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Purification And Partial Characterisation Of An Acid Lipase In Germinating Lipidbody Linseedlings, Reda Helmy Ahmed Sammour
Purification And Partial Characterisation Of An Acid Lipase In Germinating Lipidbody Linseedlings, Reda Helmy Ahmed Sammour
Turkish Journal of Botany
Electrophoretic analysis of germinating linseed proteins showed a gradual decrease in the quantity of a protein with a molecular weight of 42 kDa. This protein accumulates after 36 h of germination in synchronisation with an increase in lipase activity, and a decrease in the quantity of the total lipids. The 42 kDa subunit was found to be a lipid body membrane protein. This protein was isolated and identified by immunoprecipitation as a subunit of lipase. The linseed lipase acted on a wide range of triacylglycerols and had optimal activity at pH 4.7. The activity of the enzyme was slightly affected …