Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
A Chemoenzymatic Approach For Synthesizing Polymeric Hemoglobin, Johann P. Sigurjonsson
A Chemoenzymatic Approach For Synthesizing Polymeric Hemoglobin, Johann P. Sigurjonsson
Graduate Student Symposium
Polymerized hemoglobin (Hb) molecules have been shown to decrease previously observed adverse events associated with the administration of cell-free hemoglobin. To create these polymers, a method will be developed which employs the site specific ligation reaction of the sortase A enzyme from S. aureus. An Hb mutant (“αcpβ“) previously developed in our lab has been further modified by adding either the sortase recognition sequence, LPXTG, to the C-terminus of the α-subunit (s-αcpβ), or a tetraglycine motif, GGGG, to the N-terminus (n-αcpβ). Three types of sortase mediated ligation (SML) will be employed in this study. First, we will attempt to ligate …
Substrate Analogs For Characterizing The Substrate Tolerance Of S. Pneumoniae Srta, Orion Banks
Substrate Analogs For Characterizing The Substrate Tolerance Of S. Pneumoniae Srta, Orion Banks
Graduate Student Symposium
Bacterial sortases have been widely studied for their usefulness in protein modification, however, the variable substrate specificity and activity between homologs of these enzymes is not yet fully characterized. To attempt to further understand sorting signal recognition, we are working towards a substrate bound structure of sortase A from Streptococcus pneumoniae (SrtApneu). This enzyme displays a wide tolerance for alternate amino acids within the canonical LPXTG sorting motif. Our strategy involves a non-cleavable substrate analog that can be docked into the active site, allowing for elucidation of a structure displaying the key contacts that allow the enzyme …