation In Gwagwalada, Abuja., John Yakubu Magaji, Samuel Panse Dakyes
Confluence Journal Environmental Studies (CJES), Kogi State University, Nigeria
Waste scavengers are usually perceived as being among the poor, and scavenging is considered a marginal activity. They tend to have low incomes, but can obtained decent earning when they are not exploited by middlemen. This study was conducted in Gwagwalada town with the aim of assessing the socio-economic impact of scavenging on the people. A structured questionnaire was constructed to capture the demographic characteristics of the scavengers, their experiences, types of items scavenged, the economic gains and the challenges being faced. The target pollution is waste scavengers and a random sampling technique was adopted in selecting the respondents for …
Does A Diet That Consists Of High Fiber Prevent The Recurrence Of Colorectal Adenomas In Patients Who Have Previously Had At Least One Adenoma Detected Via Colonoscopy?, Conor Luskin
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: To determine whether ingesting a diet high in fiber will decrease the incidence of colorectal adenoma recurrence in a person who has previously had colorectal adenomas.
Lack Of Processing Of The Expressed Orf1 Gene Product Of Hepatitis E Virus, Suganthi Suppiah, Yumei Zhou, Teryl K. Frey
Lack Of Processing Of The Expressed Orf1 Gene Product Of Hepatitis E Virus, Suganthi Suppiah, Yumei Zhou, Teryl K. Frey
Biology Faculty Publications
Background: Proteolytic processing is a common mechanism among plus strand RNA viruses and the replicases of all plus strand RNA viruses of animals thus far characterized undergo such processing. The replicase proteins of hepatitis E virus (HEV) are encoded by ORF1. A previous report published by our group [1] provided data that processing potentially occurred when ORF1 (Burma strain; genotype 1) was expressed using a vaccinia virus-based expression system.
Findings: To further test for processing and to rule out artifacts associated with the expression system, ORF1 was re-expressed using a plasmid-based expression vector with the result that the previous processing …
Horse Alert Wa, Department Of Agriculture And Food, Western Australia
Horse Alert Wa, Department Of Agriculture And Food, Western Australia
All other publications
Increasing awareness of emergency horse diseases, their management in Western Australia and practical strategies to minimise the risk of spreading disease. Horse Alert WA has been developed to help participants in the racing, sporting and recreational sectors of the horse industry in Western Australia to prepare for, recognise and respond effectively to an emergency disease that affects horses, such as the equine influenza outbreak in Australia in 2007. This manual provides biosecurity information to minimise the risks of a horse disease establishing and spreading in Western Australia, and guidance on the actions to take if an emergency disease occurs. Some …
Going Viral, Judy Diamond
Going Viral, Judy Diamond
World of Viruses
Science education is developing new and innovative means of communication that compete directly with global media to reach the ‘YouTube Generation’. The truly interdisciplinary team of the SEPA-funded World of Viruses project is discovering how best to spread the word.
KEY COLLABORATORS
Judy Diamond, Professor, University of Nebraska State Museum
Charles Wood, Director, Nebraska Center for Virology
Moira Rankin, President, Soundprint Media Center, Inc
Carl Zimmer, Science writer and author of WoV book of essays, A Planet of Viruses
David Uttal, Cognitive science professor, Northwestern University
Benjamin Jee, Cognitive scientist, College of Holy Cross
Tom Floyd, Comic illustrator, Nebraska Educational …
Clinical Use Of Probiotics: A Survey Of Physicians’ Beliefs And Practice Patterns, Anastasia Ensminger
Clinical Use Of Probiotics: A Survey Of Physicians’ Beliefs And Practice Patterns, Anastasia Ensminger
Master's Theses and Doctoral Dissertations
Background: Probiotics have the potential to be used as a preventive agent or adjuvant therapy for various medical conditions, and recent research is beginning to illuminate some of the associated benefits. Some clinicians currently prescribe probiotics in practice. Understanding physicians’ beliefs and practice patterns regarding the use of probiotics will help identify current practices, barriers preventing their acceptance, and the sources of information that impact clinical practice.
Objective: To identify and describe physicians’ beliefs and practice patterns regarding the use of probiotics.
Methods: A cross-sectional online questionnaire was administered to 130 physicians employed by or affiliated with Danville …
Estimating The Reproductive Numbers For The 2008-2009 Cholera Outbreaks In Zimbabwe, Zindoga Mukandavire, Shu Liao, Jin Wang, Holly Gaff, David L. Smith, J. Glenn Morris Jr.
Estimating The Reproductive Numbers For The 2008-2009 Cholera Outbreaks In Zimbabwe, Zindoga Mukandavire, Shu Liao, Jin Wang, Holly Gaff, David L. Smith, J. Glenn Morris Jr.
Biological Sciences Faculty Publications
Cholera remains an important global cause of morbidity and mortality, capable of causing periodic epidemic disease. Beginning in August 2008, a major cholera epidemic occurred in Zimbabwe, with 98,585 reported cases and 4,287 deaths. The dynamics of such outbreaks, particularly in nonestuarine regions, are not well understood. We explored the utility of mathematical models in understanding transmission dynamics of cholera and in assessing the magnitude of interventions necessary to control epidemic disease. Weekly data on reported cholera cases were obtained from the Zimbabwe Ministry of Health and Child Welfare (MoHCW) for the period from November 13, 2008 to July 31, …
2011-2012 Unlv Mcnair Journal, Cyndy Anang, Sajar Camara, Pamela Cornejo, Carla Antonieta Farcello, Ilse Anahi Garcia, Natiera Magnuson, William L. Mccurdy, Lorena Munoz, Maxym V. Myroshnychenko, Ricardo Rios, Theodore Waldeck, Barbara Wallen, Ana Zuniga, Brenda M. Aguilar, Tiffany Alexandra Alvarez, Daniel N. Erosa, Paige C. Espinosa, Carla Antonieta Farcello, Julienne Jochel Paraiso, Nathaniel Derek Phillipps, Carmen Vallin, Jacent N. Wamala, Ernesto Zamora-Ramos
2011-2012 Unlv Mcnair Journal, Cyndy Anang, Sajar Camara, Pamela Cornejo, Carla Antonieta Farcello, Ilse Anahi Garcia, Natiera Magnuson, William L. Mccurdy, Lorena Munoz, Maxym V. Myroshnychenko, Ricardo Rios, Theodore Waldeck, Barbara Wallen, Ana Zuniga, Brenda M. Aguilar, Tiffany Alexandra Alvarez, Daniel N. Erosa, Paige C. Espinosa, Carla Antonieta Farcello, Julienne Jochel Paraiso, Nathaniel Derek Phillipps, Carmen Vallin, Jacent N. Wamala, Ernesto Zamora-Ramos
McNair Journal
Journal articles based on research conducted by undergraduate students in the McNair Scholars Program
Table of Contents
Biography of Dr. Ronald E. McNair
Statements:
Dr. Neal J. Smatresk, UNLV President
Dr. Juanita P. Fain, Vice President of Student Affairs
Dr. William W. Sullivan, Associate Vice President for Retention and Outreach
Mr. Keith Rogers, Deputy Executive Director of the Center for Academic Enrichment and Outreach
McNair Scholars Institute Staff
The Impact Of Infectious Disease On Chronic Disease: A Review Of Contemporary Findings, Wendy Bjerke
The Impact Of Infectious Disease On Chronic Disease: A Review Of Contemporary Findings, Wendy Bjerke
All PTHMS Faculty Publications
Infectious diseases are among the top causes of death in adults and children. Chronic diseases such as cardiovascular disease and cancer are among the top causes of death in adults. Associations between bacterial and viral infectious agents and subsequent development of chronic disease have been made in the past but are currently being reexamined with more rigor and specificity. This review examines infectious disease agent causes and associations with cancer, cardiovascular disease, metabolic disease, and neurodegenerative disease. These associations will impact future research, surveillance, treatment, and prevention of both infectious and chronic diseases.
Tca Cycle Inactivation In Staphylococcus Aureus Alters Nitric Oxide Production In Raw 264.7 Cells, Chandirasegaran Massilamany, Arunakumar Gangaplara, Donald J. Gardner, James M. Musser, David J. Steffen, Greg A. Somerville, Jay Reddy
Tca Cycle Inactivation In Staphylococcus Aureus Alters Nitric Oxide Production In Raw 264.7 Cells, Chandirasegaran Massilamany, Arunakumar Gangaplara, Donald J. Gardner, James M. Musser, David J. Steffen, Greg A. Somerville, Jay Reddy
Jay Reddy Publications
Inactivation of the Staphylococcus aureus tricarboxylic acid (TCA) cycle delays the resolution of cutaneous ulcers in a mouse soft tissue infection model. In this study, it was observed that cutaneous lesions in mice infected with wild-type or isogenic aconitase mutant S. aureus strains contained comparable inflammatory infiltrates, suggesting the delayed resolution was independent of the recruitment of immune cells. These observations led us to hypothesize that staphylococcal metabolism can modulate the host immune response. Using an in vitro model system involving RAW 264.7 cells, the authors observed that cells cultured with S. aureus aconitase mutant strains produced significantly lower amounts …
Detection Of Cardiac Myosin Heavy Chain-Α-Specific Cd4 Cells By Using Mhc Class Ii/IaK Tetramers In A/J Mice, Chandirasegara Massilamany, Arunakumar Gangaplara, Nora M. Chapman, Noel Rose, Jay Reddy
Detection Of Cardiac Myosin Heavy Chain-Α-Specific Cd4 Cells By Using Mhc Class Ii/IaK Tetramers In A/J Mice, Chandirasegara Massilamany, Arunakumar Gangaplara, Nora M. Chapman, Noel Rose, Jay Reddy
Jay Reddy Publications
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with cardiac myosin heavy chain (Myhc)-α 334–352, whereas B10.A mice carrying a similar allele IAk are relatively resistant, suggesting that the generation of Myhc-α-reactive T cell repertoires is influenced by genetic background. To enumerate the precursor frequencies of Myhc-α-specific CD4 T cells, we sought to create IAk tetramers for Myhc-α 334–352. Tetramers were created using approaches that involve covalent tethering of individual peptide sequences or exogenous loading of peptides into empty IAk molecules by peptide-exchange reaction. Using ribonuclease 43– 56 tetramers as controls, we demonstrated that …
Overuse Of Antibiotics In Children For Upper Respiratory Infections (Uris): A Dilemma, Malika Taufiq, Rukhsana Wamiq Zuberi
Overuse Of Antibiotics In Children For Upper Respiratory Infections (Uris): A Dilemma, Malika Taufiq, Rukhsana Wamiq Zuberi
Department for Educational Development
No abstract provided.
Genetic Diversity Of The Pathogen Streptococcus Parauberis Isolated From Bovine And Piscine Hosts, Stephen Douglas Cole
Genetic Diversity Of The Pathogen Streptococcus Parauberis Isolated From Bovine And Piscine Hosts, Stephen Douglas Cole
Dissertations, Theses, and Masters Projects
No abstract provided.
Calf Pre-Weaning Traits And Immunoglobulin Response To Bovine Viral Diarrhea Virus Vaccination, Whitney Downum, A. Hayden Brown Jr., Jermey G. Powell, E. Beth Kegley, Zelpha B. Johnson, Doug B. Galloway, James A. Hornsby, Billy R. Lindsey
Calf Pre-Weaning Traits And Immunoglobulin Response To Bovine Viral Diarrhea Virus Vaccination, Whitney Downum, A. Hayden Brown Jr., Jermey G. Powell, E. Beth Kegley, Zelpha B. Johnson, Doug B. Galloway, James A. Hornsby, Billy R. Lindsey
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Calfhood vaccination for bovine viral diarrhea virus (BVDV) is a relatively new concept, and protocols are evolving. Our objective was to determine effects of BVDV type I vaccination protocol, calf behavior (chute score, and chute exit velocity), and gender on calf gain and immunoglobulin (Ig) response. Crossbred calves (n = 64) were randomly allotted to one of two vaccination protocols. In protocol 1, calves were vaccinated at 60 d of age (d 0) and at weaning (d 147). Calves assigned to protocol 2 were vaccinated against BVDV type I at 21 d prior to (d 126) and at weaning (d …
Preventing Mother-To-Child Transmission Of Human Immunodeficiency Virus-1 (Hiv-1): Effects Of Intrapartum And Neonatal Single-Dose Nevirapine Prophylaxis And Subsequent Hiv-1 Drug Resistance At Antiretroviral Treatment Initiation, Amanda L. Harmon
CMC Senior Theses
The prevention of mother-to-child transmission is one of the most powerful tools in human immunodeficiency virus type 1 (HIV-1) prevention and has huge potential to improve both maternal and child health. In the absence of any preventative measures, infants born to and breastfed by their HIV-positive mothers have roughly a one-in-three chance of acquiring the infection themselves. HIV can be passed on from mother-to-child during pregnancy, during labor and delivery, and even after during breastfeeding.
Intrapartum and neonatal single-dose nevirapine (sd-NVP) is the foundation of preventing mother-to-child transmission in lower resource settings where it has been used alone or as …
Comparative Study Of Long-And Short-Pulsed Electric Fields For Treating Melanoma In An In Vivo Mouse Model, Xinhua Chen, Xinmei Chen, Karl H. Schoenbach, Shusen Zheng, R. James Swanson
Comparative Study Of Long-And Short-Pulsed Electric Fields For Treating Melanoma In An In Vivo Mouse Model, Xinhua Chen, Xinmei Chen, Karl H. Schoenbach, Shusen Zheng, R. James Swanson
Bioelectrics Publications
A mouse melanoma model was set up with green fluorescent protein (GFP) expression in vivo. With the same energy, long- (1 ms) and short- (300 ns) pulsed electric fields were delivered to two melanomas injected into the same mouse. The tumor growth and green fluorescence were followed up to compare the different treatment efficacy of long and short pulses. After two days post treatment, short pulse-treated tumors showed a significantly lower tumor volume compared with long pulse-treated tumors (n=8, p
Ixodes Affinis (Acari: Ixodidae) In Southeastern Virginia And Implications For The Spread Of Borrelia Burgdorferi, The Agent Of Lyme Disease, Robyn M. Nadolny, Chelsea L. Wright, Wayne L. Hynes, Daniel E. Sonenshine, Holly Gaff
Ixodes Affinis (Acari: Ixodidae) In Southeastern Virginia And Implications For The Spread Of Borrelia Burgdorferi, The Agent Of Lyme Disease, Robyn M. Nadolny, Chelsea L. Wright, Wayne L. Hynes, Daniel E. Sonenshine, Holly Gaff
Biological Sciences Faculty Publications
Ixodes affinis Neumann is a hard-bodied (ixodid) tick known to be a competent vector for Borrelia burgdorferi, the agent of Lyme disease, and agents of other human diseases (Keirans et al. 1999). Ixodes affinis has been reported in Florida, Georgia, and South Carolina and throughout coastal North Carolina (Clark et al. 1998, Harrison et al. 2010). Harrison et al. (2010) indicated that I. affinis was established throughout the coastal plain of North Carolina up to the Virginia border and suggested that I. affinis might occur in Virginia.