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Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Expression Of The Thyroid Hormone Receptor Gene, Erbaα, In B Lymphocytes: Alternative Mrna Processing Is Independent Of Differentiation But Correlates With Antisense Rna Levels, Michelle Laura Hastings, Christine Milcarek, Kathlee Martincic, Martha L. Peterson, Stephen H. Munroe
Expression Of The Thyroid Hormone Receptor Gene, Erbaα, In B Lymphocytes: Alternative Mrna Processing Is Independent Of Differentiation But Correlates With Antisense Rna Levels, Michelle Laura Hastings, Christine Milcarek, Kathlee Martincic, Martha L. Peterson, Stephen H. Munroe
Biological Sciences Faculty Research and Publications
The erbAα gene encodes two α-thyroid hormone receptor isoforms, TRα1 and TRα2, which arise from alternatively processed mRNAs, erbAα1 (α1) and erb α2 (α2). The splicing and alternative polyadenylation patterns of these mRNAs resemble that of mRNAs encoding different forms of immunoglobulin heavy chains, which are regulated at the level of alternative processing during B cell differentiation. This study examines the levels of erbAα mRNA in eight B cell lines representing four stages of differentiation in order to determine whether regulation of the alternatively processed α1 and α2 mRNAs parallels the processing of immunoglobulin heavy chain mRNAs. Results show that …
Effect Of 17 Days Of Bed Rest On Peak Isometric Force And Unloaded Shortening Velocity Of Human Soleus Fibers, Jeffrey J. Widrick, Janell Romatowski, James L. W. Bain, Scott W. Trappe, Todd A. Trappe, Joyce L. Thompson, David L. Costill, Danny A. Riley, Robert H. Fitts
Effect Of 17 Days Of Bed Rest On Peak Isometric Force And Unloaded Shortening Velocity Of Human Soleus Fibers, Jeffrey J. Widrick, Janell Romatowski, James L. W. Bain, Scott W. Trappe, Todd A. Trappe, Joyce L. Thompson, David L. Costill, Danny A. Riley, Robert H. Fitts
Biological Sciences Faculty Research and Publications
The purpose of this study was to examine the effect of prolonged bed rest (BR) on the peak isometric force (Po) and unloaded shortening velocity (Vo) of single Ca2+-activated muscle fibers. Soleus muscle biopsies were obtained from eight adult males before and after 17 days of 6° head-down BR. Chemically permeabilized single fiber segments were mounted between a force transducer and position motor, activated with saturating levels of Ca2+, and subjected to slack length steps. Vo was determined by plotting the time for force redevelopment vs. the slack step distance. …
Expression Of Smooth Muscle Myosin Heavy Chains And Unloaded Shortening In Single Smooth Muscle Cells, Daniel P. Meer, Thomas Eddinger
Expression Of Smooth Muscle Myosin Heavy Chains And Unloaded Shortening In Single Smooth Muscle Cells, Daniel P. Meer, Thomas Eddinger
Biological Sciences Faculty Research and Publications
The functional significance of the variable expression of the smooth muscle myosin heavy chain (SM-MHC) tail isoforms, SM1 and SM2, was examined at the mRNA level (which correlates with the protein level) in individual permeabilized rabbit arterial smooth muscle cells (SMCs). The length of untethered single permeabilized SMCs was monitored during unloaded shortening in response to increased Ca2+ (pCa 6.0), histamine (1 μM), and phenylephrine (1 μM). Subsequent to contraction, the relative expression of SM1 and SM2 mRNAs from the same individual SMCs was determined by reverse transcription-polymerase chain reaction amplification and densitometric analysis. Correlational analyses between the SM2-to-SM1 …
Compounds Exuded By Phaseolus Vulgaris That Induce A Modification Of Rhizobium Etli Lipopolysaccharide, Dominik M. Duelli, K. Dale Noel
Compounds Exuded By Phaseolus Vulgaris That Induce A Modification Of Rhizobium Etli Lipopolysaccharide, Dominik M. Duelli, K. Dale Noel
Biological Sciences Faculty Research and Publications
Exudates released from germinating seeds and roots of a black-seeded bean (Phaseolus vulgaris cv. Midnight Black Turtle Soup) induce an antigenic change in the lipopolysaccharide (LPS) of Rhizobium etli CE3. By spectroscopic analyses and chromatographic comparisons with derived standards, the chemical structures of the aglycone portions of the major inducing molecules from seed exudate were deduced, and they were identified as delphinidin, cyanidin, petunidin, and malvidin. These anthocyanidins were present in seed exudate mainly as glycosides, the chief inducer being delphinidin 3-glucoside. Also present were 3-glucosides of petunidin and malvidin and glycosides of cyanidin and delphinidin. Seed exudate from …
Detection Of Functional Nicotinic Receptors Blocked By Α-Bungarotoxin On Pc12 Cells And Dependence Of Their Expression On Post-Translational Events, Edward M. Blumenthal, William G. Conroy, Suzanne J. Romano, Paul D. Kassner, Darwin K. Berg
Detection Of Functional Nicotinic Receptors Blocked By Α-Bungarotoxin On Pc12 Cells And Dependence Of Their Expression On Post-Translational Events, Edward M. Blumenthal, William G. Conroy, Suzanne J. Romano, Paul D. Kassner, Darwin K. Berg
Biological Sciences Faculty Research and Publications
A major class of nicotinic receptors in the nervous system is one that binds α-bungarotoxin and contains the α7 gene product. PC12 cells, frequently used to study nicotinic receptors, express the α7 gene and have binding sites for the toxin, but previous attempts to elicit currents from the putative receptors have failed. Using whole-cell patch-clamp recording techniques and rapid application of agonist, we find a rapidly desensitizing acetylcholine-induced current in the cells that can be blocked by α-bungarotoxin. The current amplitude varies dramatically among three populations of PC12 cells but correlates well with the number of toxin-binding receptors. In contrast, …
Effect Of Intermittent Weight Bearing On Soleus Fiber Force-Velocity-Power And Force-Pca Relationships, J. J. Bangart, Jeffrey J. Widrick, Robert H. Fitts
Effect Of Intermittent Weight Bearing On Soleus Fiber Force-Velocity-Power And Force-Pca Relationships, J. J. Bangart, Jeffrey J. Widrick, Robert H. Fitts
Biological Sciences Faculty Research and Publications
Rat permeabilized type I soleus fibers displayed a 33% reduction in peak power output and a 36% increase in the free Ca2+ concentration required for one-half maximal activation after 14 days of hindlimb non-weight bearing (NWB). We examined the effectiveness of intermittent weight bearing (IWB; consisting of four 10-min periods of weight bearing/day) as a countermeasure to these functional changes. At peak power output, type I fibers from NWB animals produced 54% less force and shortened at a 56% greater velocity than did type I fibers from control weight-bearing animals while type I fibers from the IWB rats produced …
Myosin Isoform Heterogeneity In Single Smooth Muscle Cells, Thomas J. Eddinger, Daniel P. Meer
Myosin Isoform Heterogeneity In Single Smooth Muscle Cells, Thomas J. Eddinger, Daniel P. Meer
Biological Sciences Faculty Research and Publications
We review the current understanding of the myosin heavy chain (MHC) isoforms and show that the mRNA levels of smooth muscle (SM)1 and SM2 mimic the expressed levels of SM1 and SM2 protein. The reverse transcriptase-polymerase chain reaction technique has been shown to be sufficiently sensitive to examine SM-MHC expression at the single cell level. Most single smooth muscle cells isolated from adult rabbit carotid express both SM1 and SM2. However, expression of these SM-MHC isoforms at the cellular level is nonuniform and highly variable. This work provides a foundation for future investigations as to the possible unique functional characteristics …
Peak Force And Maximal Shortening Velocity Of Soleus Fibers After Non-Weight-Bearing And Resistance Exercise, Jeffrey J. Widrick, Robert H. Fitts
Peak Force And Maximal Shortening Velocity Of Soleus Fibers After Non-Weight-Bearing And Resistance Exercise, Jeffrey J. Widrick, Robert H. Fitts
Biological Sciences Faculty Research and Publications
Widrick, Jeffrey J., and Robert H. Fitts. Peak force and maximal shortening velocity of soleus fibers after non-weight-bearing and resistance exercise. J. Appl. Physiol. 82(1): 189–195, 1997.—This study examined the effectiveness of resistance exercise as a countermeasure to non-weight-bearing-induced alterations in the absolute peak force, normalized peak force (force/fiber cross-sectional area), peak stiffness, and maximal shortening velocity (V o) of single permeabilized type I soleus muscle fibers. Adult rats were subjected to one of the following treatments: normal weight bearing (WB), non-weight bearing (NWB), or NWB with exercise treatments (NWB+Ex). The hindlimbs of the NWB and NWB+Ex rats were …