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Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Assembly Of Α4Β2 Nicotinic Acetylcholine Receptors Assessed With Functional Fluorescently Labeled Subunits: Effects Of Localization, Trafficking, And Nicotine-Induced Upregulation In Clonal Mammalian Cells And In Cultured Midbrain Neurons, Raad Nashmi, Mary E. Dickinson, Sheri Mckinney, Mark Jareb, Cesar Labarca, Scott E. Fraser E. Fraser, Henry A. Lester
Assembly Of Α4Β2 Nicotinic Acetylcholine Receptors Assessed With Functional Fluorescently Labeled Subunits: Effects Of Localization, Trafficking, And Nicotine-Induced Upregulation In Clonal Mammalian Cells And In Cultured Midbrain Neurons, Raad Nashmi, Mary E. Dickinson, Sheri Mckinney, Mark Jareb, Cesar Labarca, Scott E. Fraser E. Fraser, Henry A. Lester
Biology Faculty Publications
Fura-2 recording of Ca2+ influx was used to show that incubation in 1 μM nicotine (2–6d) upregulates several pharmacological components of acetylcholine (ACh) responses in ventral midbrain cultures, including a MLA-resistant, DHβE-sensitive component that presumably corresponds to α4β2 receptors. To study changes in α4β2 receptor levels and assembly during this upregulation, we incorporated yellow and cyan fluorescent proteins (YFPs and CFPs) into the α4 or β2 M3–M4 intracellular loops, and these subunits were coexpressed in human embryonic kidney (HEK) 293T cells and cultured ventral midbrain neurons. The fluorescent …
Epilepsy-Associated Dysfunction In The Voltage-Gated Neuronal Sodium Channel Scn1a, Christoph Lossin, T. Rhodes, R. Desai, C. Vanoye, S. Caniciu, O. Devinsky, A. George
Epilepsy-Associated Dysfunction In The Voltage-Gated Neuronal Sodium Channel Scn1a, Christoph Lossin, T. Rhodes, R. Desai, C. Vanoye, S. Caniciu, O. Devinsky, A. George
Christoph Lossin, Ph.D.
Mutations in SCN1A, the gene encoding the brain voltage-gated sodium channel subunit (Nav1.1) are associated with at least two forms of epilepsy, generalized epilepsy with febrile seizures plus (GEFS+) and severe myoclonic epilepsy of infancy (SMEI). We examined the functional properties of four GEFS+ alleles and one SMEI allele using whole-cell patch-clamp analysis of heterologously expressed recombinant human SCN1A. One previously reported GEFS+ mutation (I1656M) and an additional novel allele (R1657C), both affecting residues in a voltage-sensing S4 segment, exhibited a similar depolarizing shift in the voltage dependence of activation. Additionally, R1657C showed a 50% reduction in current density and …
Is Hemoglobin Gelation An Adaptation To The Cold In Boreal Fishes?, Ione Hunt Von Herbing
Is Hemoglobin Gelation An Adaptation To The Cold In Boreal Fishes?, Ione Hunt Von Herbing
University of Maine Office of Research Administration: Grant Reports
Recent studies of fish red blood cells found that a regular paracrystalline array of hemoglobin (Hb) tetamers formed under low oxygen conditions in 2 species of boreal fishes, Atlantic cod (Gadus morhua) and toadfish, (Opsanus tau). This phenomenon is termed hemoglobin gelation and its physiological characteristics and importance to survival of boreal fishes is unknown. The study outlined in this proposal will obtain preliminary data on the frequency and physiological nature of the phenomenon of hemoglobin (Hb) gelation in red blood cells of fishes that inhabit cold-water temperate and Arctic environments. The present study will test the hypothesis that hemoglobin …
Exposure Of Nervous System Cells To Polychlorinated Biphenyls (Pcbs) Results In Significant Alterations In Levels Of Expression Of Neurotrophic Factors, Charles F. Ide, Jay C. Means, Trisha M. Basford, John Spitsbergen
Exposure Of Nervous System Cells To Polychlorinated Biphenyls (Pcbs) Results In Significant Alterations In Levels Of Expression Of Neurotrophic Factors, Charles F. Ide, Jay C. Means, Trisha M. Basford, John Spitsbergen
Biological Sciences Faculty and Graduate Student Research
Toxic insult by PCBs results in learning and memory deficits in humans. Alterations in expression of neurotrophic factors and/or their receptors have been linked to changes in cognition. How PCBs affect cognition is not known. We propose that PCBs affect cognition by altering neurotrophic factor expression or effects. We exposed cultured C6 glial cells in medium containing PCB (Aroclor 1254 (10ppm)). Control cells were treated with DMSO or regular medium. Cells were incubated at 37o C for up to 72 hours. Medium samples were taken at 6hr, 24hr, 48hr, and 72hr intervals. Enzyme-linked immunosorbent assays (ELISA) were used to determine …