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Full-Text Articles in Life Sciences
Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao
Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao
Michigan Tech Publications
Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations thereof. The post-processing of the end products can occur in many ways, such as cross-linking, enzyme linking, and thermal curing, to achieve enhanced chemical and physical properties. Such multi-factor tunability is very promising in applications such as tissue engineering, 3D organs/organoids, and cell differentiation. While the established methods involve the use of soluble small molecules, growth factors, stereolithography, and micro-patterning, electrospinning involves an inexpensive, labor …
In-Vitro Cell Culture Model To Determine Toxic Effects Of Soil Arsenic Due To Direct Dermal Exposure, Manas Warke, Madeline English, Laura De Marchi, Rohan Deep Sarkar, Srinivas Kannan, Rupali Datta, Smitha Rao
In-Vitro Cell Culture Model To Determine Toxic Effects Of Soil Arsenic Due To Direct Dermal Exposure, Manas Warke, Madeline English, Laura De Marchi, Rohan Deep Sarkar, Srinivas Kannan, Rupali Datta, Smitha Rao
Michigan Tech Publications
Arsenic (As) is one of the most toxic environmental pollutants, classified as a Class I carcinogen. Anthropogenic activities have led to an increase in As contamination of soils. Using animal models to study the health impacts of As is time and cost-prohibitive, hence attempts have been made to develop in-vitro cell culture models. However, most studies so far have not represented realistic environmental exposure conditions. We exposed Human Immortalized Keratinocyte (HaCaT) and Primary Human Dermal Fibroblasts (HDFa) cells to the water-soluble fraction of arsenic extracted from As spiked Immokalee soil to study the effect of soil As on skin cells. …
Activation Of Orexin System Stimulates Camkii Expression, Yuanyuan Fan, Enshe Jiang, Huanjia Gao, Jeremy Bigalke, Bojun Chen, Chunxiu Yu, Qinghui Chen, Zhiying Shan
Activation Of Orexin System Stimulates Camkii Expression, Yuanyuan Fan, Enshe Jiang, Huanjia Gao, Jeremy Bigalke, Bojun Chen, Chunxiu Yu, Qinghui Chen, Zhiying Shan
Michigan Tech Publications
Hyperactivity of the orexin system within the paraventricular nucleus (PVN) has been shown to contribute to increased sympathetic nerve activity (SNA) and blood pressure (BP) in rodent animals. However, the underlying molecular mechanisms remain unclear. Here, we test the hypothesis that orexin system activation stimulates calcium/calmodulin-dependent kinase II (CaMKII) expression and activation, and stimulation of CaMKII expressing PVN neurons increases SNA and BP. Real-time PCR and/or western blot were carried out to test the effect of orexin-A administration on CaMKII expression in the PVN of normal Sprague Dawley (SD) rats and orexin receptor 1 (OX1R) expressing PC12 cells. Immunostaining was …