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The Mtt Viability Assay Yields Strikingly False-Positive Viabilities Although The Cells Are Killed By Some Plant Extracts, Di̇dem Karakaş, Ferda Ari, Engi̇n Ulukaya
The Mtt Viability Assay Yields Strikingly False-Positive Viabilities Although The Cells Are Killed By Some Plant Extracts, Di̇dem Karakaş, Ferda Ari, Engi̇n Ulukaya
Turkish Journal of Biology
The MTT assay is one of the often used cell viability/cytotoxicity assays. However, when the methanol extracts of plants are used to test their cytotoxic potential, interference may occur, resulting in false-positive viability results. Therefore, in this study, the reliability of the MTT assay was investigated in the case of plant use. The methanol extracts of three different plants (Hypericum adenotrichum, Salvia kronenburgii, and Pelargonium quercetorum) were tested in breast cancer cell lines (MCF-7 and MDA-MB-231) using the MTT assay and the results were compared to the ATP assay, which is a much more sensitive and reliable assay due to …