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Immobilization Nematodes For Live Imaging Using An Agarose Pad Produced With A Vinyl Record, Katherine A. Rivera Gomez, Mara Schvarzstein
Immobilization Nematodes For Live Imaging Using An Agarose Pad Produced With A Vinyl Record, Katherine A. Rivera Gomez, Mara Schvarzstein
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Numerous microfluidic systems have been developed and used for live imaging of Caenorhabditis nematodes (Allen et al., 2008; Zhang et al., 2008; Krajniak and Lu, 2010; Krajniak et al., 2013; Cornaglia et al., 2015). These systems can be costly, complex to set up, or require high-maintenance between uses. In addition, microfluidic rigs can be thick, preventing live imaging of worms from strains expressing low fluorescence fusion proteins. In the absence of elaborate microfluidic rigs, most live imaging protocols utilize flat agarose pads along with anesthetics and/or microbeads to immobilize the nematodes (Kim et al., 2013). Since this method does not …