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Molecular Cloning Of Lipocalin-2 Into A Eukaryotic Vector And Its Expression Inbovine Mammary Epithelial Cells As A Potential Treatment For Bovine Mastitis, Neelesh Sharma, Simrinder Singh Sodhi, Jeong Hyun Kim, Mrinmoy Ghosh, Jiao Jiao Zhang, Deog Bon Koo, Man Jong Kang, Dong Kee Jeong
Molecular Cloning Of Lipocalin-2 Into A Eukaryotic Vector And Its Expression Inbovine Mammary Epithelial Cells As A Potential Treatment For Bovine Mastitis, Neelesh Sharma, Simrinder Singh Sodhi, Jeong Hyun Kim, Mrinmoy Ghosh, Jiao Jiao Zhang, Deog Bon Koo, Man Jong Kang, Dong Kee Jeong
Turkish Journal of Biology
Lipocalin-2 (LCN2) is a 25-kDa protein in the lipocalin family that shows antibacterial activity. The aim of this study was to clone human LCN2 into the mammalian-specific pIRES2-AcGFP1 vector and to determine the antibacterial activity of the recombinant pIRES2-AcGFP1-hLCN2 plasmid against major bacterial pathogens that cause bovine mastitis. The amplified hLCN2 was successfully cloned into the vector, and the plasmid was transfected into bovine mammary epithelial stem cells using nucleofection. Immunochemistry, reverse transcription polymerase chain reaction, and ELISA were used to monitor and quantify hLCN2 expression in the cells. After 1 day of incubation, the concentration of hLCN2 secreted by …