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Full-Text Articles in Life Sciences
Bacteria As Bioindicators: Bioassessment In The Bonneville Basin Of Utah, Mark Merkley, Russell Rader
Bacteria As Bioindicators: Bioassessment In The Bonneville Basin Of Utah, Mark Merkley, Russell Rader
Journal of Undergraduate Research
Bioassessment is the practice of using organisms to detect environmental health and integrity. Ecosystem health and integrity depend on the interaction between living organism and ecosystem function. All organisms or groups of organisms do not respond the same to environmental stress. Depending on various disturbance characteristics, each group may respond at different rates and provide different information (Rader, 2001). Besides the usual macrophytes, macroinvertebrates, or algae, bacteria have the capability of being strong bioindicators. Because of their extremely small size, bacteria have the highest surface-to volume ratios of any living organisms. Thus, bacteria should be the most environmentally aware organisms, …
A Simple Guanidinium Isothiocyanate Method For Bacterial Genomic Dna Isolation, Erkan Mozi̇oğlu, Müslüm Akgöz, Candan Tamerler, Zühtü Tanil Kocagöz
A Simple Guanidinium Isothiocyanate Method For Bacterial Genomic Dna Isolation, Erkan Mozi̇oğlu, Müslüm Akgöz, Candan Tamerler, Zühtü Tanil Kocagöz
Turkish Journal of Biology
A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.