Life Sciences Commons^™

Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler

IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt

Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …

(R1980) Effect Of Climate Change On Brain Tumor, Pardeep Kumar, Sarita Jha, Rajiv Aggarwal, Govind Kumar Jha

Applications and Applied Mathematics: An International Journal (AAM)

In this paper, we introduce a new dynamical model addressing the variation in climate condition due the presence of microorganisms. We also introduce a new dynamical model of cancer growth which includes three interactive cell populations with drug free environment, namely tumor cells, healthy host cells, and immune effector cells. In this, we considered the super growth of tumor cells. For the choice of certain parameters, both of the systems exhibit chaotic behavior. The aim of this work is to design the controller to control the chaos and to provide sufficient conditions which achieve synchronization of two non-identical systems, which …

A Identification And Isolation Of Several Bacteria From Oral Cavity Among Human In Soran City, Holem Hashm Balaky, Alaa Taha Younis Al-Hammadi

Al-Qadisiyah Journal of Pure Science

The aim of this study has been to estimate that the number of bacteria in the mouth exceeds the total number of people on the planet. In this context, scientists have discovered more than 700 different types of human oral bacteria. With such a large number of oral bacteria present, many diseases that may threaten health, especially oral health, can emerge. For this reason, this study was employed and conducted on 252 healthy people in the Soran area of the city of Erbil. Both sexes were involved and different ages were selected. Swabs taken from 226 participants gave positive growths …

Is Fecal Microbiota Transplantation A Safe And Effective Treatment For Gastrointestinal Diseases?, Shaindel Pinsky

The Science Journal of the Lander College of Arts and Sciences

Fecal microbiota transplantation (FMT) is a method of transferring feces from an individual with a healthy microbiome to a patient whose healthy gut bacteria is deficient. While this method is not a new one, it is constantly being explored and studied to determine if it can be an effective way to treat patients with different bowel diseases. The main target of most of these studies are patients with recurrent Clostridium difficile infections. Many studies were done to determine if the method is safe, and which method is most effective, as well as who can be a good donor or recipient …

Diversity And Abundance Of Soil Microbes Differ Along A Forest-Pasture Transect, Hannah Suli, Ashley Schumann, Cleo Bickley, Jasmine Rodriguez

IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt

No abstract provided.

On The Composistion Of Cymodocea Nodosa Root Exudate Under Artificial Blue, Green And Natural Light Conditions, Armand Martin, Ben Manriquez, Christian Pompa, Aaron Saper, Kyle A. Grice, Jason Bystriansky

DePaul Discoveries

Seagrasses are identified as a sentinel species: a good indicator of overall marine ecosystem health and function. At the rhizome, they are known to interact with marine bacteria by exchanging energy in the form of glucose and free amino acids secreted through root exudate in exchange for microbe-fixated nitrogen that can be utilized for plant growth. To analyze potential outcomes of possible future changes in light availability, an experiment was designed to collect and analyze the root exudate of Cymodocea nodosa under three light conditions (standard fluorescent light, blue LED, and green LED light). After 72 hours of treatment, the …

The Biophysical Study Of The Efficacy Of Various Phospholipid Membranes On Daptomycin, Chaya Shor, Tamar Itzkowitz

The Science Journal of the Lander College of Arts and Sciences

Daptomycin is an important lipopeptide antibiotic used in the treatment of systematic and life-threatening infections of the skin and underlying tissue caused by Gram-positive bacteria. Calcium and phosphatidylglycerol (PG) must be present on the target cell membrane for daptomycin’s mechanism of action to proceed. Calcium and PG also promote oligomerization, a formation that has been assumed to aid in the bactericidal process. The purpose of the experiments conducted was to understand the basic biophysical properties of membrane phospholipids as they exist in their pure and mixed monolayer forms. Furthermore, the experiments conducted attempted to discern how daptomycin penetrates the different …

The Role Of Diet In Inflammatory Bowel Diseases, Aviva Smilow

The Science Journal of the Lander College of Arts and Sciences

Inflammatory Bowel Disease encompasses two diseases, Crohn’s Disease and Ulcerative Colitis. During the last 70 years the evolving of diet in the industrialized countries has led to the utilization of foods that have been processed rather than those in their more natural original state. This increase in the processing of foods has been correlated with the more recent occurrence of metabolic diseases such as Inflammatory Bowel Disease. Patients suffering from this problem exhibit lesser amounts of anti-inflammatory bacteria such as Roseburia and Faecalibacterium and increased amounts of pro-inflammatory bacteria Escheria and Fusobacterium. Since diet directly alters the composition of the …

A Simple Guanidinium Isothiocyanate Method For Bacterial Genomic Dna Isolation, Erkan Mozi̇oğlu, Müslüm Akgöz, Candan Tamerler, Zühtü Tanil Kocagöz

Turkish Journal of Biology

A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.

Diversity Of Cultivable Bacteria Involved In The Formation Of Macroscopic Microbial Colonies (Cave Silver) On The Walls Of A Cave In Slovenia, Blagajana Herzog Velikonja, Rok Tkavc, Lejla Pašić

International Journal of Speleology

Karstic caves often support white, yellow, grey or pink microbial colonies that are termed ‘cave silver’ by speleologists. Using various sample pre-treatments and culture media, a wide variety of bacteria associated with these colonies were recovered from a cave in Slovenia, Pajsarjeva jama. Decreasing the inoculum size resulted in significant increases in viable counts, while pre-treatments had the opposite effect with the exception of microwave irradiation. While all growth media yielded viable counts, the maximal counts were observed on a low-nutrient TWA medium.

Based on the 16S rRNA gene sequence of OTU representatives, the majority of the 80 isolates examined …

Microbiological Immunocytochemistry: A Review Of Current Trends And Applications, Julian E. Beesley

Scanning Microscopy

It has been considered worthwhile to update previous reviews of microbiological immunocytochemistry in order to identify areas of current importance in this continually expanding area of research. Publications in virology, bacteriology and protozoology indicate a continued interest in immunocytochemistry. Deployment of colloidal gold techniques is almost universal in these applications. The post-embedding technique was the most widely applied technique although a few studies employed the immunonegative stain, pre-embedding and immunoreplica techniques, thereby reflecting the use of colloidal gold in all other areas of the biological sciences.

Studies On The Occurrence And Elemental Composition Of Bacteria In Freshwater Plankton, K. N. Booth, D. C. Sigee, E. Bellinger

Scanning Microscopy

The occurrence and cation content of bacteria in a eutrophic freshwater lake (Rostherne Mere, Cheshire, UK) were investigated over a one year sampling period in relation to cation changes in the lake surface water and phytoplankton.

Scanning electron microscope examination of trawl-net and filtered samples demonstrated bacterial association with Anabaena, Aphanizomenon and diatoms. Direct counts of associated and unassociated bacteria showed that increases in bacterial population relate to population decline of major algal constituents.

Spectrophotometric determination of selected cation levels in the lake water demonstrated wide fluctuations throughout the sampling period, with elevated levels of transition metals before and at …

The Electron Microscope Detection And X-Ray Quantitation Of Cations In Bacterial Cells, D. C. Sigee, M. H. El-Masry, R. H. Al-Rabaee

Scanning Electron Microscopy

Electron microscope autoradiography and X-ray microanalysis have been used for the detection and quantitation of cations in the bacterium Pseudomonas tabaci. These techniques differ in the information they provide (relating to either cation uptake or in situ levels), their applicability to different cations, their sensitivity and their spatial resolution. With uptake of ⁶³Ni²⁺, high resolution autoradiography (involving gold latensification and physical development) demonstrated a high degree of cation localisation to the central nucleoid area (glutaraldehyde-fixed cells) and within this to the constituent chromatin (acetic-alcohol preparations).

X-ray microanalysis of whole bacterial cells revealed the presence of substantial levels …

Molds And Bacteria In House Dust And Furniture Stuffing, Mary Ann Swaebly

Journal of the Minnesota Academy of Science

No abstract provided.