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Articles 1 - 30 of 95
Full-Text Articles in Life Sciences
Tgf-β: Its Role In The Differentiation And Function Of T Regulatory And Effector Cells, Sujuan Yang, Songguo Zheng
Tgf-β: Its Role In The Differentiation And Function Of T Regulatory And Effector Cells, Sujuan Yang, Songguo Zheng
Turkish Journal of Biology
The cytokine transforming growth factor-β (TGF-β) plays an important role in the maintenance of T cell homeostasis, immune tolerance to self-antigens, and T cell differentiation during immune responses. TGF-β has pleiotropic effects on adaptive immune responses, especially on the differentiation of T helper and T regulatory cell populations, which depends on the T cell differentiation status and the stimulation conditions. This review provides an update about the roles of TGF-β in T cell responses, especially in the regulation of Treg development and biological features under steady state and inflammation. We also discuss the additional roles that TGF-β plays in the …
The Application Of Mesenchymal Progenitor Stem Cells For The Reduction Of Oxidative Stress In Animals, Edyta Wojtas, Andrzej Zachwieja, Anna Zwyrzykowska, Robert Kupczynski, Krzysztof Marycz
The Application Of Mesenchymal Progenitor Stem Cells For The Reduction Of Oxidative Stress In Animals, Edyta Wojtas, Andrzej Zachwieja, Anna Zwyrzykowska, Robert Kupczynski, Krzysztof Marycz
Turkish Journal of Biology
Oxidative stress is the primary cause of lowering of the body's immune status. It arises as a result of intense metabolic processes in humans and animals that involve a decrease in antioxidant enzymes activity at the expense of increased production of free radicals and reactive oxygen species (ROS). Consequently, this leads to oxidation of lipids, proteins, DNA, and RNA, causing cell proliferation reduction, faster cell aging, and subsequent degradation. Currently, there is a search for methods to maintain an oxidation-antioxidant balance. The results of many authors' research point to the effectiveness of the use of mesenchymal stem cells (MSCs) in …
Tumor Necrosis Factor-Alpha Induced Caspase-3 Activation-Related Inos Gene Expression In Adp-Activated Platelets, Özge Çevi̇k, Zelal Adigüzel, Ahmet Tarik Baykal, Azi̇ze Şener
Tumor Necrosis Factor-Alpha Induced Caspase-3 Activation-Related Inos Gene Expression In Adp-Activated Platelets, Özge Çevi̇k, Zelal Adigüzel, Ahmet Tarik Baykal, Azi̇ze Şener
Turkish Journal of Biology
Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-α in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-α and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-α and/or 50 mM iNOS …
Expression Of The Zmdef1 Gene And α-Amylase Inhibitory Activityof Recombinant Defensin Against Maize Weevils, Thi Xuan Thuy Vi, Hoang Duc Le, Vu Thanh Thanh Nguyen, Van Son Le, Hoang Mau Chu
Expression Of The Zmdef1 Gene And α-Amylase Inhibitory Activityof Recombinant Defensin Against Maize Weevils, Thi Xuan Thuy Vi, Hoang Duc Le, Vu Thanh Thanh Nguyen, Van Son Le, Hoang Mau Chu
Turkish Journal of Biology
Plant defensins are multifunctional small cysteine-rich proteins. They are active against fungi, bacteria, and many viruses, and they inhibit trypsin and α-amylase activities. In this study, we expressed the maize defensin gene (ZmDEF1) in tobacco seeds in order to establish the basis for generating transgenic maize plants resistant to weevils. The ZmDEF1 gene was isolated from Maison, a Vietnamese local maize cultivar, which is well known for having the highest resistance to weevils among other local cultivars. The ZmDEF1 gene was cloned into a binary vector, pBetaPhaso-dest, which carries phaseolin, a seed-specific promoter, to direct defensin expression in tobacco seeds. …
Alteration In The Subcellular Location Of The Inhibitor Of Growth Proteinp33(Ing1b) In Estrogen Receptor Alpha Positive Breast Carcinoma Cells, İmge Kunter, Emi̇ne Kandemi̇ş, Hani̇ Alotai̇bi̇, Tülay Canda, Şeri̇fe Esra Erdal Bağriyanik
Alteration In The Subcellular Location Of The Inhibitor Of Growth Proteinp33(Ing1b) In Estrogen Receptor Alpha Positive Breast Carcinoma Cells, İmge Kunter, Emi̇ne Kandemi̇ş, Hani̇ Alotai̇bi̇, Tülay Canda, Şeri̇fe Esra Erdal Bağriyanik
Turkish Journal of Biology
ING1 has regulatory roles in the expression of genes associated with proliferation, apoptosis, and senescence. p33(ING1b) is the most widely expressed isoform of the gene. Downregulation of its nuclear expression is involved in differentiation and pathogenesis in invasive breast carcinoma. Yet the mechanism(s) by which p33 nuclear targeting is regulated remains unknown. In this study, we analyzed human invasive breast carcinoma tissue samples by immunostaining with p33 and correlating p33 location with the presence of ERα. Our findings show the expression of p33 protein in ERα-positive tumor samples was in the nucleus alone, while the expression was mainly in the …
Studies On Organogenesis During Regeneration In The Earthworm, Eudrilus Eugeniae, In Support Of Symbiotic Association With Bacillus Endophyticus, Elaiya Raja Subramanian, Dineshkumar Sudalaimani, Johnson Retnaraj Samuel Selvan Christyraj, Kalidas Ramamoorthy M, Nino Gopi D, Jackson Durairaj Selvan Christyraj, Kasimani Renganathan, Sundar Krishnan, Sudhakar Sivasubramaniam
Studies On Organogenesis During Regeneration In The Earthworm, Eudrilus Eugeniae, In Support Of Symbiotic Association With Bacillus Endophyticus, Elaiya Raja Subramanian, Dineshkumar Sudalaimani, Johnson Retnaraj Samuel Selvan Christyraj, Kalidas Ramamoorthy M, Nino Gopi D, Jackson Durairaj Selvan Christyraj, Kasimani Renganathan, Sundar Krishnan, Sudhakar Sivasubramaniam
Turkish Journal of Biology
Organogenesis and animal adaptation upon loss of body parts are crucial events in regeneration of all traits, and both are uncovered areas in biology. The organogenesis of the mouth and anus during regeneration was studied in Eudrilus eugeniae. It was found that the earthworm regenerates a functional mouth and anus in six days. During the course of organogenesis, the earthworm adopts unique starvation behavior for 5 days, and the starvation triggers the gut microflora of E. eugeniae to produce riboflavin. One of the efficient riboflavin-producing bacteria, Bacillus endophyticus, confirmed through 16S rRNA sequencing, has been isolated from its gut. The …
Cloning And Characterization Of A Serine/Threonine Protein Phosphatase2a-Encoding Gene Ibpp2a1 From Ipomoea Batatas (L.) Lam., Pan Xu, Bin Yong, Huan-Huan Shao, Jiabin Shen, Bin He, Qinqin Ma, Xianghua Yuan, Yu Wang
Cloning And Characterization Of A Serine/Threonine Protein Phosphatase2a-Encoding Gene Ibpp2a1 From Ipomoea Batatas (L.) Lam., Pan Xu, Bin Yong, Huan-Huan Shao, Jiabin Shen, Bin He, Qinqin Ma, Xianghua Yuan, Yu Wang
Turkish Journal of Biology
Sweet potato is one of the most important food crops with strong environmental adaptability. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that plays crucial roles in hormone signal transduction and abiotic stress response. Characterization of PP2A can elucidate the mechanisms of stress resistance in this crop. In this study, a total of 15 PP2A transcripts, including nine regulatory subunit-encoding sequences and six catalytic subunit-encoding sequences, were identified from sweet potato and found to be expressed at varying levels. Only one contained a complete open reading frame and encoded a B regulatory subunit (termed IbPP2A1). Ten polymorphic sites …
Lipocalin Gene Expression Is Varied In Developmental Stages By Larval Nutritional Stress In Drosophila, Nazli Ayhan, Pinar Güler, Banu Şebnem Önder
Lipocalin Gene Expression Is Varied In Developmental Stages By Larval Nutritional Stress In Drosophila, Nazli Ayhan, Pinar Güler, Banu Şebnem Önder
Turkish Journal of Biology
Lipocalin genes NLaz, GLaz, and Karl are evolutionarily conserved genes in Drosophila melanogaster. There are studies on lipocalin gene expression differences under diverse diet conditions, but these studies have focused mainly on age-dependent expression profiles of these genes. The main aim of our study is to determine lipocalin expression in the developmental period by nutritional manipulation with an isofemale-based design. Three larval developmental periods have been researched under normal and restricted diets. We found significant differences between lines during their developmental time-related lipocalin expression. Here, we demonstrate that upregulations in the early developmental stages of lipocalin genes under stressful conditions …
Differential Oxidative Response To Fluoropyrimidines In Colorectal Cancer Cell Lines, Ufuk Özer, Karen Wood Barbour
Differential Oxidative Response To Fluoropyrimidines In Colorectal Cancer Cell Lines, Ufuk Özer, Karen Wood Barbour
Turkish Journal of Biology
Colorectal cancer (CRC) is the second most common human malignancy in women and the third in men, accounting for approximately 8% of cancer-related deaths and 1.3 million newly diagnosed cases annually worldwide. CRC cell lines differ in morphological features, growth rate, and their genetic alterations, implicating the extent of variability among the lines. The goal of the current study was to investigate the phenotypes of CRC lines related to NADPH oxidase (NOX) activity and expression of NOX2 subunits in response to fluoropyrimidines. NOX activity in response to 5'-fluoro-2'-deoxyuridine (FdUrd) and VAS2780 was measured in CRC cell lines. Cells were treated …
Wild-Growing Rosa Heckeliana Tratt.: Phenolic Constituentswith Cytotoxic And Antioxidative Properties, Nursen Çoruh, Ni̇zametti̇n Özdoğan
Wild-Growing Rosa Heckeliana Tratt.: Phenolic Constituentswith Cytotoxic And Antioxidative Properties, Nursen Çoruh, Ni̇zametti̇n Özdoğan
Turkish Journal of Biology
Rosa heckeliana, as one of the wild-growing species of the family Rosacea, has a wide use in the folk medicine; however, scientifically there is very little known about it. Our objective was to examine the antioxidative and antiproliferative properties of Rosa heckeliana root extract and its phenolics. The phenolic constituents, namely catechin, caffeic acid, and ellagic acid, were obtained by the methods of extraction, fractionation, and purification through column chromatography. The DPPH and ABTS radical scavenging assays and total flavonoid content analysis were employed as the current antioxidant methods over the crude extract and fractionated parts. Among the extracts/fractions, the …
Cryopreservation Of Citrus Seed Via Dehydration Followedby Immersion In Liquid Nitrogen, Ergun Kaya, Fernanda Souza, Emel Yilmaz Gökdoğan, Muammer Ceylan, Maria Jenderek
Cryopreservation Of Citrus Seed Via Dehydration Followedby Immersion In Liquid Nitrogen, Ergun Kaya, Fernanda Souza, Emel Yilmaz Gökdoğan, Muammer Ceylan, Maria Jenderek
Turkish Journal of Biology
An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. x Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability …
Population Variation In Drought Resistance And Its Relationship With Adaptiveand Physiological Seedling Traits In Turkish Red Pine (Pinus Brutia Ten.), Gaye Kandemi̇r, Sertaç Önde, Fati̇h Temel, Zeki̇ Kaya
Population Variation In Drought Resistance And Its Relationship With Adaptiveand Physiological Seedling Traits In Turkish Red Pine (Pinus Brutia Ten.), Gaye Kandemi̇r, Sertaç Önde, Fati̇h Temel, Zeki̇ Kaya
Turkish Journal of Biology
Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 …
Microrna-34a Regulates Brain-Derived Neurotrophic Factorin An Intracerebral Hemorrhage Model, Jin Hee Kim, Jae-Sun Choi
Microrna-34a Regulates Brain-Derived Neurotrophic Factorin An Intracerebral Hemorrhage Model, Jin Hee Kim, Jae-Sun Choi
Turkish Journal of Biology
Intracerebral hemorrhages (ICHs) are devastating neurological events frequently resulting in a serious negative prognosis. The exact physiological and disease processes involved in ICHs are complex, but are thought to involve microRNAs (miRNAs), 22 nucleotide small noncoding RNAs that control a variety of normal physiological and disease processes. In this study, we show that a miRNA, miR-34a, regulates BDNF in a model of ICH injury. In particular, we assessed the impact of AM34a, an inhibitor of miR-34a, on the toxicity of thrombin-induced apoptosis and on BDNF-mediated signaling. We investigated the increased expression of miR-34a after an ICH-induced thrombin toxicity injury using …
Neuropeptide Y Reduces Migration Capacity Of Human Choriocarcinomacell Line By Altering Oxidative/Antioxidative Status, Milos Matic, Milica Paunovic, Branka Ognjanovic, Andras Stajn, Zorica Saicic
Neuropeptide Y Reduces Migration Capacity Of Human Choriocarcinomacell Line By Altering Oxidative/Antioxidative Status, Milos Matic, Milica Paunovic, Branka Ognjanovic, Andras Stajn, Zorica Saicic
Turkish Journal of Biology
Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term …
The Comparison Of Antioxidant Capacity And Cytotoxic, Anticarcinogenic,And Genotoxic Effects Of Fe@Au Nanosphere Magnetic Nanoparticles, Hande Yegenoglu, Belma Aslim, Burcu Guven, Adem Zengin, İsmai̇l Hakki Boyaci, Zeki̇ye Suludere, Uğur Tamer
The Comparison Of Antioxidant Capacity And Cytotoxic, Anticarcinogenic,And Genotoxic Effects Of Fe@Au Nanosphere Magnetic Nanoparticles, Hande Yegenoglu, Belma Aslim, Burcu Guven, Adem Zengin, İsmai̇l Hakki Boyaci, Zeki̇ye Suludere, Uğur Tamer
Turkish Journal of Biology
Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast …
Heterologous Expression And Characterization Of A High Redox Potential Laccase Fromcoriolopsis Polyzona Mucl 38443, Orkun Pi̇nar, Candan Tamerler Behar, Ayten Karataş
Heterologous Expression And Characterization Of A High Redox Potential Laccase Fromcoriolopsis Polyzona Mucl 38443, Orkun Pi̇nar, Candan Tamerler Behar, Ayten Karataş
Turkish Journal of Biology
In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 …
Genetic Engineering Of An Industrial Strain Of Streptomyces Clavuligerusfor Further Enhancement Of Clavulanic Acid Production, Aslihan Kurt Kizildoğan, Güli̇z Vanli Jaccard, Alper Mutlu, İbrahi̇m Sertdemi̇r, Gülay Özcengi̇z
Genetic Engineering Of An Industrial Strain Of Streptomyces Clavuligerusfor Further Enhancement Of Clavulanic Acid Production, Aslihan Kurt Kizildoğan, Güli̇z Vanli Jaccard, Alper Mutlu, İbrahi̇m Sertdemi̇r, Gülay Özcengi̇z
Turkish Journal of Biology
An industrial clavulanic acid (CA) overproducer Streptomyces clavuligerus strain, namely DEPA, was engineered to further enhance its CA production. Single or multiple copies of ccaR, claR (pathway-specific activators), and cas2 (CA synthase) genes under the control of different promoters were introduced into this strain. CA titers of the resulting recombinants were analyzed by HPLC in a dynamic fashion and compared to the vector-only controls and a wild-type strain of S. clavuligerus while their growth was monitored throughout fermentation. The addition of an extra copy of ccaR, under control of its own promoter or constitutive ermE* promoter (PermE*), led to 7.6- …
Fret-Based Characterization Of K264a, D345a, And Y335amutants In The Human Dopamine Transporter, Oya Orun, Pinar Mega Ti̇ber
Fret-Based Characterization Of K264a, D345a, And Y335amutants In The Human Dopamine Transporter, Oya Orun, Pinar Mega Ti̇ber
Turkish Journal of Biology
The dopamine transporter (DAT) plays a role in the termination of dopaminergic neurotransmission; thereby it is accepted as the primary target of various psychostimulants. N-terminal phosphorylation of DAT has been proposed as a regulator in different DAT functions, such as amphetamine-induced efflux or PKC/PKA-mediated responses. To understand the role of N-terminal conformational changes in dopamine transporter structure and function, the fluorescence resonance energy transfer (FRET) method was applied to various DAT constructs fluorescently labeled at the N-terminus and substrate-induced conformational changes were determined using rhodamine-labeled cocaine analog JHC1-64 in three DAT mutants. The results indicated that the construct with YFP …
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Turkish Journal of Biology
In view of the increasing demand for essential oils from medicinal and aromatic plants, the application of irradiated polysaccharides like chitosan is a promising option. A pot experiment was performed to study the influence of various concentrations of irradiated chitosan (ICN) on growth, physiological, and yield parameters of Mentha piperita L., a plant with considerable pharmaceutical importance. The treatments were foliar spray of deionized water only (control), foliar spray of unirradiated chitosan (40 mg L-1), and ICN at 40, 80, 120, and 160 mg L-1. Among these, 80 mg L-1 ICN (treatment 4) proved to be the best for most …
Reference Gene Expression In Human Osteosarcoma Cell Lines Treated By Egb And Ctx, Ming Ren, Qiwei Yang, Yuanyuan Song, Ao Wang, Qingyu Wang, Xiaonan Wang, Shuhong Hao, Zhitao Wang, Zhenwu Du, Guizhen Zhang, Jincheng Wang
Reference Gene Expression In Human Osteosarcoma Cell Lines Treated By Egb And Ctx, Ming Ren, Qiwei Yang, Yuanyuan Song, Ao Wang, Qingyu Wang, Xiaonan Wang, Shuhong Hao, Zhitao Wang, Zhenwu Du, Guizhen Zhang, Jincheng Wang
Turkish Journal of Biology
The selection of stably expressed reference genes is crucial for quantitative real-time polymerase chain reaction (qRT-PCR) assay when assessing osteosarcoma treated with cyclophosphamide (CTX) and Ginkgo biloba L. extract (EGB). We aimed to identify the reference genes that are stably expressed in human osteosarcoma cell lines after the above treatments. We used qRT-PCR assay to determine the expression levels of 18S, ACTB, ALAS1, GAPDH, TBP, HPRT1, RPL29, HMBS, PPIA, PUM1, GUSB, and B2M in the cell lines MG-63, Saos-2, and U2OS, which were treated with CTX or EGB or both. We analyzed the expression stability of these genes using geNorm, …
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Turkish Journal of Biology
The critical period of development is highly susceptible to disorders. Environmental contaminants, stress, and poor nutrition may permanently affect structurally and functionally an organism during adulthood. Protein restriction in intrauterine and neonatal periods may impair testicular cells and reduce steroidogenic activity. The current study investigated the effect of low protein diet during intrauterine and postnatal life on testicular function in immature and adult rats. Pregnant Wistar rats were fed either a normal protein diet or a low protein diet during pregnancy until birth and during lactation until weaning. The results showed that undernutrition during intrauterine life reduced epithelium height, tubular …
Identification And In Silico Analysis Of Galns Mutations Causing Morquio A Syndrome In Eight Consanguineous Families, Irfan Ullah, Abdul Nasir, Sarmad Mehmood, Sohail Ahmed, Muhammad Ikram Ullah, Asmat Ullah, Abdul Aziz, Syed Irfan Raza, Khadim Shah, Saad Ullah Khan, Muhammad Jawad Hassan, Wasim Ahmad
Identification And In Silico Analysis Of Galns Mutations Causing Morquio A Syndrome In Eight Consanguineous Families, Irfan Ullah, Abdul Nasir, Sarmad Mehmood, Sohail Ahmed, Muhammad Ikram Ullah, Asmat Ullah, Abdul Aziz, Syed Irfan Raza, Khadim Shah, Saad Ullah Khan, Muhammad Jawad Hassan, Wasim Ahmad
Turkish Journal of Biology
Genetic deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) due to mutations in the GALNS gene results in the pathogenesis of Morquio A syndrome. To date, more than 200 mutations have been reported in GALNS, resulting in variable clinical features. For this reason, bioinformatics analysis of these mutations is important to determine their effect on the structure and functions of the protein and to establish a correlation between genotype and phenotype. In the present study, eight Pakistani consanguineous families with Morquio A syndrome were clinically and genetically evaluated. Linkage analysis followed by sequence analysis of the gene detected four novel (p.Phe216Ser, p.Met38Arg, p.Ala291Ser, …
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Turkish Journal of Biology
The present investigation was carried out for the induction of haploid plants in Citrus grandis through in situ parthenogenesis by pollination with gamma-irradiated pollen of C. limetta and C. sinensis, treated with 50, 100, 200, 300, and 400 Gy gamma ray doses followed by in vitro ovule culture. Ovule culture at 50 days after pollination (DAP) was found optimum for the maximum recovery of in vitro-raised plantlets as compared to 35 and 20 DAP. At 50 DAP, irrespective of the pollen parent, plantlet regeneration capacity decreased at 300 and 400 Gy irradiation treatment (0.62% and 0.60%, respectively) with maximum recovery …
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Turkish Journal of Biology
Cell compatibility is one of the prominent requirements for the fabrication of tissue engineering materials. Silk fibroin (SF) is an excellent material for biomedical applications and shows desirable properties such as good compatibility, minimal tissue reaction, and tailorable degradability. Poly[(R)-3-hydroxybutyrate] (PHB) has a high percentage of crystallinity and a high melting temperature. In this study, PHB and SF were blended together to improve the mechanical properties of the SF fibrous structure and the crystallinity of PHB. Furthermore, the cell-biomaterial interaction on the PHB/SF composite scaffold was expected to be enhanced via the SF. For this purpose, PHB/SF composite scaffolds were …
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
Turkish Journal of Biology
This study reports the generation of a new human muscle tissue equivalent from skeletal muscle-derived stem cells and human umbilical vein endothelial cells (HUVECs). Skeletal muscle stem cells were isolated by the preplate technique and differentiated into neuron-like cells that were positive for neuronal beta-tubulin3 and nestin and negative for the astrocyte marker glial fibrillary acidic protein (GFAP). Coculture of skeletal muscle stem cells with the HUVECs under optimized fetal bovine serum and media conditions resulted in formation of a capillary network among the multinucleated myotubes. The neuron-like cells derived from the human skeletal muscle stem cells were seeded onto …
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
Turkish Journal of Biology
Tartary buckwheat is a type of coarse cereal that contains a rich supply of flavonoids. When suffering from certain stresses, tartary buckwheat can respond by promoting the biosynthesis of anthocyanins. It is well established that the jasmonate (JA) signaling pathway plays a critical role in plant resistance to disease by stimulating the production of anthocyanins; furthermore, the genes encoding the jasmonate-ZIM domain (JAZ) proteins have been shown to be key players in this process. However, the role of JAZ proteins in tartary buckwheat is poorly understood. In this study, we aimed to isolate FtJAZs from tartary buckwheat and identify FtJAZs …
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Turkish Journal of Biology
This study aims to explore the role and the possible underlying molecular mechanism of SNF5 in the pathogenesis of rheumatoid arthritis (RA). MH7A cells were respectively transfected with pc-SNF5 (pcDNA3.1 containing the Brg1 coding sequence), short hairpin RNA against SNF5 (shSNF5), and their negative controls (pcDNA3.1 and shNC). The alterations of SNF5 expression were assessed by qRT-PCR and western blot analysis. MTT assay, flow cytometry, and western blot analysis were performed to evaluate proliferation, apoptosis, and expression levels of p16 and JNK pathway associated proteins, respectively. Finally, the effect of SNF5 was verified in fibroblast-like synoviocytes (FLSs) obtained from a …
Analysis Of Ca125 Antigen In Normal Human Seminal Plasma Highlightsthe Molecular Heterogeneity Of Underlying Glycosylated Species, Ninoslav Mitic, Bojana Milutinovic, Miroslava Jankovic
Analysis Of Ca125 Antigen In Normal Human Seminal Plasma Highlightsthe Molecular Heterogeneity Of Underlying Glycosylated Species, Ninoslav Mitic, Bojana Milutinovic, Miroslava Jankovic
Turkish Journal of Biology
Cancer antigen CA125 represents an extracellular part of transmembrane mucin MUC16 and may be expressed in tissues of the male reproductive tract. It reaches human seminal plasma (hSP) after undergoing the main processes involved in protein speciation: synthesis, posttranslational modifications, compartmentalization, and auto/proteolytic degradation. This study was aimed at profiling CA125-immunoreactive species in hSP from healthy subjects as a specific and unexplored source of this tumor-associated antigen expressed under normal physiological conditions. High molecular mass components from total hSP and corresponding acid-soluble hSP preparations were analyzed. The results indicated that antibodies recognizing two distinct immunogenic areas of CA125 antigen exhibited …
Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Turkish Journal of Biology
Leaf rolling (LR), particularly in crops, is a dehydration avoidance mechanism that results from water loss in plants under drought conditions. Two maize cultivars (Zea mays L.) differing in their tolerance to drought (cultivar Side was selected as stress-tolerant and cultivar Karaçay was selected as stress-sensitive) under PEG-induced drought stress were used in this study to understand the effects of polyamines (PAs) on LR response and the role of the antioxidant system in the delayed LR process. Aerial parts of the seedlings were excised prior to the experiment and then submerged in a Hoagland nutrient solution with or without 0.1 …
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Turkish Journal of Biology
The present study is the first research on the phytotoxicological effects of Er-doped zinc oxide nanoparticles (NPs) on duckweed (Lemna minor L.), as a model aquatic floating macrophyte. It concentrated on the comparison of the physiological effects of different concentrations of undoped and Er-doped zinc oxide NPs on L. minor. Pure and Er-doped zinc oxide samples were synthesized through a sonochemical method and their morphology and chemical composition were studied by scanning electron microscopy and energy dispersive X-ray analysis. Plant growth, photosynthetic pigment contents, and antioxidant enzyme activities were investigated as indices to assess the effects and toxicity of the …