Life Sciences Commons^™

Simian Virus 40 Host Range/Helper Function Mutations Cause Multiple Defects In Viral Late Gene Expression., Terryl Stacy, Michele Chamberlain, Charles N. Cole

Dartmouth Scholarship

Simian virus 40 (SV40) deletion mutants dlA2459 and dlA2475 express T antigens that lack the normal carboxy terminus. These mutants are called host range/helper function (hr/hf) mutants because they form plaques at 37 degrees C on BSC-1 and Vero monkey kidney cell lines but not on CV-1p monkey kidney cells. Wild-type SV40 can provide a helper function to permit growth of human adenoviruses in monkey kidney cells; the hr/hf mutants cannot. Progeny yields of hr/hf mutants are also cold sensitive in all cell lines tested. Patterns of viral macromolecular synthesis in three cell lines (Vero, BSC-1, and CV-1) at three …

In Vitro Evaluation Of Cancer Patient Immune Responses Following Infusion Of Radiolabeled Murine Monoclonal Antibody, Kelly A. King

Loma Linda University Electronic Theses, Dissertations & Projects

Several monoclonal antibody (MAb) types derived from mice have been developed specifically for the use of targeting human tumors. The specificity of these murine MAb’s for their respective tumor antigens is very high making them potentially good immunotherapeutic agents for cancer treatment and diagnosis. A limiting factor in using these murine MAb’s in patients is the development of human anti-mouse antibody (HAMA). The presence of HAMA may reduce the effectiveness of MAb for tumor targeting. If patient sensitization could be quickly detected following the initial infusion of MAb, then future infusion could be altered or terminated to prevent further sensitization. …

Autonomic Nervous System And Cholesterol Transport, Yuan-Line Hung

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of autonomic nervous system on cholesterol transport is of clinical interest because the relationship between lipoprotein metabolism and atherogenesis is expressed in coronary artery disease.

Generally speaking, beta blockers increase serum VLDL level but decrease both HDL and HDL₂ cholesterol levels without affecting the LDL in humans. We are unaware of reports concerning the effects of metoprolol on lipoprotein metabolism nor comparison of the effects of metoprolol and propranolol in the unhandled rat model. In part I we employed the unhandled rats to compared the effects of these 2 drugs administered with the drinking water in order …

Enhancement Of Intratumor Antibody Distribution With A Bifunctional Antibody Delivery System And With Hyperthermia, Karen Lynn King

Loma Linda University Electronic Theses, Dissertations & Projects

The intratumor distribution of a new monoclonal antibody-hapten-based delivery system was examined. From two marine antibodies, Hybritech, Inc. has developed a bifunctional antibody (BPA) with specificities against CEA (ZCE-025 or GEM 231) and against one of two derivatives of [¹¹¹In]-benzyl-EDTA (CHA 255), EOTUBE or BLEDTA IV, to form ZCE/CHA or CEM/CHA. BFA accumulates in CEA-expressing tissue and clears from non-antigenic tissues prior to administration of an [¹¹¹In]-hapten.

Using five groups of nude mice bearing human colon tumor xenografts (T380), we evaluated different carriers of [¹¹¹In]-EOTUBE to prelocalized ZCE/CHA. We administered all antibodies and haptens …

Breeding Phomopsis-Resistant Lupins, John Sylvester Gladstones

Journal of the Department of Agriculture, Western Australia, Series 4

The release of Gungurru and Yorrel lupins marks the coming of age of the narrow-leafed lupin as a crop plantt. These are the first cultivars of the species th have substantial resistance to Phomopsis stem blight, which will make the stubbles much safer for grazing stock.

This article describes the new lupins, their background, and how they were bred. It also acknowledges the contributions of colleagues who helped in important ways.

Economic Impact Of Growing Phomopsis-Resistant Lupins, J M. Warren, Jeremy Allen, Wallace Cowling

Journal of the Department of Agriculture, Western Australia, Series 4

The planting of Phomopsis-resistant lupins will increase net farm income. They will also alter the traditional blend of pastures and cropping, depending on grain and wool prices. In mixed farming areas of Western Australia's southern wheatbelt, they will allow more land to be sown to lupins rather than cereals on farms in which the lack of sheep feed over summer severely restricts wool production.

The benefits of Phompsis-resistant lupins arise from a reduction in sheep deaths due to lupinosis, a longer safe grazing period (free from lupinosis) on lupin stubbles, a decrease in the need for supplementary sheep feed over …

Leaf Diseases Of Wheat And Time Of Sowing, J M. Wilson

Journal of the Department of Agriculture, Western Australia, Series 4

Leaf diseases of wheat can be avoided by delaying the planting of a crop, but this often leads to reduced yield because the benefits of early sowing usually outweigh anylosses caused by leaf disease.

However, it is only in exceptional circumstances that farmers need to consider leaf disease when making decisions about planting date, as this article shows. Most examples are taken from department of Agriculture trials in its northern advisory region, but the principals apply to other agricultural areas.

Farmers' Estimations Of Sheep Weights To Calculate Drench Dose, R B. Besier, Diane Hopkins

Journal of the Department of Agriculture, Western Australia, Series 4

Undersosing with drenches is a mojor cause of anthelmintic (drench) resistance in worm parasites of sheep.

When farmers use a drench at less than the recommended dose rate, some worms may survive, and their ability to resist treatment with the chemical involved is passed to their offspring. Over a period of time, matings between worms with different degrees of resistance can produce individuals able to resist the drench at the recommended dose rate or even higher.

Farmers must use adequate dose rates so that worms do not have a chance to survive treatment.

Case Report Of Human Infection With Capillaria Philippinensis, Ching-Yu Chen, Wei-Chuan Hsieh, Tsang-Lie Chen

Harold W. Manter Laboratory: Library Materials

In July 1988, a case of human infection with Capillaria philippinensis, so-called the intestinal capillariasis, was discovered at the National Taiwan University Hospital (NTUH). This is the first case reported in Taiwan.

Capillaria philippinesis is a parasite of fish-eating birds In nature it has a fish-bird life cycle. When human populations disrupt the natural cycle by eating raw fish infected with the larvae of C. philippinensis, they may acquire the disease.

Evaluation Of The Crack Approach For The Control Of Drench Resistance In Sheep Worms, J R. Edwards, Helen Chapman, Jon Dunsmore

Journal of the Department of Agriculture, Western Australia, Series 4

The Department of Agriculture launched the CRACK approach to worm control in September 1985 in response to finding that anthelmintic (drench) resistant worms were present on 68 per cent of a random selection of Western Australian sheep farms.

The high cost of internal parasites in terms of lost production and drench costs, together with the high prevalence of resistant worms of farms, confirmed that changes were needed to existing parasite control measures in sheep.l