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Articles 1 - 30 of 48
Full-Text Articles in Life Sciences
Expression Of A Fungal Cellulase Gene By \Beta-Glucanase Promoter Of Streptococcus Bovis, Mehmet Sai̇t Eki̇nci̇
Expression Of A Fungal Cellulase Gene By \Beta-Glucanase Promoter Of Streptococcus Bovis, Mehmet Sai̇t Eki̇nci̇
Turkish Journal of Biology
The promoter region of the §-glucanase gene was identified using a transcriptional fusion between the upstream region of the Streptococcus bovis \beta-glucanase gene and the celA gene. Using the transcriptional and protein localisation signals of the S. bovis \beta-glucanase gene, an in-frame tarnslational fusion between the end of the \beta-glucanase signal sequence and the ATG of the Neocallimastix patriciarum celA gene was constructed. The \beta-glucanase promoter-celA fusion was expressed in both E. coli and S. bovis. The activity of the protein produced was found to be cell-associated in E. coli, it but localised to the supernatant fraction of harvested cells …
Nucleotypic Effects In Different Genotypes Of Vicia Sativa L., Serap Çeli̇kler, Rahmi̇ Bi̇laloğlu
Nucleotypic Effects In Different Genotypes Of Vicia Sativa L., Serap Çeli̇kler, Rahmi̇ Bi̇laloğlu
Turkish Journal of Biology
Seven cultivar varieties of Vicia sativa L. were used. Nucleotypic effects of telophase (2C) nuclear DNA amounts on seed weight, percent mitotic index, stomatal frequency/mm^2, chlorophyll a and b content, root growth and shoot growth were determined. No correlation was observed between the DNA contents and the percent mitotic index, chlorophyll a and b content, or root growth of the cultivar varieties. However a significant correlation was observed between DNA content and the seed weight, stomatal frequency and shoot growth of the cultivar varieties.
Viral Control Of The European Pine Sawfly, Neodiprion Sertifer (Geoffroy) In Turkey, Mustafa Yaman, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Viral Control Of The European Pine Sawfly, Neodiprion Sertifer (Geoffroy) In Turkey, Mustafa Yaman, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The European pine sawfly, Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae), is a serious pest of pine plantations and ornamentals. Chemical pesticides used to control this pest have a detrimental effect on the environment. The Neodiprion sertifer nuclear polyhedrosis virus (NsNPV) frequently causes considerable decreases in the populations but doses not harm other animals or plants. In this study, we tested five different concentrations of NsNPV against the larvae of N. sertifer and showed viral infection using Giemsa staining. When a viral concentration of 5x10^9 PIB/ml (Polyhedral Inclusion Body) was utilized, larval mortality started on the fourth day and reached 90.4% on …
Assessment Of Polymorphic Aflp Markers In Triticum Durum And Aegilops Sp., Aytül İnci̇rli̇, Hati̇ce Bi̇lgi̇ç, Mahi̇nur S. Akkaya
Assessment Of Polymorphic Aflp Markers In Triticum Durum And Aegilops Sp., Aytül İnci̇rli̇, Hati̇ce Bi̇lgi̇ç, Mahi̇nur S. Akkaya
Turkish Journal of Biology
Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. In addition, the reproducibility of the AFLP marker patterns is much more dependent on the quality and the purity of the DNA samples to be processed. The present study describes the optimization of reaction conditions and determination of the most polymorphic selective amplification primer sets in a comparison of winter and spring type Turkish durum wheat varieties. AFLP products resulted in about 100 bands, and the number of polymorphic AFLP loci …
Cellular Adhesion And Adhesion Molecules, Zerri̇n Seller
Cellular Adhesion And Adhesion Molecules, Zerri̇n Seller
Turkish Journal of Biology
In recent years, cell adhesion and cell adhesion molecules have been shown to be important for many normal biological processes, including embryonic cell migration, immune system functions and wound healing. It has also been shown that they contribute to the pathogenesis of a large number of common human disorders, such as rheumatoid arthritis and tumor cell metastasis in cancer. In this review, the basic mechanisms of cellular adhesion and the structural and functional features of adhesion molecules are summarized.
Blood Serum Proteins Of Apodemus Flavicollis And Apodemus Hermonensis (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Ercüment Çolak, Nuri̇ Yi̇ği̇t, Mustafa Sözen, Şaki̇r Özkurt
Blood Serum Proteins Of Apodemus Flavicollis And Apodemus Hermonensis (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Ercüment Çolak, Nuri̇ Yi̇ği̇t, Mustafa Sözen, Şaki̇r Özkurt
Turkish Journal of Biology
The globulin, albumin, postalbumin, and prealbumin proteins of Apodemus flavicollis and Apodemus hermonensiswere examined by SDS-PAGE. In A. flavicollis and A. hermonensis respectively, 6-9 and 7-9 electrophoretic bands were determined in the globulin region, respectively. Both species have one band in the postalbumin zone, and two bands in the prealbumin zone. A. flavicollis differs from A. hermonensisin terms of postalbumin.
Isolation And Characterization Of Pediocin Producing Pediococcus Pentosaceus Pep1 From Vacuum-Packed Sausages, Özlem Osmanağaoğlu, Yavuz Beyatli, Ufuk Gündüz
Isolation And Characterization Of Pediocin Producing Pediococcus Pentosaceus Pep1 From Vacuum-Packed Sausages, Özlem Osmanağaoğlu, Yavuz Beyatli, Ufuk Gündüz
Turkish Journal of Biology
Pediococcus pentosaceus Pep1 was isolated from vacuum-packed sausages, and it was shown to produce a potentially novel antimicrobial agent active against several species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Bacillus and Listeria, many of which are associated with food spoilage and health hazards of food origin. Gram-negative organisms such as Yersinia enterocolitica and Escherichia coli were not inhibited by this antimicrobial agent. Since the antimicrobial agent was found to be sensitive to proteolytic enzymes, resistant to heat and organic solvents and active over a wide range of pH values, between 3 and 8, apparently most stable in the lower …
Transient Eexpression Of B-Glucuronidase Reporter Gene In Sainfoin (Onobrychis Viciifolia Scop.) Cotyledons Via Microprojectile Bombardmen, Sertaç Önde, Cengi̇z Sancak, Suzan Altinok, Melahat Bi̇rsi̇n, Murat Özgen
Transient Eexpression Of B-Glucuronidase Reporter Gene In Sainfoin (Onobrychis Viciifolia Scop.) Cotyledons Via Microprojectile Bombardmen, Sertaç Önde, Cengi̇z Sancak, Suzan Altinok, Melahat Bi̇rsi̇n, Murat Özgen
Turkish Journal of Biology
Transient \beta-glucuronidase reporter gene (GUS) expression was visualised in whole cotyledons of sainfoin (Onobrychis viciifolia Scop.) after microprojectile bombardment with pBI221.23 DNA. Optimum rupture disk pressure and sample plate distance were determined to be 1350 psi and 6 cm, respectively. Mean gold particle diameter of 1.0 µm and 48 h post-bombardment histochemical GUS assay was found to be superior to 1.6 µm gold particles and 120 h post-bombardment GUS assay. Microscopic analysis revealed the localisation of the gold particles predominantly in the epidermal cell layer, whereas less than 1% of the particles were localised in sub-epidermal cells, as deep as …
Random Amplified Polymorphic Dna (Rapd) Markers, Fevzi̇ Bardakci
Random Amplified Polymorphic Dna (Rapd) Markers, Fevzi̇ Bardakci
Turkish Journal of Biology
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplification products of anonymous DNA sequences using single, short and arbitrary oligonucleotide primers, and thus do not require prior knowledge of a DNA sequence. Low expense, efficiency in developinga large number of DNA markers in a short time and requirement for less …
Determination Of Some Fungal Metabolite As Influenced By Temperature, Time, Ph And Sugars By Bioassay Method, Meti̇n Diğrak, Sami̇ Özçeli̇k
Determination Of Some Fungal Metabolite As Influenced By Temperature, Time, Ph And Sugars By Bioassay Method, Meti̇n Diğrak, Sami̇ Özçeli̇k
Turkish Journal of Biology
This study investigated the fungal biomass and inhibition of Bacillus megaterium DSM 32 with extracts of Fusarium equiseti, Penicillium italicum, Penicillium digitatum, Aspergillus candidus and Aspergillus parasiticus in modified Czapek Dox Broth (mCDB) as affected by temperature (6, 15, 25 °C), incubation time (up to 4 wk), pH (3.0, 5.0 and 8.0) and glucose the and fructose (4.0, 6.0 and 9.0%). The biomass production was greatest at 25 °C. In general, biomass production was greatest in media containing an addition of 4.0% glucose or 6.0% fructose; however, bacterial inhibition production was reduced by elevated sugars in mCDB over a 4-wk …
Determination Of The Dna And Amino Acid Sequences Of The Lactate Dehydrogenase Gene From Plasmodium Falciparum Strains K1 And Pf Fcbr: A Route To The Design Of New Antimalarials, Di̇lek Turgut Balik, J. John Holbrook
Determination Of The Dna And Amino Acid Sequences Of The Lactate Dehydrogenase Gene From Plasmodium Falciparum Strains K1 And Pf Fcbr: A Route To The Design Of New Antimalarials, Di̇lek Turgut Balik, J. John Holbrook
Turkish Journal of Biology
This paper describes cloning of the gene coding for the lactate dehydrogenase (LDH) gene from the two different strains of Plasmodium falciparum, K1 and PF FCBR. The DNA sequences of LDH genes of these two strains were found to be identical. Amino acid sequence alignment of LDH from P. falciparum strains K1 and PF FCBR (PfLDH) to some other known LDH sequences showed that PfLDH has 29% residue identities with Bacillus stearothermophilus (BsLDH) and 29%, 31%, 33% with dogfish, man-M_4, and pig-M_4 LDHs, respectively. It was also shown that PfLDH has insertions and single amino acid deletions. Two deletions are …
The Role Of P13-Kinase On Melanoma Cell Adhesion And Migration, Zerri̇n Seller, Ian R. Hart
The Role Of P13-Kinase On Melanoma Cell Adhesion And Migration, Zerri̇n Seller, Ian R. Hart
Turkish Journal of Biology
Integrins are responsible for anchoring cells to the extracellular matrix and also can initiate intracellular signal pathways. The interaction between integrins and the extracellular matrix results in activation of PKC, PTKs, p21Ras, MAPK and PI3-kinase. The \alpha4\beta1 integrins are expressed mainly on haematopoietic cells and also appear on several tumour types. The adhesion of \alpha4\beta1 to VCAM-1 is an essential step for lymphocyte extravasation during the inflammatory response. Furthermore, the interaction of \alpha4\beta1, expressed on tumour cells, with VCAM-1, might have a role in tumour cell extravasation. We examined the role of PI3-kinase activation in \alpha4\beta1-mediated melanoma cell adhesion and …
The Effect Of Paraquat On The Activity Of Some Enzymes In Different Tissues Of Mice (Mus Musculus - Swiss Albino), Egemen Dere, Fi̇kri̇ye Polat
The Effect Of Paraquat On The Activity Of Some Enzymes In Different Tissues Of Mice (Mus Musculus - Swiss Albino), Egemen Dere, Fi̇kri̇ye Polat
Turkish Journal of Biology
The effects of the LD_{30} (20 mg.kg^{-1}) dose of paraquat, a herbicide, were investigated on creatine kinase (CK), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT) activities in the liver, kidney and lung of mice after 2, 4, 8, 16, 32, 64 and 72 hours. After paraquat was given intraperitoneally to Mus musculus Swiss albino mice, the control groups were injected with physiological saline. The results were evaluated using the autoanalyzer as U/L. Even though significant increases in the liver tissue LDH and GGT, kidney tissue CK and lung tissue CK and …
Oxylipin Pathway In The Biosynthesis Of Fresh Tomato Volatiles, Emi̇n Yilmaz
Oxylipin Pathway In The Biosynthesis Of Fresh Tomato Volatiles, Emi̇n Yilmaz
Turkish Journal of Biology
Fresh tomato volatiles are formed in intact fruit during ripening and upon tissue disruption. There are different pathways involved in the biosynthesis of these volatiles. The oxylipin pathway uses free unsaturated fatty acids with the sequential action of lipoxygenase, hydroperoxide lyase and alcohol dehydrogenase to produce volatile aldehyde and alcohol compounds. Oxylipin volatiles are the most important components in fresh tomato aroma. In order to genetically improve the quality of tomato aroma, the biochemistry of aroma synthesis must be studied thoroughly. This review examines current knowledge of the subject and opens new areas for further investigation.
The Evaluation Of Alkalitolerant-Mesophilic Streptomyces By Gas Chromatography And Pyrolysis Mass Spectrometry, Nevzat Şahi̇n, Kami̇l Işik, Ergi̇n Kari̇ptaş, Michael Goodfellow
The Evaluation Of Alkalitolerant-Mesophilic Streptomyces By Gas Chromatography And Pyrolysis Mass Spectrometry, Nevzat Şahi̇n, Kami̇l Işik, Ergi̇n Kari̇ptaş, Michael Goodfellow
Turkish Journal of Biology
Thirty-eight alkalitolerant-mesophilic Streptomyces, defined by Saddler in his numerical taxonomic study, were evaluated by gas chromotography and pyrolysis mass spectrometry. Group I Streptomyces sp. AK409 and group II Streptomyces sp. AK029 selected duplicated strains were clustered in their own groups. It was observed that the results of gas chromatography and pyrolysis mass spectrometry was in good congruance with the numerical taxonomic results. Therefore, it can be concluded that the different chemotaxonomic methods provide rapid and reproducible information for classifying biotechnologically significant Streptomyces strains.
Nitric Oxide Synthase In The Skeletal Muscle And Arterioles Of Rats With Streptozotosin-Induced Diabetes Mellitus, Ci̇han Demi̇rci̇, Kadri̇ye Akgün Dar, İlhan Uyaner
Nitric Oxide Synthase In The Skeletal Muscle And Arterioles Of Rats With Streptozotosin-Induced Diabetes Mellitus, Ci̇han Demi̇rci̇, Kadri̇ye Akgün Dar, İlhan Uyaner
Turkish Journal of Biology
Nitric oxide synthase (NOS) that uses NADPH as a cofactor is an enzyme which produces nitric oxide (NO) from L-arginine. Endothelial-derived (eNOS), inducible (iNOS) and neuronal (nNOS) nitric oxide synthase are 3 known isoforms. Endothelial NO regulates the vascular tonus and causes the dilatation of vessels, while NOS localized in the sarcolemma of striated muscle plays a role in the regulation of muscle hemodynamics. Many authors have found differences in NO production in some pathological conditions such as diabetes mellitus (DM), hypertension and atherosclerosis. In this study, NADPH-diaphorase (NADPH-d) was histochemically employed to explore any changes in NO production associated …
In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
Turkish Journal of Biology
A quantitative test to study Listeria and Salmonella adherence to epithelial cells was developed. The quantitative test is rapid and allows the simultaneous testing of many variables such as the adherence ability of different bacteria to their target cells as well as the capability of various molecules to inhibit bacterial adherence. By using a strain-specific standard curve in each test, the test for the quantification of adherent bacteria became specifically sensitive. Non-viable biotinylated bacteria and immobilized cells or their extract were found relevant for the study of bacteria-epithelial cell interactions. Adherence ability of Listeria strains was found to be 10-fold …
The Cytogenetical Comparisons Of Spalax (Rodentia: Spalacidae) Populations From Middle Kızılırmak Basin, Turkey, Eşref Yüksel, Mehmet Doğan Gülkaç
The Cytogenetical Comparisons Of Spalax (Rodentia: Spalacidae) Populations From Middle Kızılırmak Basin, Turkey, Eşref Yüksel, Mehmet Doğan Gülkaç
Turkish Journal of Biology
Chromosomal polymorphism and speciation problem in genus Spalax has been investigated. All the specimens were identified as Spalax leucodon cilicicus Mehely, 1909. In according to karyotype analysis, two different populations were described from the field. The diploid number of Population A from south ant northwest of the field were found to be 60 and number of fundamental numbers were 80. The corresponding numbers in the Population B from northeast of the field were 54 and 74 respectively. In the result, the possibility of inversions in the Population B was discussed from evolutionary point of view and also the evolutionary background …
Effects Of Photoperiod And Melatonin Infusions On Body Weight In Pinealectomized Juvenile Siberian Hamsters (Phodopus Sungorus), Bülent Gündüz, Alper Karakaş
Effects Of Photoperiod And Melatonin Infusions On Body Weight In Pinealectomized Juvenile Siberian Hamsters (Phodopus Sungorus), Bülent Gündüz, Alper Karakaş
Turkish Journal of Biology
We examined the effects of daily melatonin (Mel) infusions in pinealectomized prepubertal male Siberian hamsters in three different conditions. In one study we investigated the body weight maturation response to one hour daily infusions of 10 ng, 25 ng, or 50 ng of Mel in pinealectomized hamsters. Animals received, at day 15 of life, programmed subcutaneous infusions of Mel or vehicle at one of five time points (1900-2000, 2000-2100, 2100-2200, 2400-0100, and 0300-0400 hours) for 30 days. In long-day-born (16L) animals, Mel infusion right after lights off (2000-2100 hours) significantly inhibited body growth; this dose was ineffective at other times. …
The Cytogenetic Effects Of Sodium Metabisulfite, A Food Preservative In Root Tip Cells Of Allium Cepa L., Eyyüp Rencüzoğullari, Ahmet Kayraldiz, Hasan Basri̇ İla, Tonguz Çakmak, Mehmet Topaktaş
The Cytogenetic Effects Of Sodium Metabisulfite, A Food Preservative In Root Tip Cells Of Allium Cepa L., Eyyüp Rencüzoğullari, Ahmet Kayraldiz, Hasan Basri̇ İla, Tonguz Çakmak, Mehmet Topaktaş
Turkish Journal of Biology
The effects of sodium metabisulfite (SMB) on mitosis were investigated in Allium cepa L. The roots of A. cepa were treated with SMB concentrations of 7.5 mg/lt, 15 mg/lt and 30 mg/lt for 10- and 20-hour treatment periods. SMB significantly decreased the mitotic index (MI) at all concentrations and treatment periods. While the decreasing of the MI was dose-dependent at 10 hours treatment time, SMB increased the mitotic abnormalities dose dependently.
In Situ Localization Of Germin Gene Expression During Auxin Induced Callus Formation, Mahmut Çalişkan
In Situ Localization Of Germin Gene Expression During Auxin Induced Callus Formation, Mahmut Çalişkan
Turkish Journal of Biology
Wheat embryogenic callus was induced by treating immature wheat embryos with 2,4-D. Digoxigenin-labeled germin sense and anti-sense RNA probes were prepared by in vitro transcription. By using these probes it was revealed that there was a striking induction of germin gene expression prior to visible callus formation. Although no germin gene expression was localized on the sections of immature embryos which were not incubated in callus induction medium, following 4 hours incubation in callus induction medium, germin mRNA was localized on the sections of immature embryos. Germin gene expression more conspicuous and widespread on the sections which were incubated for …
Effects Of Isochronal Exogenously Applied Synthetic-Ga_3, Fungal-Gibberellic Acid And Red Light On The Sheath Length Of The Second Leaf Of Oryza Sativa L.Cv. Tan-Ginbozu, Ş. Fati̇h Topçuoğlu, Serpi̇l Ünyayar
Effects Of Isochronal Exogenously Applied Synthetic-Ga_3, Fungal-Gibberellic Acid And Red Light On The Sheath Length Of The Second Leaf Of Oryza Sativa L.Cv. Tan-Ginbozu, Ş. Fati̇h Topçuoğlu, Serpi̇l Ünyayar
Turkish Journal of Biology
The effects of isochronal exogenously applied synthetic-GA_3, fungal-gibberellic acid and different light on the sheath length of the second leaf of Oryza sativa L.cv.Tan-ginbozu were determined under controlled environmental conditions. Our results showed that the effect of fungal-gibberellic acid on the sheath length of the second leaf is higher than that on the control groups (group of 1/2N Hoagland culture solution and group of 1/2N Hoagland culture solution+methanol); however, synthetic-GA_3 was more effective on the length of the second leaf than fungal gibberellic acid. Moreover, it was identified that the effect of red light is higher than that of fluorescent …
Effect Of Some Organophosphorus Insecticides On Soil Microorganisms, Meti̇n Diğrak, Ferdağ Kazanici
Effect Of Some Organophosphorus Insecticides On Soil Microorganisms, Meti̇n Diğrak, Ferdağ Kazanici
Turkish Journal of Biology
The effect of some organophosphorus insecticides [Isofenphos (=Oftanol DS 40), Phorate (=Thimet 10G and Foratox 10-G) and Fonofos] were investigated on soil microbiota (total viable bacteria, actinomycetes, anaerobic bacteria, aerobic endospores, proteolytic bacteria, cellülolytic microorganisms and yeast-mold). In the isofenphos-treated soil sample, the total viable bacteria number was found to be higher than that of the control groups during incubation. Moreover, it was observed that this treatment had no inhibitory effect on the development of the other microorganism groups. It was determined that the fonofos and phorate pesticides were had no inhibitory effect on the devel- opment of the soil …
Purification Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Meti̇n Yildirim
Purification Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Meti̇n Yildirim
Turkish Journal of Biology
Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a rapid and simple three-step purification procedure including freeze drying, silicic acid adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa by tricine SDS-PAGE.
Dechlorination Of Bleached Kraft Pulp By Laccase Enzyme Produced From Some White-Rot Fungi, Nazi̇f Kolankaya, Arzu Taşpinar Ünal
Dechlorination Of Bleached Kraft Pulp By Laccase Enzyme Produced From Some White-Rot Fungi, Nazi̇f Kolankaya, Arzu Taşpinar Ünal
Turkish Journal of Biology
The possibility of using crude laccase in the dechlorination of chlorine-based bleached kraft pulp was investigated. Culture supernatants of seven white-rot fungal strains and kraft-pulp samples taken from E1 (Alkaline Extraction-1), E2 (Alkaline Extraction-2) and D2 (Chlorinedioxide treatment) stages of chlorine-based bleaching processes were used as the laccase source and substrates respectively during the studies. The dechlorination activity of Trametes versicolor was found to be more than that of the other fungi examined. The addition of a laccase inducer, xylidine, into the culture medium of T. Versicolor led to an increase in dechlorination activity. In the experiment performed to find …
Characterization Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Zeli̇ha Yildirim, Meti̇n Yildirim
Characterization Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Zeli̇ha Yildirim, Meti̇n Yildirim
Turkish Journal of Biology
Lactobacillus buchneri LB isolated from a commercially fermented vegetable product produced a bacteriocin-designated buchnericin LB. It was found that buchnericin LB was heat-stable (90-121^oC for 15 min) and hydrolyzed by proteases, \alpha-chymotrypsin, trypsin, papain, proteinase K and pepsin. However, it was observed that it was resistant to catalase, peroxidase, lipase, amylases and organic solvents. Also, it was found that it retained its biological activity after exposure to pH 2.0-9.0 and after storage at -20^o and -70^oC. It was determined that buchnericin LB had a wide inhibitory spectrum, being inhibitory to the Listeria, Bacillus, Enterococcus, Micrococcus, Lactobacillus, Leuconostoc, Pediococcus, and Streptococcus …
Blood-Serum Proteins Of Rattus Rattus And Rattus Norvegicus (Mammalia: Rodentia) In Turkey, Nuri̇ Yi̇ği̇t, Reyhan Çolak, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Blood-Serum Proteins Of Rattus Rattus And Rattus Norvegicus (Mammalia: Rodentia) In Turkey, Nuri̇ Yi̇ği̇t, Reyhan Çolak, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Turkish Journal of Biology
The blood serum proteins of 35 live specimens of Rattus rattus and Rattus norvegicus collected from ten localities in Turkey were examined by SDS-PAGE (sodium dodecyl sulphate - polyacrylamide gel electrophoresis). Globulin, albumin, postalbumin, and prealbumin proteins in blood serum showed different electrophoretic patterns in populations of R. rattusand R. norvegicus.Because of the variations in electrophoretic patterns of both species, these patterns do not seem to be diagnostic characters in distinguishing R. rattus from R. norvegicus.
Effects Of Sewage Pollution The Structure Of The Community Of Ulva Lactuca, Enteremorpha Linza And Rocky Macrofauna In Dışliman Of Sinop, Levent Bat, Mehmet Akbulut, Murat Sezgi̇n, Mehmet Çulha
Effects Of Sewage Pollution The Structure Of The Community Of Ulva Lactuca, Enteremorpha Linza And Rocky Macrofauna In Dışliman Of Sinop, Levent Bat, Mehmet Akbulut, Murat Sezgi̇n, Mehmet Çulha
Turkish Journal of Biology
Untreated sewage and domestic wastes from the Sinop outfall enter the Dışliman region. The effect of this on the distribution of Ulva lactuca, Enteromorpha linza and benthic macrofauna and on enviromental parameters was determined. Increases in the growth of opportunistic green algae such as Ulva lactuca and Enteromorpha linzain response to increased nutrient supply in the Dışliman coastal ecosystems in Sinop are reported in the present study. The results of this study show that organic enrichment also increases, with Mytilus galloprovincialis (Mollusca), Erichthonius brasiliensis, Echinogammarus olivii and Amphitoes ramondi, Idotea baltica and Sphaeroma serratum(Crustacea) being the most abundant species.
Identification And Evaluation Of Clinically Significant Nocardia Brasiliensis, Nocardia Farcinica And Nocardia Otitidiscaviarum Strains Using Pyrolysis Mass Spectrometry (Pyms), Kami̇l Işik, Ergi̇n Kari̇ptaş, Nevzat Şahi̇n, Alan C. Ward, Micheal Goodfellow
Identification And Evaluation Of Clinically Significant Nocardia Brasiliensis, Nocardia Farcinica And Nocardia Otitidiscaviarum Strains Using Pyrolysis Mass Spectrometry (Pyms), Kami̇l Işik, Ergi̇n Kari̇ptaş, Nevzat Şahi̇n, Alan C. Ward, Micheal Goodfellow
Turkish Journal of Biology
Out of a total of thirty-nine Nocardia strains, eight species of N. brasiliensis, seventeen species of N. farcinica and fourteen species of N. otitidiscaviarum were identified using Pyrolysis mass spectrometry. N428, N477 N. brasiliensis; N669, N233 N. farcinica; and N231, N232 N. otitidiscaviarum duplicated strains were clustered in their own groups. Strains belonging to Nocardia brasiliensis, Nocardia farcinica and Nocardia otitidiscaviarum formed distinct pyrogroups corresponding to clusters defined in the numerical study. It can be concluded that PyMS provides a rapid and reproducible method of evaluating the taxonomic significance of Nocadia species.
Physiological Studies On The Plant Cell Wall Degrading Enzymes Of The Rumen Bacterium Ruminococcus Flavefaciens, Emi̇n Özköse, Mehmet Sai̇t Eki̇nci̇, Numan Özcan
Physiological Studies On The Plant Cell Wall Degrading Enzymes Of The Rumen Bacterium Ruminococcus Flavefaciens, Emi̇n Özköse, Mehmet Sai̇t Eki̇nci̇, Numan Özcan
Turkish Journal of Biology
Two forms of Ruminococcus flavefaciens, the wild type and a trained culture of strain 17 were compared for their cellulose and xylan degradative ability when then were grown in ryegrass, cellobiose and xylan for different incubation times. No cell-associated corboxymethylcellulase (CMCase) activity was observed after 1-day incubation in cellobiose, as no supernatant activity was detected after 1 and 3 days incubation in xylan and 2 days incubation in cellobiose grown culture. Both CMCase and xylanase activities of trained culture were higher than those of wild type culture when they were grown in ryegrass. Wild type cultures, however, showed higher levels …