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Articles 1 - 30 of 31
Full-Text Articles in Life Sciences
On The Anti-Adipogenic Function Of Collagen Triple Helix Repeat-Containing Protein 1, Matthew E. Siviski
On The Anti-Adipogenic Function Of Collagen Triple Helix Repeat-Containing Protein 1, Matthew E. Siviski
Electronic Theses and Dissertations
Adipogenesis is regulated by the coordinated activity of adipogenic transcription factors, including PPAR-gamma (PPARG) and C/EBP alpha (CEBPA). Thus, dysregulated adipogenesis predisposes adipose tissues to adipocyte hypertrophy and hyperplasia. We have previously reported that mice possessing a homozygous null gene mutation in collagen triple helix repeat-containing protein 1 (CTHRC1) have increased adiposity compared to wildtype mice, supporting the concept that CTHRC1 regulates body composition. Herein, we investigated the anti-adipogenic activity of CTHRC1. Using 3T3-L1 preadipocytes, we showed significantly reduced adipogenic differentiation in the presence of CTHRC1 commensurate to marked suppression of Cebpa and Pparg gene expression. In addition, CTHRC1 increased …
Regulation Of Line-1 In Developing Oocytes And The Impact On The Ovarian Reserve, Rose Besen-Mcnally
Regulation Of Line-1 In Developing Oocytes And The Impact On The Ovarian Reserve, Rose Besen-Mcnally
Electronic Theses and Dissertations
In humans and mice, the ovarian reserve (OR) is established during a lengthy process that starts during early embryogenesis with germ cell specification and culminates in the first weeks after birth when primordial follicles (PF) are formed. OR establishment is an important process which influences the fertile lifespan and fecundity of the individual. Fetal oocyte attrition (FOA) has been identified as a critical developmental event that regulates how many oocytes survive and contribute to the final OR. In addition to FOA, OR size and quality also depend on efficiency of meiotic recombination. Chromosome asynapsis and unrepaired meiotic double-strand breaks (DSB) …
Computationally Modeling Dynamic Biological Systems, Katherine Jarvis
Computationally Modeling Dynamic Biological Systems, Katherine Jarvis
Electronic Theses and Dissertations
Modeling biological systems furthers our understanding of dynamic relationships and helps us make predictions of the unknown properties of the system. The simple interplay between individual species in a dynamic environment over time can be modeled by equation-based modeling or agent- based modeling (ABM). Equation based modeling describes the change in species quantity using ordinary differential equations (ODE) and is dependent on the quantity of other species in the system as well as a predetermined rates of change. Unfortunately, this method of modeling does not model each individual agent in each species over time so individual dynamics are assumed to …
Hells And Prdm9 Form A Pioneer Complex To Open Chromatin At Meiotic Recombination Hotspots: A Technological Perspective, Catrina Spruce
Hells And Prdm9 Form A Pioneer Complex To Open Chromatin At Meiotic Recombination Hotspots: A Technological Perspective, Catrina Spruce
Electronic Theses and Dissertations
Chromatin functions as a physical barrier regulating access to DNA, however pioneer factors are able to engage partial recognition motifs present within nucleosomal DNA to initiate access to specific DNA sequences. During spermatogenesis, genomic locations that become recombination hotspots are generally in regions of closed chromatin, or heterochromatin, before meiosis. However, in leptotene and zygotene stages of meiosis, PRDM9 marks nucleosomes at recombination hotspots with H3K4me3 and H3K36me3 and recruits other factors that deposit additional histone marks. Here I focus on the technological approaches by which we discovered that hotspots also transition from closed to open chromatin, dependent on the …
Examining The Function Of Protein Acyltransferase Via The Dhhc Domain Of The Paz5 Protein In The Organism Dictyostelium Discoideum, George M. Stuart-Ranchev
Examining The Function Of Protein Acyltransferase Via The Dhhc Domain Of The Paz5 Protein In The Organism Dictyostelium Discoideum, George M. Stuart-Ranchev
Electronic Theses and Dissertations
Protein S-palmitoylation plays a crucial role in many biological systems. S-palmitoylation involves the post-translational attachment of palmitate to a cysteine residue through a reversible thioester linkage. S-Palmitoylation is used to modify both integral and membrane proteins, many of which are involved in intracellular trafficking, membrane localization, and signal transduction pathways. Intracellular palmitoylation is mediated by a family of protein acyltransferases (PATs). PAT mutations are associated with neurological diseases and cancer progression. Proteins in the PAT family are defined by the presence of a 51-amino acid cysteine-rich domain (CRD), which contains a highly conserved aspartate-histidine-histidine-cysteine (DHHC) motif. The …
Triclosan Disrupts Immune Cell Function By Depressing Ca2+ Influx Via Acidification Of The Cytoplasm, Suraj Sangroula
Triclosan Disrupts Immune Cell Function By Depressing Ca2+ Influx Via Acidification Of The Cytoplasm, Suraj Sangroula
Electronic Theses and Dissertations
Triclosan (TCS) is an antimicrobial agent that was effectively banned by the FDA from hand soaps in 2016, hospital soaps in 2017, and hand sanitizers in 2019; however, TCS can still be found in a few products. At consumer-relevant, non-cytotoxic doses, TCS inhibits the functions of both mitochondria and mast cells, a ubiquitous cell type. Via the store-operated Ca2+ entry mechanism utilized by many immune cells, mast cells undergo antigen-stimulated Ca2+ influx into the cytosol, for proper function. Previous work showed that TCS inhibits Ca2+ dynamics in mast cells, and here we show that TCS also inhibits …
The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga
The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga
Electronic Theses and Dissertations
Dickeya dianthicola (Samson) causing blackleg and soft rot was first detected in potatoes grown in Maine in 2014. Previous work has suggested that insects, particularly aphids, may be able to vector bacteria in this genus between plants, but no conclusive work has been done to confirm this theory. In order to determine whether insect-mediated transmission is likely to occur in potato fields, two model potato pests common in Maine were used: the Colorado potato beetle (Leptinotarsa decimlineata Say) and the green peach aphids (Myzus persicae Sulzer). Olfactometry and recruitment experiments evaluated if either insect discriminates between infected and …
Dynamic Regulation Of G-Protein Signaling, William C. Simke
Dynamic Regulation Of G-Protein Signaling, William C. Simke
Electronic Theses and Dissertations
G protein-coupled receptors (GPCRs) are involved in numerous signaling processes ranging from neuronal growth to immune cells tracking invaders. GPCR signaling plays a role in many human diseases and thus GPCRs are important drug targets. Yeast respond to mating pheromone using a GPCR signaling system homologous to those used in humans to polarize their cytoskeleton toward the pheromone source. This is accomplished by initializing a MAPK signaling cascade to arrest the cells in mitosis and upregulate expression of chemotropic proteins. Pathway desensitization is accomplished by the Regulator of G-protein Signaling (RGS). RGS abrogates signaling by binding to the active GPCR, …
Cellular And Molecular Mechanisms Of Mapk Signaling During Jc Polyomavirus Infection, Jeanne Dushane
Cellular And Molecular Mechanisms Of Mapk Signaling During Jc Polyomavirus Infection, Jeanne Dushane
Electronic Theses and Dissertations
As obligate intracellular parasites, viruses must infect a host-cell and requisition cellular machinery for viral replication. JC polyomavirus (JCPyV) is a ubiquitous human pathogen that can cause a lytic infection in glial cells of the central nervous system in immunocompromised individuals. In order to initiate infection however, the virus must alter prototypical cellular processes that promote cellular homeostasis. The main driver of these processes are signaling pathways, the means by which the cell interacts and responds to the extracellular environment. Many signaling cascades are responsible for promoting growth, responding to pathogens, initiating differentiation, or inducing cell death. Through the works …
Detrital Protein Contributes To Oyster Nutrition And Growth In The Damariscotta Estuary, Maine, Usa, Cheyenne M. Adams
Detrital Protein Contributes To Oyster Nutrition And Growth In The Damariscotta Estuary, Maine, Usa, Cheyenne M. Adams
Electronic Theses and Dissertations
Oyster aquaculture is an expanding industry that relies on identifying and utilizing natural estuarine conditions for the economically viable production of a filter-feeding crop. The eastern oyster, Crassostrea virginica, is the principal species currently cultured in Maine. In addition to preferentially consumed phytoplankton, various detrital complexes (non-algal and/or non-living organic matter) may provide some nutrition to C. virginica between times of phytoplankton abundance. Here I investigated the importance of detrital proteins in supporting the growth of oysters cultured in the upper Damariscotta Estuary. Oyster aquaculture in this area is highly successful and previous reports indicate that labile detrital protein …
Water Chemistry Dynamics In Four Vernal Pools In Maine, Usa, Lydia H. Kifner
Water Chemistry Dynamics In Four Vernal Pools In Maine, Usa, Lydia H. Kifner
Electronic Theses and Dissertations
Vernal pools are small seasonal wetlands that are a common landscape feature that contribute to biodiversity in northeastern North American forests. However, even basic information about their biogeochemical functions, such as carbon cycling, is limited. Dissolved gas concentrations (CH4, CO2) and other water chemistry parameters were monitored weekly at the bottom and surface of four vernal pools in central and eastern Maine, USA, from April to August 2016. The vernal pools were supersaturated with respect to CH4 and CO2 at all sampling dates and locations. Concentrations of dissolved CH4 and CO2 ranged …
Investigating The Effects Of Particulate Hexavalent Chromium On The Centriole Linkers, Julieta Martino
Investigating The Effects Of Particulate Hexavalent Chromium On The Centriole Linkers, Julieta Martino
Electronic Theses and Dissertations
Particulate hexavalent chromium (Cr(VI)) compounds are human lung carcinogens. However, their carcinogenicity is poorly understood. The best model for Cr(VI)-induced carcinogenesis involves the acquisition of structural and numerical chromosome instability (CIN). Many mechanisms contribute to CIN. Among these, centrosomes play a pivotal role because they dictate proper segregation of chromosomes during cell division. Cr(VI) causes centrosome amplification, a phenotype where cells have extra centrosomes and hence can undergo unequal distribution of chromosomes resulting in CIN. How Cr(VI) induces these abnormalities is unknown. Moreover, whether Cr(VI)-induced centrosome amplification is a permanent phenotypic change is also unknown. This work investigates the permanence …
Physiological And Evolutionary Implications Of The Pattern Of Expression Of Oxygen-Binding Hemoproteins In Antarctic Notothenioid Fishes, Kimberly Borley
Physiological And Evolutionary Implications Of The Pattern Of Expression Of Oxygen-Binding Hemoproteins In Antarctic Notothenioid Fishes, Kimberly Borley
Electronic Theses and Dissertations
Antarctic icefish do not express hemoglobin (Hb). Icefishes possess cardiovascular modifications including increased densities of blood vessels, larger ventricles and increased blood volume compared to red-blooded relatives. In addition to delivering oxygen to tissues, Hb degrades nitric oxide (NO), a small signaling molecule. To investigate the mechanism driving development of icefish cardiovascular characteristics, I present and test the hypothesis that loss of Hb results in increased steady-state levels of NO, triggering downstream signaling pathways such as angiogenesis. I measured NO breakdown products, as a proxy for NO, and found that icefish have higher steady-state levels of NO metabolites in their …
Alteration Of Nucleotide Excision Repair By Estrogens: Implications For Carcinogenesis, Emily Glynn Notch
Alteration Of Nucleotide Excision Repair By Estrogens: Implications For Carcinogenesis, Emily Glynn Notch
Electronic Theses and Dissertations
Estrogens and estrogen mimics represent a wide range of aquatic contaminants that elicit deleterious effects on exposed organisms. Despite well-characterized reproductive effects of environmental estrogens, less is known about non-reproductive impacts of exogenous estrogen exposure. Additionally, estrogens are known carcinogens, implicated in multiple human cancers. Little or no research has examined the effects of xenoestrogens on DNA repair despite being known carcinogens. The goal of this research was to test the hypothesis that aquatic estrogens enhance the effects of environmental mutagens by altering DNA repair. Of particular interest is nucleotide excision repair (NER), the only repair pathway to remove structurally …
Light-Related Photosynthetic Gene Expression And Enzyme Activity In The Heterokont Alga Vaucheria Litorea And Its Symbiotic Partner The Sacoglossan Mollusc Elysia Chlorotica, Kara M. Soule
Electronic Theses and Dissertations
Photosynthesis is comprised of tightly coupled reactions and therefore requires strict matrices of regulation, particularly involving alterations in gene expression and enzyme activity within the nucleus and plastid. Extensive research has been carried out on these light-regulated mechanisms in plants and green algae, however, much less is known in the red algal lineage, including heterokonts. The goal of this study was to investigate the influence of light on photosynthetic gene expression and select enzyme activity in the heterokont alga Vaucheria litorea and its symbiotic partner Elysia chlorotica, a photosynthetic sacoglossan mollusc (sea slug). Elysia chlorotica harbors V. litorea plastids …
Community College Student Participation In Undergraduate Research: An Explanatory Case Study For Faculty And Research Mentors, Dana L. Peterson
Community College Student Participation In Undergraduate Research: An Explanatory Case Study For Faculty And Research Mentors, Dana L. Peterson
Electronic Theses and Dissertations
This study adapted the current model of science undergraduate research experiences (URE's) and applied this novel modification to include community college students. Numerous researchers have examined the efficacy of URE's in improving undergraduate retention and graduation rates, as well as matriculation rates for graduate programs. However, none have detailed the experience for community college students, and few have employed qualitative methodologies to gather relevant descriptive data from URE participants. This study included perspectives elicited from both non-traditional student participants and the established laboratory community. The purpose of this study was to determine the effectiveness of the traditional model for a …
The Ttc7Fsn/Fsn Mutation Results In Hyperactivation Of Lymphocytes And Overproduction Of Il-4 Leading To The Development Of Systemic Autoimmunity, Beth Lindroth Hill
The Ttc7Fsn/Fsn Mutation Results In Hyperactivation Of Lymphocytes And Overproduction Of Il-4 Leading To The Development Of Systemic Autoimmunity, Beth Lindroth Hill
Electronic Theses and Dissertations
Ttc7fsn/fsn mice exhibit systemic autoimmunity characterized by hyperactivated B cells, increased interleukin-4, autoantibodies, kidney disease and reduced lifespan. Because the pathology is similar to systemic lupus erythematosus, Ttc7fsn/fsn mice are a useful model with which to study early events that lead to autoimmune disease. Although the Ttc7fsn mutation has been identified the gene function is unknown. The phenotype of Ttc7fsn/fsn mice mimics the Th2 autoimmunity of the IL-4 transgenic (Erb et al 1997). It was previously unknown whether the over-production of IL-4 was an intrinsic defect of Ttc7fsn/fsn lymphocytes that led to autoimmunity, or, whether …
Notch Regulation Of Human Breat Cancer Progression: Contrasting Roles For Notch Signaling, Christine F. O'Neill
Notch Regulation Of Human Breat Cancer Progression: Contrasting Roles For Notch Signaling, Christine F. O'Neill
Electronic Theses and Dissertations
Notch signaling is associated with activation of either oncogenic or tumor suppressor activities. The human mammary adenocarcinoma cell line, MDA-MB-231, was characterized in vitro and in xenografts in vivo to test the hypothesis that activation of Notch signaling regulates mammary tumor phenotype. Notch 1, Notch2, and Notch4 signaling was compared by stable expression of their constitutively active intracellular domains (ICD). Notch4 activation led to enhanced tumorigenicity, in addition to increased cell proliferation and survival in vitro, whereas the activation of Notch 1 or Notch2 decreased cell proliferation and survival, in which Notch2 increased apoptosis. Stably transfected cell lines were …
Engineering Calpastatin To Develop A Sensor To Detect Active Calpain, Lisa M. Vanhooser
Engineering Calpastatin To Develop A Sensor To Detect Active Calpain, Lisa M. Vanhooser
Electronic Theses and Dissertations
Calpains, Ca2+-activated cysteine proteases are essential for early embryonic development and function in signal transduction, cell adhesion, and apoptosis. Calpains also contribute to cataractogenesis, myocardial infarctions, and neurodegenerative diseases such as Alzheimer's. The various methods currently available to demonstrate these roles do not directly identify spatial or temporal activation of calpain in cells. Therefore, a tool to detect active calpain in situ will be useful. Calpastatin is the ubiquitous, endogenous inhibitor that specifically binds the active conformation of the conventional calpains. Calpastatin consists of four homologous domains each containing three subdomains A, B, and C. The crystal structure …
Semi-Global Analysis Of The Early Cold Stress Response Transcriptome Of Developing Seedlings Of Rice (Oryzasativa L.,Japonica), Chen Cheng
Electronic Theses and Dissertations
Plants are either sensitive or insensitive to low temperatures. Cool-season species acclimate to chilling and develop tolerance to freezing. Warm-season species vary in the degree of sensitivity to chilling and are not capable of acclimation. Both freezing and chilling tolerance involve the activity of an intricately coordinated network of genes. The regulatory network that defines cold acclimation and freezing tolerance is well understood in Arabidopsis. The centerpiece of this network is a group of transcriptional activators that coordinate a battery of downstream defense-related genes. In contrast, little is known about the corresponding regulon in non-acclimating species. This study utilized …
Structure-Function Analysis Of Nrage: A Protein Involved In Developmental Neural Apoptosis, Rebecca Cowling
Structure-Function Analysis Of Nrage: A Protein Involved In Developmental Neural Apoptosis, Rebecca Cowling
Electronic Theses and Dissertations
The Neurotrophins and Bone Morphogenic Proteins (BMP) have both been implicated in the maintenance of cellular proliferation and apoptosis in the developing nervous system. Downstream of both signaling pathways is NRAGE, a member of the Melanoma Antigen (MAGE) gene family and, under BMP stimulation, activates p38 leading to caspase 3 cleavage. NRAGE possesses a highly conserved MAGE Homology Domain (MHD) and a second, less well conserved MHD (denoted MHD2) as well as a unique 25 tandem WQXPXX hexapeptide repeat region. Binding partners for the MHD and hexapeptide repeat regions have been identified but their cellular consequences have not been defined. …
Nrage Regulates Life And Death Of Neural Progenitors, Stephen E. Kendall
Nrage Regulates Life And Death Of Neural Progenitors, Stephen E. Kendall
Electronic Theses and Dissertations
The development of the central nervous system requires the orchestration of numerous instructive and permissive cues. These factors are secreted from signaling centers and function in a concentration dependent manner that effects the proliferation, survival and differentiation of neural progenitors (NP) and their differentiated progeny. The family including bone morphogenetic proteins (BMPs) are key regulators of NP expansion and survival. However, once NP become committed to a neuronal fate and begin to differentiate they compete for limited amounts of neurotrophin that facilitate further growth and survival. Here we show that the p75 neurotrophin receptor interacting protein NRAGE is expressed in …
Structural And Kinetic Characterization Of Myoglobins From Eurythermal And Stenothermal Fish Species, Peter William Madden
Structural And Kinetic Characterization Of Myoglobins From Eurythermal And Stenothermal Fish Species, Peter William Madden
Electronic Theses and Dissertations
Teleost myoglobin (Mb) proteins from four fish species inhabiting different temperature environments were used to investigate the relationship between protein function and thermal stability. Mb was isolated from yellowfin tuna (homeothermal warm), mackerel (eurythermal warm), and the Antarctic teleost Notothenia coriiceps (stenothermal cold). Zebrafish (stenothermal tropical) myoglobin was expressed from cloned cDNA. N. coriiceps Mb cDNA has also been cloned, expressed at 20°C , and isolated from E. coli, but was not used in any of the functional and kinetic studies. These proteins differed in oxygen affinity, as measured by O2 dissociation rates and P50 values, and …
Vaccines For Infection Salmon Anemia Virus, Nathan Edward Charles Brown
Vaccines For Infection Salmon Anemia Virus, Nathan Edward Charles Brown
Electronic Theses and Dissertations
Infectious salmon anemia (ISA) virus is an emerging pathogen of fanned Atlantic salmon. Due to the massive economic losses inflicted by the ISA virus, effective measures to control future outbreaks are necessary. An attractive method for preventing ISA virus from infecting stocks of Atlantic salmon is vaccination. DNA vaccination is a proven cheap, effective means of protecting fish from aquatic viruses. In this thesis DNA vaccination of Atlantic salmon was investigated. Three different strains of ISA virus were cultured and purified by density gradient ultracentrifugation. ISA virus RNA was isolated and used to amplify full-length cDNAs of five ISA virus …
Identification And Partial Characterization Of A Family Of Putative Palmitoyltransferases In Dictyostelium Discoideum, Brent Elliot Wells
Identification And Partial Characterization Of A Family Of Putative Palmitoyltransferases In Dictyostelium Discoideum, Brent Elliot Wells
Electronic Theses and Dissertations
Heterotrimeric guanine nucleotide binding proteins (G-proteins) are essential components of a wide variety of eukaryotic cellular signaling pathways. Heterotrimeric G proteins consist of a 40 kDa α-subunit, a 36 kDa β-subunit and a small 8-10 kDa γ- subunit. Acting as molecular switches, G proteins relay molecular information fiom membrane bound receptors to downstream intracellular effectors. Most G-proteins require lipid modification by myristic acid and palmitic acid for proper localization and function. Protein palmitoylation is a post-translational, reversible thioester linkage of palmitic acid (C16:O) to an N- terminal cysteine residue of a substrate protein. Palmitoylation of G-proteins occurs specifically on the …
Critical Amino Acids Of The Gα2 Subunit Helical Domain In Dictyostelium Discoideum, Steven Martin Rauch
Critical Amino Acids Of The Gα2 Subunit Helical Domain In Dictyostelium Discoideum, Steven Martin Rauch
Electronic Theses and Dissertations
In Dictyostelium discoideum organism, the Gα2 subunit of the heterotrimeric G-protein signaling complex plays a pivotal role during the aggregation stage in the Dictyostelium life cycle. The biochemical functions of the G-protein complex include separation of the G-protein coupled receptor from the G-protein subunits, GDP displacement by GTP in the Gα subunit, separation of the Gα monomer from the βγ complex, GTP hydrolysis to GDP, activation of adenylyl cyclase as a downstream effector, and activation of guanylyl cyclase as a separate downstream effector. Upon release from the heterotrimer, the βγ subunits lead to downstream activation of the membrane bound adenylyl …
Analysis Of Protistan Grazing On Bioremediative Bacteria Using In Wvo Fluorescent Protein Expression And Flow Cytometry, Yutao Fu
Electronic Theses and Dissertations
Protistan bacterivory can influence the size distribution, cell structure and composition of natural bacterial communities and is of significant concern for design of bioremediation efforts, yet adequate methods for observation and modeling are lacking. In this investigation, fluorescent protein expression and flow cytometry were used to study protistan grazing on genetically modified strains of several bacterial species that have been considered for use in bioremediation. Broad-host-range plasmids were constructed and used to introduce genes encoding GFP (green fluorescent protein) or RFP (red fluorescent protein) to prey species. A heterotrophic flagellate Paraphysomonas imperforata (Hflag) served as a model predator. Predator-prey interactions …
Cold-Temperature Adaptation Of Muscle Creatine Kinase From An Antartic Teleost (Chaenocephalus Aceratus), Paul Winnard Jr.
Cold-Temperature Adaptation Of Muscle Creatine Kinase From An Antartic Teleost (Chaenocephalus Aceratus), Paul Winnard Jr.
Electronic Theses and Dissertations
The white muscle of Chuenocephulus aceratus, an Antarctic teleost of the Channicthyidae family, has a compromised glycoiytic capacity and this fish cannot depend on glycolysis for rapid ATP generation For C. aceratus, creatine kinase (CK) and phosphocreatine (PCr) reserves comprise the metabolic pathway that may supplement and overcome this deficiency in energy transduction. Two conditions, low glycolytic capacity and evolution in a chronically cold habitat (-1.86°C), give us reason to believe that C. aceratus muscle CK (MMCK) has been subjected to strong selective pressure. Thus, the hypothesis of this thesis is that MMCK fiom C. aceratus white muscle …
Detection Of Insulin Receptor, Epidermal Growth Factor Receptor, And Interleukin-6 On Individual Mouse Embryos By Immuno-Polymerase Chain Reaction, Kun Xu
Electronic Theses and Dissertations
Three series of experiments were conducted to: 1) optimize the conditions for the production of pUC19 plasmid and of biotinylated pUC19 fragments; 2) optimize the conditions for the production of protein A-streptavidin chimera (chimeric protein); and 3) detect soluble antigens [bovine serum albumin (BSA) and interleukin-6 (IL-6)] and membrane-bound antigens [insulin receptor and epidermal growth factor receptor (EGFr)] of mouse embryos by immuno-polymerase chain reaction (I-PCR). The first experimental series, which included bacterial culture, chimeric protein purification, and chimeric protein functional experiments, was performed to investigate the effects of IPTG (isopropyl-β-D-thiogalactopyranoside) induction time and temperature, bacterial culture medium, and protein …
Autoimmunity, Immune Deficiency And Cancer: Multiple Roles Of The Protein Tyrosine Phosphate Shp-1, Melissa J. Joliat
Autoimmunity, Immune Deficiency And Cancer: Multiple Roles Of The Protein Tyrosine Phosphate Shp-1, Melissa J. Joliat
Electronic Theses and Dissertations
One of a large number of mutant mice used in immunological research, the "motheaten" mouse was the first model of a specific protein tyrosine phosphatase deficiency. Mice carrying one of two allelic mutations at the "motheaten" locus have severe systemic autoimmunity and immune dysfunction as a result of mutations in the hematopoietic-cell phosphatase (Hcph) gene, which encodes the protein tyrosine phosphatase SHP-1. Studies using "motheaten" (me/me) and "viable motheaten" (mev/mev) mice have increased the understanding of numerous signaling pathways in immune and hematopoietic cells. A number of studies on SHP-1 function …