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In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
Turkish Journal of Biology
A quantitative test to study Listeria and Salmonella adherence to epithelial cells was developed. The quantitative test is rapid and allows the simultaneous testing of many variables such as the adherence ability of different bacteria to their target cells as well as the capability of various molecules to inhibit bacterial adherence. By using a strain-specific standard curve in each test, the test for the quantification of adherent bacteria became specifically sensitive. Non-viable biotinylated bacteria and immobilized cells or their extract were found relevant for the study of bacteria-epithelial cell interactions. Adherence ability of Listeria strains was found to be 10-fold …