Life Sciences Commons^™

The Evolution Of Diet Breadth In Melissodes Bees (Apidae: Eucerini), Karen W. Wright

Biology ETDs

The relationship between phytophagous insects and their host plants has interested scientists since Darwinian times. Using modern phylogenetic inference, we are able to investigate these patterns using, not only the phylogenies of the insects, but the evolutionary relationships among the plants they feed on as well. The relationships between bees and the plants they pollinate were traditionally seen as mutualistic and were treated separately from the research investigating the antagonistic relationships between phytophagous insects and their host plants. However, recent phylogenetic studies have made great progress including bee-host relationships in with the larger body of work on phytophagous insects.

The …

Shedding Light On The Role Of Opaque1 In Asymmetric Cell Division In Maize, Janette Y. Mendoza

Biology ETDs

Asymmetric cell division (ACD) is important for cell fate and tissue patterning. Many aspects of ACD in plants, however, are still unclear. The process of ACD can be broadly broken down into three phases: (1) cell polarization, (2) division plane establishment and maintenance, and (3) cell division and cytokinesis. Stomatal development in maize has proven to be a useful model for understanding the ACD mechanism. Previous studies have identified several proteins important for all three phases of ACD. Actin is important in each of the phases of ACD, suggesting an actin motor such as myosin may also be important for …

Physella Acuta, Comparative Immunology And Evolutionary Aspects Of Gastropod Immune Function, Jonathan H. Schultz

Biology ETDs

Gastropod immunobiology has benefitted from investigations focused on the planorbid snail Biomphalaria glabrata, intermediate host for the human parasite Schistosoma mansoni. Though such concentrated efforts have elucidated fascinating aspects of invertebrate immunity, they have not provided full knowledge regarding the evolution of immune function among other gastropod species. This dissertation presents the importance of making strategic choices regarding which organisms to select for comparative immunology. Herein, the choice was made to investigate the immunobiology of Physella acuta, a freshwater snail species of the Physidae, a sister family to Planorbidae to which B. glabrata belongs. Benefiting greatly from …

Towards A Better Understanding Of The Effects Of Praziquantel On The Interaction Between The Helminth Parasite Schistosoma Mansoni And Its Murine Host, Melissa C. Sanchez

Biology ETDs

Schistosomiasis is a neglected tropical disease (NTD) that infects 206 million individuals worldwide. This disease is caused by dioecious trematodes of the genus Schistosoma, and its pathology is associated with the large number of eggs that are released by the female. Entrapped eggs lead to host immune and inflammatory responses resulting in disease progression. Chemotherapy provides the main means of control and praziquantel (PZQ) is the only widely available drug that is used in all mass drug administration (MDA) programs. As the number of individuals receiving PZQ continues to grow, the development of PZQ resistance is a concern and …

Avian Thermoregulation In The Heat: Resting Metabolism, Evaporative Cooling And Limits To Activity In Sonoran Desert Birds, Eric Smith

Biology ETDs

Birds in subtropical deserts face significant thermoregulatory challenges as environmental temperatures regularly exceed avian body temperature. To understand the differing susceptibility of desert birds to increasing temperatures, this dissertation initially examined thermoregulatory performance in seven passerine bird species varying in body mass from 10 to 70g – Lesser Goldfinch, House Finch, Pyrrhuloxia, Cactus Wren, Northern Cardinal, Abert’s Towhee and Curve-billed Thrasher – and three larger Sonoran Desert nesting bird species – Mourning Dove (104 g), White-winged Dove (147 g) and Gambel’s Quail (161 g). Daytime resting metabolism, evaporative water loss and real-time body temperature were measured using flow-through respirometry at …

How Does Antibiotic Resistance Spread In Tuberculosis?, Julie A. Spencer

Shared Knowledge Conference

The ancient bacterial disease of tuberculosis (TB) is curable with antibiotics, but according to the World Health Organization, in 2016 over 10 million people became infected with the disease. 600,000 of these cases were resistant to antibiotics, yet the worldwide treatment success rate for drug resistant TB is only 54%. Furthermore, strains exist now that are resistant to all known antibiotics. In the current environment of global travel, this poses a risk for emergent epidemics of drug resistant TB. In this study, I asked: after an antibiotic resistant strain has evolved in someone’s lungs, how does it spread? It is …

Engage A Voice, Repress Fatigue; The Coincident Evolution Of Hominin Vocalization And A Metabolic Threshold, Galen A. Morton 9935238, Martin L. Morton

Shared Knowledge Conference

Physiologically self-protective mechanisms borne from hominin evolutionary history that increase survivability are not unknown to science. In reviewing exercise science literature regarding testing and assessment measures of subjects talking while exercising, a logical question has materialized: To what degree has evolution facilitated synchronization of comfortable oral communication with sustainable exercise intensity? An individual able to engage a voice, represses fatigue. The Talk Test, is a practical strategy whereby a subject deliberately speaks during an exercise protocol. It is a common tool in both kinesiology and clinical fields because it inherently identifies a pivotal metabolic threshold. The coincidence of comfortable ability …

Mechanisms For The Spread Of Antibiotic Resistance In Tuberculosis, Julie Allison Spencer

Shared Knowledge Conference

Tuberculosis (TB) is currently the ninth leading cause of death for humans worldwide, causing 10.4 million new infections in 2016. According to the World Health Organization, of these infections, 600,000 were antibiotic resistant. However, the treatment success rate for resistant TB was only 54%. The potential for emergent epidemics of drug resistant TB highlights the need to understand the mechanisms for the spread of resistance. Mycobacterium tuberculosis, the bacterium that causes TB, evolves resistant strains within individuals who are being treated with antibiotics. It has been assumed previously that the resistant characteristics of these strains are spread primarily by the …

Intraspecific Floral Color Variation As Perceived By Pollinators And Non-Pollinators: Evidence For Pollinator-Imposed Constraints?, Kellen Cedar Paine

Biology ETDs

Pollinator-mediated selection is expected to constrain floral color variation within plant populations, yet populations with high color variability are common in nature. To explore this, we collected floral reflectance spectra for 34 populations of 14 plant species of New Mexico, USA, and translated them into three different visual spaces. We found evidence that the majority comparisons were indistinguishable to bees, the dominant pollinator group. We also found that floral color variation was significantly greater for two non-pollinating groups, birds and humans. Our results suggest that a portion of human-perceived floral color variation within populations persists because it is invisible to …

2017 Annual Report, Christopher C. Witt

Annual Reports

No abstract provided.

Hemocyanin-Like 1 Protein, Unique To Planorbid Snails, Supports Reproductive Output Of Biomphalaria Glabrata, Vector Of Schistosomiasis, Janeth Pena

Biology ETDs

The Neotropical freshwater snail, Biomphalaria glabrata is a major intermediate host of the parasite Schistosoma mansoni, causative agent of human intestinal schistosomiasis. Disease control efforts consist mainly of mass drug treatment but it is recognized that targeting of the snail vector is essential to prevent parasite transmission that leads to rapid (re)infections. This dissertation combines the knowledge of basic snail biology with modern molecular techniques including protein analysis, next-generation sequencing, proteomics, qRT-PCR and RNA interference to: (1) characterize hemocyanin-like genes in B. glabrata, in particular with further description of the egg mass fluid (EMF) protein hemocyanin-like 1 (Hcl-1), (2) …

The Fate Of Xylem-Transported Co2 In Plants, Samantha S. Stutz, David T. Hanson

Biology ETDs

The concentration of carbon dioxide in tree stems can be ~30-750 times higher than current atmospheric [CO₂]. Dissolved inorganic carbon enters the xylem from root and stem respiration and travels with water through the plant. However, the fate of much of this xylem-transported CO₂ is unknown. In these studies I examined the fate of xylem-transported CO₂ traveling through the petiole and leaf. This was accomplished by placing cut leaves from a woody and herbaceous C₃ species, and a Kranz-type C₄ species, in a solution of dissolved NaH¹³CO₃ at concentrations similar to …

From Olfaction To Immunity: Characterization Of Nasal Immunity In Bony Fish, Ali Sepahi

Biology ETDs

The olfactory system is a common route pathogen entry in vertebrates. As a consequence, the nasopharynx-associated lymphoid tissue (NALT) needs to rapidly clear infections without compromising the sense of olfaction. NALT is present in teleost fish but its cellular and molecular mechanisms of action have not been investigated to this date. This dissertation focuses on three aims: 1. investigating the role of CCL19-like as a primordial chemokine in vertebrate nasal immunity, 2. determining the presence of tissue microenvironments within the olfactory organ (OO) of rainbow trout, and 3. understanding the immune contributions of olfactory sensory neurons (OSNs) in teleosts against …

Genomic Signatures Of Adaptive Evolution, Jessica Weber

Biology ETDs

Comparative genomics has revolutionized virtually all fields of biology including the study of evolution. In this dissertation, I used next-generation sequencing to explore the evolutionary histories and adaptive evolution of a diverse set of taxa. Comparisons ranged across time scales, from population-level genetic diversity studies to questions spanning the deepest branches of the metazoan lineage. Whole genome sequencing of 50 unrelated Korean individuals revealed that Koreans have a distinct genetic history from the Chinese and Japanese populations. Our Korean-specific variome database was used to identify novel disease-causing variants in the Korean population, highlighting the value of high-quality ethnic variation databases …

Investigations On The Role Of The Immune System In Mammary Development And Maternal Immunity In The Marsupial, Monodelphis Domestica, Bethaney Fehrenkamp

Biology ETDs

All newborn mammals are highly dependent upon milk for nourishment and immune protection. This is especially true for marsupials, a lineage of mammals with a short gestation, limited placental development, and an increased reliance on an extended lactation period. Most newborn marsupials do not receive passive maternal immunity in utero and therefore are entirely dependent upon factors within the milk for immune protection until capable of mounting their own response. In this project we seek to characterize the complex lactation program utilized by marsupials, and seek greater understanding of the maternal role in the establishment of the developing immune system …

Soil Properties Explain Changes In Soil Microbial Enzyme Activity With Depth In A Piñon-Juniper Woodland, Amanda B. Sacks

Biology ETDs

Semi-arid ecosystems play a major role in the global carbon cycle. These ecosystems, including piñon-juniper (PJ) woodlands, are experiencing extreme drought. As such, it is vital to characterize both above and below-ground processes in these systems in order to understand their vulnerability to future drought and other changes in climate.

Soils microbial communities play a critical role in nutrient cycles, as well as carbon storage, within PJ woodlands. More specifically, microbes aid the decomposition and mineralization of key nutrients, including carbon, nitrogen and phosphorus, through the use of extracellular enzymes. Measuring microbial enzyme activity, therefore, can provide insight into how …

Systematics And Diversification Of The Pantropical Avian Order Coraciiformes, Jenna Merle Mccullough

Biology ETDs

How and why species diversify is a central theme of evolutionary biology. Species-rich, morphologically diverse, pantropical clades provide rare opportunities to explore questions about drivers of diversification in the tropics. Here, we present the first complete species tree of Coraciiformes (6 families, ~177 species of kingfishers, motmots, bee-eaters, and allies), produced with thousands of ultraconserved elements. We recovered a well-supported tree which shows that there are two clades within Coraciiformes: 1) bee-eaters sister to rollers + ground- rollers and 2) todies sister to motmots + kingfishers. We estimated the biogeographical history of the group, explored bill shape evolution with a …

Rodent Responses To Drought In The Southwestern United States: Resource Utilization Strategies And Effects To Keystone Resources, Charles L. Hayes Iv

Biology ETDs

The role of abiotic and biotic factors in regulating community and population dynamics is a central question of ecological inquiry. In the southwestern United States, the North American monsoon supplies vital pulses of moisture to Sonoran desert, arid grassland, and montane communities. I evaluated abiotic limitation in prairie and montane populations of Gunnison’s prairie dog (Cynomys gunnisoni) using stable isotope analysis to quantify foraging niche widths. Niche widths declined under periods of drought stress. Prairie dogs in montane habitats exhibited seasonal shifts in dietary niche width during favorable growth periods for more nutritious plants using the C_{3 …}

Schistosomiasis In The Wild: A Transcriptomics Perspective On Field-Derived Biomphalaria Pfeifferi And Schistosoma Mansoni, And Their Interactions, Sarah K. Buddenborg

Biology ETDs

Schistosomiasis, caused by trematodes in the genus Schistosoma, is a widespread neglected tropical disease with the species S. mansoni infecting over 100 million people. We aimed to better understand the snail host and parasite responses during intramolluscan stages of infection by performing dual RNA-Seq on field-collected snails with natural infections from western Kenya. We collected uninfected Biomphalaria pfeifferi, B. pfeifferi with a patent cercariae-producing S. mansoni infection, and B. pfeifferi exposed to field-collected S. mansoni at 1 and 3d (days post infection).

We first created a high-quality B. pfeifferi transcriptome to identify the snail response to S. mansoni infection. …

Linking Climate Change And Mortality In Piñon-Juniper Woodlands, From Leaf To Ecosystem, Amanda I. Liebrecht

Biology ETDs

As global climate changes, the Southwestern US is predicted to experience more frequent and intense drought events. Extreme droughts can drive decreases in both physiological and ecosystem function, and can result in widespread tree mortality. Piñon-juniper (PJ) woodlands are a prevalent ecosystem in the region, co-dominated by two tree species, piñon (Pinus edulis) and juniper (Juniperus monosperma). Drought-induced piñon mortality has occurred over the past few decades, coinciding with outbreaks of a piñon-specific bark beetle. Piñon and juniper have different hydraulic strategies (isohydry and anisohydry, respectively) that should affect the way each species responds to drought. …

Understanding Patterns Of Diversity And Evolution In Mainland Anolis Lizards, Levi Gray

Biology ETDs

Patterns of organismal diversity and evolution are often difficult to interpret with a high level of confidence. The number of mechanisms and processes that contribute to shaping patterns of diversity is extensive and is reflected in the many methods researchers have used to infer causation. Taxonomic groups that are well-studied can offer more precise interpretation of pattern and process due to the considerable amount of research addressing ecology, natural history, and behavior of the organisms.

In this dissertation, I explore patterns of phylogenetic and phenotypic variation in Anolis lizards (anoles) by testing hypotheses that could have led to the observed …

Prevalence Of Artemisinin Resistance Associated Single Nucleotide Polymorphisms In Kenyan Isolates, Elizabeth M. Glenn

Biology ETDs

Malaria has had an unspeakable toll on human economy and wellbeing. Every year, there are approximately 212 million malaria infections caused by Plasmodium falciparum leading to 429,000 deaths, the vast majority of which occur in sub-Saharan Africa where the parasite is endemic. Decades of widespread use, and probable misuse, of antimalarial drugs has created extraordinary selective pressure on the parasite and made many formerly effective drugs ineffective. Currently, artemisinin and artemisinin derivatives are the most effective treatment available but resistance to dihydroartemisinin (DHA), the active ingredient, is emerging. Continued monitoring for genetic mutations linked with resistance is vital to global …

Seasonal Shifts In C3 And C4 Resource Use By A Small Mammal Community Under Changing Precipitation Regimes, Alaina D. Pershall

Biology ETDs

In light of climate change and projections of increasing temperatures and aridity in the North American southwest, it is essential to understand how consumer populations will respond to changes in the resource landscape. Rainfall varies in timing and intensity and therefore the timing, proportion, phenology, and abundance of C₃ and C₄ plant resources vary seasonally and annually. Here we examine rodent resource use in the Chihuahuan desert and focus on two distinct precipitation pulses in this system, where spring C₃ plants increase production in response to winter rains and C₄ plants respond to summer monsoons. We …

Using D13c, D15n, And D2h To Better Understand The Ecology Of Green Sea Turtles, Laura Pagès Barceló

Biology ETDs

Many green sea turtle populations are slowly recuperating from a recent severe decline due to anthropogenic factors including human consumption and mortality related to the fishing industry. Despite being charismatic animals that have been extensively studied, there is still a limited understanding of their feeding strategies and diet plasticity. This research explores the use of hydrogen isotopes in marine ecosystems to better understand green sea turtle ecology. This study is presented in two chapters: I first examined the trophic discrimination factor (D²H_NET) for hydrogen isotope (d²H) as a tool to correct hydrogen isotope data …

2018 Annual Report, Christopher C. Witt

Annual Reports

In 2018, the Museum of Southwestern Biology (MSB) has continued to improve its profile and impacts, both on the University of New Mexico campus and in the international scientific community. Its collections serve as scientific infrastructure that enhances research, teaching, community service, and public outreach. The MSB is part of the UNM Department of Biology, and the missions of the MSB and the Department are synergistic. MSB houses extensive and rapidly growing collections representing biodiversity of world, primarily from the last halfcentury. MSB has outstanding collections from New Mexico and western North America, but it also has substantial holdings from …