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Microfluidics-Integrated Time-Lapse Imaging For Analysis Of Cellular Dynamics, Dirk Albrecht, Gregory Underhill, Joshua Resnikoff, Avital Mendelson, Sangeeta Bhatia, Jagesh Shah

Dirk R. Albrecht

An understanding of the mechanisms regulating cellular responses has recently been augmented by innovations enabling the observation of phenotypes at high spatio-temporal resolution. Technologies such as microfluidics have sought to expand the throughput of these methods, although assimilation with advanced imaging strategies has been limited. Here, we describe the pairing of high resolution time-lapse imaging with microfluidic multiplexing for the analysis of cellular dynamics, utilizing a design selected for facile fabrication and operation, and integration with microscopy instrumentation. This modular, medium-throughput platform enables the long-term imaging of living cells at high numerical aperture (via oil immersion) by using a conserved …