Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- University of Nebraska - Lincoln (7)
- Old Dominion University (6)
- Aga Khan University (3)
- Iowa State University (3)
- Loyola University Chicago (3)
-
- Wayne State University (3)
- Dartmouth College (2)
- Illinois Wesleyan University (2)
- University of Montana (2)
- Cleveland State University (1)
- Marshall University (1)
- Selected Works (1)
- University of Massachusetts Amherst (1)
- University of the Pacific (1)
- Utah State University (1)
- Valparaiso University (1)
- Western Michigan University (1)
- William & Mary (1)
- Keyword
-
- Isoenzymes (2)
- Methotrexate (2)
- 5-biphosphate carboxylase (1)
- <i>Chlamydomonas reinhardtii</i> (1)
- AIDS (Disease) (1)
-
- Adrenal glands (1)
- Air pollutants (1)
- Animals (1)
- Balanced polymorphism (1)
- Base sequence (1)
- Biochemical (1)
- Blotting (1)
- Carrier proteins (1)
- Cell line (1)
- Cells (1)
- Chloramphenicol o-acetyltransferase (1)
- Chromatography (1)
- Chronic renal failure (1)
- Circular dichroism (1)
- Collaboration (1)
- Cyclic AMP (1)
- Cytotoxicity (1)
- Dna probes (1)
- Dolichol phosphates (1)
- Drug effects (1)
- Energy resources (1)
- Environmental assessment (1)
- Environmental legislation (1)
- Enzyme induction (1)
- Forest resources (1)
- Publication
-
- Department of Biochemistry: Faculty Publications (5)
- Chemistry & Biochemistry Theses & Dissertations (4)
- Biochemistry and Molecular Biology Faculty Publications (3)
- David J. Oliver (3)
- Department of Biological & Biomedical Sciences (3)
-
- Bioelectrics Publications (2)
- Chemistry and Biochemistry Faculty Publications (2)
- Dartmouth Scholarship (2)
- David Hage Publications (2)
- Dissertations (2)
- Scholarship (2)
- All Dugoni School of Dentistry Faculty Articles (1)
- All Graduate Theses and Dissertations, Spring 1920 to Summer 2023 (1)
- Biological Sciences Faculty Research (1)
- Biology Faculty Publications (1)
- Chemistry: Faculty Publications and Other Works (1)
- Dissertations, Theses, and Masters Projects (1)
- Gail Gasparich (1)
- Lila Gierasch (1)
- Masters Theses (1)
- Physics Faculty Publications (1)
- Publication Type
Articles 31 - 40 of 40
Full-Text Articles in Life Sciences
Intact Parathyroid Hormone: Performance And Clinical Utility Of An Automated Assay Based On High-Performance Immunoaffinity Chromatography And Chemiluminescence Detection, David S. Hage, Bob Taylor, Pai C. Kao
Intact Parathyroid Hormone: Performance And Clinical Utility Of An Automated Assay Based On High-Performance Immunoaffinity Chromatography And Chemiluminescence Detection, David S. Hage, Bob Taylor, Pai C. Kao
David Hage Publications
The performance and clinical utility of an automated assay of intact parathyroid hormone (parathyrin, PTH) are evaluated. The method is based on the extraction of PTH from plasma by an HPLC column containing immobilized anti-(44-68 PTH) antibodies. The PTH retained is detected with a postcolumn reactor and use of anti-(1--34 PTH) chemiluminescent-labeled antibodies. The total cycle time of the assay is 6.5 mm per injection after a 1-h incubation.The lower limit of detection for PTH in a 66-pL plasma sample was 0.5 pmol/L based on peak heights and 0.2 pmol/L based on peak areas. Mean analytical recovery for PTH added …
Glossary For Chemists Of Terms Used In Biotechnology, B. Nagel, H. Dellweg, Lila Gierasch
Glossary For Chemists Of Terms Used In Biotechnology, B. Nagel, H. Dellweg, Lila Gierasch
Lila Gierasch
The glossary contains definitions and explanatory notes, if needed, of over 230 terms frequently used in publications in the multidisciplinary field of biotechnology. The glossary was developed as a step to help facilitate communication between chemists, chemical engineers, biologists and bioengineers and to make biotechnology and its methodologies more accessible to the chemical profession. The interrelation between chemistry, chemical engineering and the burgeoning areas of molecular biology will be especially important as chemical industrial processes begin to incorporate recombinant DNA techniques, for example. The range of terms includes microbiology, genetic engineering, biochemistry, molecular biology, biochemical engineering, bioprocessing and general concepts …
The Cγ Subunit Is A Unique Isozyme Of The Camp-Dependent Protein Kinase, Stephen J. Beebe, Paul Salomonsky, Tore Jahnsen, Yixin Li
The Cγ Subunit Is A Unique Isozyme Of The Camp-Dependent Protein Kinase, Stephen J. Beebe, Paul Salomonsky, Tore Jahnsen, Yixin Li
Bioelectrics Publications
There are at least three isozymes (Cα, Cβ, and Cγ) of the mammalian catalytic (C) subunit of cAMP-dependent protein kinase (PKA) (Beebe, S., Oyen, O., Sandberg, M., Froysa, A., Hansson, V., and Jahnsen, T. (1990) Mol. Endocrinol. 4, 465-475). To compare the Cγ and Cα isozymes, the respective cDNAs were expressed in permanently transformed Kin-8 PKA-deficient Y1 adrenal cells using the mouse metallothionein promoter. The recombinant C subunits were characterized as immunoreactive, zinc-inducible, cAMP-dependent kinase activities. In contrast to Cα, histone was a better substrate than Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide) for Cγ. Furthermore, Cγ histone kinase activity was not inhibited by the …
Lack Of Types 1 And 2a Protein Serine(P)/Threonine(P) Phosphatase Activities In Chloroplasts, Gongqin Sun, John Markwell
Lack Of Types 1 And 2a Protein Serine(P)/Threonine(P) Phosphatase Activities In Chloroplasts, Gongqin Sun, John Markwell
Department of Biochemistry: Faculty Publications
Protein phosphatase activity in crude leaf extracts and in purified intact chloroplasts of wheat (Triticum aestivum) and pea (Pisum sativum) was analyzed using exogenously supplied phosphoproteins or endogenous thylakoid proteins. Leaf extracts contain readily detectable amounts of protein phosphatase activity measured with either phosphohistone or phosphorylase a, substrates of mammalian protein phosphatases. No significant chloroplast protein phosphatase activity was detected using these exogenous phosphoproteins. The dephosphorylation of endogenous thylakoid lightharvesting chlorophyll a/b binding proteins in situ was inhibited by fluoride, but not by microcystin-LR or okadaic acid, diagnostic inhibitors of mammalian types 1 and 2A protein …
Cytophotometric Estimation Of Nuclear Dna Content Variation In Ten Species Of Geniculate Coralline Algae (Corallinaceae, Rhodophyta), Jeffrey Craig Bailey
Cytophotometric Estimation Of Nuclear Dna Content Variation In Ten Species Of Geniculate Coralline Algae (Corallinaceae, Rhodophyta), Jeffrey Craig Bailey
Dissertations, Theses, and Masters Projects
No abstract provided.
A Proposal For United States/Polish Collaboration On Energy And The Environment, Carole B. Szpunar
A Proposal For United States/Polish Collaboration On Energy And The Environment, Carole B. Szpunar
Chemistry: Faculty Publications and Other Works
Argonne National Laboratory, a U.S. Department of Energy facility that is operated by the University of Chicago and located southwest of Chicago, Illinois, has proposed a collaborative effort by the United States and Poland to conduct applied research on energy and environmental topics of common interest. Through this collaboration, evaluative studies on energy and environmental assessment issues could be developed and implemented by Argonne and one or more sister institutions in Poland, which could lead to active research, development, and demonstration joint activities. The objective of this initiative is to produce strategies and options for review by Poland's decision makers …
Monoclonal Antibodies Specific For Mercuric Ions, Dwane E. Wylie, Di Lu, Larry D. Carlson, Randy Carlson, K. Funda Babacan, Sheldon M. Schuster, Fred W. Wagner
Monoclonal Antibodies Specific For Mercuric Ions, Dwane E. Wylie, Di Lu, Larry D. Carlson, Randy Carlson, K. Funda Babacan, Sheldon M. Schuster, Fred W. Wagner
Department of Biochemistry: Faculty Publications
Monoclonal antibodies (mAbs) that react with soluble mercuric ions have been produced by i 'ection of BALB/c mice with a hapten-carrier complex designed to maximize exposure of the metal to the immune system. Three hybridomas producing antibodies that reacted with bovine serum albumin (BSA)-glutathione-HgCI, but not with BSAglutathione, were isolated from the spleen of a mouse given multiple iqjections with glutathione-HgCl conjugated to keyhole limpet hemocyanin. Stable subclones were established from two of these antibodies, designated mAb 4A10 and mAb IF10. The binding of both antibodies to immobilized BSAglutathione- HgCl was inhibited by soluble HgCl2, and dissociation constants …
Chloroplast Heteroplasmicity Is Stabilized By An Amber-Suppressor Tryptophan TrnaCua, Weizhu Yu, Robert J. Spreitzer
Chloroplast Heteroplasmicity Is Stabilized By An Amber-Suppressor Tryptophan TrnaCua, Weizhu Yu, Robert J. Spreitzer
Department of Biochemistry: Faculty Publications
Photosynthesis-deficient mutants of the green alga Chlamydomonas reinhardtii were previously shown to arise from nonsense mutations within the chloroplast rbcL gene, which encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39). Photosynthesiscompetent revertants of these nonsense mutants have often been found to be stably heteroplasmic, giving rise to both mutant and revertant cells during mitotic or meiotic divisions under nonselective growth conditions. It was proposed that the stable heteroplasmicity might arise from a balanced polymorphism of suppressor and wild-type alleles of a tRNA gene maintained within the polyploid chloroplast genome. In the present study, we have focused on determining the …
Transition Metals In Legume Root Nodules: Iron-Dependent Free Radical Production Increases During Nodule Senescence, Manuel Becana, Robert V. Klucas
Transition Metals In Legume Root Nodules: Iron-Dependent Free Radical Production Increases During Nodule Senescence, Manuel Becana, Robert V. Klucas
Department of Biochemistry: Faculty Publications
The cytosol from root nodules of soybean, bean, and cowpea contained Fe and Cu capable of catalyzing the formation of highly reactive free radicals. Specific and sensitive assays based on free radical-mediated DNA degradation revealed that most catalytic Fe and Cu were present as small chelates (300-600 Da). The involvement of catalytic Fe in free radical production during nodule senescence, which was induced by exposure of plants to continuous darkness for 2-4 days, was investigated. (i) Free heme remained at a constant and low concentration (1-4% of total nodule heme) during senescence, indicating that it is not an …
Light Activation Of Maize Phosphoenolpyruvate Carboxylase Protein-Serine Kinase Activity Is Inhibited By Mesophyll And Bundle Sheath-Directed Photosynthesis Inhibitors, Jin-An Jiao, Raymond Chollet
Light Activation Of Maize Phosphoenolpyruvate Carboxylase Protein-Serine Kinase Activity Is Inhibited By Mesophyll And Bundle Sheath-Directed Photosynthesis Inhibitors, Jin-An Jiao, Raymond Chollet
Department of Biochemistry: Faculty Publications
C4 phosphoenolpyruvate carboxylase (PEPC) is post-translationally regulated by reversible phosphorylation of a specific N-terminal seryl residue in response to light/dark transitions of the parent leaf tissue. The protein-serine kinase (PEPC-PK) that phosphorylates/activates this mesophyll-cytoplasm target enzyme is slowly, but strikingly, activated by high light and inactivated in darkness in vivo by a mechanism involving cytoplasmic protein synthesis/degradation as a primary component. In this report, evidence is presented indicating that the inhibition of Calvin cycle activity by a variety of mesophyll (3-(3,4-dichlorophenyl)-1,1-dimethylurea, isocil, methyl viologen) and bundle sheath (DL-glyceraldehyde)-directed photosynthesis inhibitors blocks the light activation of maize (Zea mays …