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Full-Text Articles in Life Sciences
Combination Of Classical And Statistical Approaches To Enhance The Fermentation Conditions And Increase The Yield Of Lipopeptide(S) By Pseudomonas Sp. Oxdc12: Its Partial Purification And Determining Antifungal Property, Vivek Chauhan, Vivek Dhiman, Shamsher S. Kanwar
Combination Of Classical And Statistical Approaches To Enhance The Fermentation Conditions And Increase The Yield Of Lipopeptide(S) By Pseudomonas Sp. Oxdc12: Its Partial Purification And Determining Antifungal Property, Vivek Chauhan, Vivek Dhiman, Shamsher S. Kanwar
Turkish Journal of Biology
Around 200 different lipopeptides (LPs) have been identified to date, most of which are produced via Bacillus and Pseudomonas species. The clinical nature of the lipopeptide (LP) has led to a big surge in its research. They show antimicrobial and antitumor activities due to which mass-scale production and purification of LPs are beneficial. Response surface methodology (RSM) approach has emerged as an alternative in the field of computational biology for optimizing the reaction parameters using statistical models. In the present study, Pseudomonas sp. strain OXDC12 was used for production and partial purification of LPs using Thin Layer Chromatography (TLC). The …
Evaluation Of Recombinant Human Vascular Endothelial Growth Factor Vegf121-Loaded Poly-L-Lactide Microparticles As A Controlled Release Delivery System, Sunil Abraham, Srinivasa Prasad Rangaswamy, Amutha Chinnaiah
Evaluation Of Recombinant Human Vascular Endothelial Growth Factor Vegf121-Loaded Poly-L-Lactide Microparticles As A Controlled Release Delivery System, Sunil Abraham, Srinivasa Prasad Rangaswamy, Amutha Chinnaiah
Turkish Journal of Biology
Vascular endothelial growth factor A (VEGF-A) is an important growth factor that plays a major role in angiogenesis. With different isoforms distributed in various tissues, the shortest isoform of VEGF-A is VEGF121, one of the physiologically functional variants next to VEGF165. It is well known that VEGF has a shorter half-life, and the stability of the protein must be considered in therapeutic aspects. Poly-l-lactide (PLA) microparticles can release the encapsulated protein in a sustained release mode. In this study, the VEGF121 gene was cloned and expressed in a prokaryotic expression system (Escherichia coli). The recombinant VEGF121 was encapsulated with PLA …
Heterologous Expression And Characterization Of A High Redox Potential Laccase Fromcoriolopsis Polyzona Mucl 38443, Orkun Pi̇nar, Candan Tamerler Behar, Ayten Karataş
Heterologous Expression And Characterization Of A High Redox Potential Laccase Fromcoriolopsis Polyzona Mucl 38443, Orkun Pi̇nar, Candan Tamerler Behar, Ayten Karataş
Turkish Journal of Biology
In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 …
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Turkish Journal of Biology
No abstract provided.
Heterologous Expression And Purification Of Porcine Fat Mass Andobesity-Associated Gene In Escherichia Coli, Bo Zhou, Xiaoling Chen, Zhiqing Huang, Gang Jia, Guangmang Liu
Heterologous Expression And Purification Of Porcine Fat Mass Andobesity-Associated Gene In Escherichia Coli, Bo Zhou, Xiaoling Chen, Zhiqing Huang, Gang Jia, Guangmang Liu
Turkish Journal of Biology
A porcine fat mass and obesity-associated gene (pFTO) was cloned into the expression vector pET30a(+) and heterologously expressed in Escherichia coli. The effects of isopropyl ß-D-thiogalactopyranoside (IPTG) concentration and induction time on the expression of recombinant pFTO were assessed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the molecular mass of the recombinant pFTO protein was about 56 kDa, and the recombinant protein could be induced efficiently in E. coli BL21 by the addition of 0.75 mM IPTG for 4 h at 30 °C. The recombinant pFTO fusion protein was purified using Ni-IDA affinity chromatography with a yield of about 1.8 …
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Turkish Journal of Biology
An intracellular nitrilase (Nit1) with cyanide-degrading activity was isolated from Trichoderma harzianum VSL291, cultivated on benzonitrile as the sole carbon source. Nit1 was purified to homogeneity by ion exchange and gel filtration chromatography with a recovery of 7.15% and a fold of 22.5. The molecular weight was estimated to be 47.7 kDa and the purified enzyme was sequenced with a system of liquid chromatography and mass spectrometry (LC-MS). The enzyme consists of 436 amino acids with a predicted molecular weight of 47.088 kDa. The sequence revealed conserved domains for a nitrilase super family such as putative active and binding sites …
Prokaryotic Expression, Purification, Polyclonal Antibody Preparation, And Tissue Distribution Of Porcine Six1, Meng Xu, Xiaoling Chen, Zhiqing Huang, Wanxue Wen, Shuai Chang, Xiaoyan Wang, Daiwen Chen, Bing Yu, Junqiu Luo, Guangmang Liu
Prokaryotic Expression, Purification, Polyclonal Antibody Preparation, And Tissue Distribution Of Porcine Six1, Meng Xu, Xiaoling Chen, Zhiqing Huang, Wanxue Wen, Shuai Chang, Xiaoyan Wang, Daiwen Chen, Bing Yu, Junqiu Luo, Guangmang Liu
Turkish Journal of Biology
Sine oculis homeobox 1 (Six1), a member of the Six homeoproteins, plays an important role in skeletal myogenesis and the specification of myofiber diversity. In this study, in order to scale up the production of recombinant porcine Six1 (pSix1), a pET-30a(+)-pSix1 plasmid was constructed and transformed into Escherichia coli BL21 (DE3). The recombinant pSix1 could be induced for efficient expression with 2 mM IPTG for 2 h at 30 °C, yielding approximately 4.6 mg/L. The protein was then purified and identified by western blot, and it was used for preparing its polyclonal antibody. The recombinant pSix1 was tagged with a …
Optimization And Characterization Of Cold-Active Endoglucanase Produced By Aspergillus Terreus Strain Akm-F3 Grown On Sugarcane Bagasse, Abhas Kumar Maharana, Pratima Ray
Optimization And Characterization Of Cold-Active Endoglucanase Produced By Aspergillus Terreus Strain Akm-F3 Grown On Sugarcane Bagasse, Abhas Kumar Maharana, Pratima Ray
Turkish Journal of Biology
The psychrotolerant microfungus Aspergillus terreus strain AKM-F3 (GenBank Accession No. KF417464) was investigated for the production of cold-active endoglucanase using sugarcane bagasse. The effects of different process parameters were studied for hyperproduction. Optimum production was found at 15 °C with an initial pH of 5.0 at 120 h. For enzyme production, 1 mM ZnCl_2, 0.01% aspartate, 2% lactose, and 3% NaNO_3 were regarded as potent inducers. The final production gave an activity 3.764-fold higher than the unoptimized conditions. The molecular weight of purified endoglucanase was 55 kDa, showing a 3.464-fold purification increase with a 23.925% yield having 75.279 U/mg of …
An Extracellular Antifungal Chitinase From Lecanicillium Lecanii: Purification, Properties, And Application In Biocontrol Against Plant Pathogenic Fungi, Huu Quan Nguyen, Dinh Thi Quyen, Sy Le Thanh Nguyen, Van Hanh Vu
An Extracellular Antifungal Chitinase From Lecanicillium Lecanii: Purification, Properties, And Application In Biocontrol Against Plant Pathogenic Fungi, Huu Quan Nguyen, Dinh Thi Quyen, Sy Le Thanh Nguyen, Van Hanh Vu
Turkish Journal of Biology
An extracellular antifungal chitinase from L. lecanii strain 43H was purified by ammonium sulfate precipitation and DEAE-Sephadex A-50 ion exchange chromatography; it showed a molecular mass of approximately 33 kDa with a specific activity of 167.5 U/mg protein and purification factor of 2.5. Optimum temperature and pH were observed at 40 °C and pH 6.0, respectively. This enzyme was stable at up to 40 °C and at pH 5.0-6.0. The kinetic constants K_m and V_{max} determined for the chitinase with colloidal chitin as substrate was 0.82 mg/mL and 4.51 U/mg, respectively. The presence of 5-15 mM tested metal ions led …
Expression, Purification, And Characterization Of Recombinant Humanparaoxonase 1 (Rhpon1) In Pichia Pastoris, Yağmur Ünver, Esabi̇ Başaran Kurbanoğlu, Orhan Erdoğan
Expression, Purification, And Characterization Of Recombinant Humanparaoxonase 1 (Rhpon1) In Pichia Pastoris, Yağmur Ünver, Esabi̇ Başaran Kurbanoğlu, Orhan Erdoğan
Turkish Journal of Biology
The main purpose of the present study was to perform the expression in Pichia pastoris X-33 of the human paraoxonase 1 (hPON1) enzyme, which is a mammalian serum protein. Extracellular hPON1 enzyme was expressed with the pPICZ?A vector using a strong AOX promoter, and enzyme secretion in the fermentation medium was achieved by means of Saccharomyces cerevisiae alpha factor signal sequence. The recombinant cells were grown in a shaking flask containing production medium. SDS-PAGE and Western blot analysis illustrated that the molecular mass of extracellular hPON1 enzyme produced by the recombinant P. pastoris strain was 59.1 kDa. Biochemical characterization of …
Purification And Characterization Of A Novel Detergent- And Organic Solvent-Resistant Endo-Beta-1,4-Glucanase From A Newly Isolated Basidiomycete Peniophora Sp. Ndvn01, Dinh Kha Trinh, Dinh Thi Quyen, Thi Tuyen Do, Ngoc Minh Nghiem
Purification And Characterization Of A Novel Detergent- And Organic Solvent-Resistant Endo-Beta-1,4-Glucanase From A Newly Isolated Basidiomycete Peniophora Sp. Ndvn01, Dinh Kha Trinh, Dinh Thi Quyen, Thi Tuyen Do, Ngoc Minh Nghiem
Turkish Journal of Biology
A novel extracellular endoglucanase from a basidiomycete strain Peniophora sp. NDVN01 was purified 2.8-fold to homogeneity through ammonium sulfate precipitation and gel filtration with Bio-Gel P-100 and Sephadex G-75. The endoglucanase had a specific activity of 163.8 U/mg protein and a molecular mass of 32 kDa. Optimum temperature and pH were at 60 °C and 4.5, respectively. The enzyme was stable at up to 42 °C and in the pH range of 3.5-5.5 with a residual activity of over 80% for 24 h of treatment. Ni^2^+ activated but other metal ions showed no or slight inhibitory effect on the enzyme …
Purification And Characterization Of Trehalase From Seeds Of Chickpea (Cicer Arietinum L.), Maimona Kord, Elhusseiny Youssef, Hanaa Ahmed, Ebtesam Qaid
Purification And Characterization Of Trehalase From Seeds Of Chickpea (Cicer Arietinum L.), Maimona Kord, Elhusseiny Youssef, Hanaa Ahmed, Ebtesam Qaid
Turkish Journal of Biology
In the present study, trehalase was purified and characterized from the seeds of Cicer arietinum L. Giza 1. Crude extract was prepared and purified for electrophoretic homogeneity using ammonium sulfate, chromatography on DEAE-cellulose, CM Sepharose, and Sephadex G-200. The final specific activity was 7 U/mg protein, with 232-fold purification. The purified enzyme exhibited its pH optimum at 5.5. The optimum temperature was 60 °C. The determined K_m value was 3.64 mM trehalose. The enzyme activity was stimulated by 20 mM Mn^{2+}, Ni^{2+}, or Co^{2+}, while it was inhibited by 20 mM Na^+, K^+, Li^+, Ca^{2+}, Zn^{2+}, Cu^{2+}, or Fe^{3+}. Zn^{2+} …
Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova
Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova
Turkish Journal of Biology
An extracellular multiple form of endoxylanase was isolated from the xylanolytic complex of Aspergillus niger B03. The enzyme was purified to a homogenous form using ultrafiltration, anion exchange chromatography, and gel filtration. It was a nonglycosylated protein with a molecular weight of 20,000 Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 21,000 Da as determined by gel filtration. The optimal pH for the enzyme action was 5.0 and the optimal temperature was 55 °C. Endoxylanase stability was significantly improved in the presence of glycerol and sorbitol. The enzyme activity was activated by Mn^{2+} and Co^{2+}, and it was …
An Anti-Shigella Dysenteriae Bacteriocin From Pediococcus Pentosaceus Mtcc 5151 Cheese Isolate, Renu Agrawal, Shylaja Dharmesh
An Anti-Shigella Dysenteriae Bacteriocin From Pediococcus Pentosaceus Mtcc 5151 Cheese Isolate, Renu Agrawal, Shylaja Dharmesh
Turkish Journal of Biology
A cheese isolate Pediococcus pentosaceus lactic acid bacterium, which has been deposited at the Microbial Type Culture Collection Centre Chandigarh with the accession number MTCC 5151, was tested for anti-Shigella dysenteriae activity and the bacteriocin was characterized. The protein band was observed with tricine sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass of 23 kDa. This is a new and novel bacteriocin that inhibits S. dysenteriae and has not yet been reported from P. pentosaceus. It was purified on a Sephacryl column and the active fraction specific for anti-Shigella dysenteriae with 23 kDa …
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Turkish Journal of Biology
An extracellular endoglucanase (EG) from Aspergillus niger VTCC-F021 was purified 2.09-fold to homogeneity with a yield of 18.4%. The enzyme had a molecular mass of 31 kDa and a specific activity of 14.122 U/mg protein. Optimum temperature was observed at 55 °C and optimum pH at 5. The enzyme was stable up to 50 °C and from pH 5 to 6 with residual activity >80% and 60%, respectively. The kinetic constants K_m and V_{max} determined for EG, with carboxyl methyl cellulose as a substrate, were 8.5815 mg CMC/mL and 20.121 U/mg protein, respectively. EDTA increased EG activity by 35% at …
Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan
Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan
Turkish Journal of Biology
The effects of various carbon and nitrogen sources on production of \alpha-amylase by Bacillus subtilis and its mutant derivates were investigated. The maximum production of \alpha-amylase by all strains was obtained in the presence of mesoinositol as the carbon source. There was no more significant increase in enzyme yield in the case of the supplementation of nitrogen sources, whereas malt extract and tryptone were preferred nitrogen sources for amylase production by Bacillus subtilis and mutant U 2-6 strain, respectively. \alpha-Amylases of B. subtilis and its mutant strain (EBUE 5-3) were purified through a series of steps, and characterized. The optimum …
Partial Purification Of Intestinal Triglyceride Lipase From Cyprinion Macrostomus Heckel, 1843 And Effect Of Ph On Enzyme Activity, Naci̇ Değerli̇, M. Ali̇ Akpinar
Partial Purification Of Intestinal Triglyceride Lipase From Cyprinion Macrostomus Heckel, 1843 And Effect Of Ph On Enzyme Activity, Naci̇ Değerli̇, M. Ali̇ Akpinar
Turkish Journal of Biology
Intestinal triglyceride lipase (TG) of Cyprinion macrostomus after precipitation with 30% polyethylene glycol (PEG), was partially purified by hydrophobic interaction chromatography on phenyl sepharose CL-4B, and the effect of pH on this enzyme activity was determined titrimetrically. Intestinal tissue homogenates were precipitated using 30% PEG-8000, and then applied onto a phenyl sepharose CL-4B column for hy-drophobic interaction chromatography. The lipase that bound to this hydrophobic resin and was partially purified by this single step application was then eluted from this resin with taurocholic acid and Triton X-100 elution. This purified enzyme effectively hydrolysed natural olive oil substrate under the experimental …
Purification And Determination Of The Molecular Structure Of Hemolymph Lectin Of Agrotis Segetum (Denis And Schiff.), Nursel Gül, Cevat Ayvali
Purification And Determination Of The Molecular Structure Of Hemolymph Lectin Of Agrotis Segetum (Denis And Schiff.), Nursel Gül, Cevat Ayvali
Turkish Journal of Biology
A soluble lectin was purified from the hemolymph of Agrotis segetum by two methods, via affinity chromatography on a Sepharose-4B column and gel filtration on a Superdex-200 column. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed a single protein band with a molecular weight of 69,000 in both the presence and absence of 2-Mercaptoethanol. Alternatively, the molecular weight of this lectin was estimated to be 69,000 by gel filtration on Superdex-200. Electron microscopic observation of the purified lectin was reticular in structure and consisted of tandem aligned spherical basic units.
Purification Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Meti̇n Yildirim
Purification Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Meti̇n Yildirim
Turkish Journal of Biology
Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a rapid and simple three-step purification procedure including freeze drying, silicic acid adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa by tricine SDS-PAGE.
Anthranilate Synthetase From Escherichia Coli Sjhi:Purification And Some Properties, Münevver Arisoy
Anthranilate Synthetase From Escherichia Coli Sjhi:Purification And Some Properties, Münevver Arisoy
Turkish Journal of Biology
A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially using concentrations greater than 10%. A positive effect 2-Mercapto-ethanol on the enzyme stability was also determined.