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Biotechnology

Micropropagation

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Micropropagation Of Grammatophyllum Multiflorum Var. 'Tigrinum' Lindley And Evaluation Of Its Antimicrobial Activities, Eufrocinio Marfori Dec 2022

Micropropagation Of Grammatophyllum Multiflorum Var. 'Tigrinum' Lindley And Evaluation Of Its Antimicrobial Activities, Eufrocinio Marfori

The Philippine Agricultural Scientist

Grammatophyllum multiflorum var. ‘tigrinum’ Lindley is an endangered orchid species endemic to the Philippines. To ensure its sustainable utilization as a source of phytochemicals, the types and concentrations of cytokinin, and auxin for shoot and root induction using nodal explants from in vitro germinated seedlings for its mass propagation were established. Seeds were germinated in vitro using MS medium without growth regulator. The nodal explants from the in vitro germinated seedlings were placed in MS medium containing different cytokinins and auxins to optimize multiple shoots and root formation, respectively. Thidiazuron (TDZ) at 2.5 μM was found to be the best …

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Physicochemical And Spectroscopic Characterization Of Biofield Energy Treated Gerbera Multiplication Medium, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana Nov 2015

Physicochemical And Spectroscopic Characterization Of Biofield Energy Treated Gerbera Multiplication Medium, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

Mahendra Kumar Trivedi

The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi’s biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis, surface area analysis, thermogravimetric analysis (TGA), elemental analysis, …

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Effect Of A Biofield Treatment On Plant Growth And Adaptation (Benth.), Gopal Nayak, Nandini Altekar Apr 2015

Effect Of A Biofield Treatment On Plant Growth And Adaptation (Benth.), Gopal Nayak, Nandini Altekar

Mahendra Kumar Trivedi

Quantum mechanics was developed when human energies of consciousness were found to influence observations at the scale of elementary particles, here referred as non-contact biofield treat-ment or biofield energies. Quantum mechanics has also proved ef-ficacious in biological processes. The present experiments found an enhanced and significant impact of the biofield treatment on adaptive micropropagation response and callus induction of two plant spe-cies, Withania somnifera and Amaranthus dubius. The enhancement was perhaps due to greater focus on adaptation rather than specific mechanisms, showing high potential including at biochemical and genetic levels. Possible reasons for the enhancement are discussed and a possible …

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Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati Oct 2013

Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati

Rajesh Pati

Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for …

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In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati Oct 2013

In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati

Rajesh Pati

Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).

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Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, Rosna Binti Mat Taha Jan 2011

Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, Rosna Binti Mat Taha

Rosna Binti Mat Taha

The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid …

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In Vitro Clonal Propagation Of Bael (Aegle Marmelos Corr.) Cv. Cishb1 Through Enhanced Axillary Branching, Rajesh Pati Oct 2008

In Vitro Clonal Propagation Of Bael (Aegle Marmelos Corr.) Cv. Cishb1 Through Enhanced Axillary Branching, Rajesh Pati

Rajesh Pati

Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10-15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. The micro shoots were rooted (100 %) on ½ strength MS medium supplemented with IBA 49.0 + IAA 5.7 …

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In Vitro Plant Regeneration From Mature Explant Of Aegle Marmelos Corr. Cv. Cish-B2, Rajesh Pati Oct 2008

In Vitro Plant Regeneration From Mature Explant Of Aegle Marmelos Corr. Cv. Cish-B2, Rajesh Pati

Rajesh Pati

No abstract provided.

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