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Botany

University of Nebraska - Lincoln

Open Access Master's Theses (through 2010)

Series

1962

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Full-Text Articles in Life Sciences

The Effects Of Plant Virus Purification Procedures On Extracts Of Healthy Plants, Sue Ann Tolin Aug 1962

The Effects Of Plant Virus Purification Procedures On Extracts Of Healthy Plants, Sue Ann Tolin

Open Access Master's Theses (through 2010)

This study was undertaken to devise an easy, accurate method to detect and separate various components in extracts of healthy bean, tobacco, and barley plants by using techniques common to virus purification and to determine the effect of some of the more common denaturing and adsorbing agents on these healthy plant components.Conditions for density-gradient centrifugation were selected to give the best separation of normal components, and these conditions were then used as the basis of an analytical method to evaluate the denaturation procedures.

Protoplasmic components of healthy bean, tobacco, and barley plants were isolated and studied by use of techniques …