Life Sciences Commons^™

Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We …

Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen is an essential nutrient required for growth and development in plants. Insufficient nitrogen availability can reduce vegetative growth and grain yield. However, nitrogen is a costly input for farmers, is energy intensive to manufacture, and runoff of excess nitrogen fertilizer impacts water quality. Compared to its close relative, maize, sorghum has much greater resilience to nitrogen and water deficit, and heat stress, allowing sorghum to be grown with fewer inputs and on marginal land. Variation in total biomass accumulation and grain yield between sorghum accessions, as well as between nitrogen conditions, can be largely explained by differences in vegetative …

Dynamics Of Co2 Consumption, And Biomass And Lipid Carbon Production During Photobioreactor Cultivation Of The Diatom Cyclotella, Altan Özkan

Turkish Journal of Botany

Understanding of CO₂ delivery and consumption dynamics in algal photobioreactors are critical to unravel microalgae?s full potential for bioproduct generation and carbon capture from flue gas streams. This study aims to expand our current understanding by cultivating the diatom Cyclotella under controlled process conditions of a bubble column photobioreactor and analyzing CO₂ consumption dynamics in real time using results from an online CO₂ sensor connected to the reactor exhaust. Two sets of experiments were conducted: they served to contrast the influence of silicon and nitrate (Si&N colimitation) and Si limitation, and the light availability, respectively. CO2 consumption …

Quantifying The Carbon Stored And Sequestered By The Trees On Pomona College’S Campus, Paola A. Giron-Carson

Scripps Senior Theses

We are experiencing a climate crisis that must be confronted with strategic mitigation. Pomona College contributes to the climate crisis through its emissions for which there is a baseline record. However there is no baseline record of the climate mitigation currently performed by the trees on Pomona’s campus through carbon storage. This study seeks to determine a current baseline quantity of carbon stored and sequestrated by Pomona’s trees as well as possible courses of climate mitigation for Pomona College to take. Initial information gathering was conducted through interviews with several stakeholders. This study was conducted using data collected prior to …

Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm

MSU Graduate Theses

Giant cane (Arundinaria gigantea) is a native species to 22 states in the U.S. The species and its ecosystem are considered critically endangered, and the species has been reduced to 2% of its original extent. The species has a long cultural and conservation history. Large canebrakes were commonly found in Missouri in bottomland forests, stream and riverbanks, and margins of lakes. My research goals were to: 1) examine methods for propagation success from field to greenhouse to field; 2) examine the physiology of cane at one of the few current canebrakes, for greenhouse propagated plants, and field planted …

Ratio-Based Vegetation Indices For Biomass Estimation Depending On Grassland Characteristics, Ahmet Karakoç, Murat Karabulut

Turkish Journal of Botany

Aboveground biomass (AGB) is one of the key indicators of aboveground net primary productivity (ANPP). The aim of this study is to demonstrate the potential of hyperspectral remote sensing techniques to predict AGB in grasslands. In order to reach this goal, biomass properties with different ecological features and altitudes of 550 m, 1200 m, and 1400 m above sea level were investigated. Twenty-one biomass samples and hyperspectral measurements were collected from each region and a total of 63 samples were analyzed. Linear and nonlinear regression models were generated to analyze the relationships between biomass and hyperspectral vegetation indices (VIs). The …

Elucidating Sorghum Biomass, Nitrogen And Chlorophyll Contents With Spectral And Morphological Traits Derived From Unmanned Aircraft System, Jiating Li, Yeyin Shi, Arun-Narenthiran Veeranampalayam-Sivakumar, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Unmanned aircraft systems (UAS) provide an efficient way to phenotype cropmorphology with spectral traits such as plant height, canopy cover and various vegetation indices (VIs) providing information to elucidate genotypic responses to the environment. In this study, we investigated the potential use of UAS-derived traits to elucidate biomass, nitrogen and chlorophyll content in sorghum under nitrogen stress treatments. A nitrogen stress trial located in Nebraska, USA, contained 24 different sorghum lines, 2 nitrogen treatments and 8 replications, for a total of 384 plots. Morphological and spectral traits including plant height, canopy cover and various VIs were derived from UAS flights …

Biomass Production Of Herbaceous Energy Crops In The United States: Field Trial Results And Yield Potential Maps From The Multiyear Regional Feedstock Partnership, Dokyoung Lee, Ezra Aberle, Eric K. Anderson, William Anderson, Brian S. Baldwin, David D. Baltensperger, Michael Barrett, Jurg Blumenthal, Stacy Bonos, Joe Bouton, David I. Bransby, Charlie Brummer, Pane S. Burks, Chengci Chen, Christopher Daly, Jose Egenolf, Rodney L. Farris, John H. Fike, Roch E. Gaussoin, John R. Gill, Kenneth Gravois, Michael D. Halbleib, Anna Hale, Wayne Hanna, Keith Harmoney, Emily A. Heaton, Ron W. Heiniger, Lindsey Hoffman, Chang O. Hong, Gopal Kakani, Robert Kallenbach, Bisoondat Macoon, James C. Medley, Ali Missaoui, Robert B. Mitchell, Ken J. Moore, Jesse I. Morrison, Gary N. Odvody, Jonathan D. Richwine, Richard Ogoshi, Jimmy Ray Parrish, Lauren Quinn, Ed Richard, William L. Rooney, J. Brett Rushing, Ronnie Schnell, Matt Sousek, Scott A. Staggenborg, Thomas Tew, Goro Uehara, Donald R. Viands, Thomas Voigt, David Williams, Linda Williams, Lloyd Ted Wilson, Andrew Wycislo, Yubin Yang, Vance Owens

Department of Agronomy and Horticulture: Faculty Publications

Current knowledge of yield potential and best agronomic management practices for perennial bioenergy grasses is primarily derived from small-scale and short-term studies, yet these studies inform policy at the national scale. In an effort to learn more about how bioenergy grasses perform across multiple locations and years, the U.S. Department of Energy (US DOE)/Sun Grant Initiative Regional Feedstock Partnership was initiated in 2008. The objectives of the Feedstock Partnership were to (1) provide a wide range of information for feedstock selection (species choice) and management practice options for a variety of regions and (2) develop national maps of potential feedstock …

Genomic Prediction Accuracy For Switchgrass Traits Related To Bioenergy Within Differentiated Populations, Jason D. Fiedler, Christina Lanzatella, Serge J. Edme, Nathan A. Palmer, Gautam Sarath, Rob Mitchell, Christian M. Tobias

Department of Agronomy and Horticulture: Faculty Publications

Background: Switchgrass breeders need to improve the rates of genetic gain in many bioenergy-related traits in order to create improved cultivars that are higher yielding and have optimal biomass composition. One way to achieve this is through genomic selection. However, the heritability of traits needs to be determined as well as the accuracy of prediction in order to determine if efficient selection is possible.

Results: Using five distinct switchgrass populations comprised of three lowland, one upland and one hybrid accession, the accuracy of genomic predictions under different cross-validation strategies and prediction methods was investigated. Individual genotypes were collected using GBS …

Phytoplankton Dynamics And Structure, And Ecological Status Estimation By The Q Assemblage Index: A Comparative Analysis In Two Shallow Mediterranean Lakes, Tuğba Ongun Sevi̇ndi̇k, Hati̇ce Tunca, Ari̇f Gönülol, Ni̇sa Gürsoy, Şeyma Nur Küçükkaya, Zuhal Kinali

Turkish Journal of Botany

The aim of this research was to compare the phytoplankton structure on the basis of environmental variables and test the Q assemblage index based on phytoplankton functional groups in two shallow Mediterranean lakes [Lake Taşkısığı (LT) and Lake Little Akgöl (LLA)] in the north of Turkey. Variations in the phytoplankton structure and environmental parameters were analyzed monthly at two stations of each lake between January 2013 and December 2013. We showed that total phosphorus (TP) values were vital in shaping phytoplankton biomass in eutrophic LLA. Nitrate-nitrogen (NO3-N), TP, zmix/zeu, and macrophyte coverage are important in shaping the differences in dominant …

Plant Biomass: Insights From Imaging Cell Walls Using Molecular Probes, Utku Avci

Turkish Journal of Botany

Cell walls are an important and growing subject of research in plant biology. In the last decade, there has been an increasing interest in using cell walls as feedstock for the production of second generation biofuels. This has resulted in an expansion in the number of cell wall studies and has highlighted the need for a better understanding of cell wall structure and function. Cell walls are composed of polymers with complex and dynamic structures that vary between cell types and developmental stages as well as between taxonomic groups of plants. To address the diversity of glycans that form the …

Long-Term Impacts Of Fuel Treatments On Tree Growth And Aboveground Biomass Accumulation In Ponderosa Pine Forests Of The Northern Rocky Mountains, Kate A. Clyatt

Graduate Student Theses, Dissertations, & Professional Papers

In western North America, many low-elevation, dry forest types historically experienced frequent, low-severity fires. However, European settlement and fire suppression policies have contributed to over a century of fire exclusion, substantially altering forest structure and composition. There is considerable interest in restoring fire resilient characteristics to these forests through fuel reduction treatments. One limitation of current research on the impacts of fuel treatments is treatment longevity, as few studies have been able to quantify long-term responses to commonly applied treatments. This research evaluated tree growth and aboveground biomass responses 23 years after treatment in two silvicultural installations with different underburning …

Estimating Herbaceous Biomass Of Grassland Vegetation Using The Reference Unit Method, Eric D. Boyda, Jack L. Bulter, Lan Xu

The Prairie Naturalist

Aboveground net primary production provides valuable information on wildlife habitat, fire fuel loads, and forage availability. Aboveground net primary production in herbaceous plant communities is typically measured by clipping aboveground biomass. However, the high costs associated with physically harvesting plant biomass may prevent collecting sufficient data to account for natural spatial and temporal variability of vegetation at a landscape scale. Various double-sampling techniques have been developed to increase sample size while reducing cost. We applied a biomass estimation technique previously developed for estimating shrub biomass using representative samples or “reference units” to estimate herbaceous grassland biomass. Our reference units consisted …

Influence Of Culture Media And Carbon Sources On Biomass Productivity And Oil Content Of The Algae Sirogonium Sticticum, Temnogyra Reflexa, Uronema Elongatum, And Chroococcus Turgidus, Aftab Alam, Saleem Ullah, Sahib Alam, Hamid Ullah Shah, Saadia Aftab, Muhammad Siddiq, Nazish Manzoor

Turkish Journal of Botany

The aim of the present study was to investigate the effect of nutritional formulations, viz. Bristol's medium (BM), water extract of dairy waste (DW), commercial fertilizers mix (FM), and carbon sources such as NaHCO3, CaCO3, and wheat flour hydrolysate on the biomass productivity and oil content of the algal species Sirogonium sticticum, Temnogyra reflexa, Uronema elongatum, and Chroococcus turgidus. Among the nutritional formulations tested, BM showed the highest average effect, followed by DW, on the biomass and oil contents of the species. As for carbon sources, CaCO3 was more efficient in enhancing algal biomass, while NaHCO3 was proficient in increasing …

Photosynthetic Gas Exchange, Chlorophyll Fluorescence, Antioxidant Enzymes, And Growth Responses Of Jatropha Curcas During Soil Flooding, Krishan Kumar Verma, Munna Singh, Ramwant Kumar Gupta, Chhedi Lal Verma

Turkish Journal of Botany

The response of chlorophyll fluorescence, photosynthetic CO_2 assimilation (PN), stomatal conductance (gs), electrolyte leakage, and transpiration (E) was observed in Jatropha curcas seedlings subjected to soil flooding. A strong reduction in growth, leaf-area expansion (64%), and stomatal conductance (45%) impaired photosynthetic CO_2 assimilation (66%), which eventually reduced biomass yield. The ratio between variable-to-initial chlorophyll fluorescence (Fv/Fo) and the maximum quantum yield efficiency of the photosystem II (Fv/Fm) was used to explore damage associated with the functioning of the photosynthetic apparatus. A strong, non-linear correlation between physiological parameters and soil flooding duration was found. Our study primarily revealed consequences of epigenetics, …

Grass And Canada Goldenrod (Solidago Canadensis) Competition And Implications For Management In The Northern Tallgrass Prairie, Alexander J. Smart, Gary E. Larson, Peter J. Bauman

The Prairie Naturalist

Canada goldenrod (Solidago canadensis L.) is a native perennial forb that can form dense clonal patches and become weedy in pastures of the Northern Tallgrass Prairie Ecoregion. Our objectives were to determine 1) the competitive effects between Canada goldenrod and grass, 2) the relationship between Canada goldenrod stem density and grass biomass, and 3) the distribution of Canada goldenrod stem density at the pasture scale. We used regression analysis to develop a relationship between Canada goldenrod stem density and grass biomass. Additionally, we estimated the frequency distribution of Canada goldenrod stem density categories using three evenly distributed 100 × 100-m …

Effects Of Species, Water, And Nitrogen On Competition Among Three Prairie Grasses, Jerry L. Weatherford, Randall W. Myster

The Prairie Naturalist

We conducted an experiment to investigate effects of species, water (W), and nitrogen (N) on competition among little bluestem (Schizachyrium scoparium), sideoats grama (Bouteloua curtipendula), and indiangrass (Sorghastrum nutans). All biomass parameters and the root:shoot ratio of little bluestem were reduced by the presence off 1 of 2 other species, and its shoot biomass and total biomass were both increased by addition of N. Root and shoot biomass of sideoats grama were reduced by the presence of indiangrass and its total biomass was reduced by the presence of itself, whereas its shoot biomass was increased by addition of W at …

Winterkill And Biomass Of The Painted Turtle In A South Dakota Wetland, Steven G. Platt, Zannita Fast Horse, Warren Cross, Sylvio Mannel, Thomas R. Rainwater

The Prairie Naturalist

Winterkill occurs when drought conditions expose hibernating turtles to desiccation and lethaly cold temperatures. Winterkill is thought to represent a major source of mortality in northern populations of the painted turtle (Chrysemys picta), but few field observations are available. We herein reported on catastrophic winterkill among western painted turtle (C. pieta bellii) at Limestone Butte Lake (LBL) in western South Dakota during the winter of 2003-2004. Additionally, we used the carcasses of winterkilled turtles (n = 86) to estimate the standing crop biomass of the painted turtle at LBL (0.6 kg/ha). This was the only estimate …

Chemical Composition And Response To Dilute-Acid Pretreatment And Enzymatic Saccharification Of Alfalfa, Reed Canarygrass, And Switchgrass, Bruce S. Dien, Hans-Joachim G. Jung, Kenneth P. Vogel, Michael D. Casler, Joann F. S. Lamb, Loren Iten, Robert C. Mitchell, Gautum Sarath

Department of Agronomy and Horticulture: Faculty Publications

Alfalfa stems, reed canarygrass, and switchgrass; perennial herbaceous species that have potential as biomass energy crops in temperate regions; were evaluated for their bioconversion potential as energy crops. Each forage species was harvested at two or three maturity stages and analyzed for carbohydrates, lignin, protein, lipid, organic acids, and mineral composition. The biomass samples were also evaluated for sugar yields following pretreatment with dilute sulfuric followed by enzymatic saccharification using a commercial cellulase preparation. Total carbohydrate content of the plants varied from 518 to 655 g kg^-1 dry matter (DM) and cellulose concentration from 209 to 322 g kg …

Germination, Growth And Biomass Accumulation As Influenced By Seed Size In Mesua Ferrea L., A. Arunachalam, M. L. Khan, N. D. Singh

Turkish Journal of Botany

Mesua ferrea L. was evaluated for its germination, seedling growth and biomass across four seed size classes. The production of two- and three-seeded fruits was high. The viability of the seeds was 58-81%. Germination was positively correlated with seed weight. Heavier seeds showed early and rapid germination. The contribution of leaves to total biomass yield was 27-60 % in 1-month old seedlings. Carbon content was also related with plant length and weight in the seedlings. The study concludes that the variations in seed size have a substantial influence on growth and biomass accumulation in Mesua ferrea. Such variation also helps …

Macrofungi Of Yahyalı Region (Kayseri), Giyasetti̇n Kaşik, Celaleddi̇n Öztürk, Azi̇z Türkoğlu, Hasan Hüseyi̇n Doğan

Turkish Journal of Botany

Some macrofungi specimens were collected from Yahyalı (Kayseri) province in 2000-2001. As a result of field and laboratory studies, 94 taxa belonging to 28 families were identified. Nine species belong to Ascomycotina and 85 to Basidiomycotina. The distribution, habitat and collecting numbers of the identified species are given.

A Study On The Occurrence Of Merismopedia Meyen (Cyanobacteria) Populations On The Littoral Sediments Of İzmit Bay (Turkey), Yelda Aktan, Güler Aykulu

Turkish Journal of Botany

In a previous study of epipelic diatoms on the littoral sediments of İzmit Bay (Marmara Sea, Turkey), which was carried out from March 1999 to September 2000, it was found that two Merismopedia Meyen species (Cyanobacteria) occurred frequently. In the present study, the seasonal variations of density and biomass of the Merismopedia species, which were dominant at certain times in the epipelic algal flora on the littoral sediments of İzmit Bay, were investigated. In addition, some physical and chemical parameters were measured. One of the species, Merismopedia glauca (Ehrenb.) Nägeli, was dominant in terms of cell numbers and frequency while …

Seasonal Variations In The Biomass Of Macro-Algal Communities From The Gulf Of Antalya (North-Eastern Mediterranean), İsmai̇l İbrahi̇m Turna, Ö. Osman Ertan, Mario Cormaci, Giovanni Furnari

Turkish Journal of Botany

In this study, the seasonal variations in the macro-algal biomass in the Gulf of Antalya (north-eastern Mediterranean) were investigated. The seasonal variations of the macro-algal biomass were found to be related to the biology of the abundant species during less favourable seasons. These variations occurred usually as a result of a significant reduction in algal density [Padina pavonica (L.) J.V.Lamour., Dasycladus vermicularis (Scopoli) Krasser, Chondrophycus papillosus (C.Agardh) Garbay & Harper] or loss of major part of their thallus [Cystoseira spp., Liagora distenta (Mertens ex Roth) J.V.Lamour., Liagora viscida (Forssk.) C.Agardh]. The highest values of biomass were found to be in …

Using Upper Boundary Constraints To Quantify Competitive Response Of Desert Annuals, Laura M. Lessin, Andrew R. Dyer, Deborah E. Goldberg

Faculty Publications

Using a target-neighborhood approach with six annual dicot species in the Negev Desert of Israel, we tested whether neighborhood biomass constrained the upper limit of plant performance and if the slope of the upper boundary was correlated to species trait means, such as relative growth rate (RGR) and seed mass. Target individuals were measured in early spring and then collected at the onset of the dry season along with all naturally occurring neighbors within a 5-cm radius of the target. Using a minimum of 50 samples for each of the six target species, we found no significant relationships between target …

Fine Root Biomass Distribution And Production Along A Barrier Island Chronosequence, Mark Jeffrey Stevenson

Biological Sciences Theses & Dissertations

Fine roots play an important role in community development on barrier islands. Fine roots can influence nutrient losses from the soil environment along with regulating water loss based on their distribution and concentration (Nobel et al., 1989; Gleeson and Tilman, 1990). A sequential coring method was used to determine fine root biomass and total biomass along a chronosequence of barrier island dunes and swales on the Virginia Coast Reserve - Long Term Ecological Research Site. Fine root production and the effects of nitrogen fertilization were also examined using an ingrowth core method along the chronosequence. Live fine root biomass, total …